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The map label for this gene is ygcA [C]

Identifier: 46445882

GI number: 46445882

Start: 347735

End: 349039

Strand: Reverse

Name: ygcA [C]

Synonym: pc0248

Alternate gene names: 46445882

Gene position: 349039-347735 (Counterclockwise)

Preceding gene: 46445883

Following gene: 46445880

Centisome position: 14.46

GC content: 32.87

Gene sequence:

>1305_bases
ATGACCCATCTATTGACTATTCATACTCAAGTTGAGGGAGAAATTACAGCGCTTGCTTTTGGAGGTGCAGGAATTTTAAG
ATATCATGGATTTGTAATTTTTGTTCCTTTTACTGCTCCTGGCGATCAAATTATTTGTCGTATCATCGAAATAAAAAAAT
CTTTTGCAGTAGCAGAACTTGTCAAACTCAAACAACCTAGCTCTTTAAGAGTTCAGCCCCCTTGTCCATATTTTGGGAAG
TGTGGAGGTTGCCAACTTCAGCATTTAAACGAACAAACACAATTAAACTATAAACTTACTGCCATCACTGACACTCTTAA
ACGAATTGGTCATCTTAAAGTACCTTCTGTTGAAATGAATACTGCTCAATTGAATTGGGCTTACAGGCGACATATTACCC
TTCATTTAAAATCCTTTAATCAAAGTTTTCAAGCAGGTTATATAGCAACAGATCATTTTTCTCTTATTTCAATCGATACA
TGTCCTATTTTTAATAACCTTCATGACCCTATCATTAGCATATTACAAAAATTTATTAGTACTTTGCCTAATCCCAATCA
GCAAGATGGCCGATTGACATTGTTAAAAAACCAAAATTCTCAATATATTTTATCATTTCAATTTTGTTCATTGAATGAAC
TTAATCGAATTCTGTTTCAAGAGGCTTTAGAAAAATATCCTATTTTTTCGGGTATTCTAGTAACTTCTCCCAAGAAACAG
TGGATAATAGGTAACCCCTACTCTGAACAAAAAATTGAAAATTTAATTTTTCGGTTTACTCCTCAAGCCTTCATACAAAA
TCATCCTGAACAAAGCCTTTATATTTATAAAAAAATTTGTTCGTTAACGATACAACTAAAATCAAAAAAAATTTTAGATC
TTTACTGTGGGTTTGGAATTACCTCTTTACTCCTTGCTAGTCAAGGACATTTCGTAACTGGCATTGAATATAATTCTGAC
GCCATTCGATTTGCGAAAGAAAATAGCAAGTTGAACCATTTGCCTCATGTCGAATTTCTCGAAGGAGATGTAGAAAAAAT
TTTACCTATTTGTTTAAAGTCTCAAAAATGTGTAGATTTAGTTATTGTCAATCCTCCAAGAATTGGCTTATCTAAATCAG
TAATCAAAATATTAATGAGTGCCTTACCTGAAGACATGATTTACATCTCTTGTATGCCCTCTACTTTAGCTCGTGATTTG
TCTATTTTGTGCGAAGATTTATATGAAATTCACGAGTGTACTGCGTATGATATGTTTCCACAAACTAGCCATGTCGAAAC
ACTTGTTCATCTTAAAAAAGTATAA

Upstream 100 bases:

>100_bases
TTAACAAATCCAGTATTCAATTACTCATTCGTGATTTCAAAGTTTTAGAAGAAGTGCCTACCACTTTTACTGAATTGGAA
CCCACTCAATATCAAAATTT

Downstream 100 bases:

>100_bases
CCTCTCAATTTTTTAAAAAAAAGAATTGAAAGGTTATAAAGTTGTTGCTCAATCAATGCATTTAGATTTTTTGAAGAAGA
ATGACTTAACTCAGCAATAA

Product: putative RNA methyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTHLLTIHTQVEGEITALAFGGAGILRYHGFVIFVPFTAPGDQIICRIIEIKKSFAVAELVKLKQPSSLRVQPPCPYFGK
CGGCQLQHLNEQTQLNYKLTAITDTLKRIGHLKVPSVEMNTAQLNWAYRRHITLHLKSFNQSFQAGYIATDHFSLISIDT
CPIFNNLHDPIISILQKFISTLPNPNQQDGRLTLLKNQNSQYILSFQFCSLNELNRILFQEALEKYPIFSGILVTSPKKQ
WIIGNPYSEQKIENLIFRFTPQAFIQNHPEQSLYIYKKICSLTIQLKSKKILDLYCGFGITSLLLASQGHFVTGIEYNSD
AIRFAKENSKLNHLPHVEFLEGDVEKILPICLKSQKCVDLVIVNPPRIGLSKSVIKILMSALPEDMIYISCMPSTLARDL
SILCEDLYEIHECTAYDMFPQTSHVETLVHLKKV

Sequences:

>Translated_434_residues
MTHLLTIHTQVEGEITALAFGGAGILRYHGFVIFVPFTAPGDQIICRIIEIKKSFAVAELVKLKQPSSLRVQPPCPYFGK
CGGCQLQHLNEQTQLNYKLTAITDTLKRIGHLKVPSVEMNTAQLNWAYRRHITLHLKSFNQSFQAGYIATDHFSLISIDT
CPIFNNLHDPIISILQKFISTLPNPNQQDGRLTLLKNQNSQYILSFQFCSLNELNRILFQEALEKYPIFSGILVTSPKKQ
WIIGNPYSEQKIENLIFRFTPQAFIQNHPEQSLYIYKKICSLTIQLKSKKILDLYCGFGITSLLLASQGHFVTGIEYNSD
AIRFAKENSKLNHLPHVEFLEGDVEKILPICLKSQKCVDLVIVNPPRIGLSKSVIKILMSALPEDMIYISCMPSTLARDL
SILCEDLYEIHECTAYDMFPQTSHVETLVHLKKV
>Mature_433_residues
THLLTIHTQVEGEITALAFGGAGILRYHGFVIFVPFTAPGDQIICRIIEIKKSFAVAELVKLKQPSSLRVQPPCPYFGKC
GGCQLQHLNEQTQLNYKLTAITDTLKRIGHLKVPSVEMNTAQLNWAYRRHITLHLKSFNQSFQAGYIATDHFSLISIDTC
PIFNNLHDPIISILQKFISTLPNPNQQDGRLTLLKNQNSQYILSFQFCSLNELNRILFQEALEKYPIFSGILVTSPKKQW
IIGNPYSEQKIENLIFRFTPQAFIQNHPEQSLYIYKKICSLTIQLKSKKILDLYCGFGITSLLLASQGHFVTGIEYNSDA
IRFAKENSKLNHLPHVEFLEGDVEKILPICLKSQKCVDLVIVNPPRIGLSKSVIKILMSALPEDMIYISCMPSTLARDLS
ILCEDLYEIHECTAYDMFPQTSHVETLVHLKKV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=225, Percent_Identity=28, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI51173878, Length=225, Percent_Identity=28, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI269784680, Length=201, Percent_Identity=28.8557213930348, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI269784676, Length=201, Percent_Identity=28.8557213930348, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI20149688, Length=201, Percent_Identity=28.8557213930348, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI269784678, Length=201, Percent_Identity=28.8557213930348, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789148, Length=419, Percent_Identity=26.9689737470167, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787083, Length=293, Percent_Identity=27.3037542662116, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790403, Length=188, Percent_Identity=29.7872340425532, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24666579, Length=207, Percent_Identity=28.5024154589372, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y248_PARUW (Q6MEM7)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007247.1
- ProteinModelPortal:   Q6MEM7
- STRING:   Q6MEM7
- GeneID:   2780239
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0248
- NMPDR:   fig|264201.1.peg.248
- eggNOG:   COG2265
- OMA:   IISINEC
- PhylomeDB:   Q6MEM7
- BioCyc:   CPRO264201:PC0248-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: NA

Molecular weight: Translated: 49268; Mature: 49137

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 391-391 BINDING 266-266 BINDING 295-295 BINDING 316-316 BINDING 364-364

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLLTIHTQVEGEITALAFGGAGILRYHGFVIFVPFTAPGDQIICRIIEIKKSFAVAEL
CCEEEEEEEEECCEEEEEEECCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VKLKQPSSLRVQPPCPYFGKCGGCQLQHLNEQTQLNYKLTAITDTLKRIGHLKVPSVEMN
HHHCCCCCCEECCCCCCCCCCCCCEEECCCCCHHEEEEEEHHHHHHHHHCCCCCCCEECC
TAQLNWAYRRHITLHLKSFNQSFQAGYIATDHFSLISIDTCPIFNNLHDPIISILQKFIS
CEEEEEEEEEEEEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHH
TLPNPNQQDGRLTLLKNQNSQYILSFQFCSLNELNRILFQEALEKYPIFSGILVTSPKKQ
HCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
WIIGNPYSEQKIENLIFRFTPQAFIQNHPEQSLYIYKKICSLTIQLKSKKILDLYCGFGI
EEECCCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHEEEEECCCEEEHHHHHHHH
TSLLLASQGHFVTGIEYNSDAIRFAKENSKLNHLPHVEFLEGDVEKILPICLKSQKCVDL
HHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCEEE
VIVNPPRIGLSKSVIKILMSALPEDMIYISCMPSTLARDLSILCEDLYEIHECTAYDMFP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHCCCC
QTSHVETLVHLKKV
CCHHHHHHHHHHCC
>Mature Secondary Structure 
THLLTIHTQVEGEITALAFGGAGILRYHGFVIFVPFTAPGDQIICRIIEIKKSFAVAEL
CEEEEEEEEECCEEEEEEECCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
VKLKQPSSLRVQPPCPYFGKCGGCQLQHLNEQTQLNYKLTAITDTLKRIGHLKVPSVEMN
HHHCCCCCCEECCCCCCCCCCCCCEEECCCCCHHEEEEEEHHHHHHHHHCCCCCCCEECC
TAQLNWAYRRHITLHLKSFNQSFQAGYIATDHFSLISIDTCPIFNNLHDPIISILQKFIS
CEEEEEEEEEEEEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHH
TLPNPNQQDGRLTLLKNQNSQYILSFQFCSLNELNRILFQEALEKYPIFSGILVTSPKKQ
HCCCCCCCCCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
WIIGNPYSEQKIENLIFRFTPQAFIQNHPEQSLYIYKKICSLTIQLKSKKILDLYCGFGI
EEECCCCCHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHEEEEECCCEEEHHHHHHHH
TSLLLASQGHFVTGIEYNSDAIRFAKENSKLNHLPHVEFLEGDVEKILPICLKSQKCVDL
HHHHHHCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCEEE
VIVNPPRIGLSKSVIKILMSALPEDMIYISCMPSTLARDLSILCEDLYEIHECTAYDMFP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHCCCC
QTSHVETLVHLKKV
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA