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The map label for this gene is lutR [H]

Identifier: 49184233

GI number: 49184233

Start: 1262253

End: 1262909

Strand: Direct

Name: lutR [H]

Synonym: BAS1215

Alternate gene names: 49184233

Gene position: 1262253-1262909 (Clockwise)

Preceding gene: 49184232

Following gene: 49184234

Centisome position: 24.14

GC content: 35.92

Gene sequence:

>657_bases
ATGATTAAAAATGGTACGTTAAAACCTGGTGACAAACTACTTCCTGTACATCAATTAGCTGAGCAGTTTCAAGTTGGCAG
ATCTGCTGTTCGTGAAGCGCTAAGTGCGCTAAGAGCGATGGGCTTAATTGAGATGAAACAAGGAGAAGGTACATATGTGA
AGAATTTCGATTCTTCTTCATTAACAAAATCATTAAACAATAGATTATTAATGAAGAAAGAAGATATTTTGAATTTATTA
GAAGTACGAAAGGTGCTTGAAGTGGGGGCAGTTCGAGCAGCTGCGGCAAAACGTACGGAAGCTAATTTGCAAAATATGAA
GCATTGGTTAGATGAAATGGCAAAGAGCATTGGAGATGAAAAAGCTGGTGAAAAAGCAGATTTTCAGTTCCATATGGGAA
TTGCAGAGTCTTCGCATAATAACATTTTACTTGAACTCATGAATCATGTTTCAGAGATGATTGCTGAAACGATTGGTGAA
TCAAGACGCATTATTTTATATGGTGAACAAACAACATCAGAACGACTTATAGAAGAGCATCAAGTTATATATGATGCAGT
GTTAAAGCAAGATGTAGAATTAGCGCAGCAAGCAATGCTAGATCATTTAACAAATGTAGAACATATTGTCACAGGTAGAA
ACGATATAAATTCGTAA

Upstream 100 bases:

>100_bases
TTTCAATTGTTGTTTGGAAGATACTGAGGTGATTTAACTGGAGTACAAATCTATTAAACCGAAAAAGATTTACGAAGAAG
TATCTGAAGCTATTTTAACA

Downstream 100 bases:

>100_bases
TTTAATCTTTTAAAAATCTAATTTTCTGATAAAATAGAGAGAGATTAAGTAAGCGTTTTCTTTAGAAGAGGGAGAGGATA
GGCCATGGTTGAGCCTACTC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEANLQNMKHWLDEMAKSIGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGRNDINS

Sequences:

>Translated_218_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEANLQNMKHWLDEMAKSIGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGRNDINS
>Mature_218_residues
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSSLTKSLNNRLLMKKEDILNLL
EVRKVLEVGAVRAAAAKRTEANLQNMKHWLDEMAKSIGDEKAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGE
SRRIILYGEQTTSERLIEEHQVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGRNDINS

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=213, Percent_Identity=31.924882629108, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1790032, Length=219, Percent_Identity=28.310502283105, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1789353, Length=212, Percent_Identity=28.3018867924528, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790780, Length=213, Percent_Identity=30.0469483568075, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI48994955, Length=205, Percent_Identity=28.780487804878, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789621, Length=217, Percent_Identity=25.3456221198157, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24459; Mature: 24459

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNNRLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEANLQNMKHWLDEMAKSIGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGESRRIILYGEQTTSERLIEEH
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
QVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGRNDINS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure
MIKNGTLKPGDKLLPVHQLAEQFQVGRSAVREALSALRAMGLIEMKQGEGTYVKNFDSSS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCCHHH
LTKSLNNRLLMKKEDILNLLEVRKVLEVGAVRAAAAKRTEANLQNMKHWLDEMAKSIGDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KAGEKADFQFHMGIAESSHNNILLELMNHVSEMIAETIGESRRIILYGEQTTSERLIEEH
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
QVIYDAVLKQDVELAQQAMLDHLTNVEHIVTGRNDINS
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]