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The map label for this gene is priA [H]

Identifier: 46446859

GI number: 46446859

Start: 1457750

End: 1459987

Strand: Direct

Name: priA [H]

Synonym: pc1225

Alternate gene names: 46446859

Gene position: 1457750-1459987 (Clockwise)

Preceding gene: 46446858

Following gene: 46446860

Centisome position: 60.38

GC content: 37.49

Gene sequence:

>2238_bases
ATGAAAACTTTTGCCGCTATTGCTTCGGTTATTTTAGATATATCGATTGACAAAACATTAGATTATGGAATTGTTCCTTC
GCAATTAGAAGCAGCTCAAAAAGGAAGTCGCGTAGAAGTTCCTGTTCGAGGACACTCAAGGACAGGCTATATTGTGGAAA
TTAAATCAGATTCCAACTTTAAAAATATCAAACCAATTACACGTTTATTATCCGAGACTCCTCTTGTTCCCGAAGAACTA
TTTAAGCTCGCTTTATGGGTAGCAAAATATTATTGTGCTCCGTTAAGAGATATTTTCCGTATTATTTTACCCCCCAGTGT
ACGCAAAGGAATGAAAGAAAAAGAACAACTTTTTGTTGTACGGGGCAAATCTAAAGAAATCCTTAGACAGGTTTGTGTTG
ATATTAGAGAGAAAAAGCCAGCGCAGGCTTCTGTATTGGAAGTTATGTTGCAAGTGAAAAATGGCATTTTACTTTCTAAG
CTTCTGGAAGAAAGCAAAAGTTCTCGAAGTAGTGTTTTATCTTTAGCCAAACAACAACTTCTTATTGTCGAAACAATCAA
AATTGACCGTTCTCCGCTTGTGGATGAAGACTACTTTATGACTAAACCCAAAATATTGAATGAAGATCAAGCGGTAGCTT
TAAATAAGATCGATGCCTCTTTAAATGCAAAAATCTTTCAGACCCATTTAATCCACGGTATAACAGGAAGTGGAAAAACA
GAGATATATCTCCAAGCAATAGAAAAAGCTCTGAAGTTAGAAAAAGGAACGATCATGCTCGTTCCTGAAATTTCTTTAAC
AGCACAAACGATTCAACGTTTTAGAAGTCGTTTTGCAGAAAAAATTGCCATTTTACATCATCGATTAAGCGAAGGTGAAA
GACGAGATGAGTGGCATAAAATTCGTGAAGGGCGAGCTAAAATTATTGTTGGTGCTCGCTCAGCCATTTTCAGCCCAGTA
GTTAATTTAGGATTAATTATTGTTGATGAGGAACATGAGCAATCTTATAAGCAAAATGAAATGTCGCCTTGCTATCAAGC
TAGAGATGTTGCCGTGATGCGTGGGAAACTCGCTCAAGCAACTGTTGTGTTAGGAAGTGCAACGCCAAGTCTAGAAAGCT
ATTATAATGCTCTCAATGGAAAATATGCCTTGAGTGTTTTACACAAACGAGCAGATGTAGCTACCCTTCCAGACGTGACT
ATTGTCGATATGAGAAAAGAATTTGAACGAGCAAAAACGTTGACAAGTTTTTCTGAAGTTTTATTGTCTGGAATTGAAAG
AAGGCAAAAACAAGGTGAACAAGTCATTTTATTTCTCAATCGGAGAGGCTACCATACAACGCTTCTATGTCAAGATTGTT
CTCATATTGTTAAATGCACTCATTGCGAAATTCCTTTAACATTTCATTTAGGAGACAATCATCTTTCCTGCCATTTATGT
GGTTATCAAATTTCTCCACCCCCTAAAGAATGCCCTTCCTGTCGAGGAACCAAGCACCTTAAATTTCGAGGAGCTGGAAC
AGAGCATGTTGAAAAAGCCCTTCATGCTATTTTTCCTCAAATACGAACAATGCGAATTGATGCTGATACTACGAAACACA
AAGGAAGCCATCAAAAATTACTCCGTGATTTTGGAACTGGAAAAGCGGATGTATTGATTGGTACACAAATGATCGCAAAA
GGATTACATTTTCCAGAAGTCACCCTTGTTGGCGTTCTTAATAGTGATGCAGGACTTAATATTCCCGATTTCCGAGCTTC
TGAAACAATTTTTCAGCTAATTACACAAGTGGCAGGTCGATCAGGTCGTGGTGTGACAAAAGGGGAAGTCATTATTCAAA
CTTCCATGCCTGAAAATTCTACTATTCAGCATGCTGCCAAGCAAGACTACGCAGGATTTTACGAAGAAGAAATTGCTGTG
CGTGAAATGTTCCACTATCCTCCTTTTTCCCATCTCGCCAAATTAACCTTTAGTGGAAAGCAACCCGATCAAACATATGA
AATTGCTCAGAAGTTTCGAGAATTACTCATTTTTCAACTTCCCCAACACTACGAATTTAATCCTGTTATTCCCTGTGGAT
ATGCAAAAATCAAAGATCTTTACCGATATCAATTTTTGCTACGAGGCCCCTCTATGATTCCTCTCAATTTAGTTCTAGAA
AATTTAATGCAAAAATTTTCCATCCCTAAGGAAATAAAGTTGTTCGTCGATATTAATCCATCTTCAACTTTTTTTTGA

Upstream 100 bases:

>100_bases
TAGATCTTTACCTTAAAGAACCTCAGCTAAAATCTCTCTTGTGGAGAGCTATATGTAATGAATTAACACCTCTCACGAAA
ATCTCAACAACTAGCTAAAA

Downstream 100 bases:

>100_bases
AAGCTGTCATGCTTACTTTTGATTAGCTTGACTAAATAATAAATTATGCCCCTCATTACTTTTTTTAGACTATATTATTA
AAAAATTTTTTACTATTTTA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 745; Mature: 745

Protein sequence:

>745_residues
MKTFAAIASVILDISIDKTLDYGIVPSQLEAAQKGSRVEVPVRGHSRTGYIVEIKSDSNFKNIKPITRLLSETPLVPEEL
FKLALWVAKYYCAPLRDIFRIILPPSVRKGMKEKEQLFVVRGKSKEILRQVCVDIREKKPAQASVLEVMLQVKNGILLSK
LLEESKSSRSSVLSLAKQQLLIVETIKIDRSPLVDEDYFMTKPKILNEDQAVALNKIDASLNAKIFQTHLIHGITGSGKT
EIYLQAIEKALKLEKGTIMLVPEISLTAQTIQRFRSRFAEKIAILHHRLSEGERRDEWHKIREGRAKIIVGARSAIFSPV
VNLGLIIVDEEHEQSYKQNEMSPCYQARDVAVMRGKLAQATVVLGSATPSLESYYNALNGKYALSVLHKRADVATLPDVT
IVDMRKEFERAKTLTSFSEVLLSGIERRQKQGEQVILFLNRRGYHTTLLCQDCSHIVKCTHCEIPLTFHLGDNHLSCHLC
GYQISPPPKECPSCRGTKHLKFRGAGTEHVEKALHAIFPQIRTMRIDADTTKHKGSHQKLLRDFGTGKADVLIGTQMIAK
GLHFPEVTLVGVLNSDAGLNIPDFRASETIFQLITQVAGRSGRGVTKGEVIIQTSMPENSTIQHAAKQDYAGFYEEEIAV
REMFHYPPFSHLAKLTFSGKQPDQTYEIAQKFRELLIFQLPQHYEFNPVIPCGYAKIKDLYRYQFLLRGPSMIPLNLVLE
NLMQKFSIPKEIKLFVDINPSSTFF

Sequences:

>Translated_745_residues
MKTFAAIASVILDISIDKTLDYGIVPSQLEAAQKGSRVEVPVRGHSRTGYIVEIKSDSNFKNIKPITRLLSETPLVPEEL
FKLALWVAKYYCAPLRDIFRIILPPSVRKGMKEKEQLFVVRGKSKEILRQVCVDIREKKPAQASVLEVMLQVKNGILLSK
LLEESKSSRSSVLSLAKQQLLIVETIKIDRSPLVDEDYFMTKPKILNEDQAVALNKIDASLNAKIFQTHLIHGITGSGKT
EIYLQAIEKALKLEKGTIMLVPEISLTAQTIQRFRSRFAEKIAILHHRLSEGERRDEWHKIREGRAKIIVGARSAIFSPV
VNLGLIIVDEEHEQSYKQNEMSPCYQARDVAVMRGKLAQATVVLGSATPSLESYYNALNGKYALSVLHKRADVATLPDVT
IVDMRKEFERAKTLTSFSEVLLSGIERRQKQGEQVILFLNRRGYHTTLLCQDCSHIVKCTHCEIPLTFHLGDNHLSCHLC
GYQISPPPKECPSCRGTKHLKFRGAGTEHVEKALHAIFPQIRTMRIDADTTKHKGSHQKLLRDFGTGKADVLIGTQMIAK
GLHFPEVTLVGVLNSDAGLNIPDFRASETIFQLITQVAGRSGRGVTKGEVIIQTSMPENSTIQHAAKQDYAGFYEEEIAV
REMFHYPPFSHLAKLTFSGKQPDQTYEIAQKFRELLIFQLPQHYEFNPVIPCGYAKIKDLYRYQFLLRGPSMIPLNLVLE
NLMQKFSIPKEIKLFVDINPSSTFF
>Mature_745_residues
MKTFAAIASVILDISIDKTLDYGIVPSQLEAAQKGSRVEVPVRGHSRTGYIVEIKSDSNFKNIKPITRLLSETPLVPEEL
FKLALWVAKYYCAPLRDIFRIILPPSVRKGMKEKEQLFVVRGKSKEILRQVCVDIREKKPAQASVLEVMLQVKNGILLSK
LLEESKSSRSSVLSLAKQQLLIVETIKIDRSPLVDEDYFMTKPKILNEDQAVALNKIDASLNAKIFQTHLIHGITGSGKT
EIYLQAIEKALKLEKGTIMLVPEISLTAQTIQRFRSRFAEKIAILHHRLSEGERRDEWHKIREGRAKIIVGARSAIFSPV
VNLGLIIVDEEHEQSYKQNEMSPCYQARDVAVMRGKLAQATVVLGSATPSLESYYNALNGKYALSVLHKRADVATLPDVT
IVDMRKEFERAKTLTSFSEVLLSGIERRQKQGEQVILFLNRRGYHTTLLCQDCSHIVKCTHCEIPLTFHLGDNHLSCHLC
GYQISPPPKECPSCRGTKHLKFRGAGTEHVEKALHAIFPQIRTMRIDADTTKHKGSHQKLLRDFGTGKADVLIGTQMIAK
GLHFPEVTLVGVLNSDAGLNIPDFRASETIFQLITQVAGRSGRGVTKGEVIIQTSMPENSTIQHAAKQDYAGFYEEEIAV
REMFHYPPFSHLAKLTFSGKQPDQTYEIAQKFRELLIFQLPQHYEFNPVIPCGYAKIKDLYRYQFLLRGPSMIPLNLVLE
NLMQKFSIPKEIKLFVDINPSSTFF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=746, Percent_Identity=34.7184986595174, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787357, Length=194, Percent_Identity=29.3814432989691, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259
- InterPro:   IPR006935 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII [H]

EC number: NA

Molecular weight: Translated: 84054; Mature: 84054

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFAAIASVILDISIDKTLDYGIVPSQLEAAQKGSRVEVPVRGHSRTGYIVEIKSDSNF
CCHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCC
KNIKPITRLLSETPLVPEELFKLALWVAKYYCAPLRDIFRIILPPSVRKGMKEKEQLFVV
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEE
RGKSKEILRQVCVDIREKKPAQASVLEVMLQVKNGILLSKLLEESKSSRSSVLSLAKQQL
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHE
LIVETIKIDRSPLVDEDYFMTKPKILNEDQAVALNKIDASLNAKIFQTHLIHGITGSGKT
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCH
EIYLQAIEKALKLEKGTIMLVPEISLTAQTIQRFRSRFAEKIAILHHRLSEGERRDEWHK
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IREGRAKIIVGARSAIFSPVVNLGLIIVDEEHEQSYKQNEMSPCYQARDVAVMRGKLAQA
HHCCCEEEEEECHHHHHHHHHHCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCHHEE
TVVLGSATPSLESYYNALNGKYALSVLHKRADVATLPDVTIVDMRKEFERAKTLTSFSEV
EEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSGIERRQKQGEQVILFLNRRGYHTTLLCQDCSHIVKCTHCEIPLTFHLGDNHLSCHLC
HHHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHHHHHEEEECCCEEEEEECCCCEEEEEE
GYQISPPPKECPSCRGTKHLKFRGAGTEHVEKALHAIFPQIRTMRIDADTTKHKGSHQKL
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
LRDFGTGKADVLIGTQMIAKGLHFPEVTLVGVLNSDAGLNIPDFRASETIFQLITQVAGR
HHHHCCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
SGRGVTKGEVIIQTSMPENSTIQHAAKQDYAGFYEEEIAVREMFHYPPFSHLAKLTFSGK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHEECCC
QPDQTYEIAQKFRELLIFQLPQHYEFNPVIPCGYAKIKDLYRYQFLLRGPSMIPLNLVLE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
NLMQKFSIPKEIKLFVDINPSSTFF
HHHHHHCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKTFAAIASVILDISIDKTLDYGIVPSQLEAAQKGSRVEVPVRGHSRTGYIVEIKSDSNF
CCHHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCCC
KNIKPITRLLSETPLVPEELFKLALWVAKYYCAPLRDIFRIILPPSVRKGMKEKEQLFVV
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEE
RGKSKEILRQVCVDIREKKPAQASVLEVMLQVKNGILLSKLLEESKSSRSSVLSLAKQQL
ECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHE
LIVETIKIDRSPLVDEDYFMTKPKILNEDQAVALNKIDASLNAKIFQTHLIHGITGSGKT
EEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCH
EIYLQAIEKALKLEKGTIMLVPEISLTAQTIQRFRSRFAEKIAILHHRLSEGERRDEWHK
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IREGRAKIIVGARSAIFSPVVNLGLIIVDEEHEQSYKQNEMSPCYQARDVAVMRGKLAQA
HHCCCEEEEEECHHHHHHHHHHCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCHHEE
TVVLGSATPSLESYYNALNGKYALSVLHKRADVATLPDVTIVDMRKEFERAKTLTSFSEV
EEEEECCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSGIERRQKQGEQVILFLNRRGYHTTLLCQDCSHIVKCTHCEIPLTFHLGDNHLSCHLC
HHHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHHHHHEEEECCCEEEEEECCCCEEEEEE
GYQISPPPKECPSCRGTKHLKFRGAGTEHVEKALHAIFPQIRTMRIDADTTKHKGSHQKL
CCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
LRDFGTGKADVLIGTQMIAKGLHFPEVTLVGVLNSDAGLNIPDFRASETIFQLITQVAGR
HHHHCCCCEEEEECHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
SGRGVTKGEVIIQTSMPENSTIQHAAKQDYAGFYEEEIAVREMFHYPPFSHLAKLTFSGK
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHEECCC
QPDQTYEIAQKFRELLIFQLPQHYEFNPVIPCGYAKIKDLYRYQFLLRGPSMIPLNLVLE
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
NLMQKFSIPKEIKLFVDINPSSTFF
HHHHHHCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]