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The map label for this gene is apbE [H]

Identifier: 46447336

GI number: 46447336

Start: 2040243

End: 2041316

Strand: Direct

Name: apbE [H]

Synonym: pc1702

Alternate gene names: 46447336

Gene position: 2040243-2041316 (Clockwise)

Preceding gene: 46447335

Following gene: 46447338

Centisome position: 84.5

GC content: 36.22

Gene sequence:

>1074_bases
GTGAATACAATCCATTTATTTACTTATATTCGCTCCTTGCAAACTTTTACTGCCAAATTTCGCTCTTTATTTATGCTTAT
TTGGATGAGTTTTAGTTTTCTTTTGATGGAAGGTTGTCAAACTTCTCAATCTGAAGTTGTAACCACTTTTCAAGATGTCG
TCATGACAGTACAATACCGTATCCTAGTGGGGAATTCTGTCTCAGAGGTAGACAAAATTCGTATTCAAACGATCATCGAT
TCGACATTTGAAGAAATTGATACCATCTACAATAAATGGAATCCCCAATCTGAAATTTCTCGGTTGAATTCTTTAAAAGC
CTTTCAAACAATTCCCCTTTCTCCTCAATTATTTCAATTATTTAAGCAAATTGATGCACTGGTAATTTTATCCGAAGGGC
TTTTTGATCCAACGATAGAGCCGTTGCAAAAACTTTGGAAAGAAAAACTAAACTCTCAACAGCTTCCCTCTCCAGATGAA
ATAGCTCTCATTAAACCCTCTATTGGGTGGGATAAGATTCATTTTGAAAAAGGAATCTTTTTTAAAGAAAATTCTTTGAC
AGAACTTGACCTAGGAGGAATTGCTAAAGGTTTTGCTATCGATTTGTTAATTTCTCGCTTAAATGAAGCTGGATTTCCCA
ATCTTTATGTAGAATGGGGAGGAGACATTCGGACATCAGGCCGCCATCCTGCAAAAAGACCCTGGAATATTTATATCAGT
CATCTCGATAGTACCGACCCTAAAGATGCGATTGCCTTTTTATCCCTCAACAATCAAGCAATTGCCACGAGCGGAGATTA
TTTCCAATACTGGACAGTCATAAGTAACAACGAATTACAAACCTACTGTCATATTTTTAATCCTTTAACACTCTCCCCAT
TAAAAATTCGTACAGGGTCTATTGCTAGCGCCAGTTTAGTTGCAAACGATTGTTTAACTGCAGATGCACTTGCAAAAGTC
ATGATGTTATTTGATTCTAAAGAAGAAGCAGAACAATGGGTAAAAAAAATGCAATCGCAAATTCCTTCCTTAGCTTATTG
GATGTTAATTCGAGCCTCTTTTAATCCCGAATAA

Upstream 100 bases:

>100_bases
CTTTTATCACACCAGTACCCGGAGGTGTAGGACCAATGACAATCGCTATGCTTTTATATAATACTCTATTAAGCTATTCG
AAATCGCAGAATTTAAACTT

Downstream 100 bases:

>100_bases
AATCTTTAAATTCTGGGTAATGATGTATGTTAAAAAAGAATTATAAACTTCAATTTTCAAAAGCCATTTTCAACTTTTCA
GTAAAAATATTGAGTAAAGA

Product: putative thiamine biosynthesis lipoprotein apbE precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNTIHLFTYIRSLQTFTAKFRSLFMLIWMSFSFLLMEGCQTSQSEVVTTFQDVVMTVQYRILVGNSVSEVDKIRIQTIID
STFEEIDTIYNKWNPQSEISRLNSLKAFQTIPLSPQLFQLFKQIDALVILSEGLFDPTIEPLQKLWKEKLNSQQLPSPDE
IALIKPSIGWDKIHFEKGIFFKENSLTELDLGGIAKGFAIDLLISRLNEAGFPNLYVEWGGDIRTSGRHPAKRPWNIYIS
HLDSTDPKDAIAFLSLNNQAIATSGDYFQYWTVISNNELQTYCHIFNPLTLSPLKIRTGSIASASLVANDCLTADALAKV
MMLFDSKEEAEQWVKKMQSQIPSLAYWMLIRASFNPE

Sequences:

>Translated_357_residues
MNTIHLFTYIRSLQTFTAKFRSLFMLIWMSFSFLLMEGCQTSQSEVVTTFQDVVMTVQYRILVGNSVSEVDKIRIQTIID
STFEEIDTIYNKWNPQSEISRLNSLKAFQTIPLSPQLFQLFKQIDALVILSEGLFDPTIEPLQKLWKEKLNSQQLPSPDE
IALIKPSIGWDKIHFEKGIFFKENSLTELDLGGIAKGFAIDLLISRLNEAGFPNLYVEWGGDIRTSGRHPAKRPWNIYIS
HLDSTDPKDAIAFLSLNNQAIATSGDYFQYWTVISNNELQTYCHIFNPLTLSPLKIRTGSIASASLVANDCLTADALAKV
MMLFDSKEEAEQWVKKMQSQIPSLAYWMLIRASFNPE
>Mature_357_residues
MNTIHLFTYIRSLQTFTAKFRSLFMLIWMSFSFLLMEGCQTSQSEVVTTFQDVVMTVQYRILVGNSVSEVDKIRIQTIID
STFEEIDTIYNKWNPQSEISRLNSLKAFQTIPLSPQLFQLFKQIDALVILSEGLFDPTIEPLQKLWKEKLNSQQLPSPDE
IALIKPSIGWDKIHFEKGIFFKENSLTELDLGGIAKGFAIDLLISRLNEAGFPNLYVEWGGDIRTSGRHPAKRPWNIYIS
HLDSTDPKDAIAFLSLNNQAIATSGDYFQYWTVISNNELQTYCHIFNPLTLSPLKIRTGSIASASLVANDCLTADALAKV
MMLFDSKEEAEQWVKKMQSQIPSLAYWMLIRASFNPE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=287, Percent_Identity=24.0418118466899, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 40719; Mature: 40719

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIHLFTYIRSLQTFTAKFRSLFMLIWMSFSFLLMEGCQTSQSEVVTTFQDVVMTVQYR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
ILVGNSVSEVDKIRIQTIIDSTFEEIDTIYNKWNPQSEISRLNSLKAFQTIPLSPQLFQL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
FKQIDALVILSEGLFDPTIEPLQKLWKEKLNSQQLPSPDEIALIKPSIGWDKIHFEKGIF
HHHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCEE
FKENSLTELDLGGIAKGFAIDLLISRLNEAGFPNLYVEWGGDIRTSGRHPAKRPWNIYIS
EECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEE
HLDSTDPKDAIAFLSLNNQAIATSGDYFQYWTVISNNELQTYCHIFNPLTLSPLKIRTGS
ECCCCCCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCEEHHHHCCCCCCCCEEEECCC
IASASLVANDCLTADALAKVMMLFDSKEEAEQWVKKMQSQIPSLAYWMLIRASFNPE
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNTIHLFTYIRSLQTFTAKFRSLFMLIWMSFSFLLMEGCQTSQSEVVTTFQDVVMTVQYR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEE
ILVGNSVSEVDKIRIQTIIDSTFEEIDTIYNKWNPQSEISRLNSLKAFQTIPLSPQLFQL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
FKQIDALVILSEGLFDPTIEPLQKLWKEKLNSQQLPSPDEIALIKPSIGWDKIHFEKGIF
HHHHHHEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEECCCEE
FKENSLTELDLGGIAKGFAIDLLISRLNEAGFPNLYVEWGGDIRTSGRHPAKRPWNIYIS
EECCCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEEEE
HLDSTDPKDAIAFLSLNNQAIATSGDYFQYWTVISNNELQTYCHIFNPLTLSPLKIRTGS
ECCCCCCCCEEEEEEECCCEEEECCCCEEEEEEEECCCCEEHHHHCCCCCCCCEEEECCC
IASASLVANDCLTADALAKVMMLFDSKEEAEQWVKKMQSQIPSLAYWMLIRASFNPE
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]