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The map label for this gene is dnaA [H]

Identifier: 46445941

GI number: 46445941

Start: 424280

End: 425665

Strand: Reverse

Name: dnaA [H]

Synonym: pc0307

Alternate gene names: 46445941

Gene position: 425665-424280 (Counterclockwise)

Preceding gene: 46445943

Following gene: 46445940

Centisome position: 17.63

GC content: 35.43

Gene sequence:

>1386_bases
GTGGAAACGTTGTTCATAACTTTTTTAAGGAACAAAAATATGCTAACTAGCGAAACACAAAATGTCTGGACACAGTTTTT
ACAATTTGTAAAAACACGCTGTTCACCAGCCGCTTTTGGAAACTGGTTAGCTCCTATTCGAGTGATTGATTGTTCGACGG
AAGAAGTCACTCTAGAAATTCCAAATATTTTTGTACAAGAATATTTGCTCTCGAATTATAAAAAAGATTTATGTGCGTTT
TTGCCAGTCGATATGTCAGGTGAACCTGCCATTCGATTTATTATTGCTCCACCGCAAAAAAAAATCATTCCACCCAACCA
TTTTTCTATTTCTTCTTCTCAAAAAGAAGAGCAGTCGCCCAATTCTGATGTTAAACTCAATAATAATTACCGTTTTGAAA
ATTTTATTGAAGGTCCAACTAATCAATTTGTCAAATCTGCTGCAATGGGAGTTGCTTTACGTCCAGGCCAATCATATAAT
CCTCTTTTTATTCACGGAGGAGTGGGATTAGGTAAAACACATATTTTACATAGTATTGGACATTATATTAAAGAAAATCA
CAAGAAGTTGCGCGTACAATGCATTACGACAGAAGCTTTTATCAATGATTTAGTAGATAGCTTAAGAAATAAATCTGTCG
ATCGAATGAAAAAATTCTATCGATCAGAAATTGATGTATTGCTTGTGGACGATATTCAATTTTTACAAAACCGATTAAAT
TTTGAAGAAGAGTTATCCTATACTTTTGAAGCTTTAAAAAATAAAGGAGCTCAAATTGTTATCACAAGTGATAAACCTCC
TTCTCTTTTAAAGCTTTCGGAACGAACAATTGGAAAAATGGAAGGGGGTCTTGTAGCTCATATGGGAATTCCCGAACTCG
AAACTCGGGTAGCCATTTTACAGTATAAGGCTGAGCAAAAAGGTCTACATATTCCTCATAAAGTAGCTTTTTTTATTGCT
GAACATATTCATAATAACGTTCGGCAATTAGAAGGAGCAATTAACCGATTAAGCGCACATTGTCGTCTTCTCGACTTAAA
TATTACAGAAGAACTTGTTTCGCGAACTCTACGTGAAATGTTGCAACAAGCTCCCAGAGAAAAAATCTCTGTTGAACAAA
TTCTTAAAAGTGTCGCTGCTGTATTTCAAGTTCGCGTCAGTGATTTAAGAGGATCAACTCGGACAAAAGATATCGCTCTT
CCTCGTCAAGTCGCTATGTATTTAGCTAAAGAAATGATCAACGAATCTTTAATCATGTTAGGAGCGTCTTTTGGCAAAAC
TCACTCAACAATTCTGCATGCTTGCAAAAACATTGAGAAAAAAGTCGCTAGTGATGAAACCTTACGACGCCAAATTGGCA
TGGTACGTCGAAATATTGAATCTTAA

Upstream 100 bases:

>100_bases
ACGGTTTACTTATAAAATGGAATAGACCATCAAGAAAATTACTTGCCAGCTAAGATTAGCTCATGGTAAATTCTTTCATG
AAATCACCCCAGAATGTGCT

Downstream 100 bases:

>100_bases
TTATTAGTTAATTTTAATGATTTCTTACAATATTCATACACTTATATAAACGAACTAAAGATAGACATTAAATGCGTTAT
CTCTAAAATAAAAGCTACAT

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
METLFITFLRNKNMLTSETQNVWTQFLQFVKTRCSPAAFGNWLAPIRVIDCSTEEVTLEIPNIFVQEYLLSNYKKDLCAF
LPVDMSGEPAIRFIIAPPQKKIIPPNHFSISSSQKEEQSPNSDVKLNNNYRFENFIEGPTNQFVKSAAMGVALRPGQSYN
PLFIHGGVGLGKTHILHSIGHYIKENHKKLRVQCITTEAFINDLVDSLRNKSVDRMKKFYRSEIDVLLVDDIQFLQNRLN
FEEELSYTFEALKNKGAQIVITSDKPPSLLKLSERTIGKMEGGLVAHMGIPELETRVAILQYKAEQKGLHIPHKVAFFIA
EHIHNNVRQLEGAINRLSAHCRLLDLNITEELVSRTLREMLQQAPREKISVEQILKSVAAVFQVRVSDLRGSTRTKDIAL
PRQVAMYLAKEMINESLIMLGASFGKTHSTILHACKNIEKKVASDETLRRQIGMVRRNIES

Sequences:

>Translated_461_residues
METLFITFLRNKNMLTSETQNVWTQFLQFVKTRCSPAAFGNWLAPIRVIDCSTEEVTLEIPNIFVQEYLLSNYKKDLCAF
LPVDMSGEPAIRFIIAPPQKKIIPPNHFSISSSQKEEQSPNSDVKLNNNYRFENFIEGPTNQFVKSAAMGVALRPGQSYN
PLFIHGGVGLGKTHILHSIGHYIKENHKKLRVQCITTEAFINDLVDSLRNKSVDRMKKFYRSEIDVLLVDDIQFLQNRLN
FEEELSYTFEALKNKGAQIVITSDKPPSLLKLSERTIGKMEGGLVAHMGIPELETRVAILQYKAEQKGLHIPHKVAFFIA
EHIHNNVRQLEGAINRLSAHCRLLDLNITEELVSRTLREMLQQAPREKISVEQILKSVAAVFQVRVSDLRGSTRTKDIAL
PRQVAMYLAKEMINESLIMLGASFGKTHSTILHACKNIEKKVASDETLRRQIGMVRRNIES
>Mature_461_residues
METLFITFLRNKNMLTSETQNVWTQFLQFVKTRCSPAAFGNWLAPIRVIDCSTEEVTLEIPNIFVQEYLLSNYKKDLCAF
LPVDMSGEPAIRFIIAPPQKKIIPPNHFSISSSQKEEQSPNSDVKLNNNYRFENFIEGPTNQFVKSAAMGVALRPGQSYN
PLFIHGGVGLGKTHILHSIGHYIKENHKKLRVQCITTEAFINDLVDSLRNKSVDRMKKFYRSEIDVLLVDDIQFLQNRLN
FEEELSYTFEALKNKGAQIVITSDKPPSLLKLSERTIGKMEGGLVAHMGIPELETRVAILQYKAEQKGLHIPHKVAFFIA
EHIHNNVRQLEGAINRLSAHCRLLDLNITEELVSRTLREMLQQAPREKISVEQILKSVAAVFQVRVSDLRGSTRTKDIAL
PRQVAMYLAKEMINESLIMLGASFGKTHSTILHACKNIEKKVASDETLRRQIGMVRRNIES

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=447, Percent_Identity=35.5704697986577, Blast_Score=285, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 52426; Mature: 52426

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLFITFLRNKNMLTSETQNVWTQFLQFVKTRCSPAAFGNWLAPIRVIDCSTEEVTLEI
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCEEEEEC
PNIFVQEYLLSNYKKDLCAFLPVDMSGEPAIRFIIAPPQKKIIPPNHFSISSSQKEEQSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
NSDVKLNNNYRFENFIEGPTNQFVKSAAMGVALRPGQSYNPLFIHGGVGLGKTHILHSIG
CCCEEECCCCCCHHHCCCCHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCHHHHHHHHH
HYIKENHKKLRVQCITTEAFINDLVDSLRNKSVDRMKKFYRSEIDVLLVDDIQFLQNRLN
HHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCC
FEEELSYTFEALKNKGAQIVITSDKPPSLLKLSERTIGKMEGGLVAHMGIPELETRVAIL
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHH
QYKAEQKGLHIPHKVAFFIAEHIHNNVRQLEGAINRLSAHCRLLDLNITEELVSRTLREM
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHH
LQQAPREKISVEQILKSVAAVFQVRVSDLRGSTRTKDIALPRQVAMYLAKEMINESLIML
HHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GASFGKTHSTILHACKNIEKKVASDETLRRQIGMVRRNIES
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLFITFLRNKNMLTSETQNVWTQFLQFVKTRCSPAAFGNWLAPIRVIDCSTEEVTLEI
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCEEEEEC
PNIFVQEYLLSNYKKDLCAFLPVDMSGEPAIRFIIAPPQKKIIPPNHFSISSSQKEEQSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCC
NSDVKLNNNYRFENFIEGPTNQFVKSAAMGVALRPGQSYNPLFIHGGVGLGKTHILHSIG
CCCEEECCCCCCHHHCCCCHHHHHHHHHCCEEECCCCCCCCEEEECCCCCCHHHHHHHHH
HYIKENHKKLRVQCITTEAFINDLVDSLRNKSVDRMKKFYRSEIDVLLVDDIQFLQNRLN
HHHHCCCCEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEHHHHHHHHHCC
FEEELSYTFEALKNKGAQIVITSDKPPSLLKLSERTIGKMEGGLVAHMGIPELETRVAIL
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHH
QYKAEQKGLHIPHKVAFFIAEHIHNNVRQLEGAINRLSAHCRLLDLNITEELVSRTLREM
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHH
LQQAPREKISVEQILKSVAAVFQVRVSDLRGSTRTKDIALPRQVAMYLAKEMINESLIML
HHHCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GASFGKTHSTILHACKNIEKKVASDETLRRQIGMVRRNIES
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA