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The map label for this gene is dsbE [H]

Identifier: 49474929

GI number: 49474929

Start: 151611

End: 152258

Strand: Reverse

Name: dsbE [H]

Synonym: BH01110

Alternate gene names: 49474929

Gene position: 152258-151611 (Counterclockwise)

Preceding gene: 49474930

Following gene: 49474927

Centisome position: 7.88

GC content: 33.8

Gene sequence:

>648_bases
ATGAAAATCACCCCTCCAAAACCTGAAAAAAATATATCTTTGCCTCTCTTCTTTGATCTTGTGGGACCATTTGTGTTTTT
TCTCCTTCTTCTTATGTTTTTATTCTACTTTATGCAGAGCAAACACCCCAATGATACTTCTCTTCTCCCAAACACATTGG
CTGAAAAACTGGCCCCAAAAATAAGTCTTCCTCTCTTTGATAATGAAAATTATCTCCTTAATTCAGATCAATTTAAAGGG
CGCGTAACATTGATCAATTTTTGGGGTTCTTGGTGCCCATCTTGTCGCGAGGAACATCCTCTTCTTATGAAGATTGCGCA
AGATCAACGTTTTGACCTTATTGGTATTAATTATAAAGATAATAAAGAAAATGCTCAACGCTTTCTGAATAATTTTGGTA
ATCCTTTTAAATTTATTGGCTTTGATGTTTCAGGGTACACGGCTATTAATTGGGGAGTCTATGGACCACCAGAAACCTTC
CTTCTGAATAAAGAAAACATCATTATTGCTAAACATGTCGGTCCTTTAACATGGCAGATTTATCAGAAAAAAATTCTACC
CAAAATCGAAAAAGCAATTATGACAGAAGAAATCTTAAAAAACAAAAGATTGATTTATAAAGATAATTTATCTTCTTCCC
AATATTGA

Upstream 100 bases:

>100_bases
CTGCTGTGTTTTATTGGATACATTTTTTGCAAGACTTTATACCAAAAAAAAATCCTACAACAGCTGAACAAAAAAGAGCT
GTTTCGGAAAGAAAAATATC

Downstream 100 bases:

>100_bases
ATGAGAAAGCACCAAATACAGAAAGTCTTTCTCATTCCAGCTTTCTCTTATGGTAAATCATAAAAAATTTTGCTTGTACA
TCACAAGAGAAGCGAAATTT

Product: Thiol 3A-disulfide interchange protein

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CycY [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKITPPKPEKNISLPLFFDLVGPFVFFLLLLMFLFYFMQSKHPNDTSLLPNTLAEKLAPKISLPLFDNENYLLNSDQFKG
RVTLINFWGSWCPSCREEHPLLMKIAQDQRFDLIGINYKDNKENAQRFLNNFGNPFKFIGFDVSGYTAINWGVYGPPETF
LLNKENIIIAKHVGPLTWQIYQKKILPKIEKAIMTEEILKNKRLIYKDNLSSSQY

Sequences:

>Translated_215_residues
MKITPPKPEKNISLPLFFDLVGPFVFFLLLLMFLFYFMQSKHPNDTSLLPNTLAEKLAPKISLPLFDNENYLLNSDQFKG
RVTLINFWGSWCPSCREEHPLLMKIAQDQRFDLIGINYKDNKENAQRFLNNFGNPFKFIGFDVSGYTAINWGVYGPPETF
LLNKENIIIAKHVGPLTWQIYQKKILPKIEKAIMTEEILKNKRLIYKDNLSSSQY
>Mature_215_residues
MKITPPKPEKNISLPLFFDLVGPFVFFLLLLMFLFYFMQSKHPNDTSLLPNTLAEKLAPKISLPLFDNENYLLNSDQFKG
RVTLINFWGSWCPSCREEHPLLMKIAQDQRFDLIGINYKDNKENAQRFLNNFGNPFKFIGFDVSGYTAINWGVYGPPETF
LLNKENIIIAKHVGPLTWQIYQKKILPKIEKAIMTEEILKNKRLIYKDNLSSSQY

Specific function: Required for disulfide bond formation in some periplasmic proteins. Also act as a disulfide oxidoreductase in cytochromes c biogenesis. The cysteines of apocytochromes c must be in the reduced state for covalent linkage between the two moieties to occur [

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=171, Percent_Identity=33.9181286549708, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 25060; Mature: 25060

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITPPKPEKNISLPLFFDLVGPFVFFLLLLMFLFYFMQSKHPNDTSLLPNTLAEKLAPK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
ISLPLFDNENYLLNSDQFKGRVTLINFWGSWCPSCREEHPLLMKIAQDQRFDLIGINYKD
EECEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCC
NKENAQRFLNNFGNPFKFIGFDVSGYTAINWGVYGPPETFLLNKENIIIAKHVGPLTWQI
CHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCEEEECCCCEEEEECCCCHHHHH
YQKKILPKIEKAIMTEEILKNKRLIYKDNLSSSQY
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure
MKITPPKPEKNISLPLFFDLVGPFVFFLLLLMFLFYFMQSKHPNDTSLLPNTLAEKLAPK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCC
ISLPLFDNENYLLNSDQFKGRVTLINFWGSWCPSCREEHPLLMKIAQDQRFDLIGINYKD
EECEEECCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCC
NKENAQRFLNNFGNPFKFIGFDVSGYTAINWGVYGPPETFLLNKENIIIAKHVGPLTWQI
CHHHHHHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCEEEECCCCEEEEECCCCHHHHH
YQKKILPKIEKAIMTEEILKNKRLIYKDNLSSSQY
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1850420; 12597275 [H]