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The map label for this gene is bsdA [H]

Identifier: 49186043

GI number: 49186043

Start: 3010896

End: 3011768

Strand: Direct

Name: bsdA [H]

Synonym: BAS3038

Alternate gene names: 49186043

Gene position: 3010896-3011768 (Clockwise)

Preceding gene: 49186037

Following gene: 49186045

Centisome position: 57.58

GC content: 35.05

Gene sequence:

>873_bases
ATGGATATAAGAAAACTTCGTTACTTTATTGTAATCGCTGAAGAAAAACAGCTTACCCGTGCTGCCCAACGACTTCATAT
GGCACAGCCACCTCTAAGTAGACAACTATCGCTACTAGAACAAGAATTATCAGTAAACCTCTTTGAAAGAAACGGCCGAA
ATATGCTTTTGACTGAAGAAGGAAAAATTTTATATATGAAAGCAAAAAACATTATTCTTCAATTAGAAGAAACTATTACG
GAGATTAAAGATACCAGTGAAGGGCTAAGGGGCAAGCTATCTATTGGAGCATTTCATTCTTGCATTCCTTTTCTTTCTGA
AAAAATACGCTATTTTCAAGATAACTATCCAAATGTGAACTTAAAAATATGGGAAGGTGGCCCTTCATACTTAATAGAGC
AATTAGAAAATCGGCATATCGAACTCGCTATCTTGCGTACACCTTTTAATAAAGATGTTTTTTCTAGTATTTGTTTATCA
AAGGAACCTTTCGTATTAGTCATGCCTGCTAAATGGGAACTATATCGTTCTCACCTCCCAATCCCTCTAACCGAATTAAG
CCATATTCCCCTTTTACTTTTACATAAAGATAAAGAAAATAGCTATCATCAGCTCGTCATCGATGAATGCAAGCACCTTG
GTATAACTTTAAATATAGCATGTGAATGTCCTGACTCAGCATTTATTCTTATGCTTATTATGACTGGCATCGGTGCCTCT
ATCTTGCCGAAAAGTGCTATATCTCATATCCCGCAAGAATTTATAAAAATAGTAGATTTAATAGATTTCCCTTATGAGTC
TGAATCATCAATTATATGGCTGAAAGATCGGAAGCTTTCTAAAAGAGCGCAACGATTTATAGAATGTACATAA

Upstream 100 bases:

>100_bases
TTTTAAAACTTTTTGTTTATGATATCATGTGGGAGATTATATGAAAACTATCTATAAGGTTGTTTTCCCATACAAAAATG
GTATAGAAAGGGACTTTGAC

Downstream 100 bases:

>100_bases
ATGAATTTTAGAGGTGTAGCGATGTATTTCTACGATTCTTCACCACTATTACCTCGTTTATCAAATTATTTCTTCTATTA
TTATTGAACATGTTATATTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MDIRKLRYFIVIAEEKQLTRAAQRLHMAQPPLSRQLSLLEQELSVNLFERNGRNMLLTEEGKILYMKAKNIILQLEETIT
EIKDTSEGLRGKLSIGAFHSCIPFLSEKIRYFQDNYPNVNLKIWEGGPSYLIEQLENRHIELAILRTPFNKDVFSSICLS
KEPFVLVMPAKWELYRSHLPIPLTELSHIPLLLLHKDKENSYHQLVIDECKHLGITLNIACECPDSAFILMLIMTGIGAS
ILPKSAISHIPQEFIKIVDLIDFPYESESSIIWLKDRKLSKRAQRFIECT

Sequences:

>Translated_290_residues
MDIRKLRYFIVIAEEKQLTRAAQRLHMAQPPLSRQLSLLEQELSVNLFERNGRNMLLTEEGKILYMKAKNIILQLEETIT
EIKDTSEGLRGKLSIGAFHSCIPFLSEKIRYFQDNYPNVNLKIWEGGPSYLIEQLENRHIELAILRTPFNKDVFSSICLS
KEPFVLVMPAKWELYRSHLPIPLTELSHIPLLLLHKDKENSYHQLVIDECKHLGITLNIACECPDSAFILMLIMTGIGAS
ILPKSAISHIPQEFIKIVDLIDFPYESESSIIWLKDRKLSKRAQRFIECT
>Mature_290_residues
MDIRKLRYFIVIAEEKQLTRAAQRLHMAQPPLSRQLSLLEQELSVNLFERNGRNMLLTEEGKILYMKAKNIILQLEETIT
EIKDTSEGLRGKLSIGAFHSCIPFLSEKIRYFQDNYPNVNLKIWEGGPSYLIEQLENRHIELAILRTPFNKDVFSSICLS
KEPFVLVMPAKWELYRSHLPIPLTELSHIPLLLLHKDKENSYHQLVIDECKHLGITLNIACECPDSAFILMLIMTGIGAS
ILPKSAISHIPQEFIKIVDLIDFPYESESSIIWLKDRKLSKRAQRFIECT

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=27.3356401384083, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=27, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI2367136, Length=252, Percent_Identity=26.984126984127, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788297, Length=253, Percent_Identity=29.6442687747036, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI157672245, Length=243, Percent_Identity=27.1604938271605, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790399, Length=254, Percent_Identity=25.5905511811024, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788748, Length=283, Percent_Identity=23.6749116607774, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790208, Length=257, Percent_Identity=25.2918287937743, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787601, Length=174, Percent_Identity=25.2873563218391, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=21.259842519685, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788887, Length=170, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787530, Length=299, Percent_Identity=20.4013377926421, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788626, Length=252, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786401, Length=141, Percent_Identity=31.9148936170213, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789173, Length=126, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=22.6351351351351, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=26.7206477732794, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082184, Length=147, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33544; Mature: 33544

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRKLRYFIVIAEEKQLTRAAQRLHMAQPPLSRQLSLLEQELSVNLFERNGRNMLLTEE
CCHHEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECC
GKILYMKAKNIILQLEETITEIKDTSEGLRGKLSIGAFHSCIPFLSEKIRYFQDNYPNVN
CCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEE
LKIWEGGPSYLIEQLENRHIELAILRTPFNKDVFSSICLSKEPFVLVMPAKWELYRSHLP
EEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHCCCC
IPLTELSHIPLLLLHKDKENSYHQLVIDECKHLGITLNIACECPDSAFILMLIMTGIGAS
CCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCHH
ILPKSAISHIPQEFIKIVDLIDFPYESESSIIWLKDRKLSKRAQRFIECT
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MDIRKLRYFIVIAEEKQLTRAAQRLHMAQPPLSRQLSLLEQELSVNLFERNGRNMLLTEE
CCHHEEEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCEEEEECC
GKILYMKAKNIILQLEETITEIKDTSEGLRGKLSIGAFHSCIPFLSEKIRYFQDNYPNVN
CCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEE
LKIWEGGPSYLIEQLENRHIELAILRTPFNKDVFSSICLSKEPFVLVMPAKWELYRSHLP
EEEECCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHCCCC
IPLTELSHIPLLLLHKDKENSYHQLVIDECKHLGITLNIACECPDSAFILMLIMTGIGAS
CCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHCCHH
ILPKSAISHIPQEFIKIVDLIDFPYESESSIIWLKDRKLSKRAQRFIECT
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]