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The map label for this gene is nqrE [H]

Identifier: 46445932

GI number: 46445932

Start: 411269

End: 412012

Strand: Reverse

Name: nqrE [H]

Synonym: pc0298

Alternate gene names: 46445932

Gene position: 412012-411269 (Counterclockwise)

Preceding gene: 46445933

Following gene: 46445931

Centisome position: 17.06

GC content: 38.98

Gene sequence:

>744_bases
ATGTGGATGGGACCCTACAGCGTGCTAAACCTTTTTGGTTTGTTATTACAAGCTGTATTTATTGAGAACTTTTTACTTGT
CAATTTCTTAGGAATGTGTACCTATTTAGCCTGTTCTAATAAGCTAAAAACAGCTAATGGATTGGGTATCGCTGTTGTTT
TTGTATTGACAGTTTCTGGAGTTTTAAATTGGTTTGTGCATCGTTTTGTGACAGGCCCGGGAGCTTTAACCTGGCTAAGT
GCATTCGGAATAGATAGTTCTCAAGTCAATCTCGGTTTTTTGGAGTTTCTACTATTTATCTCTGTGATTGCAGGTTTTAC
ACAAATCCTCGAAATCATCATTGAAAAAGTCTCTCCCGCCCTTTATATGTCGCTTGGACTCTATCTTCCTCTCATTGCAG
TCAATTGTGCGATTTTAGGAGCTTGCTTATTTGCAGTGACTCGAGATTATCCTTTTTTCCCTAACCTTATTTACGTCTTT
GGTTCAGCTGTAGGCTGGTGGCTTGCCATTGCTTTGATTGCAGCTATTCGAGAAAAAATAGCGATATCTAATGTGGTTCC
CGAATTAAAAGGAATGGGGATTACTTTTATCATGTCAGGATTAATGTCTATGGCATTTATGGGGTTTACTGGTATCAAGC
TTGCTGATCCTTCGCCACCAGAAGCTACTTCTGAACAACCTTTAAAAGAATTGGCGTTATCGGATGATTCTTCCTCTCAG
GAAACATTGACGTTAAGCAAATAA

Upstream 100 bases:

>100_bases
TCCTCCGGCAGCTTTTTTTCTGATCGGTGGTCTCATTTACGTATCTAAAAAAATGCAAGGTAATGCTTAATACCATTTAT
TGAAAAGAGGAGACTTTGTC

Downstream 100 bases:

>100_bases
GAATTATCTTAAAAACAGTCCAAAGGATTATTCGTCATTTTTTACAAAAAATTGCCTAAATATTCTTTCAAATTATGCCG
AAGGCAAAAGTTTTCATCAT

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: Na(+)-NQR subunit E; Na(+)-translocating NQR subunit E; NQR complex subunit E; NQR-1 subunit E [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MWMGPYSVLNLFGLLLQAVFIENFLLVNFLGMCTYLACSNKLKTANGLGIAVVFVLTVSGVLNWFVHRFVTGPGALTWLS
AFGIDSSQVNLGFLEFLLFISVIAGFTQILEIIIEKVSPALYMSLGLYLPLIAVNCAILGACLFAVTRDYPFFPNLIYVF
GSAVGWWLAIALIAAIREKIAISNVVPELKGMGITFIMSGLMSMAFMGFTGIKLADPSPPEATSEQPLKELALSDDSSSQ
ETLTLSK

Sequences:

>Translated_247_residues
MWMGPYSVLNLFGLLLQAVFIENFLLVNFLGMCTYLACSNKLKTANGLGIAVVFVLTVSGVLNWFVHRFVTGPGALTWLS
AFGIDSSQVNLGFLEFLLFISVIAGFTQILEIIIEKVSPALYMSLGLYLPLIAVNCAILGACLFAVTRDYPFFPNLIYVF
GSAVGWWLAIALIAAIREKIAISNVVPELKGMGITFIMSGLMSMAFMGFTGIKLADPSPPEATSEQPLKELALSDDSSSQ
ETLTLSK
>Mature_247_residues
MWMGPYSVLNLFGLLLQAVFIENFLLVNFLGMCTYLACSNKLKTANGLGIAVVFVLTVSGVLNWFVHRFVTGPGALTWLS
AFGIDSSQVNLGFLEFLLFISVIAGFTQILEIIIEKVSPALYMSLGLYLPLIAVNCAILGACLFAVTRDYPFFPNLIYVF
GSAVGWWLAIALIAAIREKIAISNVVPELKGMGITFIMSGLMSMAFMGFTGIKLADPSPPEATSEQPLKELALSDDSSSQ
ETLTLSK

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG2209

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=199, Percent_Identity=39.6984924623116, Blast_Score=146, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010967 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 26772; Mature: 26772

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEECCC
>Mature Secondary Structure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CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]