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The map label for this gene is yfkN [H]

Identifier: 49186479

GI number: 49186479

Start: 3451794

End: 3452756

Strand: Direct

Name: yfkN [H]

Synonym: BAS3476

Alternate gene names: 49186479

Gene position: 3451794-3452756 (Clockwise)

Preceding gene: 49186464

Following gene: 49186480

Centisome position: 66.02

GC content: 33.64

Gene sequence:

>963_bases
TTGAATCCAAATCAAATTGTGACTTTAAACATCTTATTAACAAGTGATATACATGGTACTGTTTATCCAATTCACTACCA
AGATAACTCCAAAGCTGAAATTGGTTTAGCTAAAATTTCTACTCTTATAAAAAAAGAACGTTCCAAAAATAGAAACGTTC
TTTTAATTGATAACGGTGACTTCATACAAGGAACACCATTTACTTATCATTACGCTACTTATGAAAAAGATAAGAAAAAT
CCAATGTCTTTATTGGCGAACTATTTACGTTATGATGCTGCAGTGATTGGGAATCATGAATTTAATTACGGAATGGACGT
ATTAAACAATGTTATTTCTACTGCAAACTTCCCGTATTTATCAGCAAATATTTTAGATAAAAACAGTAAAAAACCTTATT
TCGGGAAACCATATGTAATAAAACATATTGAATCAAATATAAAAATTGCTATTTTAGGGGTGACAACGCATTATATTCCA
AATTGGGAACAACCCGATCACATTAAAGACTTACAATTTAAAGATGCATTAGAAACAACACAAAATTGGGTTTCTTACAT
ACGTGAACACGAGAAACCAGATTTACTAGTCGTAGCGTATCATGGGGGATTTGAACGGAATTTACAAACCGGAGTACCGA
CTGAAGTTTTAACAGGAGAAAACCAAGGTTATGCAATGTGCCATGAAATTGAAGGCATAGATATTTTGCTAACAGGCCAC
CAACACCGGGAAATTGCTCACACCGTAATAAACGGTGTAACAGTATTACAAACCGGATGCAATGGACACTTTGTTGGAAA
AGTAACAATAACATTCGAAAAAACAGAACAAAGTTGGATAAAAAAGAGAGCTATTCACAATTATTGCCCGTGCAAGGAAT
TGCTACGGACCAAGATATTCTTTCTTTGGTTACTGATTATGAAGAGAAAACACAGAATTGGCTCGATCAACCTATTGGGC
TAA

Upstream 100 bases:

>100_bases
ATTTACAGAATCTTTACACATTCTATACACTCACATTAAAATTCCCTTCATATATTTGATATGCTTAATTAGTAGATAAC
AATAGAAAAGGTGAATAATA

Downstream 100 bases:

>100_bases
TCTATGGGGACATGCAAATTTCAGATGCTATGCAAACTCGTTTGCAAGACCATCCTTTTATTGAATTTATAAATAAAATA
CAGATGGATATTGCTAATGT

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase, N-terminus

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYVIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFKDALETTQNWVSYIREHEKPDLLVVAYHGGFERNLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQSWIKKRAIHNYCPCKELLRTKIFFLWLLIMKRKHRIGSINLLG

Sequences:

>Translated_320_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYVIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFKDALETTQNWVSYIREHEKPDLLVVAYHGGFERNLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQSWIKKRAIHNYCPCKELLRTKIFFLWLLIMKRKHRIGSINLLG
>Mature_320_residues
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGDFIQGTPFTYHYATYEKDKKN
PMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYLSANILDKNSKKPYFGKPYVIKHIESNIKIAILGVTTHYIP
NWEQPDHIKDLQFKDALETTQNWVSYIREHEKPDLLVVAYHGGFERNLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQSWIKKRAIHNYCPCKELLRTKIFFLWLLIMKRKHRIGSINLLG

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=211, Percent_Identity=28.9099526066351, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1790658, Length=316, Percent_Identity=29.746835443038, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786687, Length=298, Percent_Identity=27.1812080536913, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 36634; Mature: 36634

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGD
CCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
FIQGTPFTYHYATYEKDKKNPMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYL
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHCCCCEE
SANILDKNSKKPYFGKPYVIKHIESNIKIAILGVTTHYIPNWEQPDHIKDLQFKDALETT
EEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
QNWVSYIREHEKPDLLVVAYHGGFERNLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEECC
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQSWIKKRAIHNYCPCKELLRTKIF
CHHHHHHHHHCCEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHH
FLWLLIMKRKHRIGSINLLG
HHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MNPNQIVTLNILLTSDIHGTVYPIHYQDNSKAEIGLAKISTLIKKERSKNRNVLLIDNGD
CCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCC
FIQGTPFTYHYATYEKDKKNPMSLLANYLRYDAAVIGNHEFNYGMDVLNNVISTANFPYL
EEECCCCEEEEEECCCCCCCHHHHHHHHHHHCEEEECCCCCCCCHHHHHHHHHHCCCCEE
SANILDKNSKKPYFGKPYVIKHIESNIKIAILGVTTHYIPNWEQPDHIKDLQFKDALETT
EEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
QNWVSYIREHEKPDLLVVAYHGGFERNLQTGVPTEVLTGENQGYAMCHEIEGIDILLTGH
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCEEEEEECCCEEEEEECC
QHREIAHTVINGVTVLQTGCNGHFVGKVTITFEKTEQSWIKKRAIHNYCPCKELLRTKIF
CHHHHHHHHHCCEEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHH
FLWLLIMKRKHRIGSINLLG
HHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]