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The map label for this gene is lepA

Identifier: 46445956

GI number: 46445956

Start: 440213

End: 442021

Strand: Reverse

Name: lepA

Synonym: pc0322

Alternate gene names: 46445956

Gene position: 442021-440213 (Counterclockwise)

Preceding gene: 46445958

Following gene: 46445955

Centisome position: 18.31

GC content: 39.47

Gene sequence:

>1809_bases
ATGCATTGCCACAATTTAAACAATATCCGCAATTTCTCAATCATTGCTCATATAGATCACGGAAAATCTACGCTTGCTGA
TCGTCTACTAGAGTTAACTAATACAGTTGCTGCCCGCGATATGCAAGAGCAATTGTTAGATGACATGGATCTGGAAAGAG
AAAGAGGCATTACGATCAAAGCCCACCCTGTCACAATGTATTATAAAGGGAATGATGGGCAAACTTATCAAATTAACCTC
ATTGACACTCCAGGCCACGTTGACTTTACTTATGAAGTTTCGCGTTCTTTAGCGGCCTGTGAAGGAGCCATCCTTGTTGT
TGATGCAGGTCAAGGCGTGCAAGCACAGAGCCTAGCCAACGTACATTTAGCTTTGGAAAGAAATCTAGAAATTGTTCCTG
TTATCAACAAAATTGATCTTCCAGCAGCCGATGTAGAAGGAGTGCGTAAACAAATCGAAGATGTGATTGGATTAGATGCC
TCAGATGCAATTGGTTGTTCCGCAAAATCTGGTTTAGGTGTCAAAGATGTTCTTGAGAGAATTCTCACAGCAGTACCTCC
TCCCCAAGAGCCAAAAGACAATTTATTAAGAGCGCTTGTTTTTGACTCTCACTATGATGTTTATCGAGGTGTCATGGTTT
ATATTCGTGTCATGAGTGGAGAAATCAAGCGGGGCTCTCTCATTAAAATGATGGCCACCAACAAAAACTTTGAAGTATTA
GAAGTGGGAATGTTTACCCCTAAAGAAAAACCTGTTGAACAACTCCGACCCGGAGAAGTCGGTTATATGATTGCCAATAT
CAAAACCCCTTCTGATGTCAAAATTGGCGATACCATTACTCTGCAAAAATATCCTGCCCCTGAAGCACTTCCAGGTTTTA
AAATCATTTCTCCCGTAGTCTTTGCAGGAATCTATCCCATTGACGCAACGGAATTTGAAGGGCTCAGAGATGCATTAGGT
AAGCTTCAATTGAATGATTCTGCTTTGCATATTGAGCAAGAAAGTAGTACAGCACTTGGTTTTGGTTTTCGATGTGGATT
CCTTGGGTTGCTACACTTAGAGATAGTTTTTGAGCGAATTCAAAGAGAATTTAACATTGATATTATTTCTACAGCTCCTA
GTGTCATTTATCGTTTTACACTTGATGATGGAGTAGTCAAAACTGTTGACAACCCCGCTCATTACCCAGATCCCACATTT
ATTCGAATGGTCGAAGAACCGTGGGTAAAATGTCACATCATGATTCCATCCGAATATTTAGGAGCTATCATGAACCTAGG
AATGGATAAACGAGGAGTCTGCACGAAAACAGAGACCATGGATGCTCGTAGATTATTATTAACATATCGTCTTCCTCTCA
ATGAAATCATTACAGACTTTAATGACAAGTTAAAATCGATTACGAAAGGTTATGGATCTTTTGATTATGAATTTGATTGC
TATGAACCAAGTGAAATAATCAAACTTGAAATTCGTGTTAATGAAGAACCTGTTGATGCCTTTTCTTGCTTAGTACACAG
AACTAAAGCAGAGAGTAAAGGACGAGCTATCTGTGCTAAATTAGTTGAAGTTATTCCCATGCAGCTCTTTAAGGTTCCTA
TTCAAGCAGCTATTGGCGGAAAAGTTGTGGCGCGAGAAACAATTCGAGCAATCACCAAAAATGTCACAGCAAAATGCTAC
GGGGGAGACATTTCTCGTAAACGTAAATTATGGGAAAAGCAAAAAAAGGGTAAAAAACGGATGAAGGAAATCGGAAAAGT
AAACATTCCTCAAAGTGCCTTTATGGAAGTATTAAAAGCTGGAGATTAA

Upstream 100 bases:

>100_bases
AGATATCTATTGTTCTTTTACCCCATGCAATTCGCTTGAAATGTTTCCTTAAATCCATTAAATTTGATTCTCTCCCAACC
ATTTTGGAAATCAATCATCT

Downstream 100 bases:

>100_bases
TTTTGGAGAGTTGGCAAAAGATAAATCTATTCGATCAAATCCATTTACGTTATTCTTTTGCAAATTTCTAGAGGGTCTAT
GTTGATTGATCACGCTGTTA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MHCHNLNNIRNFSIIAHIDHGKSTLADRLLELTNTVAARDMQEQLLDDMDLERERGITIKAHPVTMYYKGNDGQTYQINL
IDTPGHVDFTYEVSRSLAACEGAILVVDAGQGVQAQSLANVHLALERNLEIVPVINKIDLPAADVEGVRKQIEDVIGLDA
SDAIGCSAKSGLGVKDVLERILTAVPPPQEPKDNLLRALVFDSHYDVYRGVMVYIRVMSGEIKRGSLIKMMATNKNFEVL
EVGMFTPKEKPVEQLRPGEVGYMIANIKTPSDVKIGDTITLQKYPAPEALPGFKIISPVVFAGIYPIDATEFEGLRDALG
KLQLNDSALHIEQESSTALGFGFRCGFLGLLHLEIVFERIQREFNIDIISTAPSVIYRFTLDDGVVKTVDNPAHYPDPTF
IRMVEEPWVKCHIMIPSEYLGAIMNLGMDKRGVCTKTETMDARRLLLTYRLPLNEIITDFNDKLKSITKGYGSFDYEFDC
YEPSEIIKLEIRVNEEPVDAFSCLVHRTKAESKGRAICAKLVEVIPMQLFKVPIQAAIGGKVVARETIRAITKNVTAKCY
GGDISRKRKLWEKQKKGKKRMKEIGKVNIPQSAFMEVLKAGD

Sequences:

>Translated_602_residues
MHCHNLNNIRNFSIIAHIDHGKSTLADRLLELTNTVAARDMQEQLLDDMDLERERGITIKAHPVTMYYKGNDGQTYQINL
IDTPGHVDFTYEVSRSLAACEGAILVVDAGQGVQAQSLANVHLALERNLEIVPVINKIDLPAADVEGVRKQIEDVIGLDA
SDAIGCSAKSGLGVKDVLERILTAVPPPQEPKDNLLRALVFDSHYDVYRGVMVYIRVMSGEIKRGSLIKMMATNKNFEVL
EVGMFTPKEKPVEQLRPGEVGYMIANIKTPSDVKIGDTITLQKYPAPEALPGFKIISPVVFAGIYPIDATEFEGLRDALG
KLQLNDSALHIEQESSTALGFGFRCGFLGLLHLEIVFERIQREFNIDIISTAPSVIYRFTLDDGVVKTVDNPAHYPDPTF
IRMVEEPWVKCHIMIPSEYLGAIMNLGMDKRGVCTKTETMDARRLLLTYRLPLNEIITDFNDKLKSITKGYGSFDYEFDC
YEPSEIIKLEIRVNEEPVDAFSCLVHRTKAESKGRAICAKLVEVIPMQLFKVPIQAAIGGKVVARETIRAITKNVTAKCY
GGDISRKRKLWEKQKKGKKRMKEIGKVNIPQSAFMEVLKAGD
>Mature_602_residues
MHCHNLNNIRNFSIIAHIDHGKSTLADRLLELTNTVAARDMQEQLLDDMDLERERGITIKAHPVTMYYKGNDGQTYQINL
IDTPGHVDFTYEVSRSLAACEGAILVVDAGQGVQAQSLANVHLALERNLEIVPVINKIDLPAADVEGVRKQIEDVIGLDA
SDAIGCSAKSGLGVKDVLERILTAVPPPQEPKDNLLRALVFDSHYDVYRGVMVYIRVMSGEIKRGSLIKMMATNKNFEVL
EVGMFTPKEKPVEQLRPGEVGYMIANIKTPSDVKIGDTITLQKYPAPEALPGFKIISPVVFAGIYPIDATEFEGLRDALG
KLQLNDSALHIEQESSTALGFGFRCGFLGLLHLEIVFERIQREFNIDIISTAPSVIYRFTLDDGVVKTVDNPAHYPDPTF
IRMVEEPWVKCHIMIPSEYLGAIMNLGMDKRGVCTKTETMDARRLLLTYRLPLNEIITDFNDKLKSITKGYGSFDYEFDC
YEPSEIIKLEIRVNEEPVDAFSCLVHRTKAESKGRAICAKLVEVIPMQLFKVPIQAAIGGKVVARETIRAITKNVTAKCY
GGDISRKRKLWEKQKKGKKRMKEIGKVNIPQSAFMEVLKAGD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=49.0033222591362, Blast_Score=598, Evalue=1e-171,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=44.1176470588235, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=42.7631578947368, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.7631578947368, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.2777777777778, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.7631578947368, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI18390331, Length=155, Percent_Identity=40.6451612903226, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI217272894, Length=164, Percent_Identity=34.1463414634146, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI217272892, Length=164, Percent_Identity=34.1463414634146, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=42.1052631578947, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=42.1052631578947, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=42.1052631578947, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI94966752, Length=135, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI223555963, Length=296, Percent_Identity=26.0135135135135, Blast_Score=67, Evalue=7e-11,
Organism=Homo sapiens, GI5729864, Length=296, Percent_Identity=26.0135135135135, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=52.0134228187919, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=28.3495145631068, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=38.0645161290323, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790835, Length=165, Percent_Identity=29.0909090909091, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789559, Length=253, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI2367247, Length=283, Percent_Identity=26.1484098939929, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=39.7058823529412, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=491, Percent_Identity=26.6802443991853, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17533571, Length=158, Percent_Identity=38.6075949367089, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=153, Percent_Identity=33.9869281045752, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.1794871794872, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.8208955223881, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.8208955223881, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566303, Length=308, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=294, Percent_Identity=28.2312925170068, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=312, Percent_Identity=25, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=42.358803986711, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=38.7978142076503, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=41.0958904109589, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=41.0958904109589, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=40, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=36.1702127659575, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=45.575959933222, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=39.2638036809816, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.1621621621622, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.1621621621622, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.1621621621622, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21357743, Length=161, Percent_Identity=35.4037267080745, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.7350993377483, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28572034, Length=257, Percent_Identity=28.7937743190661, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921738, Length=292, Percent_Identity=29.4520547945205, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_PARUW (Q6MEF3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007321.1
- ProteinModelPortal:   Q6MEF3
- SMR:   Q6MEF3
- STRING:   Q6MEF3
- GeneID:   2780784
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0322
- NMPDR:   fig|264201.1.peg.322
- eggNOG:   COG0481
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   CPRO264201:PC0322-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67181; Mature: 67181

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCHNLNNIRNFSIIAHIDHGKSTLADRLLELTNTVAARDMQEQLLDDMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEE
AHPVTMYYKGNDGQTYQINLIDTPGHVDFTYEVSRSLAACEGAILVVDAGQGVQAQSLAN
EEEEEEEEECCCCCEEEEEEEECCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VHLALERNLEIVPVINKIDLPAADVEGVRKQIEDVIGLDASDAIGCSAKSGLGVKDVLER
EEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
ILTAVPPPQEPKDNLLRALVFDSHYDVYRGVMVYIRVMSGEIKRGSLIKMMATNKNFEVL
HHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEE
EVGMFTPKEKPVEQLRPGEVGYMIANIKTPSDVKIGDTITLQKYPAPEALPGFKIISPVV
EEECCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
FAGIYPIDATEFEGLRDALGKLQLNDSALHIEQESSTALGFGFRCGFLGLLHLEIVFERI
HHCCCCCCCHHHHHHHHHHCCEEECCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHH
QREFNIDIISTAPSVIYRFTLDDGVVKTVDNPAHYPDPTFIRMVEEPWVKCHIMIPSEYL
HHHCCEEEEECCCCEEEEEEECCCCEEECCCCCCCCCCHHEEEECCCCEEEEEEECHHHH
GAIMNLGMDKRGVCTKTETMDARRLLLTYRLPLNEIITDFNDKLKSITKGYGSFDYEFDC
HHHHHCCCCCCCCCCCCHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEECC
YEPSEIIKLEIRVNEEPVDAFSCLVHRTKAESKGRAICAKLVEVIPMQLFKVPIQAAIGG
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVVARETIRAITKNVTAKCYGGDISRKRKLWEKQKKGKKRMKEIGKVNIPQSAFMEVLKA
CHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GD
CC
>Mature Secondary Structure
MHCHNLNNIRNFSIIAHIDHGKSTLADRLLELTNTVAARDMQEQLLDDMDLERERGITIK
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEE
AHPVTMYYKGNDGQTYQINLIDTPGHVDFTYEVSRSLAACEGAILVVDAGQGVQAQSLAN
EEEEEEEEECCCCCEEEEEEEECCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VHLALERNLEIVPVINKIDLPAADVEGVRKQIEDVIGLDASDAIGCSAKSGLGVKDVLER
EEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
ILTAVPPPQEPKDNLLRALVFDSHYDVYRGVMVYIRVMSGEIKRGSLIKMMATNKNFEVL
HHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEE
EVGMFTPKEKPVEQLRPGEVGYMIANIKTPSDVKIGDTITLQKYPAPEALPGFKIISPVV
EEECCCCCCCCHHHCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
FAGIYPIDATEFEGLRDALGKLQLNDSALHIEQESSTALGFGFRCGFLGLLHLEIVFERI
HHCCCCCCCHHHHHHHHHHCCEEECCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHH
QREFNIDIISTAPSVIYRFTLDDGVVKTVDNPAHYPDPTFIRMVEEPWVKCHIMIPSEYL
HHHCCEEEEECCCCEEEEEEECCCCEEECCCCCCCCCCHHEEEECCCCEEEEEEECHHHH
GAIMNLGMDKRGVCTKTETMDARRLLLTYRLPLNEIITDFNDKLKSITKGYGSFDYEFDC
HHHHHCCCCCCCCCCCCHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEECC
YEPSEIIKLEIRVNEEPVDAFSCLVHRTKAESKGRAICAKLVEVIPMQLFKVPIQAAIGG
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVVARETIRAITKNVTAKCYGGDISRKRKLWEKQKKGKKRMKEIGKVNIPQSAFMEVLKA
CHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA