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The map label for this gene is yibK [C]

Identifier: 46446016

GI number: 46446016

Start: 508114

End: 508560

Strand: Reverse

Name: yibK [C]

Synonym: pc0382

Alternate gene names: 46446016

Gene position: 508560-508114 (Counterclockwise)

Preceding gene: 46446017

Following gene: 46446014

Centisome position: 21.06

GC content: 37.14

Gene sequence:

>447_bases
ATGAAAATTATTTTATTCCAACCTCAAATTCCCCAAAATGCTGGTAATATTGTAAGAACTTGTGCCGTTACTGGAACAGA
TTTAATGATGGTTCACCCTTTGGGATTCAGTACGCATGATCGATGGCTAAAGCGAGCAGGTCTAGATTATTGGGAAGGTG
TCAGCGTCTCATTTATTGAAAATTTAGAAAATTACCTAGAAAATCAACAAGCTTCTTTTTACTTTTTCTCTAGCAAAGCG
ACAACTCTTTATACTGAAGTTTCTTATGCTGCAGATTCTTTTCTGATATTTGGCTCAGAAACTAGCGGTCTCCCTTCCAT
TTTTCAAGAAAAATGGGGAAATCGATTTGTTAAAATCCCTATGAAGCCAAAAGTACGTTGTTTAAATCTTGCTACTTCTG
CCGGTATCGCCTTGTATGAAGCGTGGCGTCAACAAGGATTTATGTAA

Upstream 100 bases:

>100_bases
ATACTTCTGCTAAAAATTAATAAATTGCATTAACTCCGATCTCATTGAAAATTTGCAAGACTTTAGGCTTGCAAATTTTC
ATTAAATTTCCTTTTCCTCT

Downstream 100 bases:

>100_bases
CAATAATATTAAGCCTTTATTAACTTAGTTTTAAGCCTCAAGTTCTTCTTTTACAATCCGGTCGACCATTTTGATAAACG
CTTGCTTTGTTTCTTCTTCA

Product: hypothetical protein

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MKIILFQPQIPQNAGNIVRTCAVTGTDLMMVHPLGFSTHDRWLKRAGLDYWEGVSVSFIENLENYLENQQASFYFFSSKA
TTLYTEVSYAADSFLIFGSETSGLPSIFQEKWGNRFVKIPMKPKVRCLNLATSAGIALYEAWRQQGFM

Sequences:

>Translated_148_residues
MKIILFQPQIPQNAGNIVRTCAVTGTDLMMVHPLGFSTHDRWLKRAGLDYWEGVSVSFIENLENYLENQQASFYFFSSKA
TTLYTEVSYAADSFLIFGSETSGLPSIFQEKWGNRFVKIPMKPKVRCLNLATSAGIALYEAWRQQGFM
>Mature_148_residues
MKIILFQPQIPQNAGNIVRTCAVTGTDLMMVHPLGFSTHDRWLKRAGLDYWEGVSVSFIENLENYLENQQASFYFFSSKA
TTLYTEVSYAADSFLIFGSETSGLPSIFQEKWGNRFVKIPMKPKVRCLNLATSAGIALYEAWRQQGFM

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=149, Percent_Identity=40.2684563758389, Blast_Score=126, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16821; Mature: 16821

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIILFQPQIPQNAGNIVRTCAVTGTDLMMVHPLGFSTHDRWLKRAGLDYWEGVSVSFIE
CEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHH
NLENYLENQQASFYFFSSKATTLYTEVSYAADSFLIFGSETSGLPSIFQEKWGNRFVKIP
HHHHHHCCCCCEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEC
MKPKVRCLNLATSAGIALYEAWRQQGFM
CCCCEEEEEEHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKIILFQPQIPQNAGNIVRTCAVTGTDLMMVHPLGFSTHDRWLKRAGLDYWEGVSVSFIE
CEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHH
NLENYLENQQASFYFFSSKATTLYTEVSYAADSFLIFGSETSGLPSIFQEKWGNRFVKIP
HHHHHHCCCCCEEEEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEC
MKPKVRCLNLATSAGIALYEAWRQQGFM
CCCCEEEEEEHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]