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The map label for this gene is ispD

Identifier: 46445961

GI number: 46445961

Start: 447451

End: 448143

Strand: Direct

Name: ispD

Synonym: pc0327

Alternate gene names: 46445961

Gene position: 447451-448143 (Clockwise)

Preceding gene: 46445960

Following gene: 46445962

Centisome position: 18.53

GC content: 35.21

Gene sequence:

>693_bases
ATGGAACATCAACTAATTCCTTTTACAGTTGTTCTTTTAGCTGGTGGAATGGGAACCAGAATGAAAAATCTTATTCCCAA
ACAATATCTAACTATTCAAGAAAAACCTATTGCTTTACATAGCTTTGAGAAATTGGCTTCAATGCCTGAAATTGAGCAAA
TGATTGTTGTTTGCGAACCTCAATACGAACAACTTTTTACAGTCTATAAGTTGAATAAGCCTCTTATTTTTGCGAGACCA
GGATTGCGTAGACAAGATTCTTTATGGAATGGAATTAAATTAATAGCTGGCAATCCCTTGGTTTGTATCCATGATTCTGC
CCGTCCTTTTATTGAAATCGATCGGATTAGACAAACTGTTGCGGAAGCGGAAAAATGGGGGGCTGCTGTATTGGGTGTAC
GAGTAAAAGCGACGATTAAGATTTGTGAAGAAAAGCAATTTATCGTCAATACTCCAAATCGCGCATTTCTTTGGGAAATG
CAAACACCTCAAATTGTGAGGCTAAAACTTCTTTATGACGGTTTTTCTGTTGCTCAAAAAAATGAATTAACTGTAAGTGA
TGATGTTTCCTTAGTGGAATTAATAGATAAACCAGTCAAAGTGGTTGAGGGATCTTATCTTAATATTAAAATTACAACAC
CAGAAGATCTTCTGATCGCTCAAAGTATTTTAAAAAATCATGCATTGCTATAA

Upstream 100 bases:

>100_bases
AAAAAATGGGCTTACATCAATCGTGGAATTGGTAGTGTTATCCATTTTCGTTGGTTTGCTACGCCTGAGTTGACTTTGAT
GACTTTAAAACAAGGATAAG

Downstream 100 bases:

>100_bases
ATTAACGATAGCTTACGATGGAACGAACTACAGTGGATGGCAAATCCAACCTAATGCTTCTTCCATCCAACAAAAAATTC
AAGAAGCTCTTTGCATTCTT

Product: putative 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MEHQLIPFTVVLLAGGMGTRMKNLIPKQYLTIQEKPIALHSFEKLASMPEIEQMIVVCEPQYEQLFTVYKLNKPLIFARP
GLRRQDSLWNGIKLIAGNPLVCIHDSARPFIEIDRIRQTVAEAEKWGAAVLGVRVKATIKICEEKQFIVNTPNRAFLWEM
QTPQIVRLKLLYDGFSVAQKNELTVSDDVSLVELIDKPVKVVEGSYLNIKITTPEDLLIAQSILKNHALL

Sequences:

>Translated_230_residues
MEHQLIPFTVVLLAGGMGTRMKNLIPKQYLTIQEKPIALHSFEKLASMPEIEQMIVVCEPQYEQLFTVYKLNKPLIFARP
GLRRQDSLWNGIKLIAGNPLVCIHDSARPFIEIDRIRQTVAEAEKWGAAVLGVRVKATIKICEEKQFIVNTPNRAFLWEM
QTPQIVRLKLLYDGFSVAQKNELTVSDDVSLVELIDKPVKVVEGSYLNIKITTPEDLLIAQSILKNHALL
>Mature_230_residues
MEHQLIPFTVVLLAGGMGTRMKNLIPKQYLTIQEKPIALHSFEKLASMPEIEQMIVVCEPQYEQLFTVYKLNKPLIFARP
GLRRQDSLWNGIKLIAGNPLVCIHDSARPFIEIDRIRQTVAEAEKWGAAVLGVRVKATIKICEEKQFIVNTPNRAFLWEM
QTPQIVRLKLLYDGFSVAQKNELTVSDDVSLVELIDKPVKVVEGSYLNIKITTPEDLLIAQSILKNHALL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=25.8620689655172, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1789104, Length=218, Percent_Identity=32.5688073394495, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_PARUW (Q6MEE8)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007326.1
- ProteinModelPortal:   Q6MEE8
- STRING:   Q6MEE8
- GeneID:   2780541
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0327
- NMPDR:   fig|264201.1.peg.327
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   NTIPREN
- PhylomeDB:   Q6MEE8
- BioCyc:   CPRO264201:PC0327-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26101; Mature: 26101

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHQLIPFTVVLLAGGMGTRMKNLIPKQYLTIQEKPIALHSFEKLASMPEIEQMIVVCEP
CCCCCHHHEEEECCCCCCHHHHHHCCHHHEEECCCCCHHHHHHHHHCCCCCCEEEEEECC
QYEQLFTVYKLNKPLIFARPGLRRQDSLWNGIKLIAGNPLVCIHDSARPFIEIDRIRQTV
CHHHEEEEEECCCCEEEECCCCCCCCHHHCCEEEEECCCEEEEECCCCCCEEHHHHHHHH
AEAEKWGAAVLGVRVKATIKICEEKQFIVNTPNRAFLWEMQTPQIVRLKLLYDGFSVAQK
HHHHHCCEEEEEEEEEEEEEEECCCEEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCC
NELTVSDDVSLVELIDKPVKVVEGSYLNIKITTPEDLLIAQSILKNHALL
CCCEECCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEHQLIPFTVVLLAGGMGTRMKNLIPKQYLTIQEKPIALHSFEKLASMPEIEQMIVVCEP
CCCCCHHHEEEECCCCCCHHHHHHCCHHHEEECCCCCHHHHHHHHHCCCCCCEEEEEECC
QYEQLFTVYKLNKPLIFARPGLRRQDSLWNGIKLIAGNPLVCIHDSARPFIEIDRIRQTV
CHHHEEEEEECCCCEEEECCCCCCCCHHHCCEEEEECCCEEEEECCCCCCEEHHHHHHHH
AEAEKWGAAVLGVRVKATIKICEEKQFIVNTPNRAFLWEMQTPQIVRLKLLYDGFSVAQK
HHHHHCCEEEEEEEEEEEEEEECCCEEEEECCCCEEEEEECCCCEEEEEEEECCCCCCCC
NELTVSDDVSLVELIDKPVKVVEGSYLNIKITTPEDLLIAQSILKNHALL
CCCEECCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA