Click here to switch to the map view.

The map label for this gene is lipA

Identifier: 46445786

GI number: 46445786

Start: 228912

End: 229883

Strand: Direct

Name: lipA

Synonym: pc0152

Alternate gene names: 46445786

Gene position: 228912-229883 (Clockwise)

Preceding gene: 46445785

Following gene: 46445787

Centisome position: 9.48

GC content: 37.24

Gene sequence:

>972_bases
GTGATGGAAAACTCTCCAAAAACTCGTCGCTTAAATATCCTACCAGATAATCCTGAAAATACTGGAGATGGTGTGGTAGG
GCTTGGCCGCTTTCCTTCATGGTTGCACCGGCCACTTCCTAAAGGAAACCAACTTCAAATTACTGGGCAAGTCATCAACC
AAAATCGCTTACACACTGTTTGCGAAGAGGCTAAATGTCCCAATCTCTTAGAATGTTGGACTAAAAAAACTGCCACATTT
CTTGTAATGGGTAAAGAGTGTTCACGTAACTGTGGTTTTTGTGATATAGACTTTTCAAAAAATCCTAAACCCCTAGATCG
AAGTGAACCGTCGAGAGTTGCTTTATCTGTCCAACAGTTAGGATTAAAGCACGTTGTAATCACAATGGTAGCACGAGATG
ATTTGTCTGATGGAGGAAGTTCTCATCTCGTCGAGGTCATTGAGGCGATTAGGCAAACTAATGAAGATGTAACTATTGAG
GTCTTAACTTCGGATTTTGAAGGAAATCGAAAGGCTCTAAGCTTTGTTTTACAAGCTAAACCTGAAATTTTTAATCATAA
TATTGAAACAGTTCGCCGTTTAACTCCACGTGTTAGACATAAAGCCACTTATGAAAGAACTTTAAGTGTACTTGAACAAG
CTGCCCAAAAGAAATATCACTCCCAACTTAAGGTTAAATCAGGGATAATGGTGGGGTTAGGTGAGACTGAAGAAGAAATT
TTTGAAACATTATTAGATTTAAAAAGGGTAGGATGTGAAATTGTCACCATTGGACAGTATTTACAACCTAATCGGCAAAA
ACTCCTTGTAAAATCTTTTGTTCATCCTGATATATTTAAAAAATATGAACAATATGGATTGAGTATAGGAATACCTCATC
TTTATTGTGGACCTTTTGTTAGATCTAGCTATAACGCAAATTTAGTTCTTATGCGTGCAAATCAAAAAGAAGCAATTGTT
AACAGCGAATAG

Upstream 100 bases:

>100_bases
AAGCTTGGCTAGAATCAGCACTACTAGGGGAAGGTTTACCCTTGCATTTACCCCCTAAAACGCAAAAAAAGGGATAAGAT
TCAATTTTTAAAGGACTGCT

Downstream 100 bases:

>100_bases
TAATTAGGTGAATGATGCAAGAATCAGGCGAACCATTTGAACATTATCCTTTAGTAGATGCAATGGATCATGAAATTTTG
ATGCATCGAGACGCTCATTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MMENSPKTRRLNILPDNPENTGDGVVGLGRFPSWLHRPLPKGNQLQITGQVINQNRLHTVCEEAKCPNLLECWTKKTATF
LVMGKECSRNCGFCDIDFSKNPKPLDRSEPSRVALSVQQLGLKHVVITMVARDDLSDGGSSHLVEVIEAIRQTNEDVTIE
VLTSDFEGNRKALSFVLQAKPEIFNHNIETVRRLTPRVRHKATYERTLSVLEQAAQKKYHSQLKVKSGIMVGLGETEEEI
FETLLDLKRVGCEIVTIGQYLQPNRQKLLVKSFVHPDIFKKYEQYGLSIGIPHLYCGPFVRSSYNANLVLMRANQKEAIV
NSE

Sequences:

>Translated_323_residues
MMENSPKTRRLNILPDNPENTGDGVVGLGRFPSWLHRPLPKGNQLQITGQVINQNRLHTVCEEAKCPNLLECWTKKTATF
LVMGKECSRNCGFCDIDFSKNPKPLDRSEPSRVALSVQQLGLKHVVITMVARDDLSDGGSSHLVEVIEAIRQTNEDVTIE
VLTSDFEGNRKALSFVLQAKPEIFNHNIETVRRLTPRVRHKATYERTLSVLEQAAQKKYHSQLKVKSGIMVGLGETEEEI
FETLLDLKRVGCEIVTIGQYLQPNRQKLLVKSFVHPDIFKKYEQYGLSIGIPHLYCGPFVRSSYNANLVLMRANQKEAIV
NSE
>Mature_323_residues
MMENSPKTRRLNILPDNPENTGDGVVGLGRFPSWLHRPLPKGNQLQITGQVINQNRLHTVCEEAKCPNLLECWTKKTATF
LVMGKECSRNCGFCDIDFSKNPKPLDRSEPSRVALSVQQLGLKHVVITMVARDDLSDGGSSHLVEVIEAIRQTNEDVTIE
VLTSDFEGNRKALSFVLQAKPEIFNHNIETVRRLTPRVRHKATYERTLSVLEQAAQKKYHSQLKVKSGIMVGLGETEEEI
FETLLDLKRVGCEIVTIGQYLQPNRQKLLVKSFVHPDIFKKYEQYGLSIGIPHLYCGPFVRSSYNANLVLMRANQKEAIV
NSE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=289, Percent_Identity=38.0622837370242, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=38.8663967611336, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1786846, Length=284, Percent_Identity=44.3661971830986, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=39.0243902439024, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=283, Percent_Identity=40.9893992932862, Blast_Score=241, Evalue=8e-65,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=42.4028268551237, Blast_Score=244, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PARUW (Q6MEX3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007151.1
- ProteinModelPortal:   Q6MEX3
- STRING:   Q6MEX3
- GeneID:   2780836
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0152
- NMPDR:   fig|264201.1.peg.152
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   DPHEPQR
- PhylomeDB:   Q6MEX3
- ProtClustDB:   PRK05481
- BioCyc:   CPRO264201:PC0152-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36511; Mature: 36511

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENSPKTRRLNILPDNPENTGDGVVGLGRFPSWLHRPLPKGNQLQITGQVINQNRLHTV
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHH
CEEAKCPNLLECWTKKTATFLVMGKECSRNCGFCDIDFSKNPKPLDRSEPSRVALSVQQL
HHHCCCCCHHHHHCCCCEEEEEECHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
GLKHVVITMVARDDLSDGGSSHLVEVIEAIRQTNEDVTIEVLTSDFEGNRKALSFVLQAK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCC
PEIFNHNIETVRRLTPRVRHKATYERTLSVLEQAAQKKYHSQLKVKSGIMVGLGETEEEI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHH
FETLLDLKRVGCEIVTIGQYLQPNRQKLLVKSFVHPDIFKKYEQYGLSIGIPHLYCGPFV
HHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCHHHHCHHH
RSSYNANLVLMRANQKEAIVNSE
HCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MMENSPKTRRLNILPDNPENTGDGVVGLGRFPSWLHRPLPKGNQLQITGQVINQNRLHTV
CCCCCCCCEEEEECCCCCCCCCCCEEECCCCHHHHHCCCCCCCEEEEEEEECCCCHHHHH
CEEAKCPNLLECWTKKTATFLVMGKECSRNCGFCDIDFSKNPKPLDRSEPSRVALSVQQL
HHHCCCCCHHHHHCCCCEEEEEECHHHCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHH
GLKHVVITMVARDDLSDGGSSHLVEVIEAIRQTNEDVTIEVLTSDFEGNRKALSFVLQAK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCC
PEIFNHNIETVRRLTPRVRHKATYERTLSVLEQAAQKKYHSQLKVKSGIMVGLGETEEEI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHH
FETLLDLKRVGCEIVTIGQYLQPNRQKLLVKSFVHPDIFKKYEQYGLSIGIPHLYCGPFV
HHHHHHHHHCCCEEEEECHHHCCCHHHHHHHHHCCHHHHHHHHHCCCEECCCHHHHCHHH
RSSYNANLVLMRANQKEAIVNSE
HCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA