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The map label for this gene is menD

Identifier: 46446702

GI number: 46446702

Start: 1274130

End: 1275692

Strand: Reverse

Name: menD

Synonym: pc1068

Alternate gene names: 46446702

Gene position: 1275692-1274130 (Counterclockwise)

Preceding gene: 46446703

Following gene: 46446701

Centisome position: 52.84

GC content: 37.56

Gene sequence:

>1563_bases
ATGAATCATGAGTTGGCTTTACACGTTATTGAACAAGCCCTTCAATATGGAATCAAAGAATTTTGTGTAGCGCCCGGGGC
TCGTAATGCAGCTTTCGTTTATCTTTTAGCGCAAATTCCAACTATTAAAGTTTATTATTGGAGTGAGGAAAGATCAGCGG
CATTTTTCGCTGTGGGTCGAATTAAAGCTACTCATCAACCTATTGCTGTCGTGACAACTTCAGGAACAGCTGTTGCAGAG
CTCCTTCCAGCAACCATTGAAGCGTATTATACAAGCCTACCTTTACTGTTGATCACAGCTGATCGTCCAAGGCGATTTCG
ATTGTCTGGAGCACCTCAATCCATTGAGCAAGTAGGAATTTTTAGTTGTTATACAATTTTAGAGAAAGACTTAGCAGAAA
ATGAGTTTTTTCAACTTGATCAATGGTCAGGTAGAGGCCCTTGTCATTTAAATGTCTGTTTTGAAGAACCCTCCAAAAAT
GATAACCAGGAATGTGCTCGAAGTTTTTCGATGGGAATTTTTACTCCAAAACCTATTTTGTGTGATAGTCATTTACTCGA
TGAATTTATTCAAAAGTGTCAGTTTCCATTGGTGATTGTTGGAGCTTTAAATCAAAATGAAACAACACCCATTCTCTCGT
TTTTACGCCATTTAAAAGCACCAGTGTATTTAGAGGCGCAATCAGGTTTAAGAGAAAACTCTTTGTTAGAAAATTTGAGA
ATTGAATCGGTGGAAGATGTCTGGAATTGTTCGAGTCAGCATGGTTATCCTATTGATGGAATTCTTCGGATTGGAGGAAT
TCCAACAGCGAGATTATGGAGAGATTTGGAAGAGACAAATAACGTGAAGGTTTGCTCTATTAGTGAAAATCCGTTTGCTG
GATTAACGAACGGAAATATTATTCATACCTCTTTAAGAGAATTTTTTAAAAACTATAGACTCAGTTTTAAACCTATTTAT
TCTTTTCAAGCTTGGAAAGATGCTAATCGATCGTATCAATTGGAATTATTGAAACTTTTTGAAGAAGAGCCGCTTGCTGA
GCTAAGCCTTTTTCATTTACTATCTAAAAAAATTCCGGAAGAATCGAAAATTTATGTAGGTAATAGCCTTCCGATTCGAG
AATGGGATCAAGCTGCAGACTGGACAAATAAGCACTTCCAAGTTTTTGCTAATCGAGGTGCCAATGGAATTGAGGGACAG
TTATCAACATTTCTTGGATTAGCAAGTCCTGAGCAAAACAATTGGGCAATTTTGGGAGATTTAACCACTCTTTATGACAT
GATTGCCCCTTGGGTGATCCACCAAATTCAGCCACTAAAAGTGAATGTTGTGGTTGTCAATAATAGTGGTGGACAAATTT
TTGCACCTAAATTTGCTCATCTGTCCTTTCTTAACTCCCATCAATTAAATTTTAAAGCTTTCGCAGATTTTTGGGGTTGG
GAATACGATTGTTGGCATGAAATTCCTACAACTATTCTTGGTAGTCAAACAAGTCGATTAATTGAACTTGTCCCTGATCC
CTCTGCTACAGATCGATTAAGAAAGAAAATGCAGAGGATGTAA

Upstream 100 bases:

>100_bases
AAATAGGAGCTGGTTGTGGTGTGGTATCAGAAAGTCAGTTAGATCAAGAGTGGGAGGAACTTTGTTTAAAATTACAAGCT
GTTAAAAATGGATTGGATTT

Downstream 100 bases:

>100_bases
TGGGGAATCTTCCTTATATTTTATGGGCAATTCCCGGCTTTTTAGGCCTCCCATCGGATTGGGACCTGTTTAAATTTACA
CAAATAAAAAAAATCGATGT

Product: putative menaquinone biosynthesis protein, menD

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MNHELALHVIEQALQYGIKEFCVAPGARNAAFVYLLAQIPTIKVYYWSEERSAAFFAVGRIKATHQPIAVVTTSGTAVAE
LLPATIEAYYTSLPLLLITADRPRRFRLSGAPQSIEQVGIFSCYTILEKDLAENEFFQLDQWSGRGPCHLNVCFEEPSKN
DNQECARSFSMGIFTPKPILCDSHLLDEFIQKCQFPLVIVGALNQNETTPILSFLRHLKAPVYLEAQSGLRENSLLENLR
IESVEDVWNCSSQHGYPIDGILRIGGIPTARLWRDLEETNNVKVCSISENPFAGLTNGNIIHTSLREFFKNYRLSFKPIY
SFQAWKDANRSYQLELLKLFEEEPLAELSLFHLLSKKIPEESKIYVGNSLPIREWDQAADWTNKHFQVFANRGANGIEGQ
LSTFLGLASPEQNNWAILGDLTTLYDMIAPWVIHQIQPLKVNVVVVNNSGGQIFAPKFAHLSFLNSHQLNFKAFADFWGW
EYDCWHEIPTTILGSQTSRLIELVPDPSATDRLRKKMQRM

Sequences:

>Translated_520_residues
MNHELALHVIEQALQYGIKEFCVAPGARNAAFVYLLAQIPTIKVYYWSEERSAAFFAVGRIKATHQPIAVVTTSGTAVAE
LLPATIEAYYTSLPLLLITADRPRRFRLSGAPQSIEQVGIFSCYTILEKDLAENEFFQLDQWSGRGPCHLNVCFEEPSKN
DNQECARSFSMGIFTPKPILCDSHLLDEFIQKCQFPLVIVGALNQNETTPILSFLRHLKAPVYLEAQSGLRENSLLENLR
IESVEDVWNCSSQHGYPIDGILRIGGIPTARLWRDLEETNNVKVCSISENPFAGLTNGNIIHTSLREFFKNYRLSFKPIY
SFQAWKDANRSYQLELLKLFEEEPLAELSLFHLLSKKIPEESKIYVGNSLPIREWDQAADWTNKHFQVFANRGANGIEGQ
LSTFLGLASPEQNNWAILGDLTTLYDMIAPWVIHQIQPLKVNVVVVNNSGGQIFAPKFAHLSFLNSHQLNFKAFADFWGW
EYDCWHEIPTTILGSQTSRLIELVPDPSATDRLRKKMQRM
>Mature_520_residues
MNHELALHVIEQALQYGIKEFCVAPGARNAAFVYLLAQIPTIKVYYWSEERSAAFFAVGRIKATHQPIAVVTTSGTAVAE
LLPATIEAYYTSLPLLLITADRPRRFRLSGAPQSIEQVGIFSCYTILEKDLAENEFFQLDQWSGRGPCHLNVCFEEPSKN
DNQECARSFSMGIFTPKPILCDSHLLDEFIQKCQFPLVIVGALNQNETTPILSFLRHLKAPVYLEAQSGLRENSLLENLR
IESVEDVWNCSSQHGYPIDGILRIGGIPTARLWRDLEETNNVKVCSISENPFAGLTNGNIIHTSLREFFKNYRLSFKPIY
SFQAWKDANRSYQLELLKLFEEEPLAELSLFHLLSKKIPEESKIYVGNSLPIREWDQAADWTNKHFQVFANRGANGIEGQ
LSTFLGLASPEQNNWAILGDLTTLYDMIAPWVIHQIQPLKVNVVVVNNSGGQIFAPKFAHLSFLNSHQLNFKAFADFWGW
EYDCWHEIPTTILGSQTSRLIELVPDPSATDRLRKKMQRM

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=568, Percent_Identity=26.056338028169, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PARUW (Q6MCA7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008067.1
- ProteinModelPortal:   Q6MCA7
- STRING:   Q6MCA7
- GeneID:   2780907
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1068
- NMPDR:   fig|264201.1.peg.1068
- eggNOG:   COG1165
- HOGENOM:   HBG051164
- OMA:   TISLARY
- PhylomeDB:   Q6MCA7
- ProtClustDB:   CLSK736866
- BioCyc:   CPRO264201:PC1068-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 59015; Mature: 59015

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHELALHVIEQALQYGIKEFCVAPGARNAAFVYLLAQIPTIKVYYWSEERSAAFFAVGR
CCCHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEEEEEE
IKATHQPIAVVTTSGTAVAELLPATIEAYYTSLPLLLITADRPRRFRLSGAPQSIEQVGI
EEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCHHHHHHHH
FSCYTILEKDLAENEFFQLDQWSGRGPCHLNVCFEEPSKNDNQECARSFSMGIFTPKPIL
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCC
CDSHLLDEFIQKCQFPLVIVGALNQNETTPILSFLRHLKAPVYLEAQSGLRENSLLENLR
CCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
IESVEDVWNCSSQHGYPIDGILRIGGIPTARLWRDLEETNNVKVCSISENPFAGLTNGNI
CCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
IHTSLREFFKNYRLSFKPIYSFQAWKDANRSYQLELLKLFEEEPLAELSLFHLLSKKIPE
EHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
ESKIYVGNSLPIREWDQAADWTNKHFQVFANRGANGIEGQLSTFLGLASPEQNNWAILGD
CCEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEC
LTTLYDMIAPWVIHQIQPLKVNVVVVNNSGGQIFAPKFAHLSFLNSHQLNFKAFADFWGW
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEECCHHHHHHHHCCCCCCCEEHHHHHCC
EYDCWHEIPTTILGSQTSRLIELVPDPSATDRLRKKMQRM
CCHHHHHCCHHHHCCCCCHHEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNHELALHVIEQALQYGIKEFCVAPGARNAAFVYLLAQIPTIKVYYWSEERSAAFFAVGR
CCCHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEEEEEE
IKATHQPIAVVTTSGTAVAELLPATIEAYYTSLPLLLITADRPRRFRLSGAPQSIEQVGI
EEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCHHHHHHHH
FSCYTILEKDLAENEFFQLDQWSGRGPCHLNVCFEEPSKNDNQECARSFSMGIFTPKPIL
HHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCC
CDSHLLDEFIQKCQFPLVIVGALNQNETTPILSFLRHLKAPVYLEAQSGLRENSLLENLR
CCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHCC
IESVEDVWNCSSQHGYPIDGILRIGGIPTARLWRDLEETNNVKVCSISENPFAGLTNGNI
CCCHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCE
IHTSLREFFKNYRLSFKPIYSFQAWKDANRSYQLELLKLFEEEPLAELSLFHLLSKKIPE
EHHHHHHHHHHCCCEEEEEEEEHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
ESKIYVGNSLPIREWDQAADWTNKHFQVFANRGANGIEGQLSTFLGLASPEQNNWAILGD
CCEEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCEEEEEC
LTTLYDMIAPWVIHQIQPLKVNVVVVNNSGGQIFAPKFAHLSFLNSHQLNFKAFADFWGW
HHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEECCHHHHHHHHCCCCCCCEEHHHHHCC
EYDCWHEIPTTILGSQTSRLIELVPDPSATDRLRKKMQRM
CCHHHHHCCHHHHCCCCCHHEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA