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The map label for this gene is trmE

Identifier: 46445656

GI number: 46445656

Start: 33359

End: 34735

Strand: Reverse

Name: trmE

Synonym: pc0022

Alternate gene names: 46445656

Gene position: 34735-33359 (Counterclockwise)

Preceding gene: 46445658

Following gene: 46445655

Centisome position: 1.44

GC content: 37.4

Gene sequence:

>1377_bases
ATGGAATTTATTCATCAACCCTATTACCCAGGTGAAACAATTGCAGCAATTGCAACCCCTCCAGGTGAGGGAGGAGTAGC
GATTATCCGCATTTCAGGCGATCAATCTCTTGAAGTAGCTGCTAAAATTTTTTCAGGCCCCATTTTCAGCTATCGCTCAC
ATACTGCACATTACGGGCAAATTTATAATTCATCAGGCGAACATGTCGATGATGTTTTGGTCCTTATAATGCTTGGTAAA
CGCTCTTATACAGGAGAAAATACTGTAGAAATTCATTGTCACGGAGGAAGTTTAATTACTCGCAAAGTTTTAGAAGTCGT
TTTAGCAGCCGGAGCTCGAGCAGCATTGCCTGGTGAATTTACTTTTAAAGCTTACATGAATGGTAAAATTGATTTAGCTC
AAGCCGAAGCTGTTCAAGAACTCATTTGTGCAAAAAATGAAAAAGCCCTAGGCGCGGCAGAAAGTCAACTAAAAGGTTCT
TTATCAAACCGGGTATTGGCCTTTCAATCAACCTTAACTCAAATTGCAGCGATTTTAGAAGCCTGGGTTGACTTTCCTGA
AGAAGGATTAGAATTTGCCACCATGGATGAATTGGATCAAGACTTAGAAAGGACTGCTAAAGACATGGAAAAGTTAGTGA
ATTCTTTTCACAATGGAAAAATTTTACATGATGGCCTTTCTATTTGCTTGATTGGATGCCCAAATGTAGGTAAGTCTTCT
TTGATGAACGCTCTGCTAGATAAGGATCGAGCTATTGTTTCCCCCATTCCTGGCACCACTCGAGATGTTTTAGAAGACCA
TCTACGTCTGAATGGACTACACATCAAATTGAGTGATACGGCAGGGATTAGAGAAGCTAATGAAAGCGTTGAACAAGAAG
GCATTCGTCGTTCTAAAAAAGCTATGCAAGAAGCCGATTTAATTTTACTCGTTTTAGATGCGCATAAAGGATTGGAAAAA
GAAGATCAGGAATTATTAAAACAAGTCCCTTTTCATAAAACAATCGTCATTTGGAATAAAATTGATCTCAATCCAAGAAA
TCTTCCTTGCTTAGAGGTTCCTTTTCTTGTTCATTTATCTGCTAAAGAAAAAATAGGATTAGAAGAGCTGCATCAAACAA
TTGATACCATTATTTGGCAAGATGGCCCTCCTTCCAAAGAAGAAATCCTTATTACAAATGTTAGACATAAAGAAGCTCTT
ATCGAATCGATTGAATCTTTAAGACGTGTAAAAATAGGTCTTCGACATCAAGTTTCACCTGAATTTCTAACTTTAGATAT
GCGCCAATCTTTATTAGAACTCGGGAAAATCATTGGAACTAATATCTCTGAAGATATTCTATCAGCAATTTTTTCTAAAT
TTTGTATAGGAAAATGA

Upstream 100 bases:

>100_bases
CGATGCTAATTGATAATCAAATAGATGCATCAAAAATAGATTTAATATAACATATTTCTCATTGTTATATCAAGAATCAC
AAATACAAATTTAAAGCATC

Downstream 100 bases:

>100_bases
ATAAGCATACAATAGCAAAAAATATTCAGCGTATTCTCAATGAACTTTACCCAGCTCCTGCAGTTCCTTTAAGTCATCAT
GACAGCTATACCTTACTTAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MEFIHQPYYPGETIAAIATPPGEGGVAIIRISGDQSLEVAAKIFSGPIFSYRSHTAHYGQIYNSSGEHVDDVLVLIMLGK
RSYTGENTVEIHCHGGSLITRKVLEVVLAAGARAALPGEFTFKAYMNGKIDLAQAEAVQELICAKNEKALGAAESQLKGS
LSNRVLAFQSTLTQIAAILEAWVDFPEEGLEFATMDELDQDLERTAKDMEKLVNSFHNGKILHDGLSICLIGCPNVGKSS
LMNALLDKDRAIVSPIPGTTRDVLEDHLRLNGLHIKLSDTAGIREANESVEQEGIRRSKKAMQEADLILLVLDAHKGLEK
EDQELLKQVPFHKTIVIWNKIDLNPRNLPCLEVPFLVHLSAKEKIGLEELHQTIDTIIWQDGPPSKEEILITNVRHKEAL
IESIESLRRVKIGLRHQVSPEFLTLDMRQSLLELGKIIGTNISEDILSAIFSKFCIGK

Sequences:

>Translated_458_residues
MEFIHQPYYPGETIAAIATPPGEGGVAIIRISGDQSLEVAAKIFSGPIFSYRSHTAHYGQIYNSSGEHVDDVLVLIMLGK
RSYTGENTVEIHCHGGSLITRKVLEVVLAAGARAALPGEFTFKAYMNGKIDLAQAEAVQELICAKNEKALGAAESQLKGS
LSNRVLAFQSTLTQIAAILEAWVDFPEEGLEFATMDELDQDLERTAKDMEKLVNSFHNGKILHDGLSICLIGCPNVGKSS
LMNALLDKDRAIVSPIPGTTRDVLEDHLRLNGLHIKLSDTAGIREANESVEQEGIRRSKKAMQEADLILLVLDAHKGLEK
EDQELLKQVPFHKTIVIWNKIDLNPRNLPCLEVPFLVHLSAKEKIGLEELHQTIDTIIWQDGPPSKEEILITNVRHKEAL
IESIESLRRVKIGLRHQVSPEFLTLDMRQSLLELGKIIGTNISEDILSAIFSKFCIGK
>Mature_458_residues
MEFIHQPYYPGETIAAIATPPGEGGVAIIRISGDQSLEVAAKIFSGPIFSYRSHTAHYGQIYNSSGEHVDDVLVLIMLGK
RSYTGENTVEIHCHGGSLITRKVLEVVLAAGARAALPGEFTFKAYMNGKIDLAQAEAVQELICAKNEKALGAAESQLKGS
LSNRVLAFQSTLTQIAAILEAWVDFPEEGLEFATMDELDQDLERTAKDMEKLVNSFHNGKILHDGLSICLIGCPNVGKSS
LMNALLDKDRAIVSPIPGTTRDVLEDHLRLNGLHIKLSDTAGIREANESVEQEGIRRSKKAMQEADLILLVLDAHKGLEK
EDQELLKQVPFHKTIVIWNKIDLNPRNLPCLEVPFLVHLSAKEKIGLEELHQTIDTIIWQDGPPSKEEILITNVRHKEAL
IESIESLRRVKIGLRHQVSPEFLTLDMRQSLLELGKIIGTNISEDILSAIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=33.974358974359, Blast_Score=212, Evalue=5e-55,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=33.2603938730853, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=32.2, Blast_Score=195, Evalue=6e-50,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=35.7456140350877, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI87082120, Length=104, Percent_Identity=35.5769230769231, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=458, Percent_Identity=30.7860262008734, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6323665, Length=507, Percent_Identity=30.1775147928994, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=30.9473684210526, Blast_Score=194, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PARUW (Q6MFA3)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007021.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q6MFA3
- SMR:   Q6MFA3
- STRING:   Q6MFA3
- GeneID:   2779712
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0022
- NMPDR:   fig|264201.1.peg.22
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q6MFA3
- ProtClustDB:   PRK05291
- BioCyc:   CPRO264201:PC0022-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50567; Mature: 50567

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 90-90 BINDING 129-129 BINDING 458-458

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFIHQPYYPGETIAAIATPPGEGGVAIIRISGDQSLEVAAKIFSGPIFSYRSHTAHYGQ
CCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCHHHH
IYNSSGEHVDDVLVLIMLGKRSYTGENTVEIHCHGGSLITRKVLEVVLAAGARAALPGEF
HCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCE
TFKAYMNGKIDLAQAEAVQELICAKNEKALGAAESQLKGSLSNRVLAFQSTLTQIAAILE
EEEEEECCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWVDFPEEGLEFATMDELDQDLERTAKDMEKLVNSFHNGKILHDGLSICLIGCPNVGKSS
HHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCHHH
LMNALLDKDRAIVSPIPGTTRDVLEDHLRLNGLHIKLSDTAGIREANESVEQEGIRRSKK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AMQEADLILLVLDAHKGLEKEDQELLKQVPFHKTIVIWNKIDLNPRNLPCLEVPFLVHLS
HHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEECCEEEEEC
AKEKIGLEELHQTIDTIIWQDGPPSKEEILITNVRHKEALIESIESLRRVKIGLRHQVSP
CHHCCCHHHHHHHHHHHEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLTLDMRQSLLELGKIIGTNISEDILSAIFSKFCIGK
HHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFIHQPYYPGETIAAIATPPGEGGVAIIRISGDQSLEVAAKIFSGPIFSYRSHTAHYGQ
CCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHCCCCCHHHH
IYNSSGEHVDDVLVLIMLGKRSYTGENTVEIHCHGGSLITRKVLEVVLAAGARAALPGEF
HCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCE
TFKAYMNGKIDLAQAEAVQELICAKNEKALGAAESQLKGSLSNRVLAFQSTLTQIAAILE
EEEEEECCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWVDFPEEGLEFATMDELDQDLERTAKDMEKLVNSFHNGKILHDGLSICLIGCPNVGKSS
HHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCCCHHH
LMNALLDKDRAIVSPIPGTTRDVLEDHLRLNGLHIKLSDTAGIREANESVEQEGIRRSKK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
AMQEADLILLVLDAHKGLEKEDQELLKQVPFHKTIVIWNKIDLNPRNLPCLEVPFLVHLS
HHHHCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEECCEEEEEC
AKEKIGLEELHQTIDTIIWQDGPPSKEEILITNVRHKEALIESIESLRRVKIGLRHQVSP
CHHCCCHHHHHHHHHHHEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
EFLTLDMRQSLLELGKIIGTNISEDILSAIFSKFCIGK
HHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA