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The map label for this gene is gatB

Identifier: 46446303

GI number: 46446303

Start: 811940

End: 813424

Strand: Reverse

Name: gatB

Synonym: pc0669

Alternate gene names: 46446303

Gene position: 813424-811940 (Counterclockwise)

Preceding gene: 46446304

Following gene: 46446302

Centisome position: 33.69

GC content: 38.25

Gene sequence:

>1485_bases
ATGAGCCATAAATATTCACATCCCTCTCATTCTGACTGGGAAGCAGTGATTGGACTAGAGATCCACGTTGAATTAAATAC
GAAATCTAAATTATTTAGTGTAGCCCCAAATCATTTTGGAGACGAGCCTAACACAAATATTACAGAAGTCTGTACAGGAA
TGCCAGGTTCTTTACCTGTTCTCAATAAAGAAGCTGTTCGCAAAGCGGTCCAATTTGGATGCGCAATTCAAGCCGAAGTT
GCCAAATTTAGTAAATTTGATCGTAAATCTTATTTTTATCCAGATAGTCCCCGTAATTTTCAAATTACCCAATATGATCA
ACCAATTGTTAAAGGGGGAACAGTCATTGCTGAAGTAAATGGAAAGGAAAAATCATTTGCCGTCAACCGTGTTCACCTTG
AAGATGATGCTGGTATGCTTAAGCATTTTTCTACTTTTGCAGGAGTGGATTATAATCGAGCAGGATCTCCTTTAATAGAA
ATTGTGTCTGAACCTTGCATTCATACTCCCGAGGAAGCTGTTGCTTATGCAATGGCTATTAAAGCTATTTTGCAATATAT
CGATGCTTCTGATTGTAACATGGAAGAAGGATCTCTACGAATAGATACAAATATTTCTGTTCGATTAAAAGGAGAACAAG
GCTTAAGAAATAAAATCGAAATTAAAAATATGAATTCGTTCAGTTTCATGGAATTAGCGATTAAATCGGAAATTAATAGA
CAAATTCAAGCTTATCTTAGCCATCCGACAAAACCCCACGATCAAATCATCGCCCAAGCGACTTATCGTTGGGATCCTGA
AAAACAAGAAACCGTTTTAATGCGTCGCAAAGAATCTGCTGACGATTACCGTTATTTTCCCGAGCCCGATTTAGTCCCCA
TCATCTTAACAGACTCCTATATCGAAGAAATTAGACAAAGTCTCCCCGAGCTTCCGTTACAAAGAGAGCGCCGCTATACA
AAAGAGATGGGATTATCTGCTCATCAAGCTTTTGCATTAACTAGCGATAAAGCTCTAGCCGACTATTTTGAAGAAGCCCT
TAAAACTTGTTCTAATAGTAGAAGCCTTTCTAATTGGCTGTTAGTGGAATTTCCTGGCAGACTCAAAGAAGGTGGGCAAA
ATGTGAAGTCCATTAATCTCCCACCATCTCATATCGCTTCTTTAATTAATTTGATTGAAAAAGGGACTATTACAGGGAAA
ATTGCAAAAAGTGTGGCTGACGAAATGGTAGCACAACCTGGCAAAGATCCTGCGGAGATAGTTGCTGGAAATCCTGATTA
TCAGCCATTAAATGATCAAAATGAAGTCGAACGATATGTTGATCAAGTTTTAGCCGAAAATAATCAATCCATTGTCGATT
ATCGAGCTGGGAGAGATAAAGCCTTTGCTTACTTGGTTGGCCAGGTCATGAAGCTTTGTAAAGGTAAAGCTTCTCCTTCA
CTTGTTAATGAACTTCTTAAGCAAAAAATCGCAAATCTCCCTTAA

Upstream 100 bases:

>100_bases
AAAAACATGATAGAGAAGTTTTAAATGCTGCCCATATCTTAGAAAAATCGCTAGCTTTTCATCCTGCTATTCCAGAATTA
GTTAAAGAGGAGACATCTGC

Downstream 100 bases:

>100_bases
CTAATACGAAAAATAGCGGTTTAATTCACGAGTAATCAAGGATTGACTCTTTATTATTTGTTAAGAAGGCTGTATTAATA
TTGTTAATACAGCCTTAGTT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSHKYSHPSHSDWEAVIGLEIHVELNTKSKLFSVAPNHFGDEPNTNITEVCTGMPGSLPVLNKEAVRKAVQFGCAIQAEV
AKFSKFDRKSYFYPDSPRNFQITQYDQPIVKGGTVIAEVNGKEKSFAVNRVHLEDDAGMLKHFSTFAGVDYNRAGSPLIE
IVSEPCIHTPEEAVAYAMAIKAILQYIDASDCNMEEGSLRIDTNISVRLKGEQGLRNKIEIKNMNSFSFMELAIKSEINR
QIQAYLSHPTKPHDQIIAQATYRWDPEKQETVLMRRKESADDYRYFPEPDLVPIILTDSYIEEIRQSLPELPLQRERRYT
KEMGLSAHQAFALTSDKALADYFEEALKTCSNSRSLSNWLLVEFPGRLKEGGQNVKSINLPPSHIASLINLIEKGTITGK
IAKSVADEMVAQPGKDPAEIVAGNPDYQPLNDQNEVERYVDQVLAENNQSIVDYRAGRDKAFAYLVGQVMKLCKGKASPS
LVNELLKQKIANLP

Sequences:

>Translated_494_residues
MSHKYSHPSHSDWEAVIGLEIHVELNTKSKLFSVAPNHFGDEPNTNITEVCTGMPGSLPVLNKEAVRKAVQFGCAIQAEV
AKFSKFDRKSYFYPDSPRNFQITQYDQPIVKGGTVIAEVNGKEKSFAVNRVHLEDDAGMLKHFSTFAGVDYNRAGSPLIE
IVSEPCIHTPEEAVAYAMAIKAILQYIDASDCNMEEGSLRIDTNISVRLKGEQGLRNKIEIKNMNSFSFMELAIKSEINR
QIQAYLSHPTKPHDQIIAQATYRWDPEKQETVLMRRKESADDYRYFPEPDLVPIILTDSYIEEIRQSLPELPLQRERRYT
KEMGLSAHQAFALTSDKALADYFEEALKTCSNSRSLSNWLLVEFPGRLKEGGQNVKSINLPPSHIASLINLIEKGTITGK
IAKSVADEMVAQPGKDPAEIVAGNPDYQPLNDQNEVERYVDQVLAENNQSIVDYRAGRDKAFAYLVGQVMKLCKGKASPS
LVNELLKQKIANLP
>Mature_493_residues
SHKYSHPSHSDWEAVIGLEIHVELNTKSKLFSVAPNHFGDEPNTNITEVCTGMPGSLPVLNKEAVRKAVQFGCAIQAEVA
KFSKFDRKSYFYPDSPRNFQITQYDQPIVKGGTVIAEVNGKEKSFAVNRVHLEDDAGMLKHFSTFAGVDYNRAGSPLIEI
VSEPCIHTPEEAVAYAMAIKAILQYIDASDCNMEEGSLRIDTNISVRLKGEQGLRNKIEIKNMNSFSFMELAIKSEINRQ
IQAYLSHPTKPHDQIIAQATYRWDPEKQETVLMRRKESADDYRYFPEPDLVPIILTDSYIEEIRQSLPELPLQRERRYTK
EMGLSAHQAFALTSDKALADYFEEALKTCSNSRSLSNWLLVEFPGRLKEGGQNVKSINLPPSHIASLINLIEKGTITGKI
AKSVADEMVAQPGKDPAEIVAGNPDYQPLNDQNEVERYVDQVLAENNQSIVDYRAGRDKAFAYLVGQVMKLCKGKASPSL
VNELLKQKIANLP

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=502, Percent_Identity=34.0637450199203, Blast_Score=266, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI115534094, Length=350, Percent_Identity=32.2857142857143, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=27.6923076923077, Blast_Score=180, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=30.0403225806452, Blast_Score=229, Evalue=4e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PARUW (Q6MDF6)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007668.1
- ProteinModelPortal:   Q6MDF6
- SMR:   Q6MDF6
- STRING:   Q6MDF6
- GeneID:   2780576
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0669
- NMPDR:   fig|264201.1.peg.669
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CPRO264201:PC0669-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 55379; Mature: 55248

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKYSHPSHSDWEAVIGLEIHVELNTKSKLFSVAPNHFGDEPNTNITEVCTGMPGSLPV
CCCCCCCCCCCCCEEEEEEEEEEEECCCCHHEEECCHHCCCCCCCCHHHHHCCCCCCCCC
LNKEAVRKAVQFGCAIQAEVAKFSKFDRKSYFYPDSPRNFQITQYDQPIVKGGTVIAEVN
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHCCCEEEEEEC
GKEKSFAVNRVHLEDDAGMLKHFSTFAGVDYNRAGSPLIEIVSEPCIHTPEEAVAYAMAI
CCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
KAILQYIDASDCNMEEGSLRIDTNISVRLKGEQGLRNKIEIKNMNSFSFMELAIKSEINR
HHHHHHHCCCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
QIQAYLSHPTKPHDQIIAQATYRWDPEKQETVLMRRKESADDYRYFPEPDLVPIILTDSY
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHH
IEEIRQSLPELPLQRERRYTKEMGLSAHQAFALTSDKALADYFEEALKTCSNSRSLSNWL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCEE
LVEFPGRLKEGGQNVKSINLPPSHIASLINLIEKGTITGKIAKSVADEMVAQPGKDPAEI
EEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHE
VAGNPDYQPLNDQNEVERYVDQVLAENNQSIVDYRAGRDKAFAYLVGQVMKLCKGKASPS
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
LVNELLKQKIANLP
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SHKYSHPSHSDWEAVIGLEIHVELNTKSKLFSVAPNHFGDEPNTNITEVCTGMPGSLPV
CCCCCCCCCCCCEEEEEEEEEEEECCCCHHEEECCHHCCCCCCCCHHHHHCCCCCCCCC
LNKEAVRKAVQFGCAIQAEVAKFSKFDRKSYFYPDSPRNFQITQYDQPIVKGGTVIAEVN
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHCCCEEEEEEC
GKEKSFAVNRVHLEDDAGMLKHFSTFAGVDYNRAGSPLIEIVSEPCIHTPEEAVAYAMAI
CCCCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHH
KAILQYIDASDCNMEEGSLRIDTNISVRLKGEQGLRNKIEIKNMNSFSFMELAIKSEINR
HHHHHHHCCCCCCCCCCCEEEECCEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
QIQAYLSHPTKPHDQIIAQATYRWDPEKQETVLMRRKESADDYRYFPEPDLVPIILTDSY
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHH
IEEIRQSLPELPLQRERRYTKEMGLSAHQAFALTSDKALADYFEEALKTCSNSRSLSNWL
HHHHHHHCCCCCHHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHHHCCCCCCCCEE
LVEFPGRLKEGGQNVKSINLPPSHIASLINLIEKGTITGKIAKSVADEMVAQPGKDPAEI
EEECCCHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHE
VAGNPDYQPLNDQNEVERYVDQVLAENNQSIVDYRAGRDKAFAYLVGQVMKLCKGKASPS
ECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHH
LVNELLKQKIANLP
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA