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The map label for this gene is lspA

Identifier: 49474841

GI number: 49474841

Start: 11591

End: 12082

Strand: Reverse

Name: lspA

Synonym: BH00100

Alternate gene names: 49474841

Gene position: 12082-11591 (Counterclockwise)

Preceding gene: 49474842

Following gene: 49474840

Centisome position: 0.63

GC content: 32.52

Gene sequence:

>492_bases
ATGACACGCAAATCATTTCCTTTTTTTCTTCTTGGTTTAATTCTTACAGTAGGGATTGATCAAGCAGTTAAATACTGGGT
TATGCACAATATACCCCTCGGAACAGAGACCCCGCTTTTACCTTTTCTTTCTCTCTACCATGTACGCAATTCTGGTATCG
CATTTTCTTTTTTTTCTTCTTTTTCTCATTGGGGACTTATTTTCCTCACACTCATCATTCTTATTTTCCTTTTATGGTTA
TGGAAAAATACCCAATACAATAAATCTCTAACACGTTTCGGTTTTACCCTTATTATTGGTGGTGCAATCGGTAATCTTAT
TGACCGAATCTGTTTCTATTATGTTATTGATTATATACTCTTTTATATTAATGATGTCTTTTATTTTGCCGTTTTCAATC
TTGCAGATACTTTTATCACACTTGGTGTTATTGCAATCATTATTGAAGAATTGCTTAGCTGGATTAAAAGAAAGAGTACT
TTTTCTGAATAA

Upstream 100 bases:

>100_bases
GTGGACGTGCTGATTCTCTTAATCTCGCAGTAGCAACAGCTATCATGCTTTATGAAATTCGCCGTTCTTATTTAACATTG
GAACCACGTAAGGCTGCACT

Downstream 100 bases:

>100_bases
GAATATATTTCCTTCCGAAAGCGCTTCTTTTCTATAATAGAATCAAAAGAGAGCGATATATTATCAGTTTTTATTCATAA
ATTTCGATTTAGAAGAATTT

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTRKSFPFFLLGLILTVGIDQAVKYWVMHNIPLGTETPLLPFLSLYHVRNSGIAFSFFSSFSHWGLIFLTLIILIFLLWL
WKNTQYNKSLTRFGFTLIIGGAIGNLIDRICFYYVIDYILFYINDVFYFAVFNLADTFITLGVIAIIIEELLSWIKRKST
FSE

Sequences:

>Translated_163_residues
MTRKSFPFFLLGLILTVGIDQAVKYWVMHNIPLGTETPLLPFLSLYHVRNSGIAFSFFSSFSHWGLIFLTLIILIFLLWL
WKNTQYNKSLTRFGFTLIIGGAIGNLIDRICFYYVIDYILFYINDVFYFAVFNLADTFITLGVIAIIIEELLSWIKRKST
FSE
>Mature_162_residues
TRKSFPFFLLGLILTVGIDQAVKYWVMHNIPLGTETPLLPFLSLYHVRNSGIAFSFFSSFSHWGLIFLTLIILIFLLWLW
KNTQYNKSLTRFGFTLIIGGAIGNLIDRICFYYVIDYILFYINDVFYFAVFNLADTFITLGVIAIIIEELLSWIKRKSTF
SE

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=147, Percent_Identity=39.4557823129252, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_BARHE (Q6G5A2)

Other databases:

- EMBL:   BX897699
- RefSeq:   YP_032882.1
- MEROPS:   A08.001
- GeneID:   2865603
- GenomeReviews:   BX897699_GR
- KEGG:   bhe:BH00100
- NMPDR:   fig|283166.1.peg.10
- HOGENOM:   HBG724422
- OMA:   DIHINDW
- PhylomeDB:   Q6G5A2
- ProtClustDB:   PRK14795
- BioCyc:   BHEN283166:BH00100-MONOMER
- BRENDA:   3.4.23.36
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 18979; Mature: 18848

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 108-108 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

HASH(0x2151cc64)-; HASH(0x233ac0a0)-; HASH(0x233ab2c0)-; HASH(0x233ada68)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKSFPFFLLGLILTVGIDQAVKYWVMHNIPLGTETPLLPFLSLYHVRNSGIAFSFFSS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
FSHWGLIFLTLIILIFLLWLWKNTQYNKSLTRFGFTLIIGGAIGNLIDRICFYYVIDYIL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYINDVFYFAVFNLADTFITLGVIAIIIEELLSWIKRKSTFSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
>Mature Secondary Structure 
TRKSFPFFLLGLILTVGIDQAVKYWVMHNIPLGTETPLLPFLSLYHVRNSGIAFSFFSS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
FSHWGLIFLTLIILIFLLWLWKNTQYNKSLTRFGFTLIIGGAIGNLIDRICFYYVIDYIL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FYINDVFYFAVFNLADTFITLGVIAIIIEELLSWIKRKSTFSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA