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Identifier: 49474898

GI number: 49474898

Start: 102009

End: 102539

Strand: Reverse

Name: def

Synonym: BH00760

Alternate gene names: 49474898

Gene position: 102539-102009 (Counterclockwise)

Preceding gene: 49474901

Following gene: 49474897

Centisome position: 5.31

GC content: 39.55

Gene sequence:

>531_bases
ATGCCAATGAGACCTTTAGTTATTGTACCTGATCCGATTTTACGGGAAATATCCAAACCTGTTGAGTATATTGATTCAGC
TGTTCAAAAACTCGCTGATGATATGCTGGAAACTATGTATCACGCCCAAGGCGTTGGCCTTGCCGCCATTCAGATTGGCA
TACCCCTACGTATGTTGGTTTTAGATGTCTCGCGAAATGATGAACAGAAAAATCCTCTAGTTATTATCAACCCTGAAGTC
TTATGGCTTTCAGATGAGCGTAATATTTACAAAGAGGGCTGTCTTTCTATTCCAGAATATTTTGCAGAAGTCGAACGCCC
CAAACGCCTCTGTGTTCGCTATCAAAATCGTGAAGGAAAACAAACAGAAATTGAGGCAGATGATCTCCTCGCTACTTGCT
TACAGCACGAAATCGATCATTTAAACGGTCGCCTTTTTATTGATTATCTTTCAAAGATCAAACGCGATATGGTGATACGA
AAATTTAAAAAACGTGCAAAAGAAAAAAACACACAGGAAGCAGTTTTGTAA

Upstream 100 bases:

>100_bases
ATCAAACATAAAAAGTAATATAACGCTCTTACCGTAAAAAAGCGTAAGAATCTGCCATTCAATATTGACCCATAACAAAC
CATTGATTAATCTGTGCCTC

Downstream 100 bases:

>100_bases
TGGTATTACGGTTAAGTTTTATGGGGACACCTGATTTTTCTGTTCCCATTTTACAGGCTTTATTGGACGCTGGTCATGAT
ATTGCAGCTGTTTATAGCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPMRPLVIVPDPILREISKPVEYIDSAVQKLADDMLETMYHAQGVGLAAIQIGIPLRMLVLDVSRNDEQKNPLVIINPEV
LWLSDERNIYKEGCLSIPEYFAEVERPKRLCVRYQNREGKQTEIEADDLLATCLQHEIDHLNGRLFIDYLSKIKRDMVIR
KFKKRAKEKNTQEAVL

Sequences:

>Translated_176_residues
MPMRPLVIVPDPILREISKPVEYIDSAVQKLADDMLETMYHAQGVGLAAIQIGIPLRMLVLDVSRNDEQKNPLVIINPEV
LWLSDERNIYKEGCLSIPEYFAEVERPKRLCVRYQNREGKQTEIEADDLLATCLQHEIDHLNGRLFIDYLSKIKRDMVIR
KFKKRAKEKNTQEAVL
>Mature_175_residues
PMRPLVIVPDPILREISKPVEYIDSAVQKLADDMLETMYHAQGVGLAAIQIGIPLRMLVLDVSRNDEQKNPLVIINPEVL
WLSDERNIYKEGCLSIPEYFAEVERPKRLCVRYQNREGKQTEIEADDLLATCLQHEIDHLNGRLFIDYLSKIKRDMVIRK
FKKRAKEKNTQEAVL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=30.1204819277108, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1789682, Length=156, Percent_Identity=50, Blast_Score=154, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=35.5421686746988, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=26.7857142857143, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BARHE (Q6G5F0)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_032939.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q6G5F0
- SMR:   Q6G5F0
- GeneID:   2864510
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH00760
- NMPDR:   fig|283166.1.peg.67
- HOGENOM:   HBG665227
- OMA:   ETMIASE
- PhylomeDB:   Q6G5F0
- ProtClustDB:   PRK00150
- BioCyc:   BHEN283166:BH00760-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20414; Mature: 20283

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMRPLVIVPDPILREISKPVEYIDSAVQKLADDMLETMYHAQGVGLAAIQIGIPLRMLV
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHEE
LDVSRNDEQKNPLVIINPEVLWLSDERNIYKEGCLSIPEYFAEVERPKRLCVRYQNREGK
EECCCCCCCCCCEEEECCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
QTEIEADDLLATCLQHEIDHLNGRLFIDYLSKIKRDMVIRKFKKRAKEKNTQEAVL
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
PMRPLVIVPDPILREISKPVEYIDSAVQKLADDMLETMYHAQGVGLAAIQIGIPLRMLV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHEE
LDVSRNDEQKNPLVIINPEVLWLSDERNIYKEGCLSIPEYFAEVERPKRLCVRYQNREGK
EECCCCCCCCCCEEEECCEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
QTEIEADDLLATCLQHEIDHLNGRLFIDYLSKIKRDMVIRKFKKRAKEKNTQEAVL
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA