Click here to switch to the map view.

The map label for this gene is menH [H]

Identifier: 46446701

GI number: 46446701

Start: 1273420

End: 1274130

Strand: Reverse

Name: menH [H]

Synonym: pc1067

Alternate gene names: 46446701

Gene position: 1274130-1273420 (Counterclockwise)

Preceding gene: 46446702

Following gene: 46446698

Centisome position: 52.77

GC content: 36.57

Gene sequence:

>711_bases
ATGGGGAATCTTCCTTATATTTTATGGGCAATTCCCGGCTTTTTAGGCCTCCCATCGGATTGGGACCTGTTTAAATTTAC
ACAAATAAAAAAAATCGATGTGAACTCTTTTTCGTGTACAGATTTATCGAGTTGGGCAGAGCATTTTAATGCATTTGTTA
AAAAACAACCTCCTCATTTTCCTATTTTGCTCGGTTATTCGATGGGGGGAAGATTGGGATTACATGCGTTGTGTCAAGAT
CCAGATTTATGGAAAGGAGGGATTCTGATTTCTACCCATCCAGGTTTATCTCAAGAAAACGAAAAAGAAGAACGGTTGGA
TCAGGATGGAAAATGGGCCGGTCTTTTTGAAACTGAAGAATGGGAATCTTTAATAAAGCAATGGAATGCACAAGATATAT
TTGCGAAAGACCTCATTTTTTATCGTTCCCATAATGATTATCAGCGGTCGAATCTAGCTATGCACCTAAGAAATTTTTCA
TTAGGGAAACAAGAAGATTTAAAAGAAAAAATAGGCCTACTTCCTTTTCCCATTTTATGGATGACTGGTGAGAAAGATAA
AAAATTTTCTCAATTAGGCCAACAGCTTTATTTTAAACATCCGTTATCTTTCCACTTATCTGTTAAAGGAGCGGGGCATA
GAGTTCCTTGGCTCTGTCCTGATATCTTTAAATCGCAGTTATCAATTTTTATTGAAGCATTGACAATTTGA

Upstream 100 bases:

>100_bases
TTCCTACAACTATTCTTGGTAGTCAAACAAGTCGATTAATTGAACTTGTCCCTGATCCCTCTGCTACAGATCGATTAAGA
AAGAAAATGCAGAGGATGTA

Downstream 100 bases:

>100_bases
TTTTATTCATAAGTTTTAATCAATTGTTTTGTAAGTAGAAAATTTCTTTCCATTTTCCTGAGCGATCAAGTGCAGAAAAA
AATTGAAGAAGGCAAAGATG

Product: hypothetical protein

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MGNLPYILWAIPGFLGLPSDWDLFKFTQIKKIDVNSFSCTDLSSWAEHFNAFVKKQPPHFPILLGYSMGGRLGLHALCQD
PDLWKGGILISTHPGLSQENEKEERLDQDGKWAGLFETEEWESLIKQWNAQDIFAKDLIFYRSHNDYQRSNLAMHLRNFS
LGKQEDLKEKIGLLPFPILWMTGEKDKKFSQLGQQLYFKHPLSFHLSVKGAGHRVPWLCPDIFKSQLSIFIEALTI

Sequences:

>Translated_236_residues
MGNLPYILWAIPGFLGLPSDWDLFKFTQIKKIDVNSFSCTDLSSWAEHFNAFVKKQPPHFPILLGYSMGGRLGLHALCQD
PDLWKGGILISTHPGLSQENEKEERLDQDGKWAGLFETEEWESLIKQWNAQDIFAKDLIFYRSHNDYQRSNLAMHLRNFS
LGKQEDLKEKIGLLPFPILWMTGEKDKKFSQLGQQLYFKHPLSFHLSVKGAGHRVPWLCPDIFKSQLSIFIEALTI
>Mature_235_residues
GNLPYILWAIPGFLGLPSDWDLFKFTQIKKIDVNSFSCTDLSSWAEHFNAFVKKQPPHFPILLGYSMGGRLGLHALCQDP
DLWKGGILISTHPGLSQENEKEERLDQDGKWAGLFETEEWESLIKQWNAQDIFAKDLIFYRSHNDYQRSNLAMHLRNFSL
GKQEDLKEKIGLLPFPILWMTGEKDKKFSQLGQQLYFKHPLSFHLSVKGAGHRVPWLCPDIFKSQLSIFIEALTI

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=141, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 27237; Mature: 27105

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNLPYILWAIPGFLGLPSDWDLFKFTQIKKIDVNSFSCTDLSSWAEHFNAFVKKQPPHF
CCCCCEEHEECCHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC
PILLGYSMGGRLGLHALCQDPDLWKGGILISTHPGLSQENEKEERLDQDGKWAGLFETEE
EEEEEECCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCEEECCCCHH
WESLIKQWNAQDIFAKDLIFYRSHNDYQRSNLAMHLRNFSLGKQEDLKEKIGLLPFPILW
HHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEE
MTGEKDKKFSQLGQQLYFKHPLSFHLSVKGAGHRVPWLCPDIFKSQLSIFIEALTI
EECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNLPYILWAIPGFLGLPSDWDLFKFTQIKKIDVNSFSCTDLSSWAEHFNAFVKKQPPHF
CCCCEEHEECCHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCC
PILLGYSMGGRLGLHALCQDPDLWKGGILISTHPGLSQENEKEERLDQDGKWAGLFETEE
EEEEEECCCCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCEEECCCCHH
WESLIKQWNAQDIFAKDLIFYRSHNDYQRSNLAMHLRNFSLGKQEDLKEKIGLLPFPILW
HHHHHHHCCCCHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEE
MTGEKDKKFSQLGQQLYFKHPLSFHLSVKGAGHRVPWLCPDIFKSQLSIFIEALTI
EECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA