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The map label for this gene is dac [H]

Identifier: 46446028

GI number: 46446028

Start: 526665

End: 528035

Strand: Direct

Name: dac [H]

Synonym: pc0394

Alternate gene names: 46446028

Gene position: 526665-528035 (Clockwise)

Preceding gene: 46446026

Following gene: 46446032

Centisome position: 21.81

GC content: 36.18

Gene sequence:

>1371_bases
TTGATTTTCAATTTTTTAGTTGTTTTTTTTTATAATTTCTCGGATTTTATTCGTAATATTTTAGTAAGTAAGCGTCAAGT
TGAACATGGGTTTAAAATGAGAGTCTGGTACATTTTTTTTGTGACAATAATTTCTTTTTCCTTACGATCAGCCCCCCTTG
ATTTTAAAATTGCTGGGGAAGCGGCTATTTTACTTAATGCTGAAAGTGGAGCAATTCTATTTGAACAAGAAGCCTACTCT
TGCCAATTTCCAGCCAGTACAACAAAAGTAGCCACAGCTTTATATGCGTTAAGTAAAAAGCAAGATCTTAACCAGATGAT
GGCCGCTGAGCAAGACTCACTGACGACACTTTCGCAAGAAGCTAAGAAGAAAACTAATTATGATCCTCCTTATCGACTAG
AACCTGATGGCAGTCACATAGGTCTAAAAAAAGGAGAAATGATGTCATTGCACGATCTTTTAGGAGGGATGTTAATTCCT
TCTGGAAATGATGCCGCAAACGTAATTGCTAATGCCCTAGGCCCTACAATTCCAGTTTTTATGGCAGAGTTGAACATTTA
CTTAAAAAAAATTGGCTGTGAGAAAACAACTTTTTATAATCCTCATGGTCTGCATCATCCTCAACATCAAACAACGGCTT
ACGATTTAGCTCTAATAACCAAAGAAGCTTTGAAACAACCTGTTTTTTGCGAATTGATTGGACAAAAAAAATATTTAAGG
CCAAAAACGAATAAACAAGCGGCAACGACTTTATTACAGACAAATCGGTTAATTCGCCCCGGAAGCTTTTATTATTCTAA
AGCGATTGGAGGAAAAACTGGTTATCATGCAAAAGCTAAAAAAACATATATTGGAGTCGCTCGATCGGAAGAAAGGACCT
TGATAGTGGTTTTATTGGGTTATCAAGAGAGAAATACCATTTTTCAAGATGCTATTAAATTGTTTGATGCTGCTTTCAAT
CAACCTAAAATTCAACGTACATTTTTGAAAGAAGGAAGACAGTCATTTGCTCATAATCTTCCTAAAGCTGCTTGCCCGCT
ACAAACGCATTTAAAAGAACCATTGAAGTTAGACTATTATCTGGCAGAAGATCCCGAAGCTAAATGTCTTTTATATTGGA
TGATTCCACCTTTACCTATCCAAAAAGATCAACAAGTGGGAGAGTTACATCTCATTGCTAAGAATGGTAGCTTAATTAAA
AAGGCCTCTCTTTTAGCATTAGGAGATGTTTCTTACGCATGGCCCTATAGCTGGATGGCTAATATCGAGGGTTTTTTCGG
TCAACTTTCTTGGCTGTGGGTAAGTTTGATTAGCCTATTTATGTTTGGGTTATTGTTTTTCATTTGGAAAACTAGTCCAA
AAGAGCTCTAA

Upstream 100 bases:

>100_bases
ATAAAAACGTATTGGCTAATTTCATTCAATTAGTAAATTCATTCACTTCTAGAGGTCTGAAAATACTAATCTTACATAAT
AATTTTAATTTATTTTTTAA

Downstream 100 bases:

>100_bases
AATATACTCAACTCTCAACCTTAATTGAAATAAAATTTCTTTTAAAATTGATTGTCATTAAAGCAAGAAAGCATTTGTGA
AAATAAATTTCACCATTAAA

Product: putative serine-type D-Ala-D-Ala carboxypeptidase (penicillin binding protein)

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIFNFLVVFFYNFSDFIRNILVSKRQVEHGFKMRVWYIFFVTIISFSLRSAPLDFKIAGEAAILLNAESGAILFEQEAYS
CQFPASTTKVATALYALSKKQDLNQMMAAEQDSLTTLSQEAKKKTNYDPPYRLEPDGSHIGLKKGEMMSLHDLLGGMLIP
SGNDAANVIANALGPTIPVFMAELNIYLKKIGCEKTTFYNPHGLHHPQHQTTAYDLALITKEALKQPVFCELIGQKKYLR
PKTNKQAATTLLQTNRLIRPGSFYYSKAIGGKTGYHAKAKKTYIGVARSEERTLIVVLLGYQERNTIFQDAIKLFDAAFN
QPKIQRTFLKEGRQSFAHNLPKAACPLQTHLKEPLKLDYYLAEDPEAKCLLYWMIPPLPIQKDQQVGELHLIAKNGSLIK
KASLLALGDVSYAWPYSWMANIEGFFGQLSWLWVSLISLFMFGLLFFIWKTSPKEL

Sequences:

>Translated_456_residues
MIFNFLVVFFYNFSDFIRNILVSKRQVEHGFKMRVWYIFFVTIISFSLRSAPLDFKIAGEAAILLNAESGAILFEQEAYS
CQFPASTTKVATALYALSKKQDLNQMMAAEQDSLTTLSQEAKKKTNYDPPYRLEPDGSHIGLKKGEMMSLHDLLGGMLIP
SGNDAANVIANALGPTIPVFMAELNIYLKKIGCEKTTFYNPHGLHHPQHQTTAYDLALITKEALKQPVFCELIGQKKYLR
PKTNKQAATTLLQTNRLIRPGSFYYSKAIGGKTGYHAKAKKTYIGVARSEERTLIVVLLGYQERNTIFQDAIKLFDAAFN
QPKIQRTFLKEGRQSFAHNLPKAACPLQTHLKEPLKLDYYLAEDPEAKCLLYWMIPPLPIQKDQQVGELHLIAKNGSLIK
KASLLALGDVSYAWPYSWMANIEGFFGQLSWLWVSLISLFMFGLLFFIWKTSPKEL
>Mature_456_residues
MIFNFLVVFFYNFSDFIRNILVSKRQVEHGFKMRVWYIFFVTIISFSLRSAPLDFKIAGEAAILLNAESGAILFEQEAYS
CQFPASTTKVATALYALSKKQDLNQMMAAEQDSLTTLSQEAKKKTNYDPPYRLEPDGSHIGLKKGEMMSLHDLLGGMLIP
SGNDAANVIANALGPTIPVFMAELNIYLKKIGCEKTTFYNPHGLHHPQHQTTAYDLALITKEALKQPVFCELIGQKKYLR
PKTNKQAATTLLQTNRLIRPGSFYYSKAIGGKTGYHAKAKKTYIGVARSEERTLIVVLLGYQERNTIFQDAIKLFDAAFN
QPKIQRTFLKEGRQSFAHNLPKAACPLQTHLKEPLKLDYYLAEDPEAKCLLYWMIPPLPIQKDQQVGELHLIAKNGSLIK
KASLLALGDVSYAWPYSWMANIEGFFGQLSWLWVSLISLFMFGLLFFIWKTSPKEL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=417, Percent_Identity=25.6594724220624, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082022, Length=353, Percent_Identity=26.0623229461756, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786851, Length=249, Percent_Identity=26.5060240963855, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 51638; Mature: 51638

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNFLVVFFYNFSDFIRNILVSKRQVEHGFKMRVWYIFFVTIISFSLRSAPLDFKIAGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
AAILLNAESGAILFEQEAYSCQFPASTTKVATALYALSKKQDLNQMMAAEQDSLTTLSQE
EEEEEECCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AKKKTNYDPPYRLEPDGSHIGLKKGEMMSLHDLLGGMLIPSGNDAANVIANALGPTIPVF
HHHCCCCCCCEEECCCCCEECCCCCCHHHHHHHHCCEECCCCCCHHHHHHHHHCCHHHHH
MAELNIYLKKIGCEKTTFYNPHGLHHPQHQTTAYDLALITKEALKQPVFCELIGQKKYLR
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
PKTNKQAATTLLQTNRLIRPGSFYYSKAIGGKTGYHAKAKKTYIGVARSEERTLIVVLLG
CCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEC
YQERNTIFQDAIKLFDAAFNQPKIQRTFLKEGRQSFAHNLPKAACPLQTHLKEPLKLDYY
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEE
LAEDPEAKCLLYWMIPPLPIQKDQQVGELHLIAKNGSLIKKASLLALGDVSYAWPYSWMA
ECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHEEEECCCCCCCCHHHHH
NIEGFFGQLSWLWVSLISLFMFGLLFFIWKTSPKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFNFLVVFFYNFSDFIRNILVSKRQVEHGFKMRVWYIFFVTIISFSLRSAPLDFKIAGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECC
AAILLNAESGAILFEQEAYSCQFPASTTKVATALYALSKKQDLNQMMAAEQDSLTTLSQE
EEEEEECCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AKKKTNYDPPYRLEPDGSHIGLKKGEMMSLHDLLGGMLIPSGNDAANVIANALGPTIPVF
HHHCCCCCCCEEECCCCCEECCCCCCHHHHHHHHCCEECCCCCCHHHHHHHHHCCHHHHH
MAELNIYLKKIGCEKTTFYNPHGLHHPQHQTTAYDLALITKEALKQPVFCELIGQKKYLR
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
PKTNKQAATTLLQTNRLIRPGSFYYSKAIGGKTGYHAKAKKTYIGVARSEERTLIVVLLG
CCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEC
YQERNTIFQDAIKLFDAAFNQPKIQRTFLKEGRQSFAHNLPKAACPLQTHLKEPLKLDYY
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEE
LAEDPEAKCLLYWMIPPLPIQKDQQVGELHLIAKNGSLIKKASLLALGDVSYAWPYSWMA
ECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCEEHHHHEEEECCCCCCCCHHHHH
NIEGFFGQLSWLWVSLISLFMFGLLFFIWKTSPKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]