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The map label for this gene is rsmE [H]

Identifier: 46447268

GI number: 46447268

Start: 1952953

End: 1953675

Strand: Direct

Name: rsmE [H]

Synonym: pc1634

Alternate gene names: 46447268

Gene position: 1952953-1953675 (Clockwise)

Preceding gene: 46447267

Following gene: 46447271

Centisome position: 80.89

GC content: 39.56

Gene sequence:

>723_bases
ATGCCAGCTGAGCGTTTTTTTACCTCTGAGGAAATATCTTTGCATCAACATGTGCTATTAAAAGATAATGAATTTCACCA
TCTTGCGCACGTGATGAGGGCAAAAAAAGGTGAGCGTGTTGAACTCGTGAATGGTAAAAGCATTTTAGCCCAGGCTACTG
TTTTAGACATCCATAAAGGCCAAGCAGTCTTACTAATAGATCACATAAAAACTGAACGAATGGATTCTTCACGCTTAATA
TTAGCTCAAGCTTATGTCAAACCTGATCGCTTAGCCTTTATTTTGGAGAAAGGAACAGAGCTAGGCGTCGATGAGTTTTG
GCTTTTTCCTGGTCAACTAAGTTCCCAAAAAGAAATTTATCCTCACCAACAAGAACGCGCCCAATCTCTAATCATCGCCG
CTATGAAACAATGTGGTCGTTTAACTCTTCCTAAAATTCTTTTTAAGCCGGCTTTAAATCAATGGGATAGTATTTTTTCA
CAGGCTTTTTTTGGCGATTTAGATCCTCATGCCCCCTTTCTTGCTGCTGCAAATGAAGGGTCCTCTTATCCTATTATTTT
TTTTACAGGCCCTGAAAGTGGTTGGAGTGATGCAGAACGGCAGCAGTTAAAAAAAATGGGTGTGCAAGGAGTCAGGTTAC
ATACTAACATTCTGCGCACTGATACAGCTTCAATTGCTGCTTTGAGTATTATTCAACAATGGAAATTCATTCCGTTTAAG
TAA

Upstream 100 bases:

>100_bases
CTTTATTTAAATTCGAATCAAAAATTGGAATTAGAAAGCTCTTTGAAAGATCAAGAAATTCGAATTAAGATTCCGGCCTT
ATCTCAAGGAGTTCCAACTA

Downstream 100 bases:

>100_bases
ACCTAATAGTTCTTTTCTTTTTTTTGCATGATTAGCTTGGATAATGATTTCTCTTTTGAGCAAAGCTCCACCATAGACAT
ATCTATATTTATTTGGTAAT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPAERFFTSEEISLHQHVLLKDNEFHHLAHVMRAKKGERVELVNGKSILAQATVLDIHKGQAVLLIDHIKTERMDSSRLI
LAQAYVKPDRLAFILEKGTELGVDEFWLFPGQLSSQKEIYPHQQERAQSLIIAAMKQCGRLTLPKILFKPALNQWDSIFS
QAFFGDLDPHAPFLAAANEGSSYPIIFFTGPESGWSDAERQQLKKMGVQGVRLHTNILRTDTASIAALSIIQQWKFIPFK

Sequences:

>Translated_240_residues
MPAERFFTSEEISLHQHVLLKDNEFHHLAHVMRAKKGERVELVNGKSILAQATVLDIHKGQAVLLIDHIKTERMDSSRLI
LAQAYVKPDRLAFILEKGTELGVDEFWLFPGQLSSQKEIYPHQQERAQSLIIAAMKQCGRLTLPKILFKPALNQWDSIFS
QAFFGDLDPHAPFLAAANEGSSYPIIFFTGPESGWSDAERQQLKKMGVQGVRLHTNILRTDTASIAALSIIQQWKFIPFK
>Mature_239_residues
PAERFFTSEEISLHQHVLLKDNEFHHLAHVMRAKKGERVELVNGKSILAQATVLDIHKGQAVLLIDHIKTERMDSSRLIL
AQAYVKPDRLAFILEKGTELGVDEFWLFPGQLSSQKEIYPHQQERAQSLIIAAMKQCGRLTLPKILFKPALNQWDSIFSQ
AFFGDLDPHAPFLAAANEGSSYPIIFFTGPESGWSDAERQQLKKMGVQGVRLHTNILRTDTASIAALSIIQQWKFIPFK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=237, Percent_Identity=23.6286919831224, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27185; Mature: 27054

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAERFFTSEEISLHQHVLLKDNEFHHLAHVMRAKKGERVELVNGKSILAQATVLDIHKG
CCHHHCCCCCHHHHHEEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEECCC
QAVLLIDHIKTERMDSSRLILAQAYVKPDRLAFILEKGTELGVDEFWLFPGQLSSQKEIY
CEEEEEECHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHEEECCCCCCCCCCCC
PHQQERAQSLIIAAMKQCGRLTLPKILFKPALNQWDSIFSQAFFGDLDPHAPFLAAANEG
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SSYPIIFFTGPESGWSDAERQQLKKMGVQGVRLHTNILRTDTASIAALSIIQQWKFIPFK
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PAERFFTSEEISLHQHVLLKDNEFHHLAHVMRAKKGERVELVNGKSILAQATVLDIHKG
CHHHCCCCCHHHHHEEEEECCCCHHHHHHHHHHCCCCEEEEECCCHHHHHEEEEEECCC
QAVLLIDHIKTERMDSSRLILAQAYVKPDRLAFILEKGTELGVDEFWLFPGQLSSQKEIY
CEEEEEECHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCHHEEECCCCCCCCCCCC
PHQQERAQSLIIAAMKQCGRLTLPKILFKPALNQWDSIFSQAFFGDLDPHAPFLAAANEG
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
SSYPIIFFTGPESGWSDAERQQLKKMGVQGVRLHTNILRTDTASIAALSIIQQWKFIPFK
CCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]