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The map label for this gene is topA [H]

Identifier: 46445705

GI number: 46445705

Start: 128482

End: 131085

Strand: Reverse

Name: topA [H]

Synonym: pc0071

Alternate gene names: 46445705

Gene position: 131085-128482 (Counterclockwise)

Preceding gene: 46445706

Following gene: 46445704

Centisome position: 5.43

GC content: 37.86

Gene sequence:

>2604_bases
ATGGGTAAAGCGCTGATTATCGTCGAATCACCCGCTAAAATTAAAACGTTGAAAAAATTTCTTGGCACAAATTTTATTTT
TGAGTCGTCGATCGGCCATGTTCGCGATTTACCTGAAAGAGAATTTGGAATCGATATTGAGAATGACTTCGAACCTAAAT
ATACGATTATGCCTAACAAAGAAGAAGTCATTTCAAAGCTTTTAAAAGCAGCTAAACAGTGTGATGTCGTTTACCTTTCT
CCAGATCCTGATAGAGAAGGAGAAGCTATTGCTTGGCACATTACTCAAGTTCTTCCTCCTAATACAAATATTAAAAGAGT
CTCTTTCAATTCTATTACAAAAGATGCTGTTGTTAAGGCATTGGAAAATCCTAGAGAAATTGATATTGCCTTAGTAAATG
CCCAACAAGCGAGACGATTATTAGATCGCATTGTGGGATATAAAATTTCCCCTTTACTCAATAGACGAATTCAACGAGGG
CGAGAAAACTTCTTATCGGCTGGAAGGGTTCAGTCTGTTGCTTTAAAGTTAGTGGTGGATCGTGAAAAAGAAATTGAAGC
TTTTAAACCTATCGAATATTGGAATATCGGAGCCATTTTAAAAACTAATCAAGAAGATCGCCTTTTTCGAGCTGCATTGT
ACTCTGTCGATGGAAAACGATTTGAGAAAGAACCCATTGAAGGAAAAGAGATTACAATTATTAACAATAAAGAAGCAGCT
GACGCGATTTTAAAACGGATGAAACCCGGTCCTTATCAGGTCAAAAAAGTTGAACGTAAAGAAAAACGACGTTTTCCAGT
TCCTCCTTTTATCACTTCTACTCTTCAGCAAGAAGCTAGCCGACACCACGGTTTTTCTTCCGCTCGTACGATGAACATTG
CTCAAGGGCTTTATGAAGGTGTTGATCTTGGATCTGACGGACCAGAAGGTTTAATCACTTATATGCGTACAGACTCAGTT
CGCATTTCTCCAGAGGCCGTTCAAGAAGCGCGCGAATTCATAAAAAAGCAATATGGTAGCGATTTTATTCCATCGGATCC
TAAACAATACAGTACTCAAAAAAGCGCGCAAGATGCTCACGAAGCAATCAGGCCTGCCAGTTTGCAAAATACACCTGAAA
AAGTTCAGCCATACCTAACTAAAGAACAATTTTCATTGTACCAATTGATTTGGCGACGATTTTTAGCTTCTCAAATGGTC
GCTGCTATCTACGATACTGTTTCAGCTGATATTTTAGCTGGTGAAGGAATTTTACTCAGAGCGACAGGTTCGATTATCAA
ATTTCAGGGCTTTTTAGCTGTTTATGAAGAAAAAAATGATGACGACGAAAAAGATGATGAAAATCGAATGTTGCCTAAAC
TGGAAGAAGGACAAACGCTTTTCCTGCAAGAATTAACTTCCGAGCAAGCTTTCACAAGACCTCCGCCTCGTTTTACAGAA
GCTTCTTTAGTAAAAGAACTAGAAAAATCGGGTATTGGAAGACCATCGACTTACGCTGCAATTATGAATAAAATTCAAAG
TCGGGATTATACGGTTAAAGAAAACGGAAGGCTCAAACCCACGGAACTCGGTCAAATTATTGCTCAAATGTTAGAAACTA
GTTTTCAAAAAATCATGAATATTGGCTTTACCGCAGCTATGGAAGACGATTTAGAGCGTGTAGCCGAAAATATGAAAGAT
TGGAAAACCCTTATTCGAGATTTTTGGGAACAATTTAACCCCACTTTGGAAATAGCTCTTAAAGAAGCATTTGTTCCTAA
AGTCATGACCGAAATTGATTGCCCGAAATGTAAAATTGGCAAGCTACAAAAAGTTTGGGCTCGTTCTAAGTATTTCTTTG
GATGTTCTCGTTATCCTGAATGTGATTATTCTTCCCCTGTGGAAGAAATTACATTTAATAAAGAAGATTATGCTACTGAC
TTTGATTGGGAGCAGCCTTGCCCTAATTGTAACTCTGAAATGAAAATTAGACACGGACGTTACGGAGCATTTTTAGGTTG
TACAAAGTATCCTGAATGCAAAGGTATTATCAATATTCCTAAAAAAGGTGAGGAAGCGCTATCGCAGCAAGATCTTCCTT
CTTGCCCAGCTATCGAATGCCCCGGCCATATGGTAGCTCGAAAATCTCGTTTTGGTAAAATTTTTTATTCTTGCTCGACT
TTCCCCGAATGTGATGTCATTGTTAATAATTTAGAGCAGGTAGAAAATAAATATCCCAATCATCCGCGCACACCCTACGA
GAAAAAAGGAAAAAAAGCTGCAGCTAAAACAGCTACTAAAGAAAAAACAAAGAAAACTGCAAAAGCTAACGCTTCAAAAG
CGACTAAAAAAATCAAATCGACCCCCGATAAACCAAAGAAAGTAAGGCAAATGCCTGTTTATCAAGTTTCTCCTGAATTA
AGAGGGATTATAGAGGTTTCAGAAATAACCCGTGGCGACATGACGAAAAAAGTTTGGGATTATATTAAAACACACCAACT
TCAAGATACAAACAATAAACGGCTAATTATACCAGATGCCAAGCTTTCGCAAGTTTTTGGAACAACACAACCTGTAGACA
TGTTTAAAATGGCTACTTTATTAAGCGCTCATCTCAAAAAATAA

Upstream 100 bases:

>100_bases
ATTTTATAAAAAAATTTAATAGAATGAAGAGACATCAAAGAGAGCTTTAGTTTTACAATAAAATTTAACATATGCTCTTC
AATCACGGAGTTATGACAAC

Downstream 100 bases:

>100_bases
TTAAATGCCAGAAAAAGAAAACAAGAAGTTGGAGCGTAACGGAGTCGAACCGTTGACCTCAACAATGCCATTGTTGCGCT
CTACCAACTGAGCTAACGCC

Product: DNA topoisomerase I/SWI domain fusion protein

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 867; Mature: 866

Protein sequence:

>867_residues
MGKALIIVESPAKIKTLKKFLGTNFIFESSIGHVRDLPEREFGIDIENDFEPKYTIMPNKEEVISKLLKAAKQCDVVYLS
PDPDREGEAIAWHITQVLPPNTNIKRVSFNSITKDAVVKALENPREIDIALVNAQQARRLLDRIVGYKISPLLNRRIQRG
RENFLSAGRVQSVALKLVVDREKEIEAFKPIEYWNIGAILKTNQEDRLFRAALYSVDGKRFEKEPIEGKEITIINNKEAA
DAILKRMKPGPYQVKKVERKEKRRFPVPPFITSTLQQEASRHHGFSSARTMNIAQGLYEGVDLGSDGPEGLITYMRTDSV
RISPEAVQEAREFIKKQYGSDFIPSDPKQYSTQKSAQDAHEAIRPASLQNTPEKVQPYLTKEQFSLYQLIWRRFLASQMV
AAIYDTVSADILAGEGILLRATGSIIKFQGFLAVYEEKNDDDEKDDENRMLPKLEEGQTLFLQELTSEQAFTRPPPRFTE
ASLVKELEKSGIGRPSTYAAIMNKIQSRDYTVKENGRLKPTELGQIIAQMLETSFQKIMNIGFTAAMEDDLERVAENMKD
WKTLIRDFWEQFNPTLEIALKEAFVPKVMTEIDCPKCKIGKLQKVWARSKYFFGCSRYPECDYSSPVEEITFNKEDYATD
FDWEQPCPNCNSEMKIRHGRYGAFLGCTKYPECKGIINIPKKGEEALSQQDLPSCPAIECPGHMVARKSRFGKIFYSCST
FPECDVIVNNLEQVENKYPNHPRTPYEKKGKKAAAKTATKEKTKKTAKANASKATKKIKSTPDKPKKVRQMPVYQVSPEL
RGIIEVSEITRGDMTKKVWDYIKTHQLQDTNNKRLIIPDAKLSQVFGTTQPVDMFKMATLLSAHLKK

Sequences:

>Translated_867_residues
MGKALIIVESPAKIKTLKKFLGTNFIFESSIGHVRDLPEREFGIDIENDFEPKYTIMPNKEEVISKLLKAAKQCDVVYLS
PDPDREGEAIAWHITQVLPPNTNIKRVSFNSITKDAVVKALENPREIDIALVNAQQARRLLDRIVGYKISPLLNRRIQRG
RENFLSAGRVQSVALKLVVDREKEIEAFKPIEYWNIGAILKTNQEDRLFRAALYSVDGKRFEKEPIEGKEITIINNKEAA
DAILKRMKPGPYQVKKVERKEKRRFPVPPFITSTLQQEASRHHGFSSARTMNIAQGLYEGVDLGSDGPEGLITYMRTDSV
RISPEAVQEAREFIKKQYGSDFIPSDPKQYSTQKSAQDAHEAIRPASLQNTPEKVQPYLTKEQFSLYQLIWRRFLASQMV
AAIYDTVSADILAGEGILLRATGSIIKFQGFLAVYEEKNDDDEKDDENRMLPKLEEGQTLFLQELTSEQAFTRPPPRFTE
ASLVKELEKSGIGRPSTYAAIMNKIQSRDYTVKENGRLKPTELGQIIAQMLETSFQKIMNIGFTAAMEDDLERVAENMKD
WKTLIRDFWEQFNPTLEIALKEAFVPKVMTEIDCPKCKIGKLQKVWARSKYFFGCSRYPECDYSSPVEEITFNKEDYATD
FDWEQPCPNCNSEMKIRHGRYGAFLGCTKYPECKGIINIPKKGEEALSQQDLPSCPAIECPGHMVARKSRFGKIFYSCST
FPECDVIVNNLEQVENKYPNHPRTPYEKKGKKAAAKTATKEKTKKTAKANASKATKKIKSTPDKPKKVRQMPVYQVSPEL
RGIIEVSEITRGDMTKKVWDYIKTHQLQDTNNKRLIIPDAKLSQVFGTTQPVDMFKMATLLSAHLKK
>Mature_866_residues
GKALIIVESPAKIKTLKKFLGTNFIFESSIGHVRDLPEREFGIDIENDFEPKYTIMPNKEEVISKLLKAAKQCDVVYLSP
DPDREGEAIAWHITQVLPPNTNIKRVSFNSITKDAVVKALENPREIDIALVNAQQARRLLDRIVGYKISPLLNRRIQRGR
ENFLSAGRVQSVALKLVVDREKEIEAFKPIEYWNIGAILKTNQEDRLFRAALYSVDGKRFEKEPIEGKEITIINNKEAAD
AILKRMKPGPYQVKKVERKEKRRFPVPPFITSTLQQEASRHHGFSSARTMNIAQGLYEGVDLGSDGPEGLITYMRTDSVR
ISPEAVQEAREFIKKQYGSDFIPSDPKQYSTQKSAQDAHEAIRPASLQNTPEKVQPYLTKEQFSLYQLIWRRFLASQMVA
AIYDTVSADILAGEGILLRATGSIIKFQGFLAVYEEKNDDDEKDDENRMLPKLEEGQTLFLQELTSEQAFTRPPPRFTEA
SLVKELEKSGIGRPSTYAAIMNKIQSRDYTVKENGRLKPTELGQIIAQMLETSFQKIMNIGFTAAMEDDLERVAENMKDW
KTLIRDFWEQFNPTLEIALKEAFVPKVMTEIDCPKCKIGKLQKVWARSKYFFGCSRYPECDYSSPVEEITFNKEDYATDF
DWEQPCPNCNSEMKIRHGRYGAFLGCTKYPECKGIINIPKKGEEALSQQDLPSCPAIECPGHMVARKSRFGKIFYSCSTF
PECDVIVNNLEQVENKYPNHPRTPYEKKGKKAAAKTATKEKTKKTAKANASKATKKIKSTPDKPKKVRQMPVYQVSPELR
GIIEVSEITRGDMTKKVWDYIKTHQLQDTNNKRLIIPDAKLSQVFGTTQPVDMFKMATLLSAHLKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=671, Percent_Identity=26.2295081967213, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI4507635, Length=519, Percent_Identity=23.6994219653179, Blast_Score=111, Evalue=3e-24,
Organism=Escherichia coli, GI1787529, Length=822, Percent_Identity=38.0778588807786, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1788061, Length=550, Percent_Identity=24.5454545454545, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI48994931, Length=137, Percent_Identity=37.956204379562, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=602, Percent_Identity=25.4152823920266, Blast_Score=121, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=491, Percent_Identity=26.0692464358452, Blast_Score=118, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=572, Percent_Identity=23.2517482517483, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=665, Percent_Identity=25.4135338345865, Blast_Score=137, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98785; Mature: 98653

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALIIVESPAKIKTLKKFLGTNFIFESSIGHVRDLPEREFGIDIENDFEPKYTIMPNK
CCCEEEEEECCHHHHHHHHHHCCCCEEECCCCHHHCCCCHHCCCCCCCCCCCCEEECCCH
EEVISKLLKAAKQCDVVYLSPDPDREGEAIAWHITQVLPPNTNIKRVSFNSITKDAVVKA
HHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCHHHHHHHHHH
LENPREIDIALVNAQQARRLLDRIVGYKISPLLNRRIQRGRENFLSAGRVQSVALKLVVD
HCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
REKEIEAFKPIEYWNIGAILKTNQEDRLFRAALYSVDGKRFEKEPIEGKEITIINNKEAA
CHHHHHHHCCCCEECCCEEEECCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEEECCCHHH
DAILKRMKPGPYQVKKVERKEKRRFPVPPFITSTLQQEASRHHGFSSARTMNIAQGLYEG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
VDLGSDGPEGLITYMRTDSVRISPEAVQEAREFIKKQYGSDFIPSDPKQYSTQKSAQDAH
CCCCCCCCCHHEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAIRPASLQNTPEKVQPYLTKEQFSLYQLIWRRFLASQMVAAIYDTVSADILAGEGILLR
HHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
ATGSIIKFQGFLAVYEEKNDDDEKDDENRMLPKLEEGQTLFLQELTSEQAFTRPPPRFTE
ECCCEEEEHHHEEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHH
ASLVKELEKSGIGRPSTYAAIMNKIQSRDYTVKENGRLKPTELGQIIAQMLETSFQKIMN
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGFTAAMEDDLERVAENMKDWKTLIRDFWEQFNPTLEIALKEAFVPKVMTEIDCPKCKIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHH
KLQKVWARSKYFFGCSRYPECDYSSPVEEITFNKEDYATDFDWEQPCPNCNSEMKIRHGR
HHHHHHHCCHHEECCCCCCCCCCCCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCC
YGAFLGCTKYPECKGIINIPKKGEEALSQQDLPSCPAIECPGHMVARKSRFGKIFYSCST
CCEEECCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCEEEEECCC
FPECDVIVNNLEQVENKYPNHPRTPYEKKGKKAAAKTATKEKTKKTAKANASKATKKIKS
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPDKPKKVRQMPVYQVSPELRGIIEVSEITRGDMTKKVWDYIKTHQLQDTNNKRLIIPDA
CCCCCHHHHHCCCEEECHHHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCH
KLSQVFGTTQPVDMFKMATLLSAHLKK
HHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKALIIVESPAKIKTLKKFLGTNFIFESSIGHVRDLPEREFGIDIENDFEPKYTIMPNK
CCEEEEEECCHHHHHHHHHHCCCCEEECCCCHHHCCCCHHCCCCCCCCCCCCEEECCCH
EEVISKLLKAAKQCDVVYLSPDPDREGEAIAWHITQVLPPNTNIKRVSFNSITKDAVVKA
HHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCHHHHHHHHHH
LENPREIDIALVNAQQARRLLDRIVGYKISPLLNRRIQRGRENFLSAGRVQSVALKLVVD
HCCCCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHC
REKEIEAFKPIEYWNIGAILKTNQEDRLFRAALYSVDGKRFEKEPIEGKEITIINNKEAA
CHHHHHHHCCCCEECCCEEEECCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEEECCCHHH
DAILKRMKPGPYQVKKVERKEKRRFPVPPFITSTLQQEASRHHGFSSARTMNIAQGLYEG
HHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
VDLGSDGPEGLITYMRTDSVRISPEAVQEAREFIKKQYGSDFIPSDPKQYSTQKSAQDAH
CCCCCCCCCHHEEEEECCCEEECHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
EAIRPASLQNTPEKVQPYLTKEQFSLYQLIWRRFLASQMVAAIYDTVSADILAGEGILLR
HHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEE
ATGSIIKFQGFLAVYEEKNDDDEKDDENRMLPKLEEGQTLFLQELTSEQAFTRPPPRFTE
ECCCEEEEHHHEEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHH
ASLVKELEKSGIGRPSTYAAIMNKIQSRDYTVKENGRLKPTELGQIIAQMLETSFQKIMN
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
IGFTAAMEDDLERVAENMKDWKTLIRDFWEQFNPTLEIALKEAFVPKVMTEIDCPKCKIG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCCHH
KLQKVWARSKYFFGCSRYPECDYSSPVEEITFNKEDYATDFDWEQPCPNCNSEMKIRHGR
HHHHHHHCCHHEECCCCCCCCCCCCCHHHHCCCCHHCCCCCCCCCCCCCCCCCCEEECCC
YGAFLGCTKYPECKGIINIPKKGEEALSQQDLPSCPAIECPGHMVARKSRFGKIFYSCST
CCEEECCCCCCCCCCEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHCEEEEECCC
FPECDVIVNNLEQVENKYPNHPRTPYEKKGKKAAAKTATKEKTKKTAKANASKATKKIKS
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPDKPKKVRQMPVYQVSPELRGIIEVSEITRGDMTKKVWDYIKTHQLQDTNNKRLIIPDA
CCCCCHHHHHCCCEEECHHHHHHEEHHHHHCCHHHHHHHHHHHHHCCCCCCCCEEEECCH
KLSQVFGTTQPVDMFKMATLLSAHLKK
HHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]