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The map label for this gene is rimO

Identifier: 46446860

GI number: 46446860

Start: 1460323

End: 1461750

Strand: Direct

Name: rimO

Synonym: pc1226

Alternate gene names: 46446860

Gene position: 1460323-1461750 (Clockwise)

Preceding gene: 46446859

Following gene: 46446861

Centisome position: 60.48

GC content: 37.11

Gene sequence:

>1428_bases
ATGTTGCCTATTTTAAAAAACCAAGGTGTCAAAAAAGATCAAAATCACATTGCATTGAAAAATGATTCTTGCGAATCCTC
TTCCCCTTGTTTTGATCATGAAGGAAATAAAATTAACTTTATTAGTTTGGGTTGTCCTCGCAATCTTGTCGATAGTGAAG
TCATGTTAGGAATTTTATTAAAAGCAGGTTATGAAGTAGCCCCCACTCTCGAAGAAGCTGATTATTTAGTGATTAATACT
TGTGGTTTTTTAGAGGCTTCTCGTCAAGAATCTATGGATACCGTTGAAGAGGTCCTCTCTCAACGAAAAAAAACAGCAAA
GTTAATTGTTACTGGATGTATGGTTCAAACACATAGTGACGCTTTAAAAACCACATTTCCCAGTATTGATTACTTATTAG
GATCAGGAGATGTAGAAGGCATTTTAAAGGCTGTTCAATCCACTCAAAAAGGGCAGATTATTTCTTCAGCAAGAAGCTAC
TTAGAGGCTGGTGAAGTTCCTCGCCGTCTTTCTACTCCTAAACACTACGCTTATTTAAAAATTGCTGAAGGCTGTCGTAA
ACGTTGTGCCTATTGCGTGATTCCCACAATTAAAGGCCCTTTAAAAAGTAAAGGCAAAGAACAAATTCTCAAAGAGTTCA
ATCTCCTTCTTAGTCAAGGTGTTAAAGAGGTCATTTTAATTGCACAAGATTTAGGGGATTACGGAAAAGATCAAGGGGCT
AAAAAATTGACAGCTTTGCTAAACCTTTTGCAATCAATGCTAGAAATCAAACAAGCTTTTTGGCTTCGCCTCCTTTATTT
GTACCCTGATGAAATTACTGATGAGTTAATTGCCTTAATGAAGAGTGATTCTCGCATTTGTCCTTACCTTGACATGCCTA
TTCAACATGTAAACAATCAAATCCTTAAATCCATGAGGCGAGCCACTTCAAAAGAAGATATTATCGAAATTATCACTAAG
TTACGCCGAGAAATTCCAAACGTCGCCATTCGTACCAGTTTAATTGTTGGCTTTCCAGGGGAAACAGAAGAACAATTTCA
AGAACTCATTCAATTTGTCCAGGACTATCCTCTTGAAAACGTTGGAATCTTCAAATTTTCACGAGAACCTGGTTCCCACG
CTTACGATTTACCTAATCAAATTTCTGATGAAATGAAAGAAGATCGTTATCATCGCTTGATGCAAGTTCAGAAGAAAGTT
GTGAAAAAAAATCTCAAAAAAATGATCGGTAAAAAAATCGCAGTTGTAGTGGAAGGTTATCACCCAGAAACGGAACTTCT
CATGATTGGAAGGCATACCGGTCAATGCCCAGATATTGATGGACAAGTCCTGATCAATGATGGACGGAAAGTAAAAGCTT
TTGGAGAAATTTACACGGTTGAAATCACGGATGTTGCAGACTATGATTTAGTAGGACATGTCATCTAA

Upstream 100 bases:

>100_bases
AATAGTTAAAAGAATAAATTGATTTCCAAAATTTATTTCAAAACGTCATTAAATCCGCGCTTGTTCTTGATCAAAAGTTT
ATAAGGGAGTCATTCGTTCT

Downstream 100 bases:

>100_bases
CAATTTAAACTTTAAATTATAAGCTTAGACAAGTTTTGAGAGAAATCCTCACTTATTGTATAAACAATTTGAAAATGAGG
AGTCCCATATGAAATTTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLPILKNQGVKKDQNHIALKNDSCESSSPCFDHEGNKINFISLGCPRNLVDSEVMLGILLKAGYEVAPTLEEADYLVINT
CGFLEASRQESMDTVEEVLSQRKKTAKLIVTGCMVQTHSDALKTTFPSIDYLLGSGDVEGILKAVQSTQKGQIISSARSY
LEAGEVPRRLSTPKHYAYLKIAEGCRKRCAYCVIPTIKGPLKSKGKEQILKEFNLLLSQGVKEVILIAQDLGDYGKDQGA
KKLTALLNLLQSMLEIKQAFWLRLLYLYPDEITDELIALMKSDSRICPYLDMPIQHVNNQILKSMRRATSKEDIIEIITK
LRREIPNVAIRTSLIVGFPGETEEQFQELIQFVQDYPLENVGIFKFSREPGSHAYDLPNQISDEMKEDRYHRLMQVQKKV
VKKNLKKMIGKKIAVVVEGYHPETELLMIGRHTGQCPDIDGQVLINDGRKVKAFGEIYTVEITDVADYDLVGHVI

Sequences:

>Translated_475_residues
MLPILKNQGVKKDQNHIALKNDSCESSSPCFDHEGNKINFISLGCPRNLVDSEVMLGILLKAGYEVAPTLEEADYLVINT
CGFLEASRQESMDTVEEVLSQRKKTAKLIVTGCMVQTHSDALKTTFPSIDYLLGSGDVEGILKAVQSTQKGQIISSARSY
LEAGEVPRRLSTPKHYAYLKIAEGCRKRCAYCVIPTIKGPLKSKGKEQILKEFNLLLSQGVKEVILIAQDLGDYGKDQGA
KKLTALLNLLQSMLEIKQAFWLRLLYLYPDEITDELIALMKSDSRICPYLDMPIQHVNNQILKSMRRATSKEDIIEIITK
LRREIPNVAIRTSLIVGFPGETEEQFQELIQFVQDYPLENVGIFKFSREPGSHAYDLPNQISDEMKEDRYHRLMQVQKKV
VKKNLKKMIGKKIAVVVEGYHPETELLMIGRHTGQCPDIDGQVLINDGRKVKAFGEIYTVEITDVADYDLVGHVI
>Mature_475_residues
MLPILKNQGVKKDQNHIALKNDSCESSSPCFDHEGNKINFISLGCPRNLVDSEVMLGILLKAGYEVAPTLEEADYLVINT
CGFLEASRQESMDTVEEVLSQRKKTAKLIVTGCMVQTHSDALKTTFPSIDYLLGSGDVEGILKAVQSTQKGQIISSARSY
LEAGEVPRRLSTPKHYAYLKIAEGCRKRCAYCVIPTIKGPLKSKGKEQILKEFNLLLSQGVKEVILIAQDLGDYGKDQGA
KKLTALLNLLQSMLEIKQAFWLRLLYLYPDEITDELIALMKSDSRICPYLDMPIQHVNNQILKSMRRATSKEDIIEIITK
LRREIPNVAIRTSLIVGFPGETEEQFQELIQFVQDYPLENVGIFKFSREPGSHAYDLPNQISDEMKEDRYHRLMQVQKKV
VKKNLKKMIGKKIAVVVEGYHPETELLMIGRHTGQCPDIDGQVLINDGRKVKAFGEIYTVEITDVADYDLVGHVI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=25.1046025104602, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=25.1046025104602, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI93277076, Length=375, Percent_Identity=26.9333333333333, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=37.4439461883408, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=27.1910112359551, Blast_Score=172, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=21.5644820295983, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71996771, Length=203, Percent_Identity=29.064039408867, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356207, Length=461, Percent_Identity=26.0303687635575, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI19922432, Length=374, Percent_Identity=27.807486631016, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PARUW (Q6MBU9)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008225.1
- ProteinModelPortal:   Q6MBU9
- SMR:   Q6MBU9
- STRING:   Q6MBU9
- GeneID:   2779564
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1226
- NMPDR:   fig|264201.1.peg.1226
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   LYLYPDE
- PhylomeDB:   Q6MBU9
- ProtClustDB:   CLSK401459
- BioCyc:   CPRO264201:PC1226-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53463; Mature: 53463

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPILKNQGVKKDQNHIALKNDSCESSSPCFDHEGNKINFISLGCPRNLVDSEVMLGILL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
KAGYEVAPTLEEADYLVINTCGFLEASRQESMDTVEEVLSQRKKTAKLIVTGCMVQTHSD
HCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALKTTFPSIDYLLGSGDVEGILKAVQSTQKGQIISSARSYLEAGEVPRRLSTPKHYAYLK
HHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCHHEEHH
IAEGCRKRCAYCVIPTIKGPLKSKGKEQILKEFNLLLSQGVKEVILIAQDLGDYGKDQGA
HHHHHHHHCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
KKLTALLNLLQSMLEIKQAFWLRLLYLYPDEITDELIALMKSDSRICPYLDMPIQHVNNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ILKSMRRATSKEDIIEIITKLRREIPNVAIRTSLIVGFPGETEEQFQELIQFVQDYPLEN
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
VGIFKFSREPGSHAYDLPNQISDEMKEDRYHRLMQVQKKVVKKNLKKMIGKKIAVVVEGY
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
HPETELLMIGRHTGQCPDIDGQVLINDGRKVKAFGEIYTVEITDVADYDLVGHVI
CCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEECCEEEEEEECCCCCCHHCCCC
>Mature Secondary Structure
MLPILKNQGVKKDQNHIALKNDSCESSSPCFDHEGNKINFISLGCPRNLVDSEVMLGILL
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHH
KAGYEVAPTLEEADYLVINTCGFLEASRQESMDTVEEVLSQRKKTAKLIVTGCMVQTHSD
HCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALKTTFPSIDYLLGSGDVEGILKAVQSTQKGQIISSARSYLEAGEVPRRLSTPKHYAYLK
HHHHHCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHCCCCCHHEEHH
IAEGCRKRCAYCVIPTIKGPLKSKGKEQILKEFNLLLSQGVKEVILIAQDLGDYGKDQGA
HHHHHHHHCCEEEECCCCCCHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
KKLTALLNLLQSMLEIKQAFWLRLLYLYPDEITDELIALMKSDSRICPYLDMPIQHVNNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
ILKSMRRATSKEDIIEIITKLRREIPNVAIRTSLIVGFPGETEEQFQELIQFVQDYPLEN
HHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCC
VGIFKFSREPGSHAYDLPNQISDEMKEDRYHRLMQVQKKVVKKNLKKMIGKKIAVVVEGY
CEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
HPETELLMIGRHTGQCPDIDGQVLINDGRKVKAFGEIYTVEITDVADYDLVGHVI
CCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEECCEEEEEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA