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The map label for this gene is mls [H]

Identifier: 49184071

GI number: 49184071

Start: 1104569

End: 1106158

Strand: Direct

Name: mls [H]

Synonym: BAS1051

Alternate gene names: 49184071

Gene position: 1104569-1106158 (Clockwise)

Preceding gene: 49184070

Following gene: 49184072

Centisome position: 21.13

GC content: 37.99

Gene sequence:

>1590_bases
ATGTCGACGCAAACTTCACGGGTTACACTCGTGGGAGAAATGTTACCAGCGTACAACGAAATATTGACACCTGAGGCGCT
TAGTTTTTTAAAGGAACTACATGAAAATTTTAATGAGCGCCGCATAGAACTTTTACAAAAGCGTATGAAAAAACAACAGA
AAATTGATGCAGGAGAGTTTCCGAAGTTTTTAGAAGAAACAAAGCGTATACGTGAAGCGGATTGGACAATTGCTAAATTG
CCAAAAGATTTAGAGGATCGCCGCGTAGAGATTACTGGTCCAGTAGATAGAAAGATGGTTATTAACGCTTTAAATTCGGG
AGCACATCTTTTTATGGCGGATTTTGAAGATTCGAATTCACCAACTTGGGAAAATGCAATTGAAGGTCAAATAAACTTAC
GAGATGCAGTAAAGGGAACGATTTCACATAAAAATGAAAACGGAAAAGAATATCGTTTAAATAGTAAAACAGCTGTATTA
ATTGTGCGTCCAAGGGGATGGCATTTAGAAGAAAAACATATGCAAGTTGACGGAAAAAATATGTCTGGTAGCTTAGTAGA
TTTCGGACTTTATTTTTTCCATAATGCGAAAGCTCTTTTAGAAAAAGGAAGCGGTCCATACTTTTACTTACCGAAAATGG
AAAGCTATTTAGAAGCAAGATTATGGAATGATGTTTTCGTATTTGCTCAAAAATATATCGGTATTCCAAATGGAACGATC
AAAGCAACGGTGTTACTGGAAACGATTCACGCTTCGTTTGAAATGGATGAAATTTTGTATGAGCTTAAAGATCATTCTGC
TGGATTAAATTGCGGAAGATGGGATTATATTTTTAGTTTTCTAAAAGCTTTCCGTAATCATAATGAATTTTTACTTCCTG
ATAGAGCGCAAGTCACGATGACGGCGCCGTTTATGCGCGCGTATTCTTTGAAAGTCATTCAAACTTGTCACCGCCGTAAT
GCACCAGCTATTGGAGGGATGGCAGCACAAATTCCGATTAAAAACAATCCTGAAGCGAATGAAGCTGCTTTTGAAAAAGT
GCGTGCTGATAAGGAGCGAGAAGCTTTAGACGGTCATGACGGGACTTGGGTTGCCCACCCGGGACTTGTGCCAGTCGCGA
TGGAAGTATTTAATCACATTATGAAAACACCGAATCAAATTTTTAGAAAACGAGAAGAAATACATGTTACAGAAAAGGAT
TTATTAGAAGTACCAGTGGGAACGATTACAGAAGAGGGTCTTCGTATGAATATTAGCGTAGGCATTCAATATATCGCATC
TTGGTTAAGCGGACGGGGAGCAGCACCAATTTATAACTTAATGGAAGATGCGGCAACAGCTGAAATCTCAAGAGCACAAG
TATGGCAATGGATTCGCCATGAAGGTGGAAAATTAAATGATGGCCGTAATATTACACTTGAATTAATGGAAGAGTTAAAA
GAAGAAGAGTTAGCAAAAATAGAAAGAGAGATTGGTAAAGAAGCCTTTAAGAAAGGGAGATTCCAAGAGGCGACAACGTT
GTTTACAAATCTCGTTCGGAATGATGAATTCGTACCATTTTTAACATTACCAGGTTATGAAATTTTATAA

Upstream 100 bases:

>100_bases
TTGAATTTTCAAAAAAAACTTCCTTTTTTGAAGATTTGTTATATAATATAAAACATAAAATCAAATCTGTTATAAGACAG
TTTAAGAAGGGGATGCTAAT

Downstream 100 bases:

>100_bases
AAAGTGAAAAGGGGATGGAACAAATGAAAAACGAAAGAATCGAGAAATTACAAGAGAGCTGGGAATTAGATACGCGTTGG
AAAGGGATTACACGTCCATA

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSTQTSRVTLVGEMLPAYNEILTPEALSFLKELHENFNERRIELLQKRMKKQQKIDAGEFPKFLEETKRIREADWTIAKL
PKDLEDRRVEITGPVDRKMVINALNSGAHLFMADFEDSNSPTWENAIEGQINLRDAVKGTISHKNENGKEYRLNSKTAVL
IVRPRGWHLEEKHMQVDGKNMSGSLVDFGLYFFHNAKALLEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYIGIPNGTI
KATVLLETIHASFEMDEILYELKDHSAGLNCGRWDYIFSFLKAFRNHNEFLLPDRAQVTMTAPFMRAYSLKVIQTCHRRN
APAIGGMAAQIPIKNNPEANEAAFEKVRADKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREEIHVTEKD
LLEVPVGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEGGKLNDGRNITLELMEELK
EEELAKIEREIGKEAFKKGRFQEATTLFTNLVRNDEFVPFLTLPGYEIL

Sequences:

>Translated_529_residues
MSTQTSRVTLVGEMLPAYNEILTPEALSFLKELHENFNERRIELLQKRMKKQQKIDAGEFPKFLEETKRIREADWTIAKL
PKDLEDRRVEITGPVDRKMVINALNSGAHLFMADFEDSNSPTWENAIEGQINLRDAVKGTISHKNENGKEYRLNSKTAVL
IVRPRGWHLEEKHMQVDGKNMSGSLVDFGLYFFHNAKALLEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYIGIPNGTI
KATVLLETIHASFEMDEILYELKDHSAGLNCGRWDYIFSFLKAFRNHNEFLLPDRAQVTMTAPFMRAYSLKVIQTCHRRN
APAIGGMAAQIPIKNNPEANEAAFEKVRADKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREEIHVTEKD
LLEVPVGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEGGKLNDGRNITLELMEELK
EEELAKIEREIGKEAFKKGRFQEATTLFTNLVRNDEFVPFLTLPGYEIL
>Mature_528_residues
STQTSRVTLVGEMLPAYNEILTPEALSFLKELHENFNERRIELLQKRMKKQQKIDAGEFPKFLEETKRIREADWTIAKLP
KDLEDRRVEITGPVDRKMVINALNSGAHLFMADFEDSNSPTWENAIEGQINLRDAVKGTISHKNENGKEYRLNSKTAVLI
VRPRGWHLEEKHMQVDGKNMSGSLVDFGLYFFHNAKALLEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYIGIPNGTIK
ATVLLETIHASFEMDEILYELKDHSAGLNCGRWDYIFSFLKAFRNHNEFLLPDRAQVTMTAPFMRAYSLKVIQTCHRRNA
PAIGGMAAQIPIKNNPEANEAAFEKVRADKEREALDGHDGTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREEIHVTEKDL
LEVPVGTITEEGLRMNISVGIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEGGKLNDGRNITLELMEELKE
EELAKIEREIGKEAFKKGRFQEATTLFTNLVRNDEFVPFLTLPGYEIL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=512, Percent_Identity=55.2734375, Blast_Score=574, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI17561814, Length=525, Percent_Identity=56, Blast_Score=590, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI71982926, Length=418, Percent_Identity=58.8516746411483, Blast_Score=507, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6324212, Length=515, Percent_Identity=51.4563106796116, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322222, Length=516, Percent_Identity=51.3565891472868, Blast_Score=521, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 60569; Mature: 60438

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQTSRVTLVGEMLPAYNEILTPEALSFLKELHENFNERRIELLQKRMKKQQKIDAGEF
CCCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PKFLEETKRIREADWTIAKLPKDLEDRRVEITGPVDRKMVINALNSGAHLFMADFEDSNS
HHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWENAIEGQINLRDAVKGTISHKNENGKEYRLNSKTAVLIVRPRGWHLEEKHMQVDGKN
CCCHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHEECCCC
MSGSLVDFGLYFFHNAKALLEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYIGIPNGTI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
KATVLLETIHASFEMDEILYELKDHSAGLNCGRWDYIFSFLKAFRNHNEFLLPDRAQVTM
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEE
TAPFMRAYSLKVIQTCHRRNAPAIGGMAAQIPIKNNPEANEAAFEKVRADKEREALDGHD
ECHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCC
GTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREEIHVTEKDLLEVPVGTITEEGLRMNISV
CCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEHHH
GIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEGGKLNDGRNITLELMEELK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EEELAKIEREIGKEAFKKGRFQEATTLFTNLVRNDEFVPFLTLPGYEIL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
STQTSRVTLVGEMLPAYNEILTPEALSFLKELHENFNERRIELLQKRMKKQQKIDAGEF
CCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PKFLEETKRIREADWTIAKLPKDLEDRRVEITGPVDRKMVINALNSGAHLFMADFEDSNS
HHHHHHHHHHHHHCCHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCCC
PTWENAIEGQINLRDAVKGTISHKNENGKEYRLNSKTAVLIVRPRGWHLEEKHMQVDGKN
CCCHHHCCCCEEHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHHEECCCC
MSGSLVDFGLYFFHNAKALLEKGSGPYFYLPKMESYLEARLWNDVFVFAQKYIGIPNGTI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCE
KATVLLETIHASFEMDEILYELKDHSAGLNCGRWDYIFSFLKAFRNHNEFLLPDRAQVTM
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEE
TAPFMRAYSLKVIQTCHRRNAPAIGGMAAQIPIKNNPEANEAAFEKVRADKEREALDGHD
ECHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCC
GTWVAHPGLVPVAMEVFNHIMKTPNQIFRKREEIHVTEKDLLEVPVGTITEEGLRMNISV
CCEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEHHH
GIQYIASWLSGRGAAPIYNLMEDAATAEISRAQVWQWIRHEGGKLNDGRNITLELMEELK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EEELAKIEREIGKEAFKKGRFQEATTLFTNLVRNDEFVPFLTLPGYEIL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA