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The map label for this gene is oppA [H]

Identifier: 46445737

GI number: 46445737

Start: 166774

End: 168921

Strand: Reverse

Name: oppA [H]

Synonym: pc0103

Alternate gene names: 46445737

Gene position: 168921-166774 (Counterclockwise)

Preceding gene: 46445738

Following gene: 46445736

Centisome position: 7.0

GC content: 36.08

Gene sequence:

>2148_bases
ATGAATAAAGAGCCTATTAGTTTATATATCTTCCGATTTATCTTAGGGTTTGGGTTATTCGCGTTCATGGCTATGTTGTA
TTGGTCATCTGCTCTGATTGAAAACCAGTTAAAATTTTTAAGACAAGACCTGATTCAAATCAAAAATGATATTTATACCG
TTCGTTCTGAAACAGAAAAAATTCGTACGGATATCTTAAAAACACTTATTAATCACCCTTTAAGCCAAAACAGTTCTTCA
TCTGAAACAGCCAATTTCCCTTTAACAATAAACAAAAAAGCTGCGCAGGGTGAAAATCTTCTTAAAGTAGATCCTTTTTA
TCAAATCACCCTCCCTCGATTACTAGGACCTCAATTTACCCCGCAGGGAGTTCGCAAAGAGGCAACGTTCAGAAAACCGG
ATCATCTCCACCCTTTTAGCCCCTGGTTAGAAGTTTCTTCATGGAACGATCTGTGCTCTATTTCATTAGCTTCCCAAGAA
GTGGGTAAATATGAAACAATGGCTCCAGAAATGGCTGAAAGAATAGAATTAAGAACTAATGAAAAAGGTCTTTCCGAGTA
TTGGGTATTTCTAAGGCAAGATGTATTTTGGGCTCCCCTTCAACAAAATCAATTTGCTGAAGGGATTATTTTAGCCCCTC
ATTTTTTACGCCGACACCCAGTCACTGCTCATGACTTTAAATTTTACTTTGACGCAATCATGAATCCATACGTGGAAGAA
GCCAAAGCCGTTTCCCTTAGAATTTACTTTGGAGATATTGAAGAAATTAAAATACTCGATGATTACACTTTCATTGTGCG
ATGGAAAACGGAAAAAACCGTAGGAGAAAATGGAAAAGAAGAACTAAAAATGAAATATCTGTCAAAATCTTGGACAGGAT
CTTTATGTCCTTTAGCTTCCTTTGTTTATCAGTATTTTGCGGATGGCACAAAAATTATTGAAGAAGATAGCCACCCTAAC
ACTTACCAAACTAATTCTATCTGGGCACAAAATTTTTCCCATCACTGGGCAAAAAATGTCATTGTCAGTTGTGGTCCGTG
GTTATTCGATGGCATGACCGAAAGGGAAATACGATTCAAAAGAAATCCTGATTATTTTAATGCTTATGCTGCCCTAACCG
AAGCGCTAGAATTCAAATTTAAAAACTCTCCAGATGGAATTTGGGAAGAATTTAAAACTGATTCTTTAGATTTATTTGAA
ATTCCTCCTAATCAGCTTGCAGAGTTAGATCGATTTTTACAAAGTGCCCCTTATGCCAAACAAAAAGAAAATGGATCGGG
AATTCATCGATTGGACTATATTAGTCGAAAATATTATTACATTGGATGGAACCAAGCTAACCCCTTATTTAAAAGCAAAA
AAGTTCGACAAGCTTTGACGATGGCTATTGATAGAGAACGCATTATTCGTCAAAATTTAAATGGAATGGGAATGCAAACA
ACGGGAACCTTTTTCCCTTTTTCTCCCTCCTATGACGCGACATTAAAACCCTATCCTTACAATATTCAAAAAGCTTTGGA
ATTATTAAGAGAAGATGGATGGTACGATAGTTCAGGAACGGGTGTCTTAGAAAAAGTCATTGACGGTCAACGCACCCCTT
TTAAATTTACATTGACATATTTTGTCAAAAGCCCAACAAATAAATCAATTTGTGAATACATTTCGACTGCTTTAAAAGAA
ATTGGGATTGTTTGTAAAATTGAGGGGGTAGATGTAGCAGATTTATCTGCTATAGTGGACGATAAAAGTTTTGATGCTAT
TTATATGGCTTGGGCTCTTGGATCGCCTCCTGAAGATCCAAGGCAGCTGTGGTATTCAACAGGAGCCAAAGAAAAAGGCT
CTTCAAATCTAATTGGTTTTGTCAATCAAGAAGTAGATCATATCATTGACAAATTAACTTTTGAATATGATGTCGAAAAA
CGTATTGAACTTTACCATCAATTTGATCGCATTATTTATGATGAAGCTCCCTATACATTTATTTATACTCCCAAAGAGAC
TCTTCTTTACCGCGATTACTTGCAAAATGTTTTCATCCCTGCTGAAAGGCAAGATTTAATCCCGGGAGCTAATGTAGGCG
AACCTATTGCAAACTTATTTTGGATTAAAGATACTTCTAAAAAACAAGAAAAATACCTTGGGAACTAA

Upstream 100 bases:

>100_bases
GAATTTTAGCTTGTTGTAGAGGATTTGCAGAACTGGGGTGGATTTCACATTTTGTCTTAAATCCTGACTCTTCACCCCTT
TATCATATAGTGATTTTCTA

Downstream 100 bases:

>100_bases
AAGATGTGGAATTATGTCTTAAGACGATTTCTTCTTCTTCCACTAACCCTTTTCTTTATTGTTTTAATAAATTTTATCAT
TATTAATCTCGCTCCAGGTG

Product: putative substrate binding proteins, component of oligopeptide permease, oppA

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MNKEPISLYIFRFILGFGLFAFMAMLYWSSALIENQLKFLRQDLIQIKNDIYTVRSETEKIRTDILKTLINHPLSQNSSS
SETANFPLTINKKAAQGENLLKVDPFYQITLPRLLGPQFTPQGVRKEATFRKPDHLHPFSPWLEVSSWNDLCSISLASQE
VGKYETMAPEMAERIELRTNEKGLSEYWVFLRQDVFWAPLQQNQFAEGIILAPHFLRRHPVTAHDFKFYFDAIMNPYVEE
AKAVSLRIYFGDIEEIKILDDYTFIVRWKTEKTVGENGKEELKMKYLSKSWTGSLCPLASFVYQYFADGTKIIEEDSHPN
TYQTNSIWAQNFSHHWAKNVIVSCGPWLFDGMTEREIRFKRNPDYFNAYAALTEALEFKFKNSPDGIWEEFKTDSLDLFE
IPPNQLAELDRFLQSAPYAKQKENGSGIHRLDYISRKYYYIGWNQANPLFKSKKVRQALTMAIDRERIIRQNLNGMGMQT
TGTFFPFSPSYDATLKPYPYNIQKALELLREDGWYDSSGTGVLEKVIDGQRTPFKFTLTYFVKSPTNKSICEYISTALKE
IGIVCKIEGVDVADLSAIVDDKSFDAIYMAWALGSPPEDPRQLWYSTGAKEKGSSNLIGFVNQEVDHIIDKLTFEYDVEK
RIELYHQFDRIIYDEAPYTFIYTPKETLLYRDYLQNVFIPAERQDLIPGANVGEPIANLFWIKDTSKKQEKYLGN

Sequences:

>Translated_715_residues
MNKEPISLYIFRFILGFGLFAFMAMLYWSSALIENQLKFLRQDLIQIKNDIYTVRSETEKIRTDILKTLINHPLSQNSSS
SETANFPLTINKKAAQGENLLKVDPFYQITLPRLLGPQFTPQGVRKEATFRKPDHLHPFSPWLEVSSWNDLCSISLASQE
VGKYETMAPEMAERIELRTNEKGLSEYWVFLRQDVFWAPLQQNQFAEGIILAPHFLRRHPVTAHDFKFYFDAIMNPYVEE
AKAVSLRIYFGDIEEIKILDDYTFIVRWKTEKTVGENGKEELKMKYLSKSWTGSLCPLASFVYQYFADGTKIIEEDSHPN
TYQTNSIWAQNFSHHWAKNVIVSCGPWLFDGMTEREIRFKRNPDYFNAYAALTEALEFKFKNSPDGIWEEFKTDSLDLFE
IPPNQLAELDRFLQSAPYAKQKENGSGIHRLDYISRKYYYIGWNQANPLFKSKKVRQALTMAIDRERIIRQNLNGMGMQT
TGTFFPFSPSYDATLKPYPYNIQKALELLREDGWYDSSGTGVLEKVIDGQRTPFKFTLTYFVKSPTNKSICEYISTALKE
IGIVCKIEGVDVADLSAIVDDKSFDAIYMAWALGSPPEDPRQLWYSTGAKEKGSSNLIGFVNQEVDHIIDKLTFEYDVEK
RIELYHQFDRIIYDEAPYTFIYTPKETLLYRDYLQNVFIPAERQDLIPGANVGEPIANLFWIKDTSKKQEKYLGN
>Mature_715_residues
MNKEPISLYIFRFILGFGLFAFMAMLYWSSALIENQLKFLRQDLIQIKNDIYTVRSETEKIRTDILKTLINHPLSQNSSS
SETANFPLTINKKAAQGENLLKVDPFYQITLPRLLGPQFTPQGVRKEATFRKPDHLHPFSPWLEVSSWNDLCSISLASQE
VGKYETMAPEMAERIELRTNEKGLSEYWVFLRQDVFWAPLQQNQFAEGIILAPHFLRRHPVTAHDFKFYFDAIMNPYVEE
AKAVSLRIYFGDIEEIKILDDYTFIVRWKTEKTVGENGKEELKMKYLSKSWTGSLCPLASFVYQYFADGTKIIEEDSHPN
TYQTNSIWAQNFSHHWAKNVIVSCGPWLFDGMTEREIRFKRNPDYFNAYAALTEALEFKFKNSPDGIWEEFKTDSLDLFE
IPPNQLAELDRFLQSAPYAKQKENGSGIHRLDYISRKYYYIGWNQANPLFKSKKVRQALTMAIDRERIIRQNLNGMGMQT
TGTFFPFSPSYDATLKPYPYNIQKALELLREDGWYDSSGTGVLEKVIDGQRTPFKFTLTYFVKSPTNKSICEYISTALKE
IGIVCKIEGVDVADLSAIVDDKSFDAIYMAWALGSPPEDPRQLWYSTGAKEKGSSNLIGFVNQEVDHIIDKLTFEYDVEK
RIELYHQFDRIIYDEAPYTFIYTPKETLLYRDYLQNVFIPAERQDLIPGANVGEPIANLFWIKDTSKKQEKYLGN

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=364, Percent_Identity=23.9010989010989, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789966, Length=291, Percent_Identity=25.7731958762887, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787052, Length=231, Percent_Identity=24.6753246753247, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 82989; Mature: 82989

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEPISLYIFRFILGFGLFAFMAMLYWSSALIENQLKFLRQDLIQIKNDIYTVRSETEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHH
IRTDILKTLINHPLSQNSSSSETANFPLTINKKAAQGENLLKVDPFYQITLPRLLGPQFT
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECHHHCCCCCC
PQGVRKEATFRKPDHLHPFSPWLEVSSWNDLCSISLASQEVGKYETMAPEMAERIELRTN
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCC
EKGLSEYWVFLRQDVFWAPLQQNQFAEGIILAPHFLRRHPVTAHDFKFYFDAIMNPYVEE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHCHHHHC
AKAVSLRIYFGDIEEIKILDDYTFIVRWKTEKTVGENGKEELKMKYLSKSWTGSLCPLAS
CCEEEEEEEECCCCEEEEEECEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
FVYQYFADGTKIIEEDSHPNTYQTNSIWAQNFSHHWAKNVIVSCGPWLFDGMTEREIRFK
HHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEC
RNPDYFNAYAALTEALEFKFKNSPDGIWEEFKTDSLDLFEIPPNQLAELDRFLQSAPYAK
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCC
QKENGSGIHRLDYISRKYYYIGWNQANPLFKSKKVRQALTMAIDRERIIRQNLNGMGMQT
CCCCCCCCHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
TGTFFPFSPSYDATLKPYPYNIQKALELLREDGWYDSSGTGVLEKVIDGQRTPFKFTLTY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
FVKSPTNKSICEYISTALKEIGIVCKIEGVDVADLSAIVDDKSFDAIYMAWALGSPPEDP
EEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCH
RQLWYSTGAKEKGSSNLIGFVNQEVDHIIDKLTFEYDVEKRIELYHQFDRIIYDEAPYTF
HHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
IYTPKETLLYRDYLQNVFIPAERQDLIPGANVGEPIANLFWIKDTSKKQEKYLGN
EECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHEEEECCCCHHHHHHCCC
>Mature Secondary Structure
MNKEPISLYIFRFILGFGLFAFMAMLYWSSALIENQLKFLRQDLIQIKNDIYTVRSETEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHH
IRTDILKTLINHPLSQNSSSSETANFPLTINKKAAQGENLLKVDPFYQITLPRLLGPQFT
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCEEEECHHHCCCCCC
PQGVRKEATFRKPDHLHPFSPWLEVSSWNDLCSISLASQEVGKYETMAPEMAERIELRTN
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCCC
EKGLSEYWVFLRQDVFWAPLQQNQFAEGIILAPHFLRRHPVTAHDFKFYFDAIMNPYVEE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHCCCCCHHHHHHHHHHHHCHHHHC
AKAVSLRIYFGDIEEIKILDDYTFIVRWKTEKTVGENGKEELKMKYLSKSWTGSLCPLAS
CCEEEEEEEECCCCEEEEEECEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
FVYQYFADGTKIIEEDSHPNTYQTNSIWAQNFSHHWAKNVIVSCGPWLFDGMTEREIRFK
HHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEC
RNPDYFNAYAALTEALEFKFKNSPDGIWEEFKTDSLDLFEIPPNQLAELDRFLQSAPYAK
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCCCC
QKENGSGIHRLDYISRKYYYIGWNQANPLFKSKKVRQALTMAIDRERIIRQNLNGMGMQT
CCCCCCCCHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
TGTFFPFSPSYDATLKPYPYNIQKALELLREDGWYDSSGTGVLEKVIDGQRTPFKFTLTY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEEE
FVKSPTNKSICEYISTALKEIGIVCKIEGVDVADLSAIVDDKSFDAIYMAWALGSPPEDP
EEECCCCHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCH
RQLWYSTGAKEKGSSNLIGFVNQEVDHIIDKLTFEYDVEKRIELYHQFDRIIYDEAPYTF
HHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEE
IYTPKETLLYRDYLQNVFIPAERQDLIPGANVGEPIANLFWIKDTSKKQEKYLGN
EECCHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHEEEECCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]