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The map label for this gene is emrB [H]

Identifier: 46445761

GI number: 46445761

Start: 196933

End: 198474

Strand: Reverse

Name: emrB [H]

Synonym: pc0127

Alternate gene names: 46445761

Gene position: 198474-196933 (Counterclockwise)

Preceding gene: 46445762

Following gene: 46445755

Centisome position: 8.22

GC content: 36.58

Gene sequence:

>1542_bases
ATGCCAGTTAAAACTTTAAGCCCAGCGGCATTAATTATTTTAACACTTGCATTAGGTCTTGGAACATTTATTCAGGTTTT
AGACAGTTCAATTGCTAACGTATCAATTTCTCACATTGCCGGTGATTTAGCAGTTAGCCCTAACCAAGGAACATGGGTAA
TTACTTCATTTGCCATTAGCAACGGAATCGTTTTAGCTATCACAGGTTGGTTAGCAGCTCGTTTTGGACGGGTTCGTTTA
TTCATTTGGTCCACTAGCCTTTTTTCAATTACTTCATGGCTTTGTGGTTTGGCTTGGGATTTGAATGTACTTATTCTTTT
TCGAACATTACAGGGAGCATCTGCTGGAGCTTTAATTCCTTTATCACAAGCTTTATTGTTAACAAATTATCCGGAAGAAA
AAAAAGGAGTCGCACTTGGCTTTTGGTCGATGATCGTTATTGTAGCCCCTATTTTAGGACCAATTGTAGGAGGATATATT
ACAGATAATTATGGTTGGCCCTGGATTTTTTATATTAACATTCCCATTGGTTTTTTATCGGCTTGGATGACAGGTCTTTT
AATTCAAGATCCTCCTCACGAAGGAAAATCTAAAGTTCCTCTTGACCTGACTGGTCTTTTACTTTTAACCATTGGCATTA
GCTCATTGCAAGTTTTTTTAGATAAAGGGCAAGAATTAGACTGGTTAAACTCTTTGACTATTCGTGTCCTTATCGTGACT
TGCGTAATCGCTTTTACAATTTTTATTCCATGGAACTATTATGCGAAACATCCAGTCATGAATTTTTCTTATTTTAGACA
TCGTAACTTTACAATTGGAACAATTTTGGGAGCGATAGGATTTCTCATGTTTTTTGGAAGCGCTGTCTTACTTCCCCTTT
GGTTAGAAACAGAAATGGGTTACACGGCTTTTTGGGCAGGATTTGCTGTTATGCCAATTGGAATAATTCCTCTATTTTTA
TCTTCTACAATCGGAAAAAGTGTTAGTCGATTTGATCCAAGAATCATGACAACATTTAGTTTTATCAGTTTTGGACTAAC
ATTCTTTTGGTTTAGTGAATTGACCCCTTCTGTCAGTTTATTTAAATTGATGCTTCCTCGCTTTTTTCAAGGAATCGGTC
TCAGCTTCTTTTTTATCCCTCTTGTAACAATTTCATTGTCTCCCATTCCTAATCACGAATTAGCGGGCGCCTCAGGAATC
TTTAATTTTATTCGATTAATTGCTGGAGGAGGATTCGGAACGGCTTTGTATGTTACTTTATGGGATCGTAGAGAAATTTT
TCACCATAGCCGTTTAACAGAAGCAACGACAATCTATGACCCTTTAACATGGGAATTTTACGAAAATTTAAAACAATCAC
TTAGCTTAAGTCAACCTGAATCCAATCTTATTCTGGAAGATTTATTGTCCGCTCAATCGTATTTATTAGCTGTTAATGAT
GTCTTTTGGCTAACAGGATGGGTGTTCTTGGCCTGCATTCCTTTAATTTGGTTATGCAAAAAAAATACCACAAATAAAAC
TCAGCAAGTCGCCATGGATTAA

Upstream 100 bases:

>100_bases
AACCCCATACTTACAACTTCTGTTTATGAAATTCCTATGCAGCCTGCTCAAGAACTTATTGATAGTATTATTCAAGCTAA
TTTAAACCACAAATCTTGAT

Downstream 100 bases:

>100_bases
TGGGATCTCTTTCGAAAAAAAGTCTCTTCATTTAACGATCGGCGTAATTAAGCTCTATCCATCTTCGATAACTACCCGTT
TGGATATTTTTTACCCATTG

Product: putative multidrug resistance membrane translocase protein, emrB

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPVKTLSPAALIILTLALGLGTFIQVLDSSIANVSISHIAGDLAVSPNQGTWVITSFAISNGIVLAITGWLAARFGRVRL
FIWSTSLFSITSWLCGLAWDLNVLILFRTLQGASAGALIPLSQALLLTNYPEEKKGVALGFWSMIVIVAPILGPIVGGYI
TDNYGWPWIFYINIPIGFLSAWMTGLLIQDPPHEGKSKVPLDLTGLLLLTIGISSLQVFLDKGQELDWLNSLTIRVLIVT
CVIAFTIFIPWNYYAKHPVMNFSYFRHRNFTIGTILGAIGFLMFFGSAVLLPLWLETEMGYTAFWAGFAVMPIGIIPLFL
SSTIGKSVSRFDPRIMTTFSFISFGLTFFWFSELTPSVSLFKLMLPRFFQGIGLSFFFIPLVTISLSPIPNHELAGASGI
FNFIRLIAGGGFGTALYVTLWDRREIFHHSRLTEATTIYDPLTWEFYENLKQSLSLSQPESNLILEDLLSAQSYLLAVND
VFWLTGWVFLACIPLIWLCKKNTTNKTQQVAMD

Sequences:

>Translated_513_residues
MPVKTLSPAALIILTLALGLGTFIQVLDSSIANVSISHIAGDLAVSPNQGTWVITSFAISNGIVLAITGWLAARFGRVRL
FIWSTSLFSITSWLCGLAWDLNVLILFRTLQGASAGALIPLSQALLLTNYPEEKKGVALGFWSMIVIVAPILGPIVGGYI
TDNYGWPWIFYINIPIGFLSAWMTGLLIQDPPHEGKSKVPLDLTGLLLLTIGISSLQVFLDKGQELDWLNSLTIRVLIVT
CVIAFTIFIPWNYYAKHPVMNFSYFRHRNFTIGTILGAIGFLMFFGSAVLLPLWLETEMGYTAFWAGFAVMPIGIIPLFL
SSTIGKSVSRFDPRIMTTFSFISFGLTFFWFSELTPSVSLFKLMLPRFFQGIGLSFFFIPLVTISLSPIPNHELAGASGI
FNFIRLIAGGGFGTALYVTLWDRREIFHHSRLTEATTIYDPLTWEFYENLKQSLSLSQPESNLILEDLLSAQSYLLAVND
VFWLTGWVFLACIPLIWLCKKNTTNKTQQVAMD
>Mature_512_residues
PVKTLSPAALIILTLALGLGTFIQVLDSSIANVSISHIAGDLAVSPNQGTWVITSFAISNGIVLAITGWLAARFGRVRLF
IWSTSLFSITSWLCGLAWDLNVLILFRTLQGASAGALIPLSQALLLTNYPEEKKGVALGFWSMIVIVAPILGPIVGGYIT
DNYGWPWIFYINIPIGFLSAWMTGLLIQDPPHEGKSKVPLDLTGLLLLTIGISSLQVFLDKGQELDWLNSLTIRVLIVTC
VIAFTIFIPWNYYAKHPVMNFSYFRHRNFTIGTILGAIGFLMFFGSAVLLPLWLETEMGYTAFWAGFAVMPIGIIPLFLS
STIGKSVSRFDPRIMTTFSFISFGLTFFWFSELTPSVSLFKLMLPRFFQGIGLSFFFIPLVTISLSPIPNHELAGASGIF
NFIRLIAGGGFGTALYVTLWDRREIFHHSRLTEATTIYDPLTWEFYENLKQSLSLSQPESNLILEDLLSAQSYLLAVNDV
FWLTGWVFLACIPLIWLCKKNTTNKTQQVAMD

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=45.7831325301205, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1788710, Length=515, Percent_Identity=42.5242718446602, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1790195, Length=386, Percent_Identity=30.5699481865285, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1788392, Length=389, Percent_Identity=28.7917737789203, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081983, Length=416, Percent_Identity=26.2019230769231, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=167, Percent_Identity=31.7365269461078, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=286, Percent_Identity=23.7762237762238, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=129, Percent_Identity=28.6821705426357, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56994; Mature: 56863

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKTLSPAALIILTLALGLGTFIQVLDSSIANVSISHIAGDLAVSPNQGTWVITSFAIS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHCCCEEECCCCCCEEEEEEEHH
NGIVLAITGWLAARFGRVRLFIWSTSLFSITSWLCGLAWDLNVLILFRTLQGASAGALIP
CCEEEEEHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCCCHH
LSQALLLTNYPEEKKGVALGFWSMIVIVAPILGPIVGGYITDNYGWPWIFYINIPIGFLS
HHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCHHHHH
AWMTGLLIQDPPHEGKSKVPLDLTGLLLLTIGISSLQVFLDKGQELDWLNSLTIRVLIVT
HHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CVIAFTIFIPWNYYAKHPVMNFSYFRHRNFTIGTILGAIGFLMFFGSAVLLPLWLETEMG
HHHHHHHHCCCCHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
YTAFWAGFAVMPIGIIPLFLSSTIGKSVSRFDPRIMTTFSFISFGLTFFWFSELTPSVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
FKLMLPRFFQGIGLSFFFIPLVTISLSPIPNHELAGASGIFNFIRLIAGGGFGTALYVTL
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
WDRREIFHHSRLTEATTIYDPLTWEFYENLKQSLSLSQPESNLILEDLLSAQSYLLAVND
ECHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VFWLTGWVFLACIPLIWLCKKNTTNKTQQVAMD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
PVKTLSPAALIILTLALGLGTFIQVLDSSIANVSISHIAGDLAVSPNQGTWVITSFAIS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHCCCEEECCCCCCEEEEEEEHH
NGIVLAITGWLAARFGRVRLFIWSTSLFSITSWLCGLAWDLNVLILFRTLQGASAGALIP
CCEEEEEHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEHHCCCCCCCCHH
LSQALLLTNYPEEKKGVALGFWSMIVIVAPILGPIVGGYITDNYGWPWIFYINIPIGFLS
HHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEECCHHHHH
AWMTGLLIQDPPHEGKSKVPLDLTGLLLLTIGISSLQVFLDKGQELDWLNSLTIRVLIVT
HHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
CVIAFTIFIPWNYYAKHPVMNFSYFRHRNFTIGTILGAIGFLMFFGSAVLLPLWLETEMG
HHHHHHHHCCCCHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
YTAFWAGFAVMPIGIIPLFLSSTIGKSVSRFDPRIMTTFSFISFGLTFFWFSELTPSVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHH
FKLMLPRFFQGIGLSFFFIPLVTISLSPIPNHELAGASGIFNFIRLIAGGGFGTALYVTL
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEE
WDRREIFHHSRLTEATTIYDPLTWEFYENLKQSLSLSQPESNLILEDLLSAQSYLLAVND
ECHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VFWLTGWVFLACIPLIWLCKKNTTNKTQQVAMD
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]