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The map label for this gene is nth [H]

Identifier: 46445655

GI number: 46445655

Start: 32733

End: 33374

Strand: Reverse

Name: nth [H]

Synonym: pc0021

Alternate gene names: 46445655

Gene position: 33374-32733 (Counterclockwise)

Preceding gene: 46445656

Following gene: 46445651

Centisome position: 1.38

GC content: 37.85

Gene sequence:

>642_bases
TTGTATAGGAAAATGAATAAGCATACAATAGCAAAAAATATTCAGCGTATTCTCAATGAACTTTACCCAGCTCCTGCAGT
TCCTTTAAGTCATCATGACAGCTATACCTTACTTATCGCTGTTCTTTTATCCGCTCATTGCACCGATGCACGAGTTAATA
AAGTAACCCCTATTTTATTTAAAAAAGCAAGCACTCCCCAAGAAATGGTGAAACTATCCATTAATGAAATTGAATCTATT
ATCCATTCATGTGGACTCGGATTTCGTAAAGCGACAAATATCTGGGAACTTTCGGATCGGCTAATCAAAGATTATGAAGG
AAAAGTTCCGGCTTCATTTGAAGCATTAGAGTCGCTACCTGGGGTCGGACATAAAACAGCTTCTGTCGTCATGTCTCAAG
CATTTCAGGAAGCCGCTTTCCCTGTGGATACTCATATTCACCGTTGCGCTCGAAGATGGGGATTAAGCAATGGGAAAAAT
GTTAAACAAACAGAAAAAGATTTAAAATCTCTTTTTCCCAAAAAAGATTGGACTCGTCTTCATTTACAAATCATTTATTT
TGCAAGAGAACATTGTCAAGCCCGCTCACATCAAACTCCCATTTGCCCTATTTGTTCTTGGATAGTAGAAAATCCAATTT
GA

Upstream 100 bases:

>100_bases
AATTTCTAACTTTAGATATGCGCCAATCTTTATTAGAACTCGGGAAAATCATTGGAACTAATATCTCTGAAGATATTCTA
TCAGCAATTTTTTCTAAATT

Downstream 100 bases:

>100_bases
TATCCTAGGCATAAGCTCCAATTCATACGTGTTTCAAAGCAACGGTAATTAATTGAGCTAAGTCAATTTCTTCTGGAAGT
TCTTTCATCCCTTGTTTAAT

Product: endonuclease III (UV endonuclease)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MYRKMNKHTIAKNIQRILNELYPAPAVPLSHHDSYTLLIAVLLSAHCTDARVNKVTPILFKKASTPQEMVKLSINEIESI
IHSCGLGFRKATNIWELSDRLIKDYEGKVPASFEALESLPGVGHKTASVVMSQAFQEAAFPVDTHIHRCARRWGLSNGKN
VKQTEKDLKSLFPKKDWTRLHLQIIYFAREHCQARSHQTPICPICSWIVENPI

Sequences:

>Translated_213_residues
MYRKMNKHTIAKNIQRILNELYPAPAVPLSHHDSYTLLIAVLLSAHCTDARVNKVTPILFKKASTPQEMVKLSINEIESI
IHSCGLGFRKATNIWELSDRLIKDYEGKVPASFEALESLPGVGHKTASVVMSQAFQEAAFPVDTHIHRCARRWGLSNGKN
VKQTEKDLKSLFPKKDWTRLHLQIIYFAREHCQARSHQTPICPICSWIVENPI
>Mature_213_residues
MYRKMNKHTIAKNIQRILNELYPAPAVPLSHHDSYTLLIAVLLSAHCTDARVNKVTPILFKKASTPQEMVKLSINEIESI
IHSCGLGFRKATNIWELSDRLIKDYEGKVPASFEALESLPGVGHKTASVVMSQAFQEAAFPVDTHIHRCARRWGLSNGKN
VKQTEKDLKSLFPKKDWTRLHLQIIYFAREHCQARSHQTPICPICSWIVENPI

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=174, Percent_Identity=27.0114942528736, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=207, Percent_Identity=39.6135265700483, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17554540, Length=203, Percent_Identity=28.5714285714286, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324530, Length=179, Percent_Identity=23.463687150838, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=25.5555555555556, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24220; Mature: 24220

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRKMNKHTIAKNIQRILNELYPAPAVPLSHHDSYTLLIAVLLSAHCTDARVNKVTPILF
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHH
KKASTPQEMVKLSINEIESIIHSCGLGFRKATNIWELSDRLIKDYEGKVPASFEALESLP
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
GVGHKTASVVMSQAFQEAAFPVDTHIHRCARRWGLSNGKNVKQTEKDLKSLFPKKDWTRL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHH
HLQIIYFAREHCQARSHQTPICPICSWIVENPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MYRKMNKHTIAKNIQRILNELYPAPAVPLSHHDSYTLLIAVLLSAHCTDARVNKVTPILF
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCHHHH
KKASTPQEMVKLSINEIESIIHSCGLGFRKATNIWELSDRLIKDYEGKVPASFEALESLP
HCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
GVGHKTASVVMSQAFQEAAFPVDTHIHRCARRWGLSNGKNVKQTEKDLKSLFPKKDWTRL
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHH
HLQIIYFAREHCQARSHQTPICPICSWIVENPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]