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The map label for this gene is rfbA [H]

Identifier: 46445757

GI number: 46445757

Start: 193531

End: 194418

Strand: Direct

Name: rfbA [H]

Synonym: pc0123

Alternate gene names: 46445757

Gene position: 193531-194418 (Clockwise)

Preceding gene: 46445756

Following gene: 46445758

Centisome position: 8.02

GC content: 37.84

Gene sequence:

>888_bases
ATGAAAGGAATTGTTCTAGCCGGAGGAAGTGGCACAAGACTGCATCCATTAACATTGGGAGTGACTAAACAGCTACTGCC
AGTTTACAACAAACCGATGATTTATTATCCCCTTTCAGTTTTATTATTAGCCGAAATAAAAGATATCTTGATCATTTCCA
CTCCAGAAGATATGCCTGTTTTTAAACGACTGCTTGGGGATGGATCTCACTTAGGTGTTCGTTTTACCTATGAGATGCAG
CCAAAACCAAACGGTCTCGCCGAAGCTTTTATTATTGGTAAAGATTTTATTGGAAAGGATTCTGTTTGTCTGGTGCTGGG
AGATAATATTTTTTATGGGAGCCATTTGAGCAATCTTTTAAAAAGTGCCAAAGAGAAAAAAGAGGGGGCTACTTTATTTG
GCTATGAGGTAAAAGATCCTGAAAGATATGGTGTCGTTGAATTTGATGGGCAGGGTAAAATTTTGTCGATCGAAGAAAAG
CCTAAAATTCCGAAATCTTCTATTGCTGTGACAGGTCTTTATTTTTATGATAATCAAGTCGTAGACATAGCTCATGGCTT
AAAACCCTCTGCACGAGGAGAATTAGAAATCACAGATGTCAACCAAGAGTATTTAAATAGAGATCAAGCATGTGTTCATG
TGATGGGAAGAGGATACACTTGGTTAGATGCAGGGACTTATGAAAGCCTTATGCAAGCAAGTCAGTTTGTTCAGGTGATT
GAGCAAAGACAAGGACACTGCATTGCTTCTTTAGAAGAAATTGCTTACAATCAAAAATTTATTTCTCAAGCCCAGTTAAA
ATTGTTGGGTGAAAAACTCGGGAAAAGCTTGTACGGACGTTATTTAATGGAAATTGCAGAAAAAGGACCTTACTATGGAA
ATCATTGA

Upstream 100 bases:

>100_bases
TGAATTTTCTACTCTAAAAATTCGGTACTGAATTAATATCGTCTGCAACAAAAAGCGCTCAATAGCATTCACTATCGGTA
GAAATTGTTAAGGGAGAAAT

Downstream 100 bases:

>100_bases
TTTGCAACTTAAAGGGTTAAAATTGGTGAAGCCTAACGTATTTAAAGATAATAGAGGTTTTTTTCTAGAAAGTTTTCAGC
AACCCCTCTATCAAAAAATG

Product: glucose-1-phosphate thymidylyltransferase (dTDP-glucose synthase), rfbA

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKGIVLAGGSGTRLHPLTLGVTKQLLPVYNKPMIYYPLSVLLLAEIKDILIISTPEDMPVFKRLLGDGSHLGVRFTYEMQ
PKPNGLAEAFIIGKDFIGKDSVCLVLGDNIFYGSHLSNLLKSAKEKKEGATLFGYEVKDPERYGVVEFDGQGKILSIEEK
PKIPKSSIAVTGLYFYDNQVVDIAHGLKPSARGELEITDVNQEYLNRDQACVHVMGRGYTWLDAGTYESLMQASQFVQVI
EQRQGHCIASLEEIAYNQKFISQAQLKLLGEKLGKSLYGRYLMEIAEKGPYYGNH

Sequences:

>Translated_295_residues
MKGIVLAGGSGTRLHPLTLGVTKQLLPVYNKPMIYYPLSVLLLAEIKDILIISTPEDMPVFKRLLGDGSHLGVRFTYEMQ
PKPNGLAEAFIIGKDFIGKDSVCLVLGDNIFYGSHLSNLLKSAKEKKEGATLFGYEVKDPERYGVVEFDGQGKILSIEEK
PKIPKSSIAVTGLYFYDNQVVDIAHGLKPSARGELEITDVNQEYLNRDQACVHVMGRGYTWLDAGTYESLMQASQFVQVI
EQRQGHCIASLEEIAYNQKFISQAQLKLLGEKLGKSLYGRYLMEIAEKGPYYGNH
>Mature_295_residues
MKGIVLAGGSGTRLHPLTLGVTKQLLPVYNKPMIYYPLSVLLLAEIKDILIISTPEDMPVFKRLLGDGSHLGVRFTYEMQ
PKPNGLAEAFIIGKDFIGKDSVCLVLGDNIFYGSHLSNLLKSAKEKKEGATLFGYEVKDPERYGVVEFDGQGKILSIEEK
PKIPKSSIAVTGLYFYDNQVVDIAHGLKPSARGELEITDVNQEYLNRDQACVHVMGRGYTWLDAGTYESLMQASQFVQVI
EQRQGHCIASLEEIAYNQKFISQAQLKLLGEKLGKSLYGRYLMEIAEKGPYYGNH

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=255, Percent_Identity=22.7450980392157, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI11761619, Length=255, Percent_Identity=22.7450980392157, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1788351, Length=286, Percent_Identity=60.1398601398601, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1790224, Length=290, Percent_Identity=58.2758620689655, Blast_Score=355, Evalue=2e-99,
Organism=Escherichia coli, GI1787488, Length=274, Percent_Identity=24.0875912408759, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI133931050, Length=248, Percent_Identity=24.1935483870968, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=272, Percent_Identity=27.2058823529412, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355443, Length=247, Percent_Identity=24.6963562753036, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24644084, Length=247, Percent_Identity=24.6963562753036, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32843; Mature: 32843

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIVLAGGSGTRLHPLTLGVTKQLLPVYNKPMIYYPLSVLLLAEIKDILIISTPEDMPV
CCCEEEECCCCCEEEEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCEEEEECCCCCHH
FKRLLGDGSHLGVRFTYEMQPKPNGLAEAFIIGKDFIGKDSVCLVLGDNIFYGSHLSNLL
HHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCEEEHHHHHHHH
KSAKEKKEGATLFGYEVKDPERYGVVEFDGQGKILSIEEKPKIPKSSIAVTGLYFYDNQV
HHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCEE
VDIAHGLKPSARGELEITDVNQEYLNRDQACVHVMGRGYTWLDAGTYESLMQASQFVQVI
EEHHHCCCCCCCCCEEEEECCHHHHCCHHHEEHHHCCCEEEECCCHHHHHHHHHHHHHHH
EQRQGHCIASLEEIAYNQKFISQAQLKLLGEKLGKSLYGRYLMEIAEKGPYYGNH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKGIVLAGGSGTRLHPLTLGVTKQLLPVYNKPMIYYPLSVLLLAEIKDILIISTPEDMPV
CCCEEEECCCCCEEEEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCEEEEECCCCCHH
FKRLLGDGSHLGVRFTYEMQPKPNGLAEAFIIGKDFIGKDSVCLVLGDNIFYGSHLSNLL
HHHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCEEEHHHHHHHH
KSAKEKKEGATLFGYEVKDPERYGVVEFDGQGKILSIEEKPKIPKSSIAVTGLYFYDNQV
HHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCEEEEEEEEECCEE
VDIAHGLKPSARGELEITDVNQEYLNRDQACVHVMGRGYTWLDAGTYESLMQASQFVQVI
EEHHHCCCCCCCCCEEEEECCHHHHCCHHHEEHHHCCCEEEECCCHHHHHHHHHHHHHHH
EQRQGHCIASLEEIAYNQKFISQAQLKLLGEKLGKSLYGRYLMEIAEKGPYYGNH
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]