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The map label for this gene is pitB [C]

Identifier: 46445733

GI number: 46445733

Start: 161360

End: 162775

Strand: Direct

Name: pitB [C]

Synonym: pc0099

Alternate gene names: 46445733

Gene position: 161360-162775 (Clockwise)

Preceding gene: 46445732

Following gene: 46445734

Centisome position: 6.68

GC content: 37.22

Gene sequence:

>1416_bases
ATGGATACTGCGCTACTCATTTTGATTTTGATCACCGGATTTTATATGGCTTGGAGCATAGGAGCTAATGATGTTGCCAA
TGCTATGGGAACATCTGTTGGTTCAGGTTCATTAACTTTGCGCCAAGCTGTTCTGATTGCCGCTGTTTTAGAATTTTGCG
GCGCTTTTTTCTTTGGTTCCCATGTTTCTAAAACTGTGCAAAGTGGGATTATTGATCCAGATGTTTTTAATTATGATCCC
AAACTGCTAGTTTTTGGAATGCTTGCTTCTTTAGGTTCTGTAGGAATGTGGCTTCAATTAGCATCCTATTTTGGTTGGCC
TGTTTCAACGACACATTCAATTGTTGGCGCGATTGTAGGCTTTGGTACTGTTTTTGGAGGGATAAAGGCCGTATACTGGA
AAGAAGTGTGTTATATTATTACCAGTTGGATTTTTTCTCCTATTTTAGGAGGGATTATTGCTTACTATATTTTTTCTCTT
TTAAGAAAAAGAATTTTTTATGCTTTAAATCCTCTGGAAGAAACGCGTCGTTTAACACCATTTTTAGTTTTTGTGGTTAC
CATTATTCTAGCTGTGGTTCTTATTTTTGAAGGGCTGCATAATTTAAATGTAGAATTTTCGTTAACAAGTAAAATTTTGA
TGACACTTGCGATTGGCTTGATGGGTTCTTTCGTTAGTTATTTATTAGTACGCCGAATTCCTATTATTCCCCAAGAAAAA
CTCTCTGTTCCTTACAATTCTGAAGCTTTACATTCTTTGGAAAAGGTTCGTAAACATCTGCAAAGATTTAGAATGAAGTC
TGCTGGAGAAGCTCAATACACTGCAGGATTATTATTAGAAGAAGTAGAGGCCCTTTCAAAAACTTTTGAATCAAAAATTA
ATACAGATCATACACATGTTGAATATGTTCAAGTGGAAAAAATATTTGCTTATTTGCAAATTATGACTGCTTGTATGATG
GCTTTTGCACATGGAGCTAATGATGTTGCTAATGCTATAGGTCCTTTATCAGCTGCAGTAGCTATTTTGACAACTGGTTT
ATTTGCTGTGGATGCTCCCGTTCCTACTTGGGCTCTTGCATTAGGTGGAAGTGGAATTGTTATAGGGTTAGCAACCTGGG
GGTGGAGAGTTATTGAGACAATTGGAAAAAAAATTACAGAATTAACAGCTAGTCGAGGTTTTGCTGCTGAATTTGGAGCG
GCTACAACAATTGTCATTGCTTCTAGGTTTGGTTTACCTATTTCAACAACGCACACGCTTGTGGGTGCTGTTTTGGGAGT
GGGGTTTGCTAGAGGTCTTGAAGCAGTTAATTTAACAACGACCCGTGATATTTTAGTTTCTTGGATCGTGACGGTTCCTA
TTGGAGCTTTGTTAGCAATTATATTGATTTATCCGATCCAAGCAATATTTGGATAA

Upstream 100 bases:

>100_bases
CAGAAAACTTGGCAAATCGCGTTAGAATGGTTTTAGAATTGAAATAAATCTTATGACCATTTGTTGCTAAAGGAATAAGT
AAGAGATTCAATAAGGAGTT

Downstream 100 bases:

>100_bases
ATAGACAAGAGAATTGGTTTAATCGAAGGAGATAAAAATGTCTAAAATTTCTCTACCATCAACGCGCTTGTTATTTAAGC
TAGGGCTAGGCTTTTTAGCT

Product: hypothetical protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDTALLILILITGFYMAWSIGANDVANAMGTSVGSGSLTLRQAVLIAAVLEFCGAFFFGSHVSKTVQSGIIDPDVFNYDP
KLLVFGMLASLGSVGMWLQLASYFGWPVSTTHSIVGAIVGFGTVFGGIKAVYWKEVCYIITSWIFSPILGGIIAYYIFSL
LRKRIFYALNPLEETRRLTPFLVFVVTIILAVVLIFEGLHNLNVEFSLTSKILMTLAIGLMGSFVSYLLVRRIPIIPQEK
LSVPYNSEALHSLEKVRKHLQRFRMKSAGEAQYTAGLLLEEVEALSKTFESKINTDHTHVEYVQVEKIFAYLQIMTACMM
AFAHGANDVANAIGPLSAAVAILTTGLFAVDAPVPTWALALGGSGIVIGLATWGWRVIETIGKKITELTASRGFAAEFGA
ATTIVIASRFGLPISTTHTLVGAVLGVGFARGLEAVNLTTTRDILVSWIVTVPIGALLAIILIYPIQAIFG

Sequences:

>Translated_471_residues
MDTALLILILITGFYMAWSIGANDVANAMGTSVGSGSLTLRQAVLIAAVLEFCGAFFFGSHVSKTVQSGIIDPDVFNYDP
KLLVFGMLASLGSVGMWLQLASYFGWPVSTTHSIVGAIVGFGTVFGGIKAVYWKEVCYIITSWIFSPILGGIIAYYIFSL
LRKRIFYALNPLEETRRLTPFLVFVVTIILAVVLIFEGLHNLNVEFSLTSKILMTLAIGLMGSFVSYLLVRRIPIIPQEK
LSVPYNSEALHSLEKVRKHLQRFRMKSAGEAQYTAGLLLEEVEALSKTFESKINTDHTHVEYVQVEKIFAYLQIMTACMM
AFAHGANDVANAIGPLSAAVAILTTGLFAVDAPVPTWALALGGSGIVIGLATWGWRVIETIGKKITELTASRGFAAEFGA
ATTIVIASRFGLPISTTHTLVGAVLGVGFARGLEAVNLTTTRDILVSWIVTVPIGALLAIILIYPIQAIFG
>Mature_471_residues
MDTALLILILITGFYMAWSIGANDVANAMGTSVGSGSLTLRQAVLIAAVLEFCGAFFFGSHVSKTVQSGIIDPDVFNYDP
KLLVFGMLASLGSVGMWLQLASYFGWPVSTTHSIVGAIVGFGTVFGGIKAVYWKEVCYIITSWIFSPILGGIIAYYIFSL
LRKRIFYALNPLEETRRLTPFLVFVVTIILAVVLIFEGLHNLNVEFSLTSKILMTLAIGLMGSFVSYLLVRRIPIIPQEK
LSVPYNSEALHSLEKVRKHLQRFRMKSAGEAQYTAGLLLEEVEALSKTFESKINTDHTHVEYVQVEKIFAYLQIMTACMM
AFAHGANDVANAIGPLSAAVAILTTGLFAVDAPVPTWALALGGSGIVIGLATWGWRVIETIGKKITELTASRGFAAEFGA
ATTIVIASRFGLPISTTHTLVGAVLGVGFARGLEAVNLTTTRDILVSWIVTVPIGALLAIILIYPIQAIFG

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=156, Percent_Identity=49.3589743589744, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI5803173, Length=155, Percent_Identity=49.6774193548387, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1789360, Length=492, Percent_Identity=25.2032520325203, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25146401, Length=461, Percent_Identity=34.9240780911063, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17539280, Length=501, Percent_Identity=34.5309381237525, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI71981576, Length=465, Percent_Identity=35.2688172043011, Blast_Score=256, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI32566716, Length=471, Percent_Identity=33.3333333333333, Blast_Score=238, Evalue=7e-63,
Organism=Caenorhabditis elegans, GI17536725, Length=476, Percent_Identity=30.0420168067227, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17557328, Length=510, Percent_Identity=28.2352941176471, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319773, Length=160, Percent_Identity=43.125, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21356511, Length=233, Percent_Identity=34.7639484978541, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 50871; Mature: 50871

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]