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The map label for this gene is trmB

Identifier: 46446919

GI number: 46446919

Start: 1540860

End: 1541537

Strand: Direct

Name: trmB

Synonym: pc1285

Alternate gene names: 46446919

Gene position: 1540860-1541537 (Clockwise)

Preceding gene: 46446918

Following gene: 46446920

Centisome position: 63.82

GC content: 35.84

Gene sequence:

>678_bases
ATGAAGCCAAAAGATTTAAAATCACCATTTAAATGGGAACATCGTCAAATCGTTATTCAAGATCGAGTTTGGTATGTTCC
GGATCAGCACTTATCGGACATCCCTTTTATCTTTCCAGGTTGGACGCATGACCAAACATTTGCTCAAGACAAACCTATCT
ACTTAGAGTATTGCAGCGGAAATGGTGCATGGATTGCTTCTAAAGCCATTGAACAGTCGCAATATAACTGGGTTGGTATT
GAAAAAAAATTTGATCGAACGCGTAAAATTTGGTCCAAGATTAAGAAATTTGAACTTGATAATCTCTTAACAATTTGTGG
AGAAGGGTTTCATGTTACCCGAGATTTTTTTGCTAATGAAAGTGTGGATGCCGTTTTTATTAATTTTCCAGATCCATGGC
CCAAAAAAAGACATGCTAAACACCGCATTGTTCAACCAGCTTTTGTCAAAGAAATTCATCGTATCTTAAAAAGTGATAAA
CTCTTTACTCTAGTCACAGACGATGCTGCATATTCAAAAATCATGATTGATGTCATTTATCAAACCGGCGGTTTCATATC
ACTCTTTGAAGCTCCTTATTATACGACTTCTTATCCAGGTTATGGATCTTCTTACTTTGAAGAGCTTTGGAGAGAAAAAG
GAAAATCGATTCATTACCACGTTTTTCGAAAAATTTAA

Upstream 100 bases:

>100_bases
CCCATGTGAAATTGTGAAAATAAATAGTCAATCCTTCGGTTGGTTTGAAATTGCCTTTTCTATATAATTATTTCATTCAA
TTGCAAAATTATCAGTTTAT

Downstream 100 bases:

>100_bases
CCCTAACAGATTCTCATGCAAGTCTCAAACAATGATTTTGAAGAACAGCCTGATCCATGTGAATTACAATGGAATCGCCC
TTTTTATACTTTAAAAAATG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKPKDLKSPFKWEHRQIVIQDRVWYVPDQHLSDIPFIFPGWTHDQTFAQDKPIYLEYCSGNGAWIASKAIEQSQYNWVGI
EKKFDRTRKIWSKIKKFELDNLLTICGEGFHVTRDFFANESVDAVFINFPDPWPKKRHAKHRIVQPAFVKEIHRILKSDK
LFTLVTDDAAYSKIMIDVIYQTGGFISLFEAPYYTTSYPGYGSSYFEELWREKGKSIHYHVFRKI

Sequences:

>Translated_225_residues
MKPKDLKSPFKWEHRQIVIQDRVWYVPDQHLSDIPFIFPGWTHDQTFAQDKPIYLEYCSGNGAWIASKAIEQSQYNWVGI
EKKFDRTRKIWSKIKKFELDNLLTICGEGFHVTRDFFANESVDAVFINFPDPWPKKRHAKHRIVQPAFVKEIHRILKSDK
LFTLVTDDAAYSKIMIDVIYQTGGFISLFEAPYYTTSYPGYGSSYFEELWREKGKSIHYHVFRKI
>Mature_225_residues
MKPKDLKSPFKWEHRQIVIQDRVWYVPDQHLSDIPFIFPGWTHDQTFAQDKPIYLEYCSGNGAWIASKAIEQSQYNWVGI
EKKFDRTRKIWSKIKKFELDNLLTICGEGFHVTRDFFANESVDAVFINFPDPWPKKRHAKHRIVQPAFVKEIHRILKSDK
LFTLVTDDAAYSKIMIDVIYQTGGFISLFEAPYYTTSYPGYGSSYFEELWREKGKSIHYHVFRKI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=179, Percent_Identity=26.2569832402235, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_PARUW (Q6MBP0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008284.1
- ProteinModelPortal:   Q6MBP0
- SMR:   Q6MBP0
- STRING:   Q6MBP0
- GeneID:   2780395
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1285
- NMPDR:   fig|264201.1.peg.1285
- eggNOG:   COG0220
- HOGENOM:   HBG413880
- OMA:   NLRIVCG
- PhylomeDB:   Q6MBP0
- ProtClustDB:   PRK00121
- BioCyc:   CPRO264201:PC1285-MONOMER
- BioCyc:   PCHL-E25-01:PCHL-E25-01-001285-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26668; Mature: 26668

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 81-81 BINDING 108-108 BINDING 131-131 BINDING 135-135 BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKDLKSPFKWEHRQIVIQDRVWYVPDQHLSDIPFIFPGWTHDQTFAQDKPIYLEYCSG
CCCCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECC
NGAWIASKAIEQSQYNWVGIEKKFDRTRKIWSKIKKFELDNLLTICGEGFHVTRDFFANE
CCCEEEHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEHHHHCCC
SVDAVFINFPDPWPKKRHAKHRIVQPAFVKEIHRILKSDKLFTLVTDDAAYSKIMIDVIY
CCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
QTGGFISLFEAPYYTTSYPGYGSSYFEELWREKGKSIHYHVFRKI
HCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure
MKPKDLKSPFKWEHRQIVIQDRVWYVPDQHLSDIPFIFPGWTHDQTFAQDKPIYLEYCSG
CCCCCCCCCCCCCCEEEEEECCEEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEEEECC
NGAWIASKAIEQSQYNWVGIEKKFDRTRKIWSKIKKFELDNLLTICGEGFHVTRDFFANE
CCCEEEHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCEEEHHHHCCC
SVDAVFINFPDPWPKKRHAKHRIVQPAFVKEIHRILKSDKLFTLVTDDAAYSKIMIDVIY
CCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
QTGGFISLFEAPYYTTSYPGYGSSYFEELWREKGKSIHYHVFRKI
HCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA