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The map label for this gene is ada [H]

Identifier: 46447469

GI number: 46447469

Start: 2193352

End: 2193873

Strand: Direct

Name: ada [H]

Synonym: pc1835

Alternate gene names: 46447469

Gene position: 2193352-2193873 (Clockwise)

Preceding gene: 46447467

Following gene: 46447480

Centisome position: 90.84

GC content: 36.59

Gene sequence:

>522_bases
ATGCATAACAAACATGTAGAGCAGATATTTTTTTCTATCGGTAATTCTAGCTTTGGACAAGTCATTGTTGCTCAAAGCCA
AGTAGGGCTTTGCGCAGTTATTCTAGGGGAAAATCCTGCAGAACTTCAATTACTCCTTCAAGCTCGTTTTCCTCAAGCTC
ATTTAATTCATGATGATTACAAAACAAAAAACATACTTTCTCAAATTATTGAGTTAATGGAAAATCCTCTCAAAAAAATA
GACTTTACTTTGGATGAAAGAGGAACCCTCTTTCAAAGATCTGTCTGGAAAGCCTTAAGAGAAATTCCAATTGGAACAAC
ATCTAGCTATGCAGAAATTGCAAAAAAAATTAGCTCGCCAAAAGCTGTTCGTGCTGTAGGAGGAGCTTGTGCGGCTAATC
CTTTAGCTCTTGTGACACCTTGCCATCGAGTTATTAGAAAAGACGGAAACTTATCTGGGTATCGGTGGGGAATTGAACGC
AAGTTGCAATATCTTCAATTGGAAAGTAAATTAAAAAAATAA

Upstream 100 bases:

>100_bases
AAAGTTAGGAAAGGGTTAAAAGATTTGATGACCTAAACTGGCGAGTCTTAAAGTATAAAATTAGATATGCTTGTTAGAAC
ATAAAAGAGAGGGATAAAAC

Downstream 100 bases:

>100_bases
AAAAGTCGCTTAAAAGAATATCCCTTAAGCGACTAAATTTAGAAGAAAACAATATCTTTTAAAAAGAAAATATAGAGCTT
AAAATACCCTTCTTAGAAAA

Product: putative methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MHNKHVEQIFFSIGNSSFGQVIVAQSQVGLCAVILGENPAELQLLLQARFPQAHLIHDDYKTKNILSQIIELMENPLKKI
DFTLDERGTLFQRSVWKALREIPIGTTSSYAEIAKKISSPKAVRAVGGACAANPLALVTPCHRVIRKDGNLSGYRWGIER
KLQYLQLESKLKK

Sequences:

>Translated_173_residues
MHNKHVEQIFFSIGNSSFGQVIVAQSQVGLCAVILGENPAELQLLLQARFPQAHLIHDDYKTKNILSQIIELMENPLKKI
DFTLDERGTLFQRSVWKALREIPIGTTSSYAEIAKKISSPKAVRAVGGACAANPLALVTPCHRVIRKDGNLSGYRWGIER
KLQYLQLESKLKK
>Mature_173_residues
MHNKHVEQIFFSIGNSSFGQVIVAQSQVGLCAVILGENPAELQLLLQARFPQAHLIHDDYKTKNILSQIIELMENPLKKI
DFTLDERGTLFQRSVWKALREIPIGTTSSYAEIAKKISSPKAVRAVGGACAANPLALVTPCHRVIRKDGNLSGYRWGIER
KLQYLQLESKLKK

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=162, Percent_Identity=44.4444444444444, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1787596, Length=84, Percent_Identity=47.6190476190476, Blast_Score=91, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=125, Percent_Identity=44, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI115533070, Length=121, Percent_Identity=44.6280991735537, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=44.578313253012, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137554, Length=160, Percent_Identity=31.875, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19365; Mature: 19365

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNKHVEQIFFSIGNSSFGQVIVAQSQVGLCAVILGENPAELQLLLQARFPQAHLIHDDY
CCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCHHEECCCH
KTKNILSQIIELMENPLKKIDFTLDERGTLFQRSVWKALREIPIGTTSSYAEIAKKISSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
KAVRAVGGACAANPLALVTPCHRVIRKDGNLSGYRWGIERKLQYLQLESKLKK
HHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNKHVEQIFFSIGNSSFGQVIVAQSQVGLCAVILGENPAELQLLLQARFPQAHLIHDDY
CCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCHHEECCCH
KTKNILSQIIELMENPLKKIDFTLDERGTLFQRSVWKALREIPIGTTSSYAEIAKKISSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
KAVRAVGGACAANPLALVTPCHRVIRKDGNLSGYRWGIERKLQYLQLESKLKK
HHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]