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The map label for this gene is recJ [H]

Identifier: 46445883

GI number: 46445883

Start: 349036

End: 350820

Strand: Reverse

Name: recJ [H]

Synonym: pc0249

Alternate gene names: 46445883

Gene position: 350820-349036 (Counterclockwise)

Preceding gene: 46445886

Following gene: 46445882

Centisome position: 14.53

GC content: 35.97

Gene sequence:

>1785_bases
ATGCATTTCACAACAAATGTCCAAGAGGATCCAATATGGGTTTATCCCCCAATGGACGAAGCATTAAAAGAAAGCATTGT
TAAAGAATTTAAACTTCACCCTGTTATAGCACAAATTTTGGTTTCCAGAGGATTTACTTCTTTTCAACAAATTCACGATT
ATTTATATGCTAAATTACCAGAATTGCACGATCCTTTTTTAATGGCAGAAATGCCTCAAGCGGTTGAACGAGTTTGTCGT
GCTATCCGTGATCATGAAAACATTCTTATCTATGGCGATAATGATGTTGATGGAATGACTGGAACTACTTTGTTGACCGA
ATTTCTACAAGATTTAGGCGCAAATGTTTTCTTCTATGTATCCAATAGAGGAGCGCTTCGACAAAGTTTAATCGTCGAAG
CTTTAGAATATGCTCTCATTAATAATTGCAAATTACTTATTACTGTAGATTGCGGTATCACCGCTGCTGTGGAAATAGCT
AAAGTAGCTGCACAAAACGTCGATGTGATTATCACTGATCACCACGAACCGACAGATAAAATTCCTCATTGTGTAGCGAC
TCTAAACCCAAAACTTGTTAACAACCCTTATCCGAATCGAGATTTAACAGGAGTTGGCGTCGCTTTTAAACTAGCTCACG
GAATCACTAATCAACTGACATCTGAAGGAAAAATTCCTCCTTCTAAGATTGATTTGAAAAAATATTTAGACTTGGTAGCA
TTAGGAACTATCTCTGATATGGGTTCATTGCTTGACACAGAAAATAGAATCTTAGTTCGCTATGGTCTTCGTCAGTTGCG
TAAAGGCAAACGAATGGGTCTAGCAAAACTTTTTTCTATTTGTGATGTCGATCTCAATGATTTAAATACATTTACCATAG
CCTCAAAAATTGCACCTCGTTTAAACAGTTTAGGACGTATTGCTGACCCTCGTAAAGGTGTCCAAATGTTACTTGTAAAA
AATGCTGATTTAGCAGAAAAGATGGCATTAGAGCTAGACCTTAATAATATCGAAAGGCAAAAAATTGAAAGAACAATGTC
TTCCGATGTAGATAGTACTATCCAACAAATACCTGCTATTTTATCTAACAAAGCAATTGTAATGGTTTCTGATAAATGGC
ATCCAGGTGTCATTGCCATACTATCGACACGAATTTCAAAACAATACAACAGACCGACTGTGATGATTGCTATCGATAAA
GGAATTGGAAAAGGTTCTATTCGCTCTATCCGCGAATTCCCCCTTCTACCAACTTTAAAAGAATGTTCAGATATTTTGAT
CAATTTCGGTGGACATGATTTCGCTGCAGGATTAACTATTAAAGAAGAGCATGTTGAACAATTTAAAAATCATTTTATTG
CTTCGGCTAATAAAAAGCTTGCTGATACAGATGTTATGAGTAAACTTTATCTTGATGCAGAAATTAAATTTAGTGAACTA
ACTTTTGATTTTATTGAATCAGCAAGGCTGTTAGAGCCATATGGAAATGAAAATCCTCAACCCATTTTGTATTGCGACGC
TTGGCAGACCTGGCCCCCAAAGATTGTTGGTAAAACGCATTTGAAGCTTTATTTAGAACAAGGAGATAGAGTTCTGGAAG
GTGTTGCACTTGGAAAAGCAACTTATAGCCAACAACTAAGGAAAAAGAATTTAAAACTGCGCATTGCTTTTACCCCTCAA
ATTAACAAATCCAGTATTCAATTACTCATTCGTGATTTCAAAGTTTTAGAAGAAGTGCCTACCACTTTTACTGAATTGGA
ACCCACTCAATATCAAAATTTATGA

Upstream 100 bases:

>100_bases
GAAAATGATTTAGATGAACGAACGAGATAAGTTATATACATGTGTATTAGCCAAACTCTAACGAAAAACACTCGATTTAT
AGAAACGGAGCTAGATTTTA

Downstream 100 bases:

>100_bases
CCCATCTATTGACTATTCATACTCAAGTTGAGGGAGAAATTACAGCGCTTGCTTTTGGAGGTGCAGGAATTTTAAGATAT
CATGGATTTGTAATTTTTGT

Product: putative ssDNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MHFTTNVQEDPIWVYPPMDEALKESIVKEFKLHPVIAQILVSRGFTSFQQIHDYLYAKLPELHDPFLMAEMPQAVERVCR
AIRDHENILIYGDNDVDGMTGTTLLTEFLQDLGANVFFYVSNRGALRQSLIVEALEYALINNCKLLITVDCGITAAVEIA
KVAAQNVDVIITDHHEPTDKIPHCVATLNPKLVNNPYPNRDLTGVGVAFKLAHGITNQLTSEGKIPPSKIDLKKYLDLVA
LGTISDMGSLLDTENRILVRYGLRQLRKGKRMGLAKLFSICDVDLNDLNTFTIASKIAPRLNSLGRIADPRKGVQMLLVK
NADLAEKMALELDLNNIERQKIERTMSSDVDSTIQQIPAILSNKAIVMVSDKWHPGVIAILSTRISKQYNRPTVMIAIDK
GIGKGSIRSIREFPLLPTLKECSDILINFGGHDFAAGLTIKEEHVEQFKNHFIASANKKLADTDVMSKLYLDAEIKFSEL
TFDFIESARLLEPYGNENPQPILYCDAWQTWPPKIVGKTHLKLYLEQGDRVLEGVALGKATYSQQLRKKNLKLRIAFTPQ
INKSSIQLLIRDFKVLEEVPTTFTELEPTQYQNL

Sequences:

>Translated_594_residues
MHFTTNVQEDPIWVYPPMDEALKESIVKEFKLHPVIAQILVSRGFTSFQQIHDYLYAKLPELHDPFLMAEMPQAVERVCR
AIRDHENILIYGDNDVDGMTGTTLLTEFLQDLGANVFFYVSNRGALRQSLIVEALEYALINNCKLLITVDCGITAAVEIA
KVAAQNVDVIITDHHEPTDKIPHCVATLNPKLVNNPYPNRDLTGVGVAFKLAHGITNQLTSEGKIPPSKIDLKKYLDLVA
LGTISDMGSLLDTENRILVRYGLRQLRKGKRMGLAKLFSICDVDLNDLNTFTIASKIAPRLNSLGRIADPRKGVQMLLVK
NADLAEKMALELDLNNIERQKIERTMSSDVDSTIQQIPAILSNKAIVMVSDKWHPGVIAILSTRISKQYNRPTVMIAIDK
GIGKGSIRSIREFPLLPTLKECSDILINFGGHDFAAGLTIKEEHVEQFKNHFIASANKKLADTDVMSKLYLDAEIKFSEL
TFDFIESARLLEPYGNENPQPILYCDAWQTWPPKIVGKTHLKLYLEQGDRVLEGVALGKATYSQQLRKKNLKLRIAFTPQ
INKSSIQLLIRDFKVLEEVPTTFTELEPTQYQNL
>Mature_594_residues
MHFTTNVQEDPIWVYPPMDEALKESIVKEFKLHPVIAQILVSRGFTSFQQIHDYLYAKLPELHDPFLMAEMPQAVERVCR
AIRDHENILIYGDNDVDGMTGTTLLTEFLQDLGANVFFYVSNRGALRQSLIVEALEYALINNCKLLITVDCGITAAVEIA
KVAAQNVDVIITDHHEPTDKIPHCVATLNPKLVNNPYPNRDLTGVGVAFKLAHGITNQLTSEGKIPPSKIDLKKYLDLVA
LGTISDMGSLLDTENRILVRYGLRQLRKGKRMGLAKLFSICDVDLNDLNTFTIASKIAPRLNSLGRIADPRKGVQMLLVK
NADLAEKMALELDLNNIERQKIERTMSSDVDSTIQQIPAILSNKAIVMVSDKWHPGVIAILSTRISKQYNRPTVMIAIDK
GIGKGSIRSIREFPLLPTLKECSDILINFGGHDFAAGLTIKEEHVEQFKNHFIASANKKLADTDVMSKLYLDAEIKFSEL
TFDFIESARLLEPYGNENPQPILYCDAWQTWPPKIVGKTHLKLYLEQGDRVLEGVALGKATYSQQLRKKNLKLRIAFTPQ
INKSSIQLLIRDFKVLEEVPTTFTELEPTQYQNL

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=557, Percent_Identity=29.9820466786355, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 66787; Mature: 66787

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFTTNVQEDPIWVYPPMDEALKESIVKEFKLHPVIAQILVSRGFTSFQQIHDYLYAKLP
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ELHDPFLMAEMPQAVERVCRAIRDHENILIYGDNDVDGMTGTTLLTEFLQDLGANVFFYV
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEE
SNRGALRQSLIVEALEYALINNCKLLITVDCGITAAVEIAKVAAQNVDVIITDHHEPTDK
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCHH
IPHCVATLNPKLVNNPYPNRDLTGVGVAFKLAHGITNQLTSEGKIPPSKIDLKKYLDLVA
HCHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGTISDMGSLLDTENRILVRYGLRQLRKGKRMGLAKLFSICDVDLNDLNTFTIASKIAPR
HHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LNSLGRIADPRKGVQMLLVKNADLAEKMALELDLNNIERQKIERTMSSDVDSTIQQIPAI
HHHCCCCCCCCCCCEEEEEECCCHHHHHEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHH
LSNKAIVMVSDKWHPGVIAILSTRISKQYNRPTVMIAIDKGIGKGSIRSIREFPLLPTLK
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCHH
ECSDILINFGGHDFAAGLTIKEEHVEQFKNHFIASANKKLADTDVMSKLYLDAEIKFSEL
HHHHHEEECCCCCEECCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHH
TFDFIESARLLEPYGNENPQPILYCDAWQTWPPKIVGKTHLKLYLEQGDRVLEGVALGKA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHEEEEEHHCCHHHHHHHHHHH
TYSQQLRKKNLKLRIAFTPQINKSSIQLLIRDFKVLEEVPTTFTELEPTQYQNL
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCCC
>Mature Secondary Structure
MHFTTNVQEDPIWVYPPMDEALKESIVKEFKLHPVIAQILVSRGFTSFQQIHDYLYAKLP
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
ELHDPFLMAEMPQAVERVCRAIRDHENILIYGDNDVDGMTGTTLLTEFLQDLGANVFFYV
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEE
SNRGALRQSLIVEALEYALINNCKLLITVDCGITAAVEIAKVAAQNVDVIITDHHEPTDK
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCHH
IPHCVATLNPKLVNNPYPNRDLTGVGVAFKLAHGITNQLTSEGKIPPSKIDLKKYLDLVA
HCHHHHHCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGTISDMGSLLDTENRILVRYGLRQLRKGKRMGLAKLFSICDVDLNDLNTFTIASKIAPR
HHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LNSLGRIADPRKGVQMLLVKNADLAEKMALELDLNNIERQKIERTMSSDVDSTIQQIPAI
HHHCCCCCCCCCCCEEEEEECCCHHHHHEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHH
LSNKAIVMVSDKWHPGVIAILSTRISKQYNRPTVMIAIDKGIGKGSIRSIREFPLLPTLK
HCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCHH
ECSDILINFGGHDFAAGLTIKEEHVEQFKNHFIASANKKLADTDVMSKLYLDAEIKFSEL
HHHHHEEECCCCCEECCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEHHHH
TFDFIESARLLEPYGNENPQPILYCDAWQTWPPKIVGKTHLKLYLEQGDRVLEGVALGKA
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCHHEEEEEHHCCHHHHHHHHHHH
TYSQQLRKKNLKLRIAFTPQINKSSIQLLIRDFKVLEEVPTTFTELEPTQYQNL
HHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]