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The map label for this gene is folC [H]

Identifier: 49474861

GI number: 49474861

Start: 44374

End: 45693

Strand: Reverse

Name: folC [H]

Synonym: BH00330

Alternate gene names: 49474861

Gene position: 45693-44374 (Counterclockwise)

Preceding gene: 49474862

Following gene: 49474860

Centisome position: 2.37

GC content: 39.17

Gene sequence:

>1320_bases
ATGAAAAAAAACCACGTGCAAAGGATAATCGATGATTTACTGAAAAACTATCCTAAAAAATTTGATCTTTCTTTAGAGCG
TATCCTTCGTCTTTTAGAAAGCCTTGATAATCCTCATTTAAAGCTTGCACCTGTTATTCATATTGCTGGCACAAATGGTA
AAGGATCTGCTTCAGCTATTTGTCGTGCTCTTTTGGAAAGCGCAGGCTACCGCGTTCATGTCTATAGCTCACCTCATCTG
GTTAATTGGAATGAACGCTGCCGAATCGGTCAAAGAGAAGGTGGGAAACTTGTCACAGAAGAACAATTGGCTGAAACAAT
TCAGCAAGTTGTCAAGGCGAACGGTCAAAAATTAATTACTGTTTTTGAAATTTTTACTGCTGCTGCCTTTATACTGTTTA
GCACACATCCAGCTGATGTCGTGATTTTAGAGGTTGGTTTAGGAGGACGTTTTGATGCTACAAATGTGATTAAAAAGCCA
GCTGTATCCCTTATTATGCCCATTGATTATGATCATGAGACCTTTCTTGGAAACACAATTTCACAAATCGCTTTTCAAAA
AGGGGGAATTATCAAATCGGGAGTTCCTGTGGTTATCAGTAAACAAGATCATGATGAAACCCTTTCTACTTTAATAACCC
TTACTGATAAAAATAAAGCACCTTATTTTCTGTTTGATCAAGATTATCAAAGTTATACAGAACACGGACGTATGGTCTTT
CAAAACAATCAAGGTCTCATGGATCTTCCATTGCCTAACCTGATTGGAGCGCATCAAATTGCCAATACCGGAGCTTCCCT
TGAAGCAATTTATCAAGCAGGTTTTCAACTTTCAGAAAAAACAATAAGTCACGCGCTGCAAAATATTTACTGGCCAGCAC
GAATGCAACATCTTACCCATGGAAATTTGGTTGATCAGATGTCTCCTGGTATTGATTTATGGTTAGATGGCGGTCACAAT
CCCGCTGCTGCAAAAATTGTTGCAGCAGAACTTACGAAATGGAAACAAAAATCAAACCGTCCTATTATTATGATTGTTGG
TATGCTCAATACCAAGGATGCTGTCAGTTATTTTCGTCCTTTAACAAACCTTGTCGATACAGTTTATACGATCCCGTTGA
TCAATAACAACGCAGGTATTTGTCCAACGATTTTAGCTGAAGCAGCTAGAAAAGTAGGACTTCCTGCTAACCCGCAAGCC
CATTTCCAAGAAGCCTTACAGAGAATTAACGCAGAGCATAAAGAGGTAATTGTCCTGATAGGCGGGTCCCTTTATCTTGC
CGGTGATATTTTACGTGACAACAAAACACCACCATGTTAG

Upstream 100 bases:

>100_bases
TTTACGTTTGATGATGAAATATCCCCCTGTTGTTAATCCTTCCAATACATCTTCGACCAATTCTCAGGCATCGCTTTCTA
AAGCAGAAGCTGCTTAAACT

Downstream 100 bases:

>100_bases
AAATTGATTATTCATAGAGCTTTGAAAGATAGACCTTTTGATCATGCAACTTGATTGTGCACTTTATCAACATCCTAAAC
TTATTACCGTTTTCGGCGGC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKKNHVQRIIDDLLKNYPKKFDLSLERILRLLESLDNPHLKLAPVIHIAGTNGKGSASAICRALLESAGYRVHVYSSPHL
VNWNERCRIGQREGGKLVTEEQLAETIQQVVKANGQKLITVFEIFTAAAFILFSTHPADVVILEVGLGGRFDATNVIKKP
AVSLIMPIDYDHETFLGNTISQIAFQKGGIIKSGVPVVISKQDHDETLSTLITLTDKNKAPYFLFDQDYQSYTEHGRMVF
QNNQGLMDLPLPNLIGAHQIANTGASLEAIYQAGFQLSEKTISHALQNIYWPARMQHLTHGNLVDQMSPGIDLWLDGGHN
PAAAKIVAAELTKWKQKSNRPIIMIVGMLNTKDAVSYFRPLTNLVDTVYTIPLINNNAGICPTILAEAARKVGLPANPQA
HFQEALQRINAEHKEVIVLIGGSLYLAGDILRDNKTPPC

Sequences:

>Translated_439_residues
MKKNHVQRIIDDLLKNYPKKFDLSLERILRLLESLDNPHLKLAPVIHIAGTNGKGSASAICRALLESAGYRVHVYSSPHL
VNWNERCRIGQREGGKLVTEEQLAETIQQVVKANGQKLITVFEIFTAAAFILFSTHPADVVILEVGLGGRFDATNVIKKP
AVSLIMPIDYDHETFLGNTISQIAFQKGGIIKSGVPVVISKQDHDETLSTLITLTDKNKAPYFLFDQDYQSYTEHGRMVF
QNNQGLMDLPLPNLIGAHQIANTGASLEAIYQAGFQLSEKTISHALQNIYWPARMQHLTHGNLVDQMSPGIDLWLDGGHN
PAAAKIVAAELTKWKQKSNRPIIMIVGMLNTKDAVSYFRPLTNLVDTVYTIPLINNNAGICPTILAEAARKVGLPANPQA
HFQEALQRINAEHKEVIVLIGGSLYLAGDILRDNKTPPC
>Mature_439_residues
MKKNHVQRIIDDLLKNYPKKFDLSLERILRLLESLDNPHLKLAPVIHIAGTNGKGSASAICRALLESAGYRVHVYSSPHL
VNWNERCRIGQREGGKLVTEEQLAETIQQVVKANGQKLITVFEIFTAAAFILFSTHPADVVILEVGLGGRFDATNVIKKP
AVSLIMPIDYDHETFLGNTISQIAFQKGGIIKSGVPVVISKQDHDETLSTLITLTDKNKAPYFLFDQDYQSYTEHGRMVF
QNNQGLMDLPLPNLIGAHQIANTGASLEAIYQAGFQLSEKTISHALQNIYWPARMQHLTHGNLVDQMSPGIDLWLDGGHN
PAAAKIVAAELTKWKQKSNRPIIMIVGMLNTKDAVSYFRPLTNLVDTVYTIPLINNNAGICPTILAEAARKVGLPANPQA
HFQEALQRINAEHKEVIVLIGGSLYLAGDILRDNKTPPC

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=326, Percent_Identity=29.4478527607362, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI66932990, Length=326, Percent_Identity=29.4478527607362, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788654, Length=422, Percent_Identity=29.8578199052133, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71984923, Length=473, Percent_Identity=27.906976744186, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=473, Percent_Identity=27.906976744186, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17553148, Length=473, Percent_Identity=28.3298097251586, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=29.6296296296296, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324815, Length=308, Percent_Identity=28.8961038961039, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=440, Percent_Identity=22.9545454545455, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24641571, Length=344, Percent_Identity=27.6162790697674, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24581568, Length=182, Percent_Identity=34.6153846153846, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48509; Mature: 48509

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNHVQRIIDDLLKNYPKKFDLSLERILRLLESLDNPHLKLAPVIHIAGTNGKGSASAI
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHH
CRALLESAGYRVHVYSSPHLVNWNERCRIGQREGGKLVTEEQLAETIQQVVKANGQKLIT
HHHHHHHCCCEEEEECCCCEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHH
VFEIFTAAAFILFSTHPADVVILEVGLGGRFDATNVIKKPAVSLIMPIDYDHETFLGNTI
HHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHH
SQIAFQKGGIIKSGVPVVISKQDHDETLSTLITLTDKNKAPYFLFDQDYQSYTEHGRMVF
HHHHHHCCCCEECCCCEEEECCCCHHHHHHEEEEECCCCCCEEEECCCHHHHHHCCCEEE
QNNQGLMDLPLPNLIGAHQIANTGASLEAIYQAGFQLSEKTISHALQNIYWPARMQHLTH
ECCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHC
GNLVDQMSPGIDLWLDGGHNPAAAKIVAAELTKWKQKSNRPIIMIVGMLNTKDAVSYFRP
CCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LTNLVDTVYTIPLINNNAGICPTILAEAARKVGLPANPQAHFQEALQRINAEHKEVIVLI
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
GGSLYLAGDILRDNKTPPC
CCCEEEEEEHHCCCCCCCC
>Mature Secondary Structure
MKKNHVQRIIDDLLKNYPKKFDLSLERILRLLESLDNPHLKLAPVIHIAGTNGKGSASAI
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHH
CRALLESAGYRVHVYSSPHLVNWNERCRIGQREGGKLVTEEQLAETIQQVVKANGQKLIT
HHHHHHHCCCEEEEECCCCEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHH
VFEIFTAAAFILFSTHPADVVILEVGLGGRFDATNVIKKPAVSLIMPIDYDHETFLGNTI
HHHHHHHHHHHHEECCCCCEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHH
SQIAFQKGGIIKSGVPVVISKQDHDETLSTLITLTDKNKAPYFLFDQDYQSYTEHGRMVF
HHHHHHCCCCEECCCCEEEECCCCHHHHHHEEEEECCCCCCEEEECCCHHHHHHCCCEEE
QNNQGLMDLPLPNLIGAHQIANTGASLEAIYQAGFQLSEKTISHALQNIYWPARMQHLTH
ECCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHC
GNLVDQMSPGIDLWLDGGHNPAAAKIVAAELTKWKQKSNRPIIMIVGMLNTKDAVSYFRP
CCHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
LTNLVDTVYTIPLINNNAGICPTILAEAARKVGLPANPQAHFQEALQRINAEHKEVIVLI
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEE
GGSLYLAGDILRDNKTPPC
CCCEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]