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The map label for this gene is gatA

Identifier: 46446304

GI number: 46446304

Start: 813421

End: 814890

Strand: Reverse

Name: gatA

Synonym: pc0670

Alternate gene names: 46446304

Gene position: 814890-813421 (Counterclockwise)

Preceding gene: 46446305

Following gene: 46446303

Centisome position: 33.75

GC content: 38.1

Gene sequence:

>1470_bases
ATGTATAAATTTTCTGCCATTGAAATTCGAGACCAATTCATCCGAGGAGATGTCTCTGCTAGTGCTATTACTCAGCATTA
TTTAAATCGCATTGCTAAATATGATCAAAAAATTGGAGCTTTTCTGTCAGTTTATGAAGAACAAGCCATGATGCAAGCTA
AACAGTTAGATCAAAAAAGGCTAGCTAATCAACCCCTAGGTAAATTAGCTGGAATTCCCATCGCAATAAAAGACAATATT
CTTGTGAAAGGCGAAATATCCACTTGTGGTTCTAAATTTTTGACAAATTTCAAATCTCCTTATCAAGCCAGCGTGATTGA
GTATTTATTAGCAGAAGGGGCTGTGATTATAGGAAAAACAAATATGGATGAATTTGCTATGGGTTCTTCCACTGAGAATT
CTGCTTTGCAAAAAACTAGTAATCCTTGGAATTTAAAATGTACGCCTGGTGGATCATCTGGAGGATCTGCTGCTGCTGTT
GCTGGACGTTTATCCCCCCTTTCGTTAGGGAGTGATACAGGAGGTTCTGTCCGTCTTCCTGCATCTTTCTGTGGAGTTGT
TGGCTTTAAACCAACCTACGGAAGAGTGTCCCGCTTTGGGTTAGTTGCCTATGGCTCCTCTCTCGATCAAATTGGTCCTT
TAGCTACAAATACAGCAGATACCGCTTTATTAATGGAAGTCATCGGACGACATTGCCCGAAAGATTCAACAAGTATTGCC
TTAGGACCCGAAGATTACTTGAGTCAGTTTAAAGATTCCATCGCTGGTATGAAAATCGGAGTTCCCTGGGAGTTTTTAGA
GAATTTAGCTGATGAACCAAAAAAGATTTTTCAACAATCTGTCAATTTAATGAAACAACTAGGTGCTGAAATCGTTGATA
TTGATTTGAGCATCTTAAAATATTCTTTAGCTGTATATTATATTTTGGCCACAGCTGAAGCTTCTACGAATTTAGCAAGA
TTTGATGGAATTCGTTATGGACAACGGTCTCCAAAAGCCAAAACATTAGAAGAAGTTTATGATTTTTCTAAAGAAGAAGG
TTTTGGCGCAGAAGTAAAAAGAAGAATTTTACTTGGAACGTATGTTCTTTCGGCTGGCTATCAAGATGCTTATTACAAAA
AAGCGCAAAAAGTCCGGACGTTGATTTTAAGAAGTTACAAAGAAGCTTTTGCTAAATGCGATTTAATCGCCTCTCCTGTT
TCTCCATTTGCCGCATTTGAAATAGGAGCGATTAAAGATCCATTACAAATGTACTTAGAAGATATTTATACAATTGGTAT
CAATCTCGCAGGCCTACCAGCTGTGAGCATTCCCAATGGTTTTAGCAAAGATGGAAAACCGATGGGACTCCAATTGATTG
GGGCTCAAAAACATGATAGAGAAGTTTTAAATGCTGCCCATATCTTAGAAAAATCGCTAGCTTTTCATCCTGCTATTCCA
GAATTAGTTAAAGAGGAGACATCTGCATGA

Upstream 100 bases:

>100_bases
TTAGCAAGAGATGTCTTCTTAGCAAACTCACCATCGCATATCGGAGGAATGATTCGCGTTCCACCTGTGATTAAACAAGC
ATAACGTTCTACGAGGATTC

Downstream 100 bases:

>100_bases
GCCATAAATATTCACATCCCTCTCATTCTGACTGGGAAGCAGTGATTGGACTAGAGATCCACGTTGAATTAAATACGAAA
TCTAAATTATTTAGTGTAGC

Product: glutamyl-tRNA(Gln) amidotransferase chain A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MYKFSAIEIRDQFIRGDVSASAITQHYLNRIAKYDQKIGAFLSVYEEQAMMQAKQLDQKRLANQPLGKLAGIPIAIKDNI
LVKGEISTCGSKFLTNFKSPYQASVIEYLLAEGAVIIGKTNMDEFAMGSSTENSALQKTSNPWNLKCTPGGSSGGSAAAV
AGRLSPLSLGSDTGGSVRLPASFCGVVGFKPTYGRVSRFGLVAYGSSLDQIGPLATNTADTALLMEVIGRHCPKDSTSIA
LGPEDYLSQFKDSIAGMKIGVPWEFLENLADEPKKIFQQSVNLMKQLGAEIVDIDLSILKYSLAVYYILATAEASTNLAR
FDGIRYGQRSPKAKTLEEVYDFSKEEGFGAEVKRRILLGTYVLSAGYQDAYYKKAQKVRTLILRSYKEAFAKCDLIASPV
SPFAAFEIGAIKDPLQMYLEDIYTIGINLAGLPAVSIPNGFSKDGKPMGLQLIGAQKHDREVLNAAHILEKSLAFHPAIP
ELVKEETSA

Sequences:

>Translated_489_residues
MYKFSAIEIRDQFIRGDVSASAITQHYLNRIAKYDQKIGAFLSVYEEQAMMQAKQLDQKRLANQPLGKLAGIPIAIKDNI
LVKGEISTCGSKFLTNFKSPYQASVIEYLLAEGAVIIGKTNMDEFAMGSSTENSALQKTSNPWNLKCTPGGSSGGSAAAV
AGRLSPLSLGSDTGGSVRLPASFCGVVGFKPTYGRVSRFGLVAYGSSLDQIGPLATNTADTALLMEVIGRHCPKDSTSIA
LGPEDYLSQFKDSIAGMKIGVPWEFLENLADEPKKIFQQSVNLMKQLGAEIVDIDLSILKYSLAVYYILATAEASTNLAR
FDGIRYGQRSPKAKTLEEVYDFSKEEGFGAEVKRRILLGTYVLSAGYQDAYYKKAQKVRTLILRSYKEAFAKCDLIASPV
SPFAAFEIGAIKDPLQMYLEDIYTIGINLAGLPAVSIPNGFSKDGKPMGLQLIGAQKHDREVLNAAHILEKSLAFHPAIP
ELVKEETSA
>Mature_489_residues
MYKFSAIEIRDQFIRGDVSASAITQHYLNRIAKYDQKIGAFLSVYEEQAMMQAKQLDQKRLANQPLGKLAGIPIAIKDNI
LVKGEISTCGSKFLTNFKSPYQASVIEYLLAEGAVIIGKTNMDEFAMGSSTENSALQKTSNPWNLKCTPGGSSGGSAAAV
AGRLSPLSLGSDTGGSVRLPASFCGVVGFKPTYGRVSRFGLVAYGSSLDQIGPLATNTADTALLMEVIGRHCPKDSTSIA
LGPEDYLSQFKDSIAGMKIGVPWEFLENLADEPKKIFQQSVNLMKQLGAEIVDIDLSILKYSLAVYYILATAEASTNLAR
FDGIRYGQRSPKAKTLEEVYDFSKEEGFGAEVKRRILLGTYVLSAGYQDAYYKKAQKVRTLILRSYKEAFAKCDLIASPV
SPFAAFEIGAIKDPLQMYLEDIYTIGINLAGLPAVSIPNGFSKDGKPMGLQLIGAQKHDREVLNAAHILEKSLAFHPAIP
ELVKEETSA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=35.1888667992048, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=24.0816326530612, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI166795287, Length=155, Percent_Identity=37.4193548387097, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=445, Percent_Identity=37.5280898876404, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI17537465, Length=509, Percent_Identity=26.3261296660118, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=28.525641025641, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=26.1146496815287, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=30.1020408163265, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=196, Percent_Identity=29.5918367346939, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=196, Percent_Identity=29.5918367346939, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=459, Percent_Identity=34.2047930283224, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=27.9545454545455, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=515, Percent_Identity=24.0776699029126, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=34.9494949494949, Blast_Score=261, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24644968, Length=517, Percent_Identity=26.8858800773694, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21356731, Length=510, Percent_Identity=26.078431372549, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648435, Length=477, Percent_Identity=25.9958071278826, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648437, Length=477, Percent_Identity=25.9958071278826, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550774, Length=477, Percent_Identity=25.9958071278826, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648441, Length=422, Percent_Identity=27.4881516587678, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648439, Length=422, Percent_Identity=27.4881516587678, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24652985, Length=485, Percent_Identity=23.5051546391753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922090, Length=485, Percent_Identity=23.5051546391753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652981, Length=485, Percent_Identity=23.5051546391753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652983, Length=485, Percent_Identity=23.5051546391753, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=23.6994219653179, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PARUW (Q6MDF5)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007669.1
- ProteinModelPortal:   Q6MDF5
- SMR:   Q6MDF5
- STRING:   Q6MDF5
- GeneID:   2780575
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0670
- NMPDR:   fig|264201.1.peg.670
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   ICTVIAN
- PhylomeDB:   Q6MDF5
- ProtClustDB:   CLSK2762218
- BioCyc:   CPRO264201:PC0670-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53084; Mature: 53084

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 77-77 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKFSAIEIRDQFIRGDVSASAITQHYLNRIAKYDQKIGAFLSVYEEQAMMQAKQLDQKR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANQPLGKLAGIPIAIKDNILVKGEISTCGSKFLTNFKSPYQASVIEYLLAEGAVIIGKT
HHCCCHHHHCCCCEEEECCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECC
NMDEFAMGSSTENSALQKTSNPWNLKCTPGGSSGGSAAAVAGRLSPLSLGSDTGGSVRLP
CCHHHHCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCEECC
ASFCGVVGFKPTYGRVSRFGLVAYGSSLDQIGPLATNTADTALLMEVIGRHCPKDSTSIA
HHHHHHCCCCCCHHHHHHHHHEEECCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LGPEDYLSQFKDSIAGMKIGVPWEFLENLADEPKKIFQQSVNLMKQLGAEIVDIDLSILK
ECHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHHH
YSLAVYYILATAEASTNLARFDGIRYGQRSPKAKTLEEVYDFSKEEGFGAEVKRRILLGT
HHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
YVLSAGYQDAYYKKAQKVRTLILRSYKEAFAKCDLIASPVSPFAAFEIGAIKDPLQMYLE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCHHHHHHH
DIYTIGINLAGLPAVSIPNGFSKDGKPMGLQLIGAQKHDREVLNAAHILEKSLAFHPAIP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHH
ELVKEETSA
HHHHHHCCC
>Mature Secondary Structure
MYKFSAIEIRDQFIRGDVSASAITQHYLNRIAKYDQKIGAFLSVYEEQAMMQAKQLDQKR
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LANQPLGKLAGIPIAIKDNILVKGEISTCGSKFLTNFKSPYQASVIEYLLAEGAVIIGKT
HHCCCHHHHCCCCEEEECCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECC
NMDEFAMGSSTENSALQKTSNPWNLKCTPGGSSGGSAAAVAGRLSPLSLGSDTGGSVRLP
CCHHHHCCCCCCCHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCCCEECC
ASFCGVVGFKPTYGRVSRFGLVAYGSSLDQIGPLATNTADTALLMEVIGRHCPKDSTSIA
HHHHHHCCCCCCHHHHHHHHHEEECCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LGPEDYLSQFKDSIAGMKIGVPWEFLENLADEPKKIFQQSVNLMKQLGAEIVDIDLSILK
ECHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEHHHHHHH
YSLAVYYILATAEASTNLARFDGIRYGQRSPKAKTLEEVYDFSKEEGFGAEVKRRILLGT
HHHHHHHHHEECCCCCCHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
YVLSAGYQDAYYKKAQKVRTLILRSYKEAFAKCDLIASPVSPFAAFEIGAIKDPLQMYLE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEECCCCCCHHHHHHH
DIYTIGINLAGLPAVSIPNGFSKDGKPMGLQLIGAQKHDREVLNAAHILEKSLAFHPAIP
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCHH
ELVKEETSA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA