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The map label for this gene is tolB

Identifier: 46447484

GI number: 46447484

Start: 2210293

End: 2211702

Strand: Direct

Name: tolB

Synonym: pc1850

Alternate gene names: 46447484

Gene position: 2210293-2211702 (Clockwise)

Preceding gene: 46447483

Following gene: 46447485

Centisome position: 91.54

GC content: 35.46

Gene sequence:

>1410_bases
ATGTATGCATTTTTTTTTAAAACATCTAACCATTGGATACAATTTCTCATCTGCTTTTGTTTATCTTTAGCCCCCTTTTT
TGCTCATTCGATAGACGAAGACCCTATTGTTGTTCGCCTCTCGACAGATTCTTCTCTTGTTCCTCTTTATTTAGCTCCTT
TCACTATAGAACAGACAAATTTTTCTCCATCCTATCTTAAACAATTGGAAGATATCTTAACATTTGATCTTAATCACAAT
GGATCTACTTATTTATCAAAAAGAAATGCCTCCAATGACCAGTTAGCCAATGCAGGTGAGCTAGAAGATTTAGGTATTGC
TCAAACTTGGCAAGCACAAAATATTTTTTATGTGATCAAAGGTATTATCAAAGAAAACTTTCTTCAGGTATTTATATTAA
ATACGAATACACAAAATCTTAAAAAATGTGATCCCGTTATGCTAACCGGTGATTTATCCCAAGATCGCCGATTAATTCAT
CGTATTGCAGACACTATTCATAAAGCCTTATTTGGAGTGGAGGGAGTTGCCTCTACTAAAATTTTGTACACAGTTAAAAC
TCCTCTTGTCGATAATAAACAAAAACTTTCTTCCGAAGTTTGGGAAGCTGATTACGATGGAGAAAATGCACGACAAATTA
CCCAAGAAAAATCTTTTTGTGTGAATCCTACTTACATTCCACCAAAAAAAGGTTTTTTATCAGGCAATTTTCTTTATGTT
TCTTATCAAGCCGGACAATCAAAAATTTATATTGCGAATTTAAAAGATGGCAAAGGACAGCGTTTATTGAAGCTTAAAGG
TAATCAACTGATGCCTACACTTTCTCAACAACGAGATAAAATAGCCTTTATTAGCGACGCAACTGGCAATCCCGATTTAT
TTTTGCAACTTTTCAATCCTGAAACAGGTGTTGTTGGAAAACCGCAGCAAATTTTCTCTGCTAAATTAGCTACCCAAAGT
ACTCCTTCATTTAATCCTGATGGAACCAAAGTAGCTTTTGTATCTGATAAAGATGGTTCTCCAAAAATTTACGTCATTGC
CATTCCAGAACCTGGAACAAGTTTAAAAAATATAAAGGCAACTCTAATTACTAAGCGCAATCGAGAAAGTTCTGCTCCTG
CATGGTCACCAGACGGAACAAAAATTGCTTATTGCTCCCGAACTGATGGGATTAGGCAAATTTGGATTTACGATTTTACA
ACCAACCAGGAAAAACAATTAACGCAAGGACCTATTAACAAGGAAAATCCTTCGTGGGCTCCCAATAGCCTTCATCTTGT
TTATAATTCAGCTGATACAAATGATAGCCAGCTCTATTTGATTAATTTGAATCAAACGGAGGCAACTCGCATAACTTCAG
GAAAAGGAGAAAAACGTTATCCCAATTGGGAGTTACGTTTTGATCGCTAA

Upstream 100 bases:

>100_bases
CAATACACTTTTTTAGTTACGTTAAATAACGATTATTAACCTGGATTACAAGTTTATTCACTAAAAAATCTATTAGATTA
TTAAAAACCCAAGAAATTTT

Downstream 100 bases:

>100_bases
TCGCTTGTTTATCATCTAATTAGATAAGAGATTGAGAATGCTAGTGTTTTTTTTTGCTAATTTGACATGATTTTTGTTAA
ACTATGTTCGACTTTTTTCG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MYAFFFKTSNHWIQFLICFCLSLAPFFAHSIDEDPIVVRLSTDSSLVPLYLAPFTIEQTNFSPSYLKQLEDILTFDLNHN
GSTYLSKRNASNDQLANAGELEDLGIAQTWQAQNIFYVIKGIIKENFLQVFILNTNTQNLKKCDPVMLTGDLSQDRRLIH
RIADTIHKALFGVEGVASTKILYTVKTPLVDNKQKLSSEVWEADYDGENARQITQEKSFCVNPTYIPPKKGFLSGNFLYV
SYQAGQSKIYIANLKDGKGQRLLKLKGNQLMPTLSQQRDKIAFISDATGNPDLFLQLFNPETGVVGKPQQIFSAKLATQS
TPSFNPDGTKVAFVSDKDGSPKIYVIAIPEPGTSLKNIKATLITKRNRESSAPAWSPDGTKIAYCSRTDGIRQIWIYDFT
TNQEKQLTQGPINKENPSWAPNSLHLVYNSADTNDSQLYLINLNQTEATRITSGKGEKRYPNWELRFDR

Sequences:

>Translated_469_residues
MYAFFFKTSNHWIQFLICFCLSLAPFFAHSIDEDPIVVRLSTDSSLVPLYLAPFTIEQTNFSPSYLKQLEDILTFDLNHN
GSTYLSKRNASNDQLANAGELEDLGIAQTWQAQNIFYVIKGIIKENFLQVFILNTNTQNLKKCDPVMLTGDLSQDRRLIH
RIADTIHKALFGVEGVASTKILYTVKTPLVDNKQKLSSEVWEADYDGENARQITQEKSFCVNPTYIPPKKGFLSGNFLYV
SYQAGQSKIYIANLKDGKGQRLLKLKGNQLMPTLSQQRDKIAFISDATGNPDLFLQLFNPETGVVGKPQQIFSAKLATQS
TPSFNPDGTKVAFVSDKDGSPKIYVIAIPEPGTSLKNIKATLITKRNRESSAPAWSPDGTKIAYCSRTDGIRQIWIYDFT
TNQEKQLTQGPINKENPSWAPNSLHLVYNSADTNDSQLYLINLNQTEATRITSGKGEKRYPNWELRFDR
>Mature_469_residues
MYAFFFKTSNHWIQFLICFCLSLAPFFAHSIDEDPIVVRLSTDSSLVPLYLAPFTIEQTNFSPSYLKQLEDILTFDLNHN
GSTYLSKRNASNDQLANAGELEDLGIAQTWQAQNIFYVIKGIIKENFLQVFILNTNTQNLKKCDPVMLTGDLSQDRRLIH
RIADTIHKALFGVEGVASTKILYTVKTPLVDNKQKLSSEVWEADYDGENARQITQEKSFCVNPTYIPPKKGFLSGNFLYV
SYQAGQSKIYIANLKDGKGQRLLKLKGNQLMPTLSQQRDKIAFISDATGNPDLFLQLFNPETGVVGKPQQIFSAKLATQS
TPSFNPDGTKVAFVSDKDGSPKIYVIAIPEPGTSLKNIKATLITKRNRESSAPAWSPDGTKIAYCSRTDGIRQIWIYDFT
TNQEKQLTQGPINKENPSWAPNSLHLVYNSADTNDSQLYLINLNQTEATRITSGKGEKRYPNWELRFDR

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=442, Percent_Identity=23.3031674208145, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_PARUW (Q6MA25)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008849.1
- ProteinModelPortal:   Q6MA25
- SMR:   Q6MA25
- STRING:   Q6MA25
- GeneID:   2781091
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1850
- NMPDR:   fig|264201.1.peg.1850
- eggNOG:   COG0823
- HOGENOM:   HBG415163
- OMA:   RNRESSA
- PhylomeDB:   Q6MA25
- ProtClustDB:   PRK01029
- BioCyc:   CPRO264201:PC1850-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10

Pfam domain/function: PF07676 PD40; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 52829; Mature: 52829

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAFFFKTSNHWIQFLICFCLSLAPFFAHSIDEDPIVVRLSTDSSLVPLYLAPFTIEQTN
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEEECCC
FSPSYLKQLEDILTFDLNHNGSTYLSKRNASNDQLANAGELEDLGIAQTWQAQNIFYVIK
CCHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
GIIKENFLQVFILNTNTQNLKKCDPVMLTGDLSQDRRLIHRIADTIHKALFGVEGVASTK
HHHHCCEEEEEEEECCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILYTVKTPLVDNKQKLSSEVWEADYDGENARQITQEKSFCVNPTYIPPKKGFLSGNFLYV
EEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEE
SYQAGQSKIYIANLKDGKGQRLLKLKGNQLMPTLSQQRDKIAFISDATGNPDLFLQLFNP
EEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCCCCCEEEEEEECC
ETGVVGKPQQIFSAKLATQSTPSFNPDGTKVAFVSDKDGSPKIYVIAIPEPGTSLKNIKA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHEEE
TLITKRNRESSAPAWSPDGTKIAYCSRTDGIRQIWIYDFTTNQEKQLTQGPINKENPSWA
EEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCC
PNSLHLVYNSADTNDSQLYLINLNQTEATRITSGKGEKRYPNWELRFDR
CCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEEEECC
>Mature Secondary Structure
MYAFFFKTSNHWIQFLICFCLSLAPFFAHSIDEDPIVVRLSTDSSLVPLYLAPFTIEQTN
CEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEEECCC
FSPSYLKQLEDILTFDLNHNGSTYLSKRNASNDQLANAGELEDLGIAQTWQAQNIFYVIK
CCHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHH
GIIKENFLQVFILNTNTQNLKKCDPVMLTGDLSQDRRLIHRIADTIHKALFGVEGVASTK
HHHHCCEEEEEEEECCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
ILYTVKTPLVDNKQKLSSEVWEADYDGENARQITQEKSFCVNPTYIPPKKGFLSGNFLYV
EEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEE
SYQAGQSKIYIANLKDGKGQRLLKLKGNQLMPTLSQQRDKIAFISDATGNPDLFLQLFNP
EEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHCCCCCEEEEECCCCCCEEEEEEECC
ETGVVGKPQQIFSAKLATQSTPSFNPDGTKVAFVSDKDGSPKIYVIAIPEPGTSLKNIKA
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHEEE
TLITKRNRESSAPAWSPDGTKIAYCSRTDGIRQIWIYDFTTNQEKQLTQGPINKENPSWA
EEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCCC
PNSLHLVYNSADTNDSQLYLINLNQTEATRITSGKGEKRYPNWELRFDR
CCEEEEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA