Click here to switch to the map view.

The map label for this gene is menF [H]

Identifier: 46446703

GI number: 46446703

Start: 1275689

End: 1276744

Strand: Reverse

Name: menF [H]

Synonym: pc1069

Alternate gene names: 46446703

Gene position: 1276744-1275689 (Counterclockwise)

Preceding gene: 46446704

Following gene: 46446702

Centisome position: 52.88

GC content: 35.61

Gene sequence:

>1056_bases
GTGATAATAGATCAACGGTTTTTGAAGTCAGGAGCTATCGCAAGTCTGAGTCCAGATTTGATTTTGTTGGCTTTTGGCTC
TCGGCAATCATATGCTTCTTCTAAAGATTTGGATCTGACTGTGCCGGCTTTCTATTATCCCGATTTTTTTTTGAAGACTT
CTTTTCCTTGGCTTCAATTTTCTGATTGGAAAATAATTGAAACTAAATACTTAATCAACCTTTTGAATCCACCCGCTATT
TTACGGTCGGAATGGATGATTGAAAATCAATTCTTATTTCAAGAAAACTTCTATCAATTAAAGCAATTATTTGAACAAAA
ACAATTAGAAAAAGCCGTTCCCTATATTTTTGCCCACTCTCATACAAAGATGACACTAAATCAATTAAAAACATCTCTTA
AAAAAGCATTAGAACAGCTCTCTATATCATCCGTTTATTTATATGGATGTTGGGATAGCGAAAAAGGTATCTTAGGTCTA
ACTCCGGAAATATTATTTGAATGGAATAACCAAAATACCCATTTAGTCAAAACAATGGCTTTGGCAGGGACGAAAAAATT
GGAAAATGCAAATCCTTTTTCATTAGATAGAAAAGAGTTGTTTGAACATCAGGTAGTTGTTGAAGGAATTTGTGAAAGTT
TAGCATCGCTTGGGAAGGTTTCTATAGGACAGCGACAGCTTTTACAACTTCCTATATTAACTCATTTAATGACCCCTATT
GAAATGTCTCTATATCAACCGACTTCTTTTGAAGTTCTAGTTAGAGCTTTGCATCCAACACCTGCTCTTGGAGCTTTTCC
GAGACAAAAAGGAGAGGAGTGGTTGTGCCACTATCAAAATCAATTGGATCGAAATTCGTTTGGAGCGCCAGTCGGGCTCA
GTTTTCCAGAAAAAGGGATTAAAAATTGTTATGTTGCTATACGCAATGTCCAATGGAATGATCAAGGAATGAAAATAGGA
GCTGGTTGTGGTGTGGTATCAGAAAGTCAGTTAGATCAAGAGTGGGAGGAACTTTGTTTAAAATTACAAGCTGTTAAAAA
TGGATTGGATTTATGA

Upstream 100 bases:

>100_bases
TGATATTTTGATAGAATCAACTTTTGAAAATAGTGATTGATTCATAACCTGAATAATCCTTTGGGTTATGATCTTTAAAT
TGATTCTTCAGAGGAATTTT

Downstream 100 bases:

>100_bases
ATCATGAGTTGGCTTTACACGTTATTGAACAAGCCCTTCAATATGGAATCAAAGAATTTTGTGTAGCGCCCGGGGCTCGT
AATGCAGCTTTCGTTTATCT

Product: putative menaquinone-specific isochorismate synthase, menF

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIIDQRFLKSGAIASLSPDLILLAFGSRQSYASSKDLDLTVPAFYYPDFFLKTSFPWLQFSDWKIIETKYLINLLNPPAI
LRSEWMIENQFLFQENFYQLKQLFEQKQLEKAVPYIFAHSHTKMTLNQLKTSLKKALEQLSISSVYLYGCWDSEKGILGL
TPEILFEWNNQNTHLVKTMALAGTKKLENANPFSLDRKELFEHQVVVEGICESLASLGKVSIGQRQLLQLPILTHLMTPI
EMSLYQPTSFEVLVRALHPTPALGAFPRQKGEEWLCHYQNQLDRNSFGAPVGLSFPEKGIKNCYVAIRNVQWNDQGMKIG
AGCGVVSESQLDQEWEELCLKLQAVKNGLDL

Sequences:

>Translated_351_residues
MIIDQRFLKSGAIASLSPDLILLAFGSRQSYASSKDLDLTVPAFYYPDFFLKTSFPWLQFSDWKIIETKYLINLLNPPAI
LRSEWMIENQFLFQENFYQLKQLFEQKQLEKAVPYIFAHSHTKMTLNQLKTSLKKALEQLSISSVYLYGCWDSEKGILGL
TPEILFEWNNQNTHLVKTMALAGTKKLENANPFSLDRKELFEHQVVVEGICESLASLGKVSIGQRQLLQLPILTHLMTPI
EMSLYQPTSFEVLVRALHPTPALGAFPRQKGEEWLCHYQNQLDRNSFGAPVGLSFPEKGIKNCYVAIRNVQWNDQGMKIG
AGCGVVSESQLDQEWEELCLKLQAVKNGLDL
>Mature_351_residues
MIIDQRFLKSGAIASLSPDLILLAFGSRQSYASSKDLDLTVPAFYYPDFFLKTSFPWLQFSDWKIIETKYLINLLNPPAI
LRSEWMIENQFLFQENFYQLKQLFEQKQLEKAVPYIFAHSHTKMTLNQLKTSLKKALEQLSISSVYLYGCWDSEKGILGL
TPEILFEWNNQNTHLVKTMALAGTKKLENANPFSLDRKELFEHQVVVEGICESLASLGKVSIGQRQLLQLPILTHLMTPI
EMSLYQPTSFEVLVRALHPTPALGAFPRQKGEEWLCHYQNQLDRNSFGAPVGLSFPEKGIKNCYVAIRNVQWNDQGMKIG
AGCGVVSESQLDQEWEELCLKLQAVKNGLDL

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=201, Percent_Identity=28.8557213930348, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082077, Length=224, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 40044; Mature: 40044

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIDQRFLKSGAIASLSPDLILLAFGSRQSYASSKDLDLTVPAFYYPDFFLKTSFPWLQF
CCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECHHCCCCHHEECCCCCEEE
SDWKIIETKYLINLLNPPAILRSEWMIENQFLFQENFYQLKQLFEQKQLEKAVPYIFAHS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
HTKMTLNQLKTSLKKALEQLSISSVYLYGCWDSEKGILGLTPEILFEWNNQNTHLVKTMA
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEECCHHHEEEECCCCCEEEHHHH
LAGTKKLENANPFSLDRKELFEHQVVVEGICESLASLGKVSIGQRQLLQLPILTHLMTPI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCH
EMSLYQPTSFEVLVRALHPTPALGAFPRQKGEEWLCHYQNQLDRNSFGAPVGLSFPEKGI
HHEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
KNCYVAIRNVQWNDQGMKIGAGCGVVSESQLDQEWEELCLKLQAVKNGLDL
HHHEEEEECCEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIIDQRFLKSGAIASLSPDLILLAFGSRQSYASSKDLDLTVPAFYYPDFFLKTSFPWLQF
CCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECHHCCCCHHEECCCCCEEE
SDWKIIETKYLINLLNPPAILRSEWMIENQFLFQENFYQLKQLFEQKQLEKAVPYIFAHS
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
HTKMTLNQLKTSLKKALEQLSISSVYLYGCWDSEKGILGLTPEILFEWNNQNTHLVKTMA
CHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEECCHHHEEEECCCCCEEEHHHH
LAGTKKLENANPFSLDRKELFEHQVVVEGICESLASLGKVSIGQRQLLQLPILTHLMTPI
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCH
EMSLYQPTSFEVLVRALHPTPALGAFPRQKGEEWLCHYQNQLDRNSFGAPVGLSFPEKGI
HHEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
KNCYVAIRNVQWNDQGMKIGAGCGVVSESQLDQEWEELCLKLQAVKNGLDL
HHHEEEEECCEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]