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The map label for this gene is ptsN [H]

Identifier: 46445900

GI number: 46445900

Start: 379001

End: 379720

Strand: Reverse

Name: ptsN [H]

Synonym: pc0266

Alternate gene names: 46445900

Gene position: 379720-379001 (Counterclockwise)

Preceding gene: 46445901

Following gene: 46445898

Centisome position: 15.73

GC content: 32.5

Gene sequence:

>720_bases
ATGACCCAGGATTTAAAAATTAGGGATGTTGCCGATTTATTGAATGTATCAGAAACAACAATTCGTCGTTGGGTTTCAGA
TAGAAAAATCCCTTCTTATAGTATCAATAAACATCATTATTTTTCTCGCACTGAAATAGAAAATTGGGTGATTTCTCATA
AATTCGATAAAAACCAGAGTAGTTCTCCATTTACACAATCAATCAAAACAACTGTTGCAGAAAAAAAACCTGCTACAGGT
GGAAGTAAACAATTTAGTTTGTTTAGAGCGATTCATAAAGGTGATGTTCTATACAATCTATCTGGTAAAACAAAAGAAGA
AATTATTCGCAAGACAATGAAAAAAACGGCTAAAAATTTAAACGTCGACGCTGAAGTCATGACTGAATTATTATTAGACC
GTGAAAATATGATGCCTACCGCTCTTAATAATGGCGTTGCAATTCCTCATACAAGGGATTCTTTACACGCCCAACATGAT
GCCATTGTTGTTGTATTTTTAGATAAACCTCTTGAATATGGCGCCTTAGATGGTAAACCTGTCAATACATTATTTTTCTT
ATTTGCTGCAGATGATAAACGCCACTTACATTTATTAGCTAAAATTGCTCATTTAAGCAGCCAACCAAAAGCTTTAGAAT
TTTTTAAAACAAAACCCTCTAAAGATAAACTACTAGCTTTTATTAAAGATTGGGAAAGTCAAATTTCACAAATCGTTTAA

Upstream 100 bases:

>100_bases
TTGTCTTATATAAGTGTATAATCCGATAGATATAGTAAGAGTCTGTTTATTCCTATAAACATTCTCAAAATATTATTTAA
TAAATATTAAGTGAGGAAGT

Downstream 100 bases:

>100_bases
TTTTTAATTTCATCAAACGCTATCTTTCTTTTTAAAGGTTCCTTTCAACTAATTGAAAAGGAACCTTTTTTATTTAATAA
TAATTAATATTCAATTAATG

Product: putative nitrogen regulatory IIA protein (enzyme IIA-ntr) (phosphotransferase enzyme II, A component)

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTQDLKIRDVADLLNVSETTIRRWVSDRKIPSYSINKHHYFSRTEIENWVISHKFDKNQSSSPFTQSIKTTVAEKKPATG
GSKQFSLFRAIHKGDVLYNLSGKTKEEIIRKTMKKTAKNLNVDAEVMTELLLDRENMMPTALNNGVAIPHTRDSLHAQHD
AIVVVFLDKPLEYGALDGKPVNTLFFLFAADDKRHLHLLAKIAHLSSQPKALEFFKTKPSKDKLLAFIKDWESQISQIV

Sequences:

>Translated_239_residues
MTQDLKIRDVADLLNVSETTIRRWVSDRKIPSYSINKHHYFSRTEIENWVISHKFDKNQSSSPFTQSIKTTVAEKKPATG
GSKQFSLFRAIHKGDVLYNLSGKTKEEIIRKTMKKTAKNLNVDAEVMTELLLDRENMMPTALNNGVAIPHTRDSLHAQHD
AIVVVFLDKPLEYGALDGKPVNTLFFLFAADDKRHLHLLAKIAHLSSQPKALEFFKTKPSKDKLLAFIKDWESQISQIV
>Mature_238_residues
TQDLKIRDVADLLNVSETTIRRWVSDRKIPSYSINKHHYFSRTEIENWVISHKFDKNQSSSPFTQSIKTTVAEKKPATGG
SKQFSLFRAIHKGDVLYNLSGKTKEEIIRKTMKKTAKNLNVDAEVMTELLLDRENMMPTALNNGVAIPHTRDSLHAQHDA
IVVVFLDKPLEYGALDGKPVNTLFFLFAADDKRHLHLLAKIAHLSSQPKALEFFKTKPSKDKLLAFIKDWESQISQIV

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=122, Percent_Identity=34.4262295081967, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 27257; Mature: 27125

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQDLKIRDVADLLNVSETTIRRWVSDRKIPSYSINKHHYFSRTEIENWVISHKFDKNQS
CCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
SSPFTQSIKTTVAEKKPATGGSKQFSLFRAIHKGDVLYNLSGKTKEEIIRKTMKKTAKNL
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCC
NVDAEVMTELLLDRENMMPTALNNGVAIPHTRDSLHAQHDAIVVVFLDKPLEYGALDGKP
CCCHHHHHHHHHHHCCCCCHHCCCCEECCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCC
VNTLFFLFAADDKRHLHLLAKIAHLSSQPKALEFFKTKPSKDKLLAFIKDWESQISQIV
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQDLKIRDVADLLNVSETTIRRWVSDRKIPSYSINKHHYFSRTEIENWVISHKFDKNQS
CCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
SSPFTQSIKTTVAEKKPATGGSKQFSLFRAIHKGDVLYNLSGKTKEEIIRKTMKKTAKNL
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCC
NVDAEVMTELLLDRENMMPTALNNGVAIPHTRDSLHAQHDAIVVVFLDKPLEYGALDGKP
CCCHHHHHHHHHHHCCCCCHHCCCCEECCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCC
VNTLFFLFAADDKRHLHLLAKIAHLSSQPKALEFFKTKPSKDKLLAFIKDWESQISQIV
CCEEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]