Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 46445901

GI number: 46445901

Start: 380039

End: 380377

Strand: Reverse

Name: ptsN [H]

Synonym: pc0267

Alternate gene names: 46445901

Gene position: 380377-380039 (Counterclockwise)

Preceding gene: 46445902

Following gene: 46445900

Centisome position: 15.75

GC content: 32.74

Gene sequence:

>339_bases
TTGGATAATGAAGAATTATTCTACCAAGCAATTATTGAACGAGAAAAAATTGTTTCAACTGGTATTGGAATGGGGGCTGC
TATTCCACATGCTAAACTCCCTTCCTATGATCGGTTTTTTATTGTGATTGGAATTCTTCAAAAAGCTGTCGAATGGCATG
CTTTAGATGGCGGACCTGTTCGTCTTATTTTTCTGATTGGAGGACCCGATGATAAACAAACCGAATATTTACAATTATTA
TCTAACTTAACTCAAGCTATTAAAAATGAAGAGAGAAGAAAGAAATTATTGACTCTTTCAAATCCAAAAGAAATTATTGA
ATTATTTCAAGAGACTTAA

Upstream 100 bases:

>100_bases
TTCTAACTATCTCGATTCTAATTTGGTCGTTTTTTTAAACACTCCTACACGCAATGAAGCTTTAAGAGAGCTTGTTCATG
TCGCTTATGAATCAGGCAAG

Downstream 100 bases:

>100_bases
AGCTTGCTAAATAAAACCATTCATTGAATAATTAGAAAAGAAATAATTTTAATTTTGTTGTAGATTTTTTTGTGCTATTC
GTTGAACTAAAAATGAAAAT

Product: putative nitrogen regulatory IIA protein (enzyme IIA-ntr) (phosphotransferase enzyme II, A component)

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MDNEELFYQAIIEREKIVSTGIGMGAAIPHAKLPSYDRFFIVIGILQKAVEWHALDGGPVRLIFLIGGPDDKQTEYLQLL
SNLTQAIKNEERRKKLLTLSNPKEIIELFQET

Sequences:

>Translated_112_residues
MDNEELFYQAIIEREKIVSTGIGMGAAIPHAKLPSYDRFFIVIGILQKAVEWHALDGGPVRLIFLIGGPDDKQTEYLQLL
SNLTQAIKNEERRKKLLTLSNPKEIIELFQET
>Mature_112_residues
MDNEELFYQAIIEREKIVSTGIGMGAAIPHAKLPSYDRFFIVIGILQKAVEWHALDGGPVRLIFLIGGPDDKQTEYLQLL
SNLTQAIKNEERRKKLLTLSNPKEIIELFQET

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 12739; Mature: 12739

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNEELFYQAIIEREKIVSTGIGMGAAIPHAKLPSYDRFFIVIGILQKAVEWHALDGGPV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
RLIFLIGGPDDKQTEYLQLLSNLTQAIKNEERRKKLLTLSNPKEIIELFQET
EEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MDNEELFYQAIIEREKIVSTGIGMGAAIPHAKLPSYDRFFIVIGILQKAVEWHALDGGPV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCE
RLIFLIGGPDDKQTEYLQLLSNLTQAIKNEERRKKLLTLSNPKEIIELFQET
EEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]