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The map label for this gene is yfkN [H]

Identifier: 49186480

GI number: 49186480

Start: 3452570

End: 3453376

Strand: Direct

Name: yfkN [H]

Synonym: BAS3477

Alternate gene names: 49186480

Gene position: 3452570-3453376 (Clockwise)

Preceding gene: 49186479

Following gene: 49186482

Centisome position: 66.03

GC content: 33.83

Gene sequence:

>807_bases
ATGCAATGGACACTTTGTTGGAAAAGTAACAATAACATTCGAAAAAACAGAACAAAGTTGGATAAAAAAGAGAGCTATTC
ACAATTATTGCCCGTGCAAGGAATTGCTACGGACCAAGATATTCTTTCTTTGGTTACTGATTATGAAGAGAAAACACAGA
ATTGGCTCGATCAACCTATTGGGCTAATCTATGGGGACATGCAAATTTCAGATGCTATGCAAACTCGTTTGCAAGACCAT
CCTTTTATTGAATTTATAAATAAAATACAGATGGATATTGCTAATGTTTCTATTTCTTGTACAAGCTTATTCCATAATAC
ATCTCCAGGTTTTCCGACATATGTAACAATGCGTGACATCGTTTCCAATTATATTTATCCAAATACATTAAAAGTAATTA
GAATAACTGGAGCAGATATAAAAGATGCTCTTGAACTCTCTGCTTCTTATTTCACATTAAAAGAAGATGGAACCATCATC
GTAAACCCTAGCTACATAGAACCAAAACCGCAACACTATAATTACGATATGTGGGAAGGGATTTCATACGTATTAAATAT
TTCCAAACCAATTGGCGAAAGAGTAGAATCTTTACAGTATAAAGGCACTCCTCTTAATATGAATGAAGAATATGAAGTCG
TTATGAATAATTATCGCGCGAGCGGCGGGGGGAACTTCTTTATGTTCCAAAATAAACCTGTAATAAAAGACATACCAACA
GATATGTCTGAACTTATCGCTAACTATATATTGAAGCACAAAAAGATAGAAGCAACGGTTAATAACAACTGGAAAGTTAT
TAACTAA

Upstream 100 bases:

>100_bases
TGTGCCATGAAATTGAAGGCATAGATATTTTGCTAACAGGCCACCAACACCGGGAAATTGCTCACACCGTAATAAACGGT
GTAACAGTATTACAAACCGG

Downstream 100 bases:

>100_bases
CGATTATAATAAATAAAAAAGATGGGCTTCGCCCATCTTTTAGTTTAATTTTGCATTATCTTGCTTTTTTAAAGCTTTTA
ATTTCGCCGGTGTAACATCA

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase, C-terminus

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQWTLCWKSNNNIRKNRTKLDKKESYSQLLPVQGIATDQDILSLVTDYEEKTQNWLDQPIGLIYGDMQISDAMQTRLQDH
PFIEFINKIQMDIANVSISCTSLFHNTSPGFPTYVTMRDIVSNYIYPNTLKVIRITGADIKDALELSASYFTLKEDGTII
VNPSYIEPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKGTPLNMNEEYEVVMNNYRASGGGNFFMFQNKPVIKDIPT
DMSELIANYILKHKKIEATVNNNWKVIN

Sequences:

>Translated_268_residues
MQWTLCWKSNNNIRKNRTKLDKKESYSQLLPVQGIATDQDILSLVTDYEEKTQNWLDQPIGLIYGDMQISDAMQTRLQDH
PFIEFINKIQMDIANVSISCTSLFHNTSPGFPTYVTMRDIVSNYIYPNTLKVIRITGADIKDALELSASYFTLKEDGTII
VNPSYIEPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKGTPLNMNEEYEVVMNNYRASGGGNFFMFQNKPVIKDIPT
DMSELIANYILKHKKIEATVNNNWKVIN
>Mature_268_residues
MQWTLCWKSNNNIRKNRTKLDKKESYSQLLPVQGIATDQDILSLVTDYEEKTQNWLDQPIGLIYGDMQISDAMQTRLQDH
PFIEFINKIQMDIANVSISCTSLFHNTSPGFPTYVTMRDIVSNYIYPNTLKVIRITGADIKDALELSASYFTLKEDGTII
VNPSYIEPKPQHYNYDMWEGISYVLNISKPIGERVESLQYKGTPLNMNEEYEVVMNNYRASGGGNFFMFQNKPVIKDIPT
DMSELIANYILKHKKIEATVNNNWKVIN

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=277, Percent_Identity=24.187725631769, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 30932; Mature: 30932

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQWTLCWKSNNNIRKNRTKLDKKESYSQLLPVQGIATDQDILSLVTDYEEKTQNWLDQPI
CCEEEEECCCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
GLIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCTSLFHNTSPGFPTYVTMRDI
HEEECCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEHHHH
VSNYIYPNTLKVIRITGADIKDALELSASYFTLKEDGTIIVNPSYIEPKPQHYNYDMWEG
HHHCCCCCCEEEEEEECCCHHHHHHHHHHEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
ISYVLNISKPIGERVESLQYKGTPLNMNEEYEVVMNNYRASGGGNFFMFQNKPVIKDIPT
HHHEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHEECEECCCCCCEEEEECCCCHHCCCC
DMSELIANYILKHKKIEATVNNNWKVIN
CHHHHHHHHHHHHHEEEEEECCCEEEEC
>Mature Secondary Structure
MQWTLCWKSNNNIRKNRTKLDKKESYSQLLPVQGIATDQDILSLVTDYEEKTQNWLDQPI
CCEEEEECCCCCCCHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCH
GLIYGDMQISDAMQTRLQDHPFIEFINKIQMDIANVSISCTSLFHNTSPGFPTYVTMRDI
HEEECCEEHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEHHHH
VSNYIYPNTLKVIRITGADIKDALELSASYFTLKEDGTIIVNPSYIEPKPQHYNYDMWEG
HHHCCCCCCEEEEEEECCCHHHHHHHHHHEEEEECCCEEEECCCCCCCCCCCCCCHHHHH
ISYVLNISKPIGERVESLQYKGTPLNMNEEYEVVMNNYRASGGGNFFMFQNKPVIKDIPT
HHHEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHEECEECCCCCCEEEEECCCCHHCCCC
DMSELIANYILKHKKIEATVNNNWKVIN
CHHHHHHHHHHHHHEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]