Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 46446078

GI number: 46446078

Start: 572695

End: 574170

Strand: Reverse

Name: glyA [H]

Synonym: pc0444

Alternate gene names: 46446078

Gene position: 574170-572695 (Counterclockwise)

Preceding gene: 46446079

Following gene: 46446077

Centisome position: 23.78

GC content: 37.6

Gene sequence:

>1476_bases
ATGTCAAGACTTAAAAAATATCTCTCTTCTAAAACAAGCAATCAACAAAATTCAGCAGCGATTGCTTATTTAGCCGCTCT
TGATCATATTGAAACTGTCTCCCCTACTATTTCTTCTAGTATTATTCAAGAATTACAAGATGAACGCTCTCATCTAAAAC
TTATTGCATCTGAAAACTTTTCGTCTTTGGCTGTTCAGTTAGCAATGGGTAACCTTTTAACTGACAAGTATGCTGAAGGA
TATGCACATCACCGATTTTATGCGGGATGCGATAACATAGATAGCATTGAAGAAACAGCTAGTCAAGAATTGATTCAATT
ATTTGGATGTGAGCATGCCTATGTTCAACCCCATTCAGGAGCTGACGCAAATTTAGTGGCACTATGGGCCATCTTAATCC
ATAAAATTCAAAATCCGGAAATTGAAAAATTTGGAAAAAAAACTTTAGATGAGTTAACTCCCGAAGAATATGAAAAAATT
CGGCAACTTCTCGTTAATCAAAAACTCATGGGCATGTCCTTAAATTCCGGGGGGCATTTAACGCATGGATATCGCCATAA
TATCTCATCTAAAATGATGCGTTCTGTCTTATATGATGTTGATCCCAAAACCGAAATTCTTGATTACTCTGTACTTGCAA
AACAAGTGCAACAAGAAAGACCGACAATTCTTATTGCTGGTTATTCGGCTCATCCAAGGCGACTTAATTTCGCTAAAATG
CGTGAAATTGCAGATAGTGTAGGAGCTACTTTGATGGTAGATATGGCCCACTTTGCCGGTCTGGTAGCCGGAAAAGTTTT
CCAAGGCGAATTCGATCCTATTCCTTATGCAGATATTGTGACTTCTACTACGCATAAAACATTGAGAGGCCCAAGAGGAG
GTTTTGTTTTATGTAAACAGTCCTTTAGCGAAGCCATTAATAAAGGTTGTCCGTCAGTTCTTGGAGGGCCACTTCCCAAC
GTTATGGCAGCAAAAGCCGTCGCTTTTAAAGAAGCAAACTCCCTTAATTTTAAACAGTACTCACAAAAAATTGTTGATAA
TGCTCAATCCTTAGCCAATTGCTTTCTTCAAAATGACATTCGTCTTGTTACCGGTGGAACAGAAAACCATTTAATGATTC
TCGATTTATCTAAATTTGGATTAACCGGTCGTCAAGCAGAAACTGCATTAAGAGAAGCCCATATCACAGTTAACAGAAAT
GCAATTCCAAATGATCTTCAAGGGCCTTGGTACACATCTGGAATTCGTTTAGGAACAGCAGCTTTAACAACCTTGGGAAT
GGGTAAAGATGAAATGAATGAAATTGCTTCTGTTATTTTCTCTGTCCTATCAAACTCTAAACCTGGCATTGTTCAAAAAA
CTGGACAACCAAGCAAGGCTTCAGTCATCACAGATTCCACAATTATCAATGAAGCAAAGGCACGAGTAAAAAATTTATTA
ATTCATTTCCCTCTCTATCCTGAAATAGAAATTTAA

Upstream 100 bases:

>100_bases
ACTCACGGTAGTCAACGAAAAATAAATGCAAAAGATGTGGATTTAACGAGTAACTGCATGAAACTCAACAGATTTTCAGT
CGTCATAAGGAGAACAGCAT

Downstream 100 bases:

>100_bases
TAAACTTTACTATCAAATCGATTTAGAGTTTGACACAATCAAACATTTTTTCTTATTTTGCGCCTTTAGAACTTGAAAAA
GGATATCAGAAATCATAAGA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSRLKKYLSSKTSNQQNSAAIAYLAALDHIETVSPTISSSIIQELQDERSHLKLIASENFSSLAVQLAMGNLLTDKYAEG
YAHHRFYAGCDNIDSIEETASQELIQLFGCEHAYVQPHSGADANLVALWAILIHKIQNPEIEKFGKKTLDELTPEEYEKI
RQLLVNQKLMGMSLNSGGHLTHGYRHNISSKMMRSVLYDVDPKTEILDYSVLAKQVQQERPTILIAGYSAHPRRLNFAKM
REIADSVGATLMVDMAHFAGLVAGKVFQGEFDPIPYADIVTSTTHKTLRGPRGGFVLCKQSFSEAINKGCPSVLGGPLPN
VMAAKAVAFKEANSLNFKQYSQKIVDNAQSLANCFLQNDIRLVTGGTENHLMILDLSKFGLTGRQAETALREAHITVNRN
AIPNDLQGPWYTSGIRLGTAALTTLGMGKDEMNEIASVIFSVLSNSKPGIVQKTGQPSKASVITDSTIINEAKARVKNLL
IHFPLYPEIEI

Sequences:

>Translated_491_residues
MSRLKKYLSSKTSNQQNSAAIAYLAALDHIETVSPTISSSIIQELQDERSHLKLIASENFSSLAVQLAMGNLLTDKYAEG
YAHHRFYAGCDNIDSIEETASQELIQLFGCEHAYVQPHSGADANLVALWAILIHKIQNPEIEKFGKKTLDELTPEEYEKI
RQLLVNQKLMGMSLNSGGHLTHGYRHNISSKMMRSVLYDVDPKTEILDYSVLAKQVQQERPTILIAGYSAHPRRLNFAKM
REIADSVGATLMVDMAHFAGLVAGKVFQGEFDPIPYADIVTSTTHKTLRGPRGGFVLCKQSFSEAINKGCPSVLGGPLPN
VMAAKAVAFKEANSLNFKQYSQKIVDNAQSLANCFLQNDIRLVTGGTENHLMILDLSKFGLTGRQAETALREAHITVNRN
AIPNDLQGPWYTSGIRLGTAALTTLGMGKDEMNEIASVIFSVLSNSKPGIVQKTGQPSKASVITDSTIINEAKARVKNLL
IHFPLYPEIEI
>Mature_490_residues
SRLKKYLSSKTSNQQNSAAIAYLAALDHIETVSPTISSSIIQELQDERSHLKLIASENFSSLAVQLAMGNLLTDKYAEGY
AHHRFYAGCDNIDSIEETASQELIQLFGCEHAYVQPHSGADANLVALWAILIHKIQNPEIEKFGKKTLDELTPEEYEKIR
QLLVNQKLMGMSLNSGGHLTHGYRHNISSKMMRSVLYDVDPKTEILDYSVLAKQVQQERPTILIAGYSAHPRRLNFAKMR
EIADSVGATLMVDMAHFAGLVAGKVFQGEFDPIPYADIVTSTTHKTLRGPRGGFVLCKQSFSEAINKGCPSVLGGPLPNV
MAAKAVAFKEANSLNFKQYSQKIVDNAQSLANCFLQNDIRLVTGGTENHLMILDLSKFGLTGRQAETALREAHITVNRNA
IPNDLQGPWYTSGIRLGTAALTTLGMGKDEMNEIASVIFSVLSNSKPGIVQKTGQPSKASVITDSTIINEAKARVKNLLI
HFPLYPEIEI

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=425, Percent_Identity=36.4705882352941, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI261862352, Length=425, Percent_Identity=36.4705882352941, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI261862350, Length=425, Percent_Identity=36.4705882352941, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI261862348, Length=425, Percent_Identity=36.4705882352941, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI261862346, Length=421, Percent_Identity=35.6294536817102, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI22547186, Length=424, Percent_Identity=34.4339622641509, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI22547189, Length=408, Percent_Identity=34.0686274509804, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1788902, Length=448, Percent_Identity=40.625, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25144732, Length=437, Percent_Identity=35.0114416475973, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25144729, Length=437, Percent_Identity=35.0114416475973, Blast_Score=263, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319739, Length=434, Percent_Identity=36.1751152073733, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323087, Length=492, Percent_Identity=33.739837398374, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24640005, Length=464, Percent_Identity=36.4224137931034, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI221329721, Length=433, Percent_Identity=37.4133949191686, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 53971; Mature: 53840

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKKYLSSKTSNQQNSAAIAYLAALDHIETVSPTISSSIIQELQDERSHLKLIASENF
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEHHCCCH
SSLAVQLAMGNLLTDKYAEGYAHHRFYAGCDNIDSIEETASQELIQLFGCEHAYVQPHSG
HHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
ADANLVALWAILIHKIQNPEIEKFGKKTLDELTPEEYEKIRQLLVNQKLMGMSLNSGGHL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCEEECCCCCC
THGYRHNISSKMMRSVLYDVDPKTEILDYSVLAKQVQQERPTILIAGYSAHPRRLNFAKM
CCCHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
REIADSVGATLMVDMAHFAGLVAGKVFQGEFDPIPYADIVTSTTHKTLRGPRGGFVLCKQ
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHH
SFSEAINKGCPSVLGGPLPNVMAAKAVAFKEANSLNFKQYSQKIVDNAQSLANCFLQNDI
HHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RLVTGGTENHLMILDLSKFGLTGRQAETALREAHITVNRNAIPNDLQGPWYTSGIRLGTA
EEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEHHHH
ALTTLGMGKDEMNEIASVIFSVLSNSKPGIVQKTGQPSKASVITDSTIINEAKARVKNLL
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHE
IHFPLYPEIEI
EECCCCCCCCC
>Mature Secondary Structure 
SRLKKYLSSKTSNQQNSAAIAYLAALDHIETVSPTISSSIIQELQDERSHLKLIASENF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEHHCCCH
SSLAVQLAMGNLLTDKYAEGYAHHRFYAGCDNIDSIEETASQELIQLFGCEHAYVQPHSG
HHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCC
ADANLVALWAILIHKIQNPEIEKFGKKTLDELTPEEYEKIRQLLVNQKLMGMSLNSGGHL
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCEEECCCCCC
THGYRHNISSKMMRSVLYDVDPKTEILDYSVLAKQVQQERPTILIAGYSAHPRRLNFAKM
CCCHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
REIADSVGATLMVDMAHFAGLVAGKVFQGEFDPIPYADIVTSTTHKTLRGPRGGFVLCKQ
HHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHH
SFSEAINKGCPSVLGGPLPNVMAAKAVAFKEANSLNFKQYSQKIVDNAQSLANCFLQNDI
HHHHHHHCCCCHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
RLVTGGTENHLMILDLSKFGLTGRQAETALREAHITVNRNAIPNDLQGPWYTSGIRLGTA
EEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCEEHHHH
ALTTLGMGKDEMNEIASVIFSVLSNSKPGIVQKTGQPSKASVITDSTIINEAKARVKNLL
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHHHHHE
IHFPLYPEIEI
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]