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The map label for this gene is ddlA [H]

Identifier: 46446032

GI number: 46446032

Start: 532019

End: 534415

Strand: Direct

Name: ddlA [H]

Synonym: pc0398

Alternate gene names: 46446032

Gene position: 532019-534415 (Clockwise)

Preceding gene: 46446028

Following gene: 46446033

Centisome position: 22.03

GC content: 34.04

Gene sequence:

>2397_bases
ATGTTTAAAATAGCTCTTATCTGCGGAGGTCCTTCATTAGAGAGAGGGATATCTCTGAATTCTGCTCGCTCTGTTCTAGA
TCATTTGGCTTCTGAGTTTATAGAGATTATTCCTATTTACGTAGACTATCAAAATTTTTTTTATCTTATTTCAACAGCTC
AACTTTATTCAAATACCCCGGCGGATTTTGATTTTAAATTAAAAAAGTCAGCGCAAGCAATGAATCGAGAAGAATTTAAA
AAAATTTTAAGAAATATAGATTTGGTTTTTCCTGTTATTCATGGAATATTTGGTGAAGATGGAGAATTACAAAAAATTTT
GGAAGAAATAGATGTTCCTTTTGTAGGAACAGGTAGTCAAGCCTGTGCAGAAATGTTTTGTAAATATCATGCTTCGCAAA
TCCTACATCAAAATCGATTTCCGGCTTTTATGAAATTGGAAGTGTGCCCTGAAACCAATGATTTGGCACCTATTAAATCT
TTTTTTGAACAGCATCAGTTAAAAAGAGCTATTGTTAAACCAACCATGGGTGGATCTTCGATAGGAGTGACCTCTGTTCA
GACTCCAGAAGATGCTTTTGAAAAAATTAAAATGCTTTGCCAGCAAGGAATAGCTAAACGAGCCCTAATAGAGCCTTTTT
GTGAAGGGACAGAATTTACCGTTGTTGTCCTTCAAAATCAACAAAGTAAACCTGTCGCTTTAATCCCGACTGAAATTGAA
ATGAGTTATCAAAACCATCAGATTTTTGATTATCGAAAAAAATATCTTCCTACAAACCAAACAACTTATCATACCCCTGC
ACGCTTTGATGAGACGATTATTCAAGGCATTAGACAAAAAGCCGAAGCAATTTTTACATTATTTCATATGCGCGATTTCG
CTCGTTTAGATGGTTGGTTACTAAATGATGGAACAATTTATTTTACTGACCTTAATCCAATTAGTGGAATGGAGCAGAAC
AGTTTTCTTTTTAGGCAAGCCTCTCTTCTTGGATTAACTCACCGCGATGTGTTTCGTTTGATCATTACAAGTGCGTGTTT
GAGATGGAAATTGGCTCCTCCAACCTTTGTTGATGAATCGATGCTAGAAAAAAAAAAGGTTCATGTTCTGTTTGGAGGCA
AAAATGCAGAAAGACAAGTTTCATTAATGAGTGGAACAAATGTTTGGTTAAAGCTTTTACGATCATCTAAGTATCATCCT
ATCCCTTTTTTCTATGGAAAAAATGACTTAATCTGGCAACTACCTTATGCTTATACACTGGATCATACAGTTGAAGAAAT
TTTACATAGGTGTTTAGCTAGAGAAGCATCGAACAAAAAACTTCCGAACTATTTTAATTCTATTTGCGAGCAGCTTGGTA
TTCCATATAATTTTCAAGAAACTCCTTCTAGCATGTGTCTACAAGATTTTTATCAACTTTCTGCCTCTAACCAAGCCTTT
GTTTTTATTGCTATGCATGGAGGGGAAGGGGAAAATGGAATTTTGCAAAAAGAATTGGAAAAAAACCAACTGTATTATAA
TGGCTCTTGTGCAAAAACTTCTTCCATTTGTATGGATAAATTTTTAACAGGTCAAGTAATTGAACAGCTCAAACATCCAA
ATATATTTTCGCTTCCTAAAGAATGTGTAGAAGCTGCCAATTTGTTCGGTCAATCTACAAAATACTTTTTGGAAAAATGG
AATAAAATTTGTATTTCATTAAGATCGAATCGACTAATTATTAAACCACGTCAAGATGGATGTTCCGCCGGAATTGTTTT
ATTAGAATCTGGGGCAGATTTAGAAAAGTATTTTGCGTTTATTCAAAATAACGTTTCGTTAATTCCAAAAGGTTCTTTTA
GCCAACAAAAAGACGTGATTGAAATGCCTTCATCAACTTGCGTTGAATACTTATTAGAACCTTATATCGAAACAGATCGA
ATTATCCTTGATCAAGGAGAGCTAATTCATGAAATAAAAGAAGGGTGGGTAGAATTGACTGTCGGGGTTCTTGAGCATAA
TCAAATTTATCGCTCATTGAATCCAAGCATTACACTTGCTGAAGGAGCCGTTTTAAGTTTGGAAGAAAAATTTCAAGGAG
GGACGGGAGTTAATTTAACCCCACCTCCAGAAAAAATTCTAGCTTCTGAAGATGTTTCTCAAATTAAATTTTTAATTGAG
AAAACGGCAGATGCTTTGCAAATTCAAAATTATGCTCGACTTGATATTTTTTATCATCGAGAGACAAAAAAAATGGTTGT
TATTGAAGCTAATACACTTCCAGGGCTCACTCCATCGACAGTTTTTTATCAACAAGGTTTAGCTGAAAAAAAACCTTTGA
ATCCTTTAAGCCTTTTAGAAATCATAATTAGTTCAAAATTAGAGTTGAACAAAGAAAATATAATATTCAACGATTAA

Upstream 100 bases:

>100_bases
ATTTCTTTAAAAATGGTTTCTAGAATAATTCGTCATCGACTAATTATATTAAGTTTATTACACTTATTCTTCACATTCTT
GACTATCAGGTTGAAGATTC

Downstream 100 bases:

>100_bases
AAAATTGTTTAGATAAATAATTCTTTGTTAACTTTTTCTTAATAAGATTTTTATTTATTTTTAATTTCCTTGTAATAAGA
TTTGCAGAAATAAGTATAAT

Product: putative D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MFKIALICGGPSLERGISLNSARSVLDHLASEFIEIIPIYVDYQNFFYLISTAQLYSNTPADFDFKLKKSAQAMNREEFK
KILRNIDLVFPVIHGIFGEDGELQKILEEIDVPFVGTGSQACAEMFCKYHASQILHQNRFPAFMKLEVCPETNDLAPIKS
FFEQHQLKRAIVKPTMGGSSIGVTSVQTPEDAFEKIKMLCQQGIAKRALIEPFCEGTEFTVVVLQNQQSKPVALIPTEIE
MSYQNHQIFDYRKKYLPTNQTTYHTPARFDETIIQGIRQKAEAIFTLFHMRDFARLDGWLLNDGTIYFTDLNPISGMEQN
SFLFRQASLLGLTHRDVFRLIITSACLRWKLAPPTFVDESMLEKKKVHVLFGGKNAERQVSLMSGTNVWLKLLRSSKYHP
IPFFYGKNDLIWQLPYAYTLDHTVEEILHRCLAREASNKKLPNYFNSICEQLGIPYNFQETPSSMCLQDFYQLSASNQAF
VFIAMHGGEGENGILQKELEKNQLYYNGSCAKTSSICMDKFLTGQVIEQLKHPNIFSLPKECVEAANLFGQSTKYFLEKW
NKICISLRSNRLIIKPRQDGCSAGIVLLESGADLEKYFAFIQNNVSLIPKGSFSQQKDVIEMPSSTCVEYLLEPYIETDR
IILDQGELIHEIKEGWVELTVGVLEHNQIYRSLNPSITLAEGAVLSLEEKFQGGTGVNLTPPPEKILASEDVSQIKFLIE
KTADALQIQNYARLDIFYHRETKKMVVIEANTLPGLTPSTVFYQQGLAEKKPLNPLSLLEIIISSKLELNKENIIFND

Sequences:

>Translated_798_residues
MFKIALICGGPSLERGISLNSARSVLDHLASEFIEIIPIYVDYQNFFYLISTAQLYSNTPADFDFKLKKSAQAMNREEFK
KILRNIDLVFPVIHGIFGEDGELQKILEEIDVPFVGTGSQACAEMFCKYHASQILHQNRFPAFMKLEVCPETNDLAPIKS
FFEQHQLKRAIVKPTMGGSSIGVTSVQTPEDAFEKIKMLCQQGIAKRALIEPFCEGTEFTVVVLQNQQSKPVALIPTEIE
MSYQNHQIFDYRKKYLPTNQTTYHTPARFDETIIQGIRQKAEAIFTLFHMRDFARLDGWLLNDGTIYFTDLNPISGMEQN
SFLFRQASLLGLTHRDVFRLIITSACLRWKLAPPTFVDESMLEKKKVHVLFGGKNAERQVSLMSGTNVWLKLLRSSKYHP
IPFFYGKNDLIWQLPYAYTLDHTVEEILHRCLAREASNKKLPNYFNSICEQLGIPYNFQETPSSMCLQDFYQLSASNQAF
VFIAMHGGEGENGILQKELEKNQLYYNGSCAKTSSICMDKFLTGQVIEQLKHPNIFSLPKECVEAANLFGQSTKYFLEKW
NKICISLRSNRLIIKPRQDGCSAGIVLLESGADLEKYFAFIQNNVSLIPKGSFSQQKDVIEMPSSTCVEYLLEPYIETDR
IILDQGELIHEIKEGWVELTVGVLEHNQIYRSLNPSITLAEGAVLSLEEKFQGGTGVNLTPPPEKILASEDVSQIKFLIE
KTADALQIQNYARLDIFYHRETKKMVVIEANTLPGLTPSTVFYQQGLAEKKPLNPLSLLEIIISSKLELNKENIIFND
>Mature_798_residues
MFKIALICGGPSLERGISLNSARSVLDHLASEFIEIIPIYVDYQNFFYLISTAQLYSNTPADFDFKLKKSAQAMNREEFK
KILRNIDLVFPVIHGIFGEDGELQKILEEIDVPFVGTGSQACAEMFCKYHASQILHQNRFPAFMKLEVCPETNDLAPIKS
FFEQHQLKRAIVKPTMGGSSIGVTSVQTPEDAFEKIKMLCQQGIAKRALIEPFCEGTEFTVVVLQNQQSKPVALIPTEIE
MSYQNHQIFDYRKKYLPTNQTTYHTPARFDETIIQGIRQKAEAIFTLFHMRDFARLDGWLLNDGTIYFTDLNPISGMEQN
SFLFRQASLLGLTHRDVFRLIITSACLRWKLAPPTFVDESMLEKKKVHVLFGGKNAERQVSLMSGTNVWLKLLRSSKYHP
IPFFYGKNDLIWQLPYAYTLDHTVEEILHRCLAREASNKKLPNYFNSICEQLGIPYNFQETPSSMCLQDFYQLSASNQAF
VFIAMHGGEGENGILQKELEKNQLYYNGSCAKTSSICMDKFLTGQVIEQLKHPNIFSLPKECVEAANLFGQSTKYFLEKW
NKICISLRSNRLIIKPRQDGCSAGIVLLESGADLEKYFAFIQNNVSLIPKGSFSQQKDVIEMPSSTCVEYLLEPYIETDR
IILDQGELIHEIKEGWVELTVGVLEHNQIYRSLNPSITLAEGAVLSLEEKFQGGTGVNLTPPPEKILASEDVSQIKFLIE
KTADALQIQNYARLDIFYHRETKKMVVIEANTLPGLTPSTVFYQQGLAEKKPLNPLSLLEIIISSKLELNKENIIFND

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=339, Percent_Identity=28.9085545722714, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786579, Length=365, Percent_Identity=26.5753424657534, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 90719; Mature: 90719

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIALICGGPSLERGISLNSARSVLDHLASEFIEIIPIYVDYQNFFYLISTAQLYSNTP
CEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEHHHHHCCCC
ADFDFKLKKSAQAMNREEFKKILRNIDLVFPVIHGIFGEDGELQKILEEIDVPFVGTGSQ
CCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCHH
ACAEMFCKYHASQILHQNRFPAFMKLEVCPETNDLAPIKSFFEQHQLKRAIVKPTMGGSS
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IGVTSVQTPEDAFEKIKMLCQQGIAKRALIEPFCEGTEFTVVVLQNQQSKPVALIPTEIE
CCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCEE
MSYQNHQIFDYRKKYLPTNQTTYHTPARFDETIIQGIRQKAEAIFTLFHMRDFARLDGWL
ECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LNDGTIYFTDLNPISGMEQNSFLFRQASLLGLTHRDVFRLIITSACLRWKLAPPTFVDES
EECCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCHH
MLEKKKVHVLFGGKNAERQVSLMSGTNVWLKLLRSSKYHPIPFFYGKNDLIWQLPYAYTL
HHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEECCCCEEEECCCEEEH
DHTVEEILHRCLAREASNKKLPNYFNSICEQLGIPYNFQETPSSMCLQDFYQLSASNQAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
VFIAMHGGEGENGILQKELEKNQLYYNGSCAKTSSICMDKFLTGQVIEQLKHPNIFSLPK
EEEEEECCCCCCCCHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ECVEAANLFGQSTKYFLEKWNKICISLRSNRLIIKPRQDGCSAGIVLLESGADLEKYFAF
HHHHHHHHHCCHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHH
IQNNVSLIPKGSFSQQKDVIEMPSSTCVEYLLEPYIETDRIILDQGELIHEIKEGWVELT
HHCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHEEH
VGVLEHNQIYRSLNPSITLAEGAVLSLEEKFQGGTGVNLTPPPEKILASEDVSQIKFLIE
HHHHHHHHHHHHCCCCEEEECCCEEEEHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHH
KTADALQIQNYARLDIFYHRETKKMVVIEANTLPGLTPSTVFYQQGLAEKKPLNPLSLLE
HHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHH
IIISSKLELNKENIIFND
HHHHCCCCCCCCCCEECC
>Mature Secondary Structure
MFKIALICGGPSLERGISLNSARSVLDHLASEFIEIIPIYVDYQNFFYLISTAQLYSNTP
CEEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEHHHHHCCCC
ADFDFKLKKSAQAMNREEFKKILRNIDLVFPVIHGIFGEDGELQKILEEIDVPFVGTGSQ
CCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCHH
ACAEMFCKYHASQILHQNRFPAFMKLEVCPETNDLAPIKSFFEQHQLKRAIVKPTMGGSS
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IGVTSVQTPEDAFEKIKMLCQQGIAKRALIEPFCEGTEFTVVVLQNQQSKPVALIPTEIE
CCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEECCEE
MSYQNHQIFDYRKKYLPTNQTTYHTPARFDETIIQGIRQKAEAIFTLFHMRDFARLDGWL
ECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
LNDGTIYFTDLNPISGMEQNSFLFRQASLLGLTHRDVFRLIITSACLRWKLAPPTFVDES
EECCEEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCHH
MLEKKKVHVLFGGKNAERQVSLMSGTNVWLKLLRSSKYHPIPFFYGKNDLIWQLPYAYTL
HHCCCCEEEEECCCCCCCEEEEECCCHHHHHHHHCCCCCCCCEEECCCCEEEECCCEEEH
DHTVEEILHRCLAREASNKKLPNYFNSICEQLGIPYNFQETPSSMCLQDFYQLSASNQAF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEE
VFIAMHGGEGENGILQKELEKNQLYYNGSCAKTSSICMDKFLTGQVIEQLKHPNIFSLPK
EEEEEECCCCCCCCHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ECVEAANLFGQSTKYFLEKWNKICISLRSNRLIIKPRQDGCSAGIVLLESGADLEKYFAF
HHHHHHHHHCCHHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHH
IQNNVSLIPKGSFSQQKDVIEMPSSTCVEYLLEPYIETDRIILDQGELIHEIKEGWVELT
HHCCCEEEECCCCCCCCHHHHCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHEEH
VGVLEHNQIYRSLNPSITLAEGAVLSLEEKFQGGTGVNLTPPPEKILASEDVSQIKFLIE
HHHHHHHHHHHHCCCCEEEECCCEEEEHHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHH
KTADALQIQNYARLDIFYHRETKKMVVIEANTLPGLTPSTVFYQQGLAEKKPLNPLSLLE
HHHHHHHHCCCEEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHH
IIISSKLELNKENIIFND
HHHHCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA