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The map label for this gene is rlmI [H]

Identifier: 46446677

GI number: 46446677

Start: 1241557

End: 1242729

Strand: Reverse

Name: rlmI [H]

Synonym: pc1043

Alternate gene names: 46446677

Gene position: 1242729-1241557 (Counterclockwise)

Preceding gene: 46446678

Following gene: 46446675

Centisome position: 51.47

GC content: 35.89

Gene sequence:

>1173_bases
ATGCAACGGCATAGTGTTATTTTAAAGCCTGGTAAAGATAAAGCAATTAGGAATTATCATCATTGGATTTTTTCTGGAGC
TGTGCAATCCTATCCTTCATTTAAAGACGGCCAGATTCTTCCTGTTTGTGCTTCCTCTCACCAATTGCTAGGTTATGGAT
ATTTTAATCGTCGTTCGGGAATTGTTGGTAGAATGGTTTCGTTCAATAACGTTCCGCCATTAGAAACTCTTAAAAAACGG
CTCATTTCAGCATGGAAATTTAGACAGCAGTTATTTGATTTCAGACAAACTAATGCTTATCGTTTTGTGCATGGCGAAGG
AGATGCGATTCCTGGGTTGACGATAGACGTTTATCAAGATGTCTTTGTCTTACAAAGCTCAACTAAAGGAATTGACGAAT
TGAAGCCGTGGATTATTGAGACTCTCAACGAACTCTTTCAACCAAGATCGATTTTTGAAAAATCGATATTACCTTCTCGT
AAAGAAGAAGGATTAGCAGATGTACAAAAGCATTTATCAGGAGAAGAAGTTGGCGACATTTCATTTTTTGAAAATGGTTT
AAAATTTACAACGAATTTACATAAATCTCAAAAAACAGGCTTTTTTCTTGATCATCGCGAAATGCGCCAATGGATAAAAA
CTTTATCTTTCAACAAAAAAGTATTAAATGCTTTTTCTTACACTGGTGGTTTTAGCGTATATGCTATGGCTGGAGGAGCT
AAACGAGTAGATTCAGTAGATATTTCTCAAGAAGCTGTAAATGCTTGTCAAAAACACTTTGTTTTAAATGAACTTTCCGA
ATTTGGCAGCCGATTCATTTGTGCTGACGTCTTCAATTTTTTACGTGAAGATGTTCTTGATTATGATCTTGTTATTTTAG
ATCCTCCTGCTTTTGCAAAAAAGCAAAAAGATGTCATAGCTGCTTGCCGTGGTTATAAAGACATTAATCGATTAGCAATG
CAAAAAATGCCTAGAAGATCTCTTCTTTTGACCTGTTCTTGTTCACATCATGTTAATGAGCTTCTATTTCAAAAAGTTGT
TTTTCAGGCGGCAGCAGAAGCAAATAGAAATGTACGCCTCATCGGTAGACATCAATTAGCCTGTGACCACCCCATTAATC
TGTTTCATCCAGAAAGCGATTATTTAAAAAGCTTTCTCCTTTACCTAGATTAA

Upstream 100 bases:

>100_bases
AATAATTACACCTATTGGTGGGGCAGCTTTGCTCATAGGTTGGTTTTTTTTAATGTTATTTGGTTTTTTTGACAAATAAT
TGATTAATATAGATTTATAT

Downstream 100 bases:

>100_bases
ACTTTTAGTAAATAATCTACTCGAAAGGAATTGTCGAACTGTTTGATATTTTGAATTTTTAACAACTTGGCTTTTTTTAG
AGTCGGAATTTCCTTAGAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MQRHSVILKPGKDKAIRNYHHWIFSGAVQSYPSFKDGQILPVCASSHQLLGYGYFNRRSGIVGRMVSFNNVPPLETLKKR
LISAWKFRQQLFDFRQTNAYRFVHGEGDAIPGLTIDVYQDVFVLQSSTKGIDELKPWIIETLNELFQPRSIFEKSILPSR
KEEGLADVQKHLSGEEVGDISFFENGLKFTTNLHKSQKTGFFLDHREMRQWIKTLSFNKKVLNAFSYTGGFSVYAMAGGA
KRVDSVDISQEAVNACQKHFVLNELSEFGSRFICADVFNFLREDVLDYDLVILDPPAFAKKQKDVIAACRGYKDINRLAM
QKMPRRSLLLTCSCSHHVNELLFQKVVFQAAAEANRNVRLIGRHQLACDHPINLFHPESDYLKSFLLYLD

Sequences:

>Translated_390_residues
MQRHSVILKPGKDKAIRNYHHWIFSGAVQSYPSFKDGQILPVCASSHQLLGYGYFNRRSGIVGRMVSFNNVPPLETLKKR
LISAWKFRQQLFDFRQTNAYRFVHGEGDAIPGLTIDVYQDVFVLQSSTKGIDELKPWIIETLNELFQPRSIFEKSILPSR
KEEGLADVQKHLSGEEVGDISFFENGLKFTTNLHKSQKTGFFLDHREMRQWIKTLSFNKKVLNAFSYTGGFSVYAMAGGA
KRVDSVDISQEAVNACQKHFVLNELSEFGSRFICADVFNFLREDVLDYDLVILDPPAFAKKQKDVIAACRGYKDINRLAM
QKMPRRSLLLTCSCSHHVNELLFQKVVFQAAAEANRNVRLIGRHQLACDHPINLFHPESDYLKSFLLYLD
>Mature_390_residues
MQRHSVILKPGKDKAIRNYHHWIFSGAVQSYPSFKDGQILPVCASSHQLLGYGYFNRRSGIVGRMVSFNNVPPLETLKKR
LISAWKFRQQLFDFRQTNAYRFVHGEGDAIPGLTIDVYQDVFVLQSSTKGIDELKPWIIETLNELFQPRSIFEKSILPSR
KEEGLADVQKHLSGEEVGDISFFENGLKFTTNLHKSQKTGFFLDHREMRQWIKTLSFNKKVLNAFSYTGGFSVYAMAGGA
KRVDSVDISQEAVNACQKHFVLNELSEFGSRFICADVFNFLREDVLDYDLVILDPPAFAKKQKDVIAACRGYKDINRLAM
QKMPRRSLLLTCSCSHHVNELLFQKVVFQAAAEANRNVRLIGRHQLACDHPINLFHPESDYLKSFLLYLD

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=387, Percent_Identity=35.1421188630491, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1787181, Length=216, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44682; Mature: 44682

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRHSVILKPGKDKAIRNYHHWIFSGAVQSYPSFKDGQILPVCASSHQLLGYGYFNRRSG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
IVGRMVSFNNVPPLETLKKRLISAWKFRQQLFDFRQTNAYRFVHGEGDAIPGLTIDVYQD
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHH
VFVLQSSTKGIDELKPWIIETLNELFQPRSIFEKSILPSRKEEGLADVQKHLSGEEVGDI
HHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCH
SFFENGLKFTTNLHKSQKTGFFLDHREMRQWIKTLSFNKKVLNAFSYTGGFSVYAMAGGA
HHHHCCCCEEHHHHHCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCC
KRVDSVDISQEAVNACQKHFVLNELSEFGSRFICADVFNFLREDVLDYDLVILDPPAFAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHH
KQKDVIAACRGYKDINRLAMQKMPRRSLLLTCSCSHHVNELLFQKVVFQAAAEANRNVRL
HHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEE
IGRHQLACDHPINLFHPESDYLKSFLLYLD
EECCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQRHSVILKPGKDKAIRNYHHWIFSGAVQSYPSFKDGQILPVCASSHQLLGYGYFNRRSG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
IVGRMVSFNNVPPLETLKKRLISAWKFRQQLFDFRQTNAYRFVHGEGDAIPGLTIDVYQD
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHH
VFVLQSSTKGIDELKPWIIETLNELFQPRSIFEKSILPSRKEEGLADVQKHLSGEEVGDI
HHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHCCHHHHHHHCCCCCCCCH
SFFENGLKFTTNLHKSQKTGFFLDHREMRQWIKTLSFNKKVLNAFSYTGGFSVYAMAGGA
HHHHCCCCEEHHHHHCCCCCEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCC
KRVDSVDISQEAVNACQKHFVLNELSEFGSRFICADVFNFLREDVLDYDLVILDPPAFAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHH
KQKDVIAACRGYKDINRLAMQKMPRRSLLLTCSCSHHVNELLFQKVVFQAAAEANRNVRL
HHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEE
IGRHQLACDHPINLFHPESDYLKSFLLYLD
EECCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA