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The map label for this gene is emrA [H]

Identifier: 46445762

GI number: 46445762

Start: 198476

End: 199675

Strand: Reverse

Name: emrA [H]

Synonym: pc0128

Alternate gene names: 46445762

Gene position: 199675-198476 (Counterclockwise)

Preceding gene: 46445763

Following gene: 46445761

Centisome position: 8.27

GC content: 34.25

Gene sequence:

>1200_bases
ATGACTGAAAAAGAACCTACAACTACTAATCAAGATTTTTCTTCTCTTAATCTAGATCTTCTCAATAAGAAAAAAAGAAA
TAAAGTTTTATTAAAGATTTTTTTTGTTTTTTTATTATTAACTATTCTAACAACTATTTATTGGTATTTGTTCATCCGTT
TTCATGAATCGACAGATGATGCTTATGTCAATGGTAACATTGTTAATTTGATGTCTCCTGAACAAGGAACCATTACAGCT
ATTTATGCAGATAATACAGATTTTGTAAAACAAGGCCAGCTACTGATAGAACTTGATCCAACACTCTATCAATTAGCTTT
TGAACAAGCTCAAGTTGAGCTAGCTTTAGCTGTCAGGGAAGTTCAACAACTTTATGAAACTGTCCAACAAAAAAAAGCCG
CTCTTGAATTAAAACAAGCCGATTTAAAACGAGCCCAAGAAGATTTTGAAAGTCGTAATTCCTTAAGAAATACTGAAGCT
ATTTCGATTGAAGATCTCAATCATGCACAAGCTAATTTAACTGTTGCACAAGCTGCAACGAACTTATCAAAACATGAATT
AAATAGCGCCATTGCTGCTATAGGCTCAACATCATTGGAGAATCATCCCAATATTCAGAATAAAGAAATTGCACTGAAGC
AAACCTATGTCAATTTAAAGCGATGCAAAATTTTAGCTCCCGTCTCAGGTTTTATTTCTCAAAGAAAAATACAAGTAGGA
GAATGGGTCTCAACAACACGCTCTCTATTGAGTATTATTCCTCTCGATCAAATTTGGATAGATGCCAATTTCAAAGAAAC
AGAATTGCGTGATATTCGAATAGGACAACCCGTCACTGTTAAAAGTGACATGTATGGTTCTTCCGTGCTTTATCATGGTA
AAGTATTAGGAGTATTAGCTGGAACTGGAAGTGTTTTTTCTTTAATACCTCCTCAAAATGCTACTGGAAACTGGATCAAA
ATTGTTCAACGTGTTCCAGTTAGAATTTCTCTTGATTCTAACGAACTTAGACAATTCCCCCTGTTTTTAGGTCTATCCAC
CTATGTTGTTGTCGACACGAAAAATCAACTAGGCGATCGATTAGCCTCCAATCCTGTTTACAACCCCATACTTACAACTT
CTGTTTATGAAATTCCTATGCAGCCTGCTCAAGAACTTATTGATAGTATTATTCAAGCTAATTTAAACCACAAATCTTGA

Upstream 100 bases:

>100_bases
AGCTCTCAAAATCCTAAACATCCTAATTTGAACATTAGTCAGCTACAAATTTATTTAGAAATTTTACCCCTTTCTTAAGA
AGATATAAGATAATAAAAAC

Downstream 100 bases:

>100_bases
TATGCCAGTTAAAACTTTAAGCCCAGCGGCATTAATTATTTTAACACTTGCATTAGGTCTTGGAACATTTATTCAGGTTT
TAGACAGTTCAATTGCTAAC

Product: multidrug resistance protein, emrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTEKEPTTTNQDFSSLNLDLLNKKKRNKVLLKIFFVFLLLTILTTIYWYLFIRFHESTDDAYVNGNIVNLMSPEQGTITA
IYADNTDFVKQGQLLIELDPTLYQLAFEQAQVELALAVREVQQLYETVQQKKAALELKQADLKRAQEDFESRNSLRNTEA
ISIEDLNHAQANLTVAQAATNLSKHELNSAIAAIGSTSLENHPNIQNKEIALKQTYVNLKRCKILAPVSGFISQRKIQVG
EWVSTTRSLLSIIPLDQIWIDANFKETELRDIRIGQPVTVKSDMYGSSVLYHGKVLGVLAGTGSVFSLIPPQNATGNWIK
IVQRVPVRISLDSNELRQFPLFLGLSTYVVVDTKNQLGDRLASNPVYNPILTTSVYEIPMQPAQELIDSIIQANLNHKS

Sequences:

>Translated_399_residues
MTEKEPTTTNQDFSSLNLDLLNKKKRNKVLLKIFFVFLLLTILTTIYWYLFIRFHESTDDAYVNGNIVNLMSPEQGTITA
IYADNTDFVKQGQLLIELDPTLYQLAFEQAQVELALAVREVQQLYETVQQKKAALELKQADLKRAQEDFESRNSLRNTEA
ISIEDLNHAQANLTVAQAATNLSKHELNSAIAAIGSTSLENHPNIQNKEIALKQTYVNLKRCKILAPVSGFISQRKIQVG
EWVSTTRSLLSIIPLDQIWIDANFKETELRDIRIGQPVTVKSDMYGSSVLYHGKVLGVLAGTGSVFSLIPPQNATGNWIK
IVQRVPVRISLDSNELRQFPLFLGLSTYVVVDTKNQLGDRLASNPVYNPILTTSVYEIPMQPAQELIDSIIQANLNHKS
>Mature_398_residues
TEKEPTTTNQDFSSLNLDLLNKKKRNKVLLKIFFVFLLLTILTTIYWYLFIRFHESTDDAYVNGNIVNLMSPEQGTITAI
YADNTDFVKQGQLLIELDPTLYQLAFEQAQVELALAVREVQQLYETVQQKKAALELKQADLKRAQEDFESRNSLRNTEAI
SIEDLNHAQANLTVAQAATNLSKHELNSAIAAIGSTSLENHPNIQNKEIALKQTYVNLKRCKILAPVSGFISQRKIQVGE
WVSTTRSLLSIIPLDQIWIDANFKETELRDIRIGQPVTVKSDMYGSSVLYHGKVLGVLAGTGSVFSLIPPQNATGNWIKI
VQRVPVRISLDSNELRQFPLFLGLSTYVVVDTKNQLGDRLASNPVYNPILTTSVYEIPMQPAQELIDSIIQANLNHKS

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=370, Percent_Identity=43.7837837837838, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788711, Length=383, Percent_Identity=39.1644908616188, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI1790519, Length=296, Percent_Identity=28.0405405405405, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87081951, Length=309, Percent_Identity=24.5954692556634, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789637, Length=327, Percent_Identity=23.2415902140673, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44860; Mature: 44729

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKEPTTTNQDFSSLNLDLLNKKKRNKVLLKIFFVFLLLTILTTIYWYLFIRFHESTDD
CCCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
AYVNGNIVNLMSPEQGTITAIYADNTDFVKQGQLLIELDPTLYQLAFEQAQVELALAVRE
EEECCCEEEEECCCCCCEEEEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
VQQLYETVQQKKAALELKQADLKRAQEDFESRNSLRNTEAISIEDLNHAQANLTVAQAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCEEHHHHHH
NLSKHELNSAIAAIGSTSLENHPNIQNKEIALKQTYVNLKRCKILAPVSGFISQRKIQVG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHEEECCHHHHHHHHHEHHH
EWVSTTRSLLSIIPLDQIWIDANFKETELRDIRIGQPVTVKSDMYGSSVLYHGKVLGVLA
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCEEEECCEEEEEEE
GTGSVFSLIPPQNATGNWIKIVQRVPVRISLDSNELRQFPLFLGLSTYVVVDTKNQLGDR
CCCCEEEEECCCCCCCHHHHHHHHCCEEEEECHHHHHHHHHEECCEEEEEEECCHHHHHH
LASNPVYNPILTTSVYEIPMQPAQELIDSIIQANLNHKS
HHCCCCCCCHHHHHEEECCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEKEPTTTNQDFSSLNLDLLNKKKRNKVLLKIFFVFLLLTILTTIYWYLFIRFHESTDD
CCCCCCCCCCCHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCC
AYVNGNIVNLMSPEQGTITAIYADNTDFVKQGQLLIELDPTLYQLAFEQAQVELALAVRE
EEECCCEEEEECCCCCCEEEEEECCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
VQQLYETVQQKKAALELKQADLKRAQEDFESRNSLRNTEAISIEDLNHAQANLTVAQAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHCEEHHHHHH
NLSKHELNSAIAAIGSTSLENHPNIQNKEIALKQTYVNLKRCKILAPVSGFISQRKIQVG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHEEECCHHHHHHHHHEHHH
EWVSTTRSLLSIIPLDQIWIDANFKETELRDIRIGQPVTVKSDMYGSSVLYHGKVLGVLA
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCEEEECCEEEEEEE
GTGSVFSLIPPQNATGNWIKIVQRVPVRISLDSNELRQFPLFLGLSTYVVVDTKNQLGDR
CCCCEEEEECCCCCCCHHHHHHHHCCEEEEECHHHHHHHHHEECCEEEEEEECCHHHHHH
LASNPVYNPILTTSVYEIPMQPAQELIDSIIQANLNHKS
HHCCCCCCCHHHHHEEECCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]