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The map label for this gene is trmH [C]

Identifier: 49474842

GI number: 49474842

Start: 12079

End: 12942

Strand: Reverse

Name: trmH [C]

Synonym: BH00110

Alternate gene names: 49474842

Gene position: 12942-12079 (Counterclockwise)

Preceding gene: 49474843

Following gene: 49474841

Centisome position: 0.67

GC content: 39.12

Gene sequence:

>864_bases
CTGGATTGCTTGAACATGTGTATGCCTAAAACCGGTAAGGTTAAAGAAATTACCTCTCTTAGCAATCCTATCATTAAAGA
CCTTAAGGCACTTAGCCAGAAAAAAAACCGTAATCGAGAAGGCCTTTTCATGGCAGAAGGGCTTAAATTAGTGATTGATG
CTCTCAACCTTGGCTGGAAAATACAAACACTCATTTTTTCTAAAAGCAAAATCAGTAACACTGCTATTGAAAACACCGCT
GCACATACTGTAGCCAATGGTGGTCTTGTCATTAAAGCCTCACAAAAGGTCATGGAATCTATCGCACGACGAGATAATCC
ACAAACAGTTATTGGTATTTTTAAACAACAGTGGCAATCACTTGAAATGATAACAGGACAGGCAGAAGATGTTTATGTCG
CACTTGATCGAGTACGTGATCCAGGAAATCTTGGTACCATCATCCGTACTGCTGATGCTGTAGGAGCTAAAGGTGTTATT
TTGATCGGCGAAACAACAGATCCTTTTTCACCTGAAACAGTCCGTGCAACAATGGGATCAATTTTCTCCGTACCACTTTA
TCGTTTTGATGAAAGCGCCTTTTTAAACTGGTCTACACACTTTAAAGGCATGATCGTTGGCACTCATTTAAAGGGATCTA
TTGATTATCGTACAATTGATTTCAAAAATGGTCCTGTTATCCTTTTAATGGGAAATGAAAAACAAGGTTTACCAGATATT
CTTGCAAATTGTTGTGATAAACTAGCACGCATTCCACAAAGTGGACGTGCTGATTCTCTTAATCTCGCAGTAGCAACAGC
TATCATGCTTTATGAAATTCGCCGTTCTTATTTAACATTGGAACCACGTAAGGCTGCACTATGA

Upstream 100 bases:

>100_bases
GTGATGAATTTATAAATCTTGGTGGTACAGTCGAATCAGGAGAACTGATCTTGCGTGAAAAAACCACCGGACGCGCTCTT
TCTACTTCTCTTTTTAGCCG

Downstream 100 bases:

>100_bases
CACGCAAATCATTTCCTTTTTTTCTTCTTGGTTTAATTCTTACAGTAGGGATTGATCAAGCAGTTAAATACTGGGTTATG
CACAATATACCCCTCGGAAC

Product: tRNA/rRNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDCLNMCMPKTGKVKEITSLSNPIIKDLKALSQKKNRNREGLFMAEGLKLVIDALNLGWKIQTLIFSKSKISNTAIENTA
AHTVANGGLVIKASQKVMESIARRDNPQTVIGIFKQQWQSLEMITGQAEDVYVALDRVRDPGNLGTIIRTADAVGAKGVI
LIGETTDPFSPETVRATMGSIFSVPLYRFDESAFLNWSTHFKGMIVGTHLKGSIDYRTIDFKNGPVILLMGNEKQGLPDI
LANCCDKLARIPQSGRADSLNLAVATAIMLYEIRRSYLTLEPRKAAL

Sequences:

>Translated_287_residues
MDCLNMCMPKTGKVKEITSLSNPIIKDLKALSQKKNRNREGLFMAEGLKLVIDALNLGWKIQTLIFSKSKISNTAIENTA
AHTVANGGLVIKASQKVMESIARRDNPQTVIGIFKQQWQSLEMITGQAEDVYVALDRVRDPGNLGTIIRTADAVGAKGVI
LIGETTDPFSPETVRATMGSIFSVPLYRFDESAFLNWSTHFKGMIVGTHLKGSIDYRTIDFKNGPVILLMGNEKQGLPDI
LANCCDKLARIPQSGRADSLNLAVATAIMLYEIRRSYLTLEPRKAAL
>Mature_287_residues
MDCLNMCMPKTGKVKEITSLSNPIIKDLKALSQKKNRNREGLFMAEGLKLVIDALNLGWKIQTLIFSKSKISNTAIENTA
AHTVANGGLVIKASQKVMESIARRDNPQTVIGIFKQQWQSLEMITGQAEDVYVALDRVRDPGNLGTIIRTADAVGAKGVI
LIGETTDPFSPETVRATMGSIFSVPLYRFDESAFLNWSTHFKGMIVGTHLKGSIDYRTIDFKNGPVILLMGNEKQGLPDI
LANCCDKLARIPQSGRADSLNLAVATAIMLYEIRRSYLTLEPRKAAL

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=34.1772151898734, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790623, Length=202, Percent_Identity=30.6930693069307, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666840, Length=286, Percent_Identity=24.4755244755245, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 31556; Mature: 31556

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCLNMCMPKTGKVKEITSLSNPIIKDLKALSQKKNRNREGLFMAEGLKLVIDALNLGWK
CCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCEE
IQTLIFSKSKISNTAIENTAAHTVANGGLVIKASQKVMESIARRDNPQTVIGIFKQQWQS
EEEEEEECHHHCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEMITGQAEDVYVALDRVRDPGNLGTIIRTADAVGAKGVILIGETTDPFSPETVRATMGS
HHHHCCCCCCEEEEEECCCCCCCCCHHEEEHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
IFSVPLYRFDESAFLNWSTHFKGMIVGTHLKGSIDYRTIDFKNGPVILLMGNEKQGLPDI
HHHCCCEEECCCCEEEECCCCCEEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCHHH
LANCCDKLARIPQSGRADSLNLAVATAIMLYEIRRSYLTLEPRKAAL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHCCC
>Mature Secondary Structure
MDCLNMCMPKTGKVKEITSLSNPIIKDLKALSQKKNRNREGLFMAEGLKLVIDALNLGWK
CCHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCEE
IQTLIFSKSKISNTAIENTAAHTVANGGLVIKASQKVMESIARRDNPQTVIGIFKQQWQS
EEEEEEECHHHCHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHH
LEMITGQAEDVYVALDRVRDPGNLGTIIRTADAVGAKGVILIGETTDPFSPETVRATMGS
HHHHCCCCCCEEEEEECCCCCCCCCHHEEEHHCCCCCEEEEECCCCCCCCCHHHHHHHHH
IFSVPLYRFDESAFLNWSTHFKGMIVGTHLKGSIDYRTIDFKNGPVILLMGNEKQGLPDI
HHHCCCEEECCCCEEEECCCCCEEEEEEEECCCEEEEEEEECCCCEEEEECCCCCCCHHH
LANCCDKLARIPQSGRADSLNLAVATAIMLYEIRRSYLTLEPRKAAL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]