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The map label for this gene is tetB [H]

Identifier: 49183991

GI number: 49183991

Start: 1023741

End: 1025132

Strand: Direct

Name: tetB [H]

Synonym: BAS0970

Alternate gene names: 49183991

Gene position: 1023741-1025132 (Clockwise)

Preceding gene: 49183990

Following gene: 49183992

Centisome position: 19.58

GC content: 38.79

Gene sequence:

>1392_bases
ATGGTAGATCATTCATCACAAAATGCAGATAAATTACTAGGAGTTTTAGTAGTTACGCTAATATTTTCAGTTATGAACGG
TACGATGTTTAATGTAGCTCTTCCTGAAATTGGTAAAGAATTCAACCTTGTTCCTTCAGAGGTTAGTTGGATTATGACGA
GTTATATGGTCGTTTATGCTGTTGGTTCCATCGTAATGGGAAAACTGGCGGATAAATATCGGCTAAAAGATTTGTTAACG
TATGGATTACTTATTTTCGCTCTAGGTTCCTTGCTTGGCTTACTTGCTACTGAGTATTGGGTTATCATTTTAGGGCGTGT
TATTCAAGCAGCGGGAGCCTCTGTTCTTCCTGCGACAGCAATGATTATTCCGGTTAGATACTTTGCGCCAGAGAAAAGGG
GGCGTGCGCTTGGAACTTCAGCAGTTGGTTTAGCGCTTGGGAATGCTTTAGGGCCCGTTGCCGCTGGATTAATTACGAGT
TTTGGAAGCTGGAGACTTATGTTTGTTCTTTCATTACTACCACTTTTAACGCTACCTTTCTTTAGAAAGTATTTAGACAA
TGCAAAAGGGAAGGCGGGTAGTATTGATATTCTTGGCGGAGGTCTATTAGCTGTATCAGTCGCGTTCTTCTTGCTTGCTA
TTACGCAAATGCAAGTGCTGTTATTCCTTAGTGGATTTGCTACGTTAGCTTTCTTCATTTTGCGGATTAGAAAAGCTAAA
GAGCCTTTTATTAAACCGATTCTGTTTAAAAATAAGAACTTTTCGATTGGGTTACTCCTTGCTTTTATGACGACAGCGAT
GAGCTTTAGTATGACATTCATGACGCCGCAATTTCTATCAGCAGTAAATGGATTGACTCCTTCGAATATTGGTTTTGTTT
TAGTTCCAGCTGCAATTGCCTCGGCAATTATGGGAAGAAAGGGAGGAAGGGTAGCTGATACTCGGGGCAATTTTGCACTT
GTTTTTATTGCCTCAGTATTCATATTCCTTGCTTTCTCATTATTATCTACTTTCATTGGGGTCTCGGCTTTTGTCATAGC
ATTGATTTTAATTTTCGGAAATGTAGGACAGACCTTTATGCAGATTTCAATGTCTAATACAATTTCACAAACACTGTCAA
AGGAAGAGACAGGTGTTGGAATGGGATTACTGTCGATGATTAATTTCATATCTGGCGCGATGGCTATGAGTGTAGTAGGG
AAATTGCTTGATAAGGGATCGACTTCACTGAAGCTAAATCCTTTTGTTGCAAATGAAGCTGCAAATATGTATAGTAATAT
CTTCGGCGTGATGTCATTATTGATCTTATTTGTAGTTGTGCTGTATCGTTTTCAATTTGGTACGGGCGTTTCTACGTTAA
ATATGACTAGCAAAGGTAATAAAAATTTATAA

Upstream 100 bases:

>100_bases
AATGATATCCTCATTATAAAAAAATAGTGAGTATATTCATTATGTTGATACTCATTTAAATCTGAATAGATTACCGGAAA
AAGAAAAGGAGGTATGCACG

Downstream 100 bases:

>100_bases
AAGAATACAAGGAGGTTTATGTATGAATGCTCAACCTGCATCTGTTTCTTCGCAGTCAAAGGAGTTTGACAATGTGAAAA
GACTCCCCATTTTAATATCG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVDHSSQNADKLLGVLVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILFVVVLYRFQFGTGVSTLNMTSKGNKNL

Sequences:

>Translated_463_residues
MVDHSSQNADKLLGVLVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILFVVVLYRFQFGTGVSTLNMTSKGNKNL
>Mature_463_residues
MVDHSSQNADKLLGVLVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILFVVVLYRFQFGTGVSTLNMTSKGNKNL

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=313, Percent_Identity=24.6006389776358, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=23.6585365853659, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789042, Length=439, Percent_Identity=23.6902050113895, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788392, Length=407, Percent_Identity=24.8157248157248, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788710, Length=317, Percent_Identity=24.9211356466877, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=139, Percent_Identity=27.3381294964029, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49620; Mature: 49620

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHSSQNADKLLGVLVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHCCCCCCCCHHHHHHHHHHHHHHHH
VGSIVMGKLADKYRLKDLLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHH
SAIMGRKGGRVADTRGNFALVFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFM
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVGKLLDKGSTSLKLNPFVANEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
ANMYSNIFGVMSLLILFVVVLYRFQFGTGVSTLNMTSKGNKNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MVDHSSQNADKLLGVLVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHCCCCCCCCHHHHHHHHHHHHHHHH
VGSIVMGKLADKYRLKDLLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHH
SAIMGRKGGRVADTRGNFALVFIASVFIFLAFSLLSTFIGVSAFVIALILIFGNVGQTFM
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVGKLLDKGSTSLKLNPFVANEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
ANMYSNIFGVMSLLILFVVVLYRFQFGTGVSTLNMTSKGNKNL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377 [H]