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The map label for this gene is mscL [H]

Identifier: 46446427

GI number: 46446427

Start: 973697

End: 974098

Strand: Reverse

Name: mscL [H]

Synonym: pc0793

Alternate gene names: 46446427

Gene position: 974098-973697 (Counterclockwise)

Preceding gene: 46446428

Following gene: 46446426

Centisome position: 40.34

GC content: 36.57

Gene sequence:

>402_bases
ATGACTTGGTTAAAAGAGTTTAAAGAATTCGCCCTTCGTGGCAATAGGGTGGATTTAGCTATTGGAATCATCATCGGTGG
GGTATTTAGCAAAGCCGTTAATTCTTTAGTCACCGATGTCATTATGCCTTTAATTGGTCTTCTATTAGGAGGCGCTGATT
TTAGTGCTCTATCCTTAAAATTACATTTACCAGAAAGTGCTGCAGCTCCAGTTGCAGTAAAATATGGAATTTTCGTCAAC
ACTTTGGTTGACCTACTGATTCTTTCTTCTGTAATCTTTATTGTGATCAAAGGAATGAACCGATTATATGAGAAAAGAAG
TAAAGCTTCTTGTACAAGAGAATGTCCAGAATGTTTAATGACTATACCAATTCAAGCTAAAAATGTGGACATTGCACTCT
AG

Upstream 100 bases:

>100_bases
TTTATTTTTTTCTCCTTTTATTAATACATCAATCCAACTTCATTGACATAATTCCCCTTTTTTAAGCATATAAAGTTAAT
TGCTTAATTAGGAGATTATG

Downstream 100 bases:

>100_bases
ACCTCGCTCAAAAAAAAGATTATTTAACTCATGTATTCAACTGAATTAGCTATTTTGGGTGGGCCAAAAGCTTTTAAAAA
AGCTTTTGAAGAAAAATGGC

Product: putative mechanosensitive channel of large conductance

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MTWLKEFKEFALRGNRVDLAIGIIIGGVFSKAVNSLVTDVIMPLIGLLLGGADFSALSLKLHLPESAAAPVAVKYGIFVN
TLVDLLILSSVIFIVIKGMNRLYEKRSKASCTRECPECLMTIPIQAKNVDIAL

Sequences:

>Translated_133_residues
MTWLKEFKEFALRGNRVDLAIGIIIGGVFSKAVNSLVTDVIMPLIGLLLGGADFSALSLKLHLPESAAAPVAVKYGIFVN
TLVDLLILSSVIFIVIKGMNRLYEKRSKASCTRECPECLMTIPIQAKNVDIAL
>Mature_132_residues
TWLKEFKEFALRGNRVDLAIGIIIGGVFSKAVNSLVTDVIMPLIGLLLGGADFSALSLKLHLPESAAAPVAVKYGIFVNT
LVDLLILSSVIFIVIKGMNRLYEKRSKASCTRECPECLMTIPIQAKNVDIAL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=125, Percent_Identity=44, Blast_Score=107, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14434; Mature: 14302

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLKEFKEFALRGNRVDLAIGIIIGGVFSKAVNSLVTDVIMPLIGLLLGGADFSALSLK
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LHLPESAAAPVAVKYGIFVNTLVDLLILSSVIFIVIKGMNRLYEKRSKASCTRECPECLM
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIPIQAKNVDIAL
HCCCCCCCCCEEC
>Mature Secondary Structure 
TWLKEFKEFALRGNRVDLAIGIIIGGVFSKAVNSLVTDVIMPLIGLLLGGADFSALSLK
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LHLPESAAAPVAVKYGIFVNTLVDLLILSSVIFIVIKGMNRLYEKRSKASCTRECPECLM
EECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIPIQAKNVDIAL
HCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA