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The map label for this gene is lmrB [H]

Identifier: 49183992

GI number: 49183992

Start: 1025155

End: 1026603

Strand: Direct

Name: lmrB [H]

Synonym: BAS0971

Alternate gene names: 49183992

Gene position: 1025155-1026603 (Clockwise)

Preceding gene: 49183991

Following gene: 49183996

Centisome position: 19.61

GC content: 38.99

Gene sequence:

>1449_bases
ATGAATGCTCAACCTGCATCTGTTTCTTCGCAGTCAAAGGAGTTTGACAATGTGAAAAGACTCCCCATTTTAATATCGAT
GATTATTGGGGCGTTTTTTACCATTTTGAATGAAACATTATTGAACGTTGCTTTTCCGCAATTGATGATTGAGTTGAATG
TAACACCGTCGACTCTTCAATGGCTTTCAACAGGGTACATGCTTGTAGTTGCTGTATTAATTCCAGCTTCTGCTTTATTA
GTACAATGGTTTACAACAAGACAAGTATTCATAGGTGCTATGGTAGTCTTTACTTTCGGTACACTTGTAAGTGCAATTGC
GCCTGGATTTTCTATACTATTAATGGGGCGCTTGTTACAAGCGGCGGGTACGGGATTAATGATGCCCGTATTAATGAATA
CGATTCTTCTTCTATATCCTCCTGAAAAGAGAGGAGCGGCAATGGGGAGTATTGGGCTTGTAATCATGTTCGCTCCTGCC
ATTGGACCGACACTTTCAGGTATTATTTTGGAAACGTTGAACTGGAGATGGCTATTTTATATCGTTTTGCCATTCGCAAT
TTTTTCTATCGTTTTCGCGTTTATTTATTTAAAAAATGTCTCAGAGCCTACTAAACCAAAGGTAGATGTATTATCTATTC
TTTTATCTACAATTGGATTTGGCGGGATTGTTTATGGATTCAGTAGCTCAGGTGAAGGTTGGGATAGTTTCCAGGTGTAT
GGAATCATTCTAATTGGTCTTGTAGCACTTCTATTTTTCGTATTACGTCAATTGAAATTGAAAGAGCCATTACTAGATCT
TAGTGCATTTAAATATCCGATGTTTACGTTAACGACAATTTTATTGACGATCATGATGATGACGATGTTCTCGACAATGA
CGTTACTACCATTTCTATTCCAAGGTGCTTTAGGTTTAACTGTTTATGCAACTGGTCTTATCATGCTTCCTGGTAGTCTA
TTGAATGGTCTGCTTTCGCCAGTTTCAGGGAAGCTCTTTGATAAATTTGGTCCAAGAGCGCTTATTATTCCTGGGACGCT
GTTATTAGCAAGTGTGATGTGGTTCTTTACACAAGTAACGGCAGATACGTCTAAAATAACTTTCATCCTATTACATGTCA
CTATGATGGTATCCATTTCAATGATTATGATGCCGGCGCAAACGAACGGTTTGAACCAACTTCCGAAGCGATTTTATCCT
CACGGAACAGCTATTTTGAATACTCTATCACAAGTAGCGGGTGCGGTAGGAGTTGCGTTCTTTATTAGCGTTATGACTTC
CGGACAGAAGAAATATTTGGATGCATCTTCAAATCCGACTGACCCAGCTCAATTGACAGAAGCAATGGTTTCAGGCGTGC
ATAACGCATTTACAATTGGCTTTGGTTTGGCACTACTTGCAGTTGTTGTAGCTTTGTTTATAAAACGTTCGCAGACTTCA
GAAAGTTAA

Upstream 100 bases:

>100_bases
GTTGTGCTGTATCGTTTTCAATTTGGTACGGGCGTTTCTACGTTAAATATGACTAGCAAAGGTAATAAAAATTTATAAAA
GAATACAAGGAGGTTTATGT

Downstream 100 bases:

>100_bases
GGGGCGAAGTAAAGAGGGTATGCCGAGGGCTACCCTCTTTATTATTTTGTTCCTACATAGTAATTTGATTTGGGTTCCTT
CTTATTAAAATAGTATTTTT

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNAQPASVSSQSKEFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQWLSTGYMLVVAVLIPASALL
VQWFTTRQVFIGAMVVFTFGTLVSAIAPGFSILLMGRLLQAAGTGLMMPVLMNTILLLYPPEKRGAAMGSIGLVIMFAPA
IGPTLSGIILETLNWRWLFYIVLPFAIFSIVFAFIYLKNVSEPTKPKVDVLSILLSTIGFGGIVYGFSSSGEGWDSFQVY
GIILIGLVALLFFVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLFQGALGLTVYATGLIMLPGSL
LNGLLSPVSGKLFDKFGPRALIIPGTLLLASVMWFFTQVTADTSKITFILLHVTMMVSISMIMMPAQTNGLNQLPKRFYP
HGTAILNTLSQVAGAVGVAFFISVMTSGQKKYLDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTS
ES

Sequences:

>Translated_482_residues
MNAQPASVSSQSKEFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQWLSTGYMLVVAVLIPASALL
VQWFTTRQVFIGAMVVFTFGTLVSAIAPGFSILLMGRLLQAAGTGLMMPVLMNTILLLYPPEKRGAAMGSIGLVIMFAPA
IGPTLSGIILETLNWRWLFYIVLPFAIFSIVFAFIYLKNVSEPTKPKVDVLSILLSTIGFGGIVYGFSSSGEGWDSFQVY
GIILIGLVALLFFVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLFQGALGLTVYATGLIMLPGSL
LNGLLSPVSGKLFDKFGPRALIIPGTLLLASVMWFFTQVTADTSKITFILLHVTMMVSISMIMMPAQTNGLNQLPKRFYP
HGTAILNTLSQVAGAVGVAFFISVMTSGQKKYLDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTS
ES
>Mature_482_residues
MNAQPASVSSQSKEFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQWLSTGYMLVVAVLIPASALL
VQWFTTRQVFIGAMVVFTFGTLVSAIAPGFSILLMGRLLQAAGTGLMMPVLMNTILLLYPPEKRGAAMGSIGLVIMFAPA
IGPTLSGIILETLNWRWLFYIVLPFAIFSIVFAFIYLKNVSEPTKPKVDVLSILLSTIGFGGIVYGFSSSGEGWDSFQVY
GIILIGLVALLFFVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLFQGALGLTVYATGLIMLPGSL
LNGLLSPVSGKLFDKFGPRALIIPGTLLLASVMWFFTQVTADTSKITFILLHVTMMVSISMIMMPAQTNGLNQLPKRFYP
HGTAILNTLSQVAGAVGVAFFISVMTSGQKKYLDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTS
ES

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=25.1833740831296, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789042, Length=459, Percent_Identity=23.7472766884532, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788710, Length=420, Percent_Identity=25, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790195, Length=423, Percent_Identity=26.0047281323877, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788392, Length=394, Percent_Identity=27.9187817258883, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6325455, Length=252, Percent_Identity=25.7936507936508, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52304; Mature: 52304

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQPASVSSQSKEFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
WLSTGYMLVVAVLIPASALLVQWFTTRQVFIGAMVVFTFGTLVSAIAPGFSILLMGRLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAGTGLMMPVLMNTILLLYPPEKRGAAMGSIGLVIMFAPAIGPTLSGIILETLNWRWLFY
HHCCCHHHHHHHHHHEEEECCCHHCCCCCCHHHHHEECCCCCCHHHHHHHHHCCHHHHHH
IVLPFAIFSIVFAFIYLKNVSEPTKPKVDVLSILLSTIGFGGIVYGFSSSGEGWDSFQVY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
GIILIGLVALLFFVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGALGLTVYATGLIMLPGSLLNGLLSPVSGKLFDKFGPRALIIPGTLLLASVMWFFTQVT
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
ADTSKITFILLHVTMMVSISMIMMPAQTNGLNQLPKRFYPHGTAILNTLSQVAGAVGVAF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FISVMTSGQKKYLDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTS
HHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ES
CC
>Mature Secondary Structure
MNAQPASVSSQSKEFDNVKRLPILISMIIGAFFTILNETLLNVAFPQLMIELNVTPSTLQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
WLSTGYMLVVAVLIPASALLVQWFTTRQVFIGAMVVFTFGTLVSAIAPGFSILLMGRLLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AAGTGLMMPVLMNTILLLYPPEKRGAAMGSIGLVIMFAPAIGPTLSGIILETLNWRWLFY
HHCCCHHHHHHHHHHEEEECCCHHCCCCCCHHHHHEECCCCCCHHHHHHHHHCCHHHHHH
IVLPFAIFSIVFAFIYLKNVSEPTKPKVDVLSILLSTIGFGGIVYGFSSSGEGWDSFQVY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
GIILIGLVALLFFVLRQLKLKEPLLDLSAFKYPMFTLTTILLTIMMMTMFSTMTLLPFLF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGALGLTVYATGLIMLPGSLLNGLLSPVSGKLFDKFGPRALIIPGTLLLASVMWFFTQVT
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
ADTSKITFILLHVTMMVSISMIMMPAQTNGLNQLPKRFYPHGTAILNTLSQVAGAVGVAF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
FISVMTSGQKKYLDASSNPTDPAQLTEAMVSGVHNAFTIGFGLALLAVVVALFIKRSQTS
HHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ES
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]