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The map label for this gene is lig [H]

Identifier: 46446584

GI number: 46446584

Start: 1147931

End: 1149523

Strand: Reverse

Name: lig [H]

Synonym: pc0950

Alternate gene names: 46446584

Gene position: 1149523-1147931 (Counterclockwise)

Preceding gene: 46446585

Following gene: 46446583

Centisome position: 47.61

GC content: 35.4

Gene sequence:

>1593_bases
ATGAAAAATTTTTCACTTCTTTTTGAACAGATTGATCAAACACAATCAACGAATGAAAAAGTCTCTTATATTCAAAAATA
TTTTGCTGATTCTTCCCCAGCCGATGGAGCATGGGCTCTTTTTTTTTTAAGCGGGCATCGTTTAAAAAGATTAATTAGTG
GAAAGAAATTATTAAAGTGGTGTCAGGAACTTTTGCAACTTCCTGATTGGTTGGTAGAAGAATCATATGCAGCCGTAGGA
GATACAGCAGAAACAATTTCTTTATTATTACCTTTTAAAGAAAAAGAAATTAAAAATAAACTGACCCTTTCCGAATGGAT
GGAAATGCGAATTAAACCACTTCAAGGTCTTCTAGAAGAAAAACAAAAAGAGCAGATTTTTCTTTACTGGAATGAACTGA
ACACTAAAGAGACTTTTATTTTTAACAAAATACTGACGGGTTCTTTTCGCGTTGGTGTTTCTTCTTTGTTAACATTAAAA
GGATTAAGCCAAGCATTAGAAATTCCTCGTGAAATTTTAAGTCAGCATGTAATGGGAACCTGGGAACCCACAGCAGAATT
TTTTGCTAGTTTGAGTATCACAGATGATCGTAAACAATATTTAAATCCTTATCCCTTTTATTTGGCTTATCCTTTTGAAG
GCGATTTAAAGACATTAGAAAATCCGTTTGATTGGTTAGCAGAATGGAAATGGGATGGGATCCGAGCACAAGTTGTCAAA
AGGGGAGGGGAATGTGCCATTTGGTCAAGAGGAAATGAATTAGTTTCTTCGCAATTTCCCGAAATTATGGAAGTTTGCAA
ACAACTTCCAGACGGGACGGTATTAGATGGAGAATTGATTGCTTATCAATCGAATCGCCCCTTAGCATTTGGAGAATTAC
AAAAACGCCTTGGAAGAAAAAATGTGTCAAAAAGTATGCAAAAAAATATTCCAGTAATTTTAATGATTTATGACGTTTTA
GAATATCGGGGGGAAGATATTCGTAAATTACCTTTGGGCGATCGAAGAGACGTTTTGAAATGTTTAAACCTAGAATCCCC
ACAGCTTCTCATTTCGGATGAAATCTCTTTTTCAAACTGGGAAGAGTTAAGCGAAAAACGAGCTTTAGCTAGACAACAAG
GGACAGAAGGAGTGATGTTAAAGAAGCGAAACTCTTTTTATGGTGTAGGACGTCAAAAAGGAAATTGGTGGAAGTATAAA
ATTGATCCGATGGTCATTGATGCTGTTTTAATGTATGCTCAAGCTGGTTCAGGTAGAAGGGCTAATTTATTTACAGATTA
TACTTTTGGTATTTGGAATCAAAATGAATTAATTCCCATTGCTAAAGCTTATTCTGGTTTAGATCAAGGGGAAATCAACA
AGTTAGATCGTTGGATCCGAATGCATACCGAAGAAAAATTTGGCCCGGTTAGAAAAGTAAAGACAGAACAGGTTTTTGAG
ATCGCTTTTGAAGGGATACAGCGTTCTAATCGCCATAAATCAGGAATAGCTCTACGATTTCCTCGCATCAAAAGATGGAG
AACTGATAAACCTTATCAAGAATGCGATGATTTAGAAACAATTATAAAAACGTTTTTACATGACTCTTCCTAA

Upstream 100 bases:

>100_bases
ACGGGAATGCTTCTACGTTGGCTCAATATTTACGCGAAACGCGAAACCTCGATGCCAGAGAGTTAAAGGGGCTAGATGTT
ACTTTAGTGAGTGAGGATCA

Downstream 100 bases:

>100_bases
GCATTCACTCATTGAACAATGGTTTAATTTTAAAAAATGGGAACCTCTTCCCTTTCAAAAAGAAGCCTGGTTAAAATTTT
TAGAAGGAAAAAGTGGGTTA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKNFSLLFEQIDQTQSTNEKVSYIQKYFADSSPADGAWALFFLSGHRLKRLISGKKLLKWCQELLQLPDWLVEESYAAVG
DTAETISLLLPFKEKEIKNKLTLSEWMEMRIKPLQGLLEEKQKEQIFLYWNELNTKETFIFNKILTGSFRVGVSSLLTLK
GLSQALEIPREILSQHVMGTWEPTAEFFASLSITDDRKQYLNPYPFYLAYPFEGDLKTLENPFDWLAEWKWDGIRAQVVK
RGGECAIWSRGNELVSSQFPEIMEVCKQLPDGTVLDGELIAYQSNRPLAFGELQKRLGRKNVSKSMQKNIPVILMIYDVL
EYRGEDIRKLPLGDRRDVLKCLNLESPQLLISDEISFSNWEELSEKRALARQQGTEGVMLKKRNSFYGVGRQKGNWWKYK
IDPMVIDAVLMYAQAGSGRRANLFTDYTFGIWNQNELIPIAKAYSGLDQGEINKLDRWIRMHTEEKFGPVRKVKTEQVFE
IAFEGIQRSNRHKSGIALRFPRIKRWRTDKPYQECDDLETIIKTFLHDSS

Sequences:

>Translated_530_residues
MKNFSLLFEQIDQTQSTNEKVSYIQKYFADSSPADGAWALFFLSGHRLKRLISGKKLLKWCQELLQLPDWLVEESYAAVG
DTAETISLLLPFKEKEIKNKLTLSEWMEMRIKPLQGLLEEKQKEQIFLYWNELNTKETFIFNKILTGSFRVGVSSLLTLK
GLSQALEIPREILSQHVMGTWEPTAEFFASLSITDDRKQYLNPYPFYLAYPFEGDLKTLENPFDWLAEWKWDGIRAQVVK
RGGECAIWSRGNELVSSQFPEIMEVCKQLPDGTVLDGELIAYQSNRPLAFGELQKRLGRKNVSKSMQKNIPVILMIYDVL
EYRGEDIRKLPLGDRRDVLKCLNLESPQLLISDEISFSNWEELSEKRALARQQGTEGVMLKKRNSFYGVGRQKGNWWKYK
IDPMVIDAVLMYAQAGSGRRANLFTDYTFGIWNQNELIPIAKAYSGLDQGEINKLDRWIRMHTEEKFGPVRKVKTEQVFE
IAFEGIQRSNRHKSGIALRFPRIKRWRTDKPYQECDDLETIIKTFLHDSS
>Mature_530_residues
MKNFSLLFEQIDQTQSTNEKVSYIQKYFADSSPADGAWALFFLSGHRLKRLISGKKLLKWCQELLQLPDWLVEESYAAVG
DTAETISLLLPFKEKEIKNKLTLSEWMEMRIKPLQGLLEEKQKEQIFLYWNELNTKETFIFNKILTGSFRVGVSSLLTLK
GLSQALEIPREILSQHVMGTWEPTAEFFASLSITDDRKQYLNPYPFYLAYPFEGDLKTLENPFDWLAEWKWDGIRAQVVK
RGGECAIWSRGNELVSSQFPEIMEVCKQLPDGTVLDGELIAYQSNRPLAFGELQKRLGRKNVSKSMQKNIPVILMIYDVL
EYRGEDIRKLPLGDRRDVLKCLNLESPQLLISDEISFSNWEELSEKRALARQQGTEGVMLKKRNSFYGVGRQKGNWWKYK
IDPMVIDAVLMYAQAGSGRRANLFTDYTFGIWNQNELIPIAKAYSGLDQGEINKLDRWIRMHTEEKFGPVRKVKTEQVFE
IAFEGIQRSNRHKSGIALRFPRIKRWRTDKPYQECDDLETIIKTFLHDSS

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=350, Percent_Identity=26, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI73747829, Length=333, Percent_Identity=24.6246246246246, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI73747844, Length=333, Percent_Identity=24.6246246246246, Blast_Score=73, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71985269, Length=341, Percent_Identity=27.2727272727273, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320038, Length=347, Percent_Identity=25.3602305475504, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85815841, Length=592, Percent_Identity=23.4797297297297, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24762424, Length=360, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 61583; Mature: 61583

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNFSLLFEQIDQTQSTNEKVSYIQKYFADSSPADGAWALFFLSGHRLKRLISGKKLLKW
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
CQELLQLPDWLVEESYAAVGDTAETISLLLPFKEKEIKNKLTLSEWMEMRIKPLQGLLEE
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KQKEQIFLYWNELNTKETFIFNKILTGSFRVGVSSLLTLKGLSQALEIPREILSQHVMGT
HCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WEPTAEFFASLSITDDRKQYLNPYPFYLAYPFEGDLKTLENPFDWLAEWKWDGIRAQVVK
CCHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCHHHHCCHHHHHHHCCCCCHHHHHHH
RGGECAIWSRGNELVSSQFPEIMEVCKQLPDGTVLDGELIAYQSNRPLAFGELQKRLGRK
CCCCEEEECCCCHHHHHCCHHHHHHHHHCCCCCEECCEEEEEECCCCCCHHHHHHHHHHH
NVSKSMQKNIPVILMIYDVLEYRGEDIRKLPLGDRRDVLKCLNLESPQLLISDEISFSNW
HHHHHHHCCCCEEEHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCEEEECCCCCCCH
EELSEKRALARQQGTEGVMLKKRNSFYGVGRQKGNWWKYKIDPMVIDAVLMYAQAGSGRR
HHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCC
ANLFTDYTFGIWNQNELIPIAKAYSGLDQGEINKLDRWIRMHTEEKFGPVRKVKTEQVFE
CCEEEEEEEEECCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IAFEGIQRSNRHKSGIALRFPRIKRWRTDKPYQECDDLETIIKTFLHDSS
HHHHHHHHCCCCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNFSLLFEQIDQTQSTNEKVSYIQKYFADSSPADGAWALFFLSGHRLKRLISGKKLLKW
CCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHH
CQELLQLPDWLVEESYAAVGDTAETISLLLPFKEKEIKNKLTLSEWMEMRIKPLQGLLEE
HHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KQKEQIFLYWNELNTKETFIFNKILTGSFRVGVSSLLTLKGLSQALEIPREILSQHVMGT
HCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WEPTAEFFASLSITDDRKQYLNPYPFYLAYPFEGDLKTLENPFDWLAEWKWDGIRAQVVK
CCHHHHHHHHCCCCCCHHHHCCCCCEEEEECCCCCHHHHCCHHHHHHHCCCCCHHHHHHH
RGGECAIWSRGNELVSSQFPEIMEVCKQLPDGTVLDGELIAYQSNRPLAFGELQKRLGRK
CCCCEEEECCCCHHHHHCCHHHHHHHHHCCCCCEECCEEEEEECCCCCCHHHHHHHHHHH
NVSKSMQKNIPVILMIYDVLEYRGEDIRKLPLGDRRDVLKCLNLESPQLLISDEISFSNW
HHHHHHHCCCCEEEHHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCEEEECCCCCCCH
EELSEKRALARQQGTEGVMLKKRNSFYGVGRQKGNWWKYKIDPMVIDAVLMYAQAGSGRR
HHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCCEEEEEECHHHHHHHHHHHHCCCCCC
ANLFTDYTFGIWNQNELIPIAKAYSGLDQGEINKLDRWIRMHTEEKFGPVRKVKTEQVFE
CCEEEEEEEEECCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
IAFEGIQRSNRHKSGIALRFPRIKRWRTDKPYQECDDLETIIKTFLHDSS
HHHHHHHHCCCCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA