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The map label for this gene is fmt

Identifier: 49474897

GI number: 49474897

Start: 101074

End: 102009

Strand: Reverse

Name: fmt

Synonym: BH00750

Alternate gene names: 49474897

Gene position: 102009-101074 (Counterclockwise)

Preceding gene: 49474898

Following gene: 49474896

Centisome position: 5.28

GC content: 41.99

Gene sequence:

>936_bases
ATGGTATTACGGTTAAGTTTTATGGGGACACCTGATTTTTCTGTTCCCATTTTACAGGCTTTATTGGACGCTGGTCATGA
TATTGCAGCTGTTTATAGCCAACCTCCTCGCCCTGCAGGACGGCGTGGCCTTAAATTGATCCCCTCACCTGTACAAAATC
TAGCAAAAGCAAAATCTATTCCTGTCTTTACACCTCAAACACTCAAAACAGCAGAACAACAAACCAAATTTACAGAACTT
GCTGTTGATGTTGCCATTGTTGTTGCCTATGGACTCCTCTTACCGAAAACCATTCTTGAAACACCCCGTTTTGGTTGTTT
CAATGCCCATGCTTCACTCTTACCACGCTGGCGTGGTGCTGCACCTATTCAGCGTGCTATTATGGCTGGTGATAAGGAAA
CAGGGATGACGATTATGAAAATGGATGAAGGACTTGATACAGGGCCTATCGCTCTCTCTTGTGCCATTCCCATTACTGAT
AACACCACTACTAATGAACTTGCACATAAGCTTTCACATATAGGTGCAGATCTTATGATAGAAATGCTATCGGCTCTTGA
AAAAGGGCAACTTAAACTTACCGCACAATCAGGAGAAAACATAACTTATGCCTCAAAAATAAAAAAAGAAGAAACACGCA
TTGATTGGACAAAACCTGCAGAATTTATTCACAGATATATCCGCGCTCTTTCTCCTTTTCCTGGTTGCTGGTGCAACATG
AATATCGCCGGTCGAGAAGAACGTGTCAAAATTCTTGGAAGTCGCCTAACGACACGTCCTTCTCTTGAAATTGGTCGAAT
AGAGAAAGGACCAGATTCTTTACTTATCCATTGCGGACAAGGGTGTCTTGAAGTAACCTATTTGCAAAGATCTGGTGGTA
AAGTTCTTGAGTGTGCGACCTTTTTGCAAGGTGCTCATATTTCTGCTGTTTTTTAA

Upstream 100 bases:

>100_bases
GCCTTTTTATTGATTATCTTTCAAAGATCAAACGCGATATGGTGATACGAAAATTTAAAAAACGTGCAAAAGAAAAAAAC
ACACAGGAAGCAGTTTTGTA

Downstream 100 bases:

>100_bases
TATGCCACGTTTTAAACTCACTCTTGAATATGATGGCTCAAATTATGCCGGCTGGCAGCGCCAAGCAGAACTTCGTACTA
TACAGAGTGCTCTTGAACAG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MVLRLSFMGTPDFSVPILQALLDAGHDIAAVYSQPPRPAGRRGLKLIPSPVQNLAKAKSIPVFTPQTLKTAEQQTKFTEL
AVDVAIVVAYGLLLPKTILETPRFGCFNAHASLLPRWRGAAPIQRAIMAGDKETGMTIMKMDEGLDTGPIALSCAIPITD
NTTTNELAHKLSHIGADLMIEMLSALEKGQLKLTAQSGENITYASKIKKEETRIDWTKPAEFIHRYIRALSPFPGCWCNM
NIAGREERVKILGSRLTTRPSLEIGRIEKGPDSLLIHCGQGCLEVTYLQRSGGKVLECATFLQGAHISAVF

Sequences:

>Translated_311_residues
MVLRLSFMGTPDFSVPILQALLDAGHDIAAVYSQPPRPAGRRGLKLIPSPVQNLAKAKSIPVFTPQTLKTAEQQTKFTEL
AVDVAIVVAYGLLLPKTILETPRFGCFNAHASLLPRWRGAAPIQRAIMAGDKETGMTIMKMDEGLDTGPIALSCAIPITD
NTTTNELAHKLSHIGADLMIEMLSALEKGQLKLTAQSGENITYASKIKKEETRIDWTKPAEFIHRYIRALSPFPGCWCNM
NIAGREERVKILGSRLTTRPSLEIGRIEKGPDSLLIHCGQGCLEVTYLQRSGGKVLECATFLQGAHISAVF
>Mature_311_residues
MVLRLSFMGTPDFSVPILQALLDAGHDIAAVYSQPPRPAGRRGLKLIPSPVQNLAKAKSIPVFTPQTLKTAEQQTKFTEL
AVDVAIVVAYGLLLPKTILETPRFGCFNAHASLLPRWRGAAPIQRAIMAGDKETGMTIMKMDEGLDTGPIALSCAIPITD
NTTTNELAHKLSHIGADLMIEMLSALEKGQLKLTAQSGENITYASKIKKEETRIDWTKPAEFIHRYIRALSPFPGCWCNM
NIAGREERVKILGSRLTTRPSLEIGRIEKGPDSLLIHCGQGCLEVTYLQRSGGKVLECATFLQGAHISAVF

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=324, Percent_Identity=26.2345679012346, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI21614513, Length=333, Percent_Identity=26.1261261261261, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI164663775, Length=237, Percent_Identity=31.6455696202532, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=46.0264900662252, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788589, Length=293, Percent_Identity=29.0102389078498, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI133930964, Length=308, Percent_Identity=27.9220779220779, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319458, Length=270, Percent_Identity=27.037037037037, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550868, Length=309, Percent_Identity=31.0679611650485, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571984, Length=228, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585660, Length=274, Percent_Identity=22.992700729927, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_BARHE (Q6G5F1)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_032938.1
- ProteinModelPortal:   Q6G5F1
- SMR:   Q6G5F1
- GeneID:   2865898
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH00750
- NMPDR:   fig|283166.1.peg.66
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q6G5F1
- ProtClustDB:   CLSK926917
- BioCyc:   BHEN283166:BH00750-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33944; Mature: 33944

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLRLSFMGTPDFSVPILQALLDAGHDIAAVYSQPPRPAGRRGLKLIPSPVQNLAKAKSI
CEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCC
PVFTPQTLKTAEQQTKFTELAVDVAIVVAYGLLLPKTILETPRFGCFNAHASLLPRWRGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCC
APIQRAIMAGDKETGMTIMKMDEGLDTGPIALSCAIPITDNTTTNELAHKLSHIGADLMI
CHHHHHHHCCCCHHCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EMLSALEKGQLKLTAQSGENITYASKIKKEETRIDWTKPAEFIHRYIRALSPFPGCWCNM
HHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECC
NIAGREERVKILGSRLTTRPSLEIGRIEKGPDSLLIHCGQGCLEVTYLQRSGGKVLECAT
CCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHH
FLQGAHISAVF
HHCCCCEEEEC
>Mature Secondary Structure
MVLRLSFMGTPDFSVPILQALLDAGHDIAAVYSQPPRPAGRRGLKLIPSPVQNLAKAKSI
CEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCC
PVFTPQTLKTAEQQTKFTELAVDVAIVVAYGLLLPKTILETPRFGCFNAHASLLPRWRGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCC
APIQRAIMAGDKETGMTIMKMDEGLDTGPIALSCAIPITDNTTTNELAHKLSHIGADLMI
CHHHHHHHCCCCHHCEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
EMLSALEKGQLKLTAQSGENITYASKIKKEETRIDWTKPAEFIHRYIRALSPFPGCWCNM
HHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECC
NIAGREERVKILGSRLTTRPSLEIGRIEKGPDSLLIHCGQGCLEVTYLQRSGGKVLECAT
CCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCCEEEECCCCCEEEEEEECCCCCHHHHHH
FLQGAHISAVF
HHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA