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The map label for this gene is truA

Identifier: 46445962

GI number: 46445962

Start: 448130

End: 448900

Strand: Direct

Name: truA

Synonym: pc0328

Alternate gene names: 46445962

Gene position: 448130-448900 (Clockwise)

Preceding gene: 46445961

Following gene: 46445964

Centisome position: 18.56

GC content: 35.93

Gene sequence:

>771_bases
ATGCATTGCTATAAATTAACGATAGCTTACGATGGAACGAACTACAGTGGATGGCAAATCCAACCTAATGCTTCTTCCAT
CCAACAAAAAATTCAAGAAGCTCTTTGCATTCTTTTAAAAAAAGAAAAAGTTGTTTTGGTAGGTTCAGGACGCACGGATG
CTGGAGTTCATGCCAAAGGGCAAGTAGCTCATTTTCATTTTCAAGACTATATCGATCTATCACGCCTTCACGTTTCCTTA
AATGGATTATTGCCGCGCGATATTCGTATTAAAGCAGTTGAGCCTGTTTCACCTCGGTTTCATTCGCAGTACAGTGCAAT
TCGAAAAGAATATCATTATTATTTGCATTTAAATAAAGTGATGGACCCTTTTCAACGTTTATATAGCTGGCATTTCCAAA
GAAAAATAGATGTAAATATATTAAAAAAAGCGGCTATTTTATTTACGGGCACTCATGATTTTACCTCATTTGCTAATGAA
GCTCACAGAGGAACTGCAGCTAAAAATCCAGTAAGAACTCTTTATCGTTTAGATATCAAACCAAACGAAGGAGGCCTTCG
ATTAGAATTTGAAGGGGATGGATTTTTGTATAAGATGGTAAGAAACATCGTTGGAACTTTAATGGATGTTGCTTCACATA
AAAGAGCTATAGAAGAAATTAATCAGATTTTTGCTGCTAAAAATCGTCGTCAGGCAAGCCTTGCTGCTCCACCTGAAGGA
CTTTTTCTTATTCAAGTTTTTTACGAAAATGAAAATGGATGTTTGGATTAA

Upstream 100 bases:

>100_bases
TTAATAGATAAACCAGTCAAAGTGGTTGAGGGATCTTATCTTAATATTAAAATTACAACACCAGAAGATCTTCTGATCGC
TCAAAGTATTTTAAAAAATC

Downstream 100 bases:

>100_bases
TTTTAATTTTTTTAAGGATGATTATTTTCTGTAATCGTATTAAAATTTGGGTAATAGCTTAATTCCGGAGCTAATACTTC
ATTTAAGTAAGGGGAATTAG

Product: putative tRNA pseudouridylate synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I 1; tRNA-uridine isomerase I 1

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MHCYKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEKVVLVGSGRTDAGVHAKGQVAHFHFQDYIDLSRLHVSL
NGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANE
AHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAAPPEG
LFLIQVFYENENGCLD

Sequences:

>Translated_256_residues
MHCYKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEKVVLVGSGRTDAGVHAKGQVAHFHFQDYIDLSRLHVSL
NGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANE
AHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAAPPEG
LFLIQVFYENENGCLD
>Mature_256_residues
MHCYKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEKVVLVGSGRTDAGVHAKGQVAHFHFQDYIDLSRLHVSL
NGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKVMDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANE
AHRGTAAKNPVRTLYRLDIKPNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAAPPEG
LFLIQVFYENENGCLD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=29.7101449275362, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI199559785, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=32.6530612244898, Blast_Score=143, Evalue=1e-35,
Organism=Drosophila melanogaster, GI116007752, Length=311, Percent_Identity=26.3665594855305, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA1_PARUW (Q6MEE7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007327.1
- ProteinModelPortal:   Q6MEE7
- SMR:   Q6MEE7
- STRING:   Q6MEE7
- GeneID:   2780741
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0328
- NMPDR:   fig|264201.1.peg.328
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   CLSK2762134
- BioCyc:   CPRO264201:PC0328-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29303; Mature: 29303

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCYKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEKVVLVGSGRTDAGVHAKG
CCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCC
QVAHFHFQDYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV
CEEEEEHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEEHHH
MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK
HHHHHHHHHHHHCCEECHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEC
PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAAPPEG
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LFLIQVFYENENGCLD
EEEEEEEEECCCCCCC
>Mature Secondary Structure
MHCYKLTIAYDGTNYSGWQIQPNASSIQQKIQEALCILLKKEKVVLVGSGRTDAGVHAKG
CCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHEECCCEEEEECCCCCCCCCCCC
QVAHFHFQDYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV
CEEEEEHHHHHHHHHEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEEEEEHHH
MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK
HHHHHHHHHHHHCCEECHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCCCCEEEEEEEEC
PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAAPPEG
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LFLIQVFYENENGCLD
EEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA